scholarly journals Effect of Periodontal Surgery on Osteoprotegerin Levels in Gingival Crevicular Fluid, Saliva, and Gingival Tissues of Chronic Periodontitis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sandy H. S. Hassan ◽  
Mahmoud I. El-Refai ◽  
Noha A. Ghallab ◽  
Rehab Fawzy Kasem ◽  
Olfat G. Shaker
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Prognostic Biomarker ◽  
Bone Destruction ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Attachment Level

Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF), saliva, and gingival tissues of chronic periodontitis (CP) patients in response to open flap debridement (OFD).Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry.Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P<0.001). Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P<0.001) compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased.Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered withNCT02160613.

A Comparative Evaluation of Biphasic Calcium Phosphate Material and Bioglass in the Treatment of Periodontal Osseous Defects: A Clinical and Radiological Study

2010 ◽  
Vol 11 (2) ◽  
pp. 25-32 ◽  
Author(s):  
Rajan Gupta ◽  
Nymphea Pandit
Keyword(s):  
Calcium Phosphate ◽  
Comparative Evaluation ◽  
Biphasic Calcium Phosphate ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Radiological Study ◽  
Osseous Defects ◽  
Open Flap Debridement ◽  
Attachment Level

Abstract Aim The aim of this study was to evaluate the efficacy of biphasic calcium phosphate (ossifi®) and bioactive glass in the treatment of periodontal osseous defects clinically and radiographically and compare them with open-flap debridement. Methods and Materials A total of 45 sites in two test groups (test 1, ossifi; test 2, bioactive glass) and a control group (open-flap debridement), in 35 patients, were selected in this study conducted at the department of Periodontics and Oral Implantology, D.A.V.(C) Dental College, Yamuna Nagar, India. Clinical parameters like plaque index, gingival index, pocket depth, and clinical attachment level were recorded at the baseline and at three months and six months postoperatively. Radiological parameters like the amount of defect resolution and the percentage of defect resolution were recorded at the baseline and at three months and six months postoperatively. Results Statistically significant difference in mean values of the plaque index, gingival index, pocket depth reduction, clinical attachment level, gain amount of defect resolution, and percentage of defect resolution were observed in all the groups at subsequent time periods. Conclusion Both test groups showed significant improvement over the control in both the clinical and radiological parameters. Clinical Significance A greater percentage of defect resolution was noticed in test 1 as compared to test 2, followed by the control. Citation Pandit N, Gupta R, Gupta S. A Comparative Evaluation of Biphasic Calcium Phosphate Material and Bioglass in the Treatment of Periodontal Osseous Defects: A Clinical and Radiological Study. J Contemp Dent Pract [Internet]. 2010 March; 11(2):025-032. Available from: http://www.thejcdp.com/journal/view/ volume11-issue2-pandit.

scholarly journals Effectiveness of non-surgical treatment to reduce il-18 levels in the gingival crevicular fluid of patients with periodontal disease

2012 ◽  
Vol 23 (4) ◽  
pp. 428-432 ◽  
Author(s):  
Bernardo Oliveira de Campos ◽  
Ricardo Guimarães Fischer ◽  
Anders Gustafsson ◽  
Carlos Marcelo da Silva Figueredo
Keyword(s):  
Surgical Treatment ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Crevicular Fluid ◽  
Attachment Level ◽  
Gingival Fluid ◽  
Non Surgical Treatment

The aim of this study was to evaluate the effectiveness of the non-surgical periodontal treatment in reducing the gingival crevicular fluid (GCF) levels of IL-18 from inflamed periodontal sites. Fourteen patients with periodontal disease were included, being 9 patients with chronic periodontitis (mean age: 48.8 SD ± 7.4 years) and 5 patients with gingivitis (mean age: 43.6 SD ± 11.8). The patients were divided in the following groups: gingivitis sites from periodontitis patients (sites GP), periodontitis sites from periodontitis patients (sites PP), and gingivitis sites from gingivitis patients (sites GG). Probing pocket depth (PPD), probing attachment level (AL), plaque index (PI) and gingival index (GI) were recorded, and gingival fluid samples were collected. The subjects received non-surgical treatment and were re-evaluated 30 days after treatment (day 30 AT). There was a significant reduction in PI in GG (1.0 ± 0.4 to 0.5 ± 0.2), GP (1.2 ± 0.3 to 0.5 ± 0.3), and in PP (1.3 ± 0.4 to 0.7 ± 0.3) 30 AT. There was also a significant reduction in the GI in GG (1.3 ± 0.3 to 0.7 ± 0.4). PPD reduced significantly in GG (2.4 ± 0.6 to 1.9 ± 0.1), and PP (6.7 ± 1.1 to 5.2 ± 0.9) 30 AT. When all the samples were analyzed together, there was a significant reduction in IL-18 (12.9 ± 7.2 to 10.0 ± 3.1). This study showed that non-surgical treatment was effective in reducing GCF levels of IL-18 from inflamed periodontal sites.

NovaboneTM dental putty as a potential regenerative material for treating horizontal defects in chronic periodontitis patients.

2019 ◽  
Vol 11 (2) ◽  
pp. 2-10
Author(s):  
Dr. Shruthi Raveendran ◽  
Dr. Shruthi S ◽  
Dr. Nisha K J ◽  
Dr. Sanjeela Guru ◽  
Dr. Parichaya Batra ◽  
...  
Keyword(s):  
Bone Defects ◽  
Plaque Index ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Probing Pocket Depth ◽  
Calcium Phosphosilicate ◽  
Bone Level ◽  
Open Flap Debridement ◽  
Attachment Level

ntroduction: Periodontitis is a multifactorial disease, which, when not adequately treated, is followed with progressive attachment loss which leads to tooth mobility and eventually tooth loss. Periodontal regenerative surgery aims to regenerate and reconstruct the lost periodontal tissue. Regeneration with novabone putty has shown to be effective in reducing probing pocket depth, gain in clinical attachment level and increase in horizontal bone level. Aim: This interventional clinical trial was to evaluate the osseous regenerative potential of a calcium phosphosilicate bioactive glass NOVABONE TM in the treatment of horizontal bone defects. Materials and method: A total of 20 sites with horizontal bone defect was treated with open flap debridement with intra marrow penetration and novabone putty. Statistical analysis: Plaque index, gingival index and radiographic determination were analysed by paired t test. Probing pocket depth and clinical attachment levels were analysed by Wilcoxon Signed Ranks Test. Result: There was significant reduction seen in plaque index, gingival index, probing pocket depth and radiographic crestal level when compared between baseline and 12 months respectively (2.48 ± 0.44, 2.92 ± 0.39, 6.80 ± 0.89, 8.29 ± 0.87) and (1.89 ± 0.42, 1.92± 0.51, 3.00± 0.67, 5.48 ± 0.89) and significant gain in clinical attachment level from 4.40 ± 0.96 to 1.20 ± 0.91. Conclusion: The present study showed novabone putty significantly improved the clinical parameters in horizontal bone defects.

scholarly journals Clinical and Biochemical Assessment of Lycopene Gel Combined With Nanohydroxyapatite Graft in Treatment of Grade II Furcation Defects: A Randomized Controlled Clinical Study

Perio J ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 28-37
Author(s):  
Dalia Shawky Gaber ◽  
Nevine Hassan Kheir El Din ◽  
Hamdy Ahmed Nassar ◽  
Mostafa Saad El-Din Ashmawy ◽  
Ola Mohamed Ezzatt
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Clinical Attachment Level ◽  
Randomized Controlled ◽  
Furcation Defects ◽  
Grade Ii ◽  
Crevicular Fluid ◽  
Controlled Clinical Study ◽  
Open Flap Debridement ◽  
Attachment Level

Background: This study aimed to evaluate the effects of lycopene gel, as a natural antioxidant, mixed with a nanohydroxyapatite graft (NHG) covered by an occlusive resorbable collagen membrane (CM) in the surgical treatment of grade II furcation defects and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), as a marker of oxidative injury. Methods: In this randomized controlled clinical study a total of 24 patients with grade II furcation defects were randomly assigned into three equal groups. Furcation defects in group I were managed with lycopene gel mixed with NHG and CM, group II with NHG and CM, and group III with open flap debridement only. Site-specific changes in clinical parameters including probing depth (PD), vertical clinical attachment level (VCAL), horizontal clinical attachment level (HCAL), radiographic maximum vertical depth (MAX V), and maximum horizontal depth (MAX H) were measured at baseline and six months postoperatively. Gingival crevicular fluid levels of 8-OHdG were analyzed using enzyme-linked immunosorbent assay (ELISA) at baseline, one week, and three months. Results: Surgical management of grade II furcation defects resulted in a significant reduction in PD and 8-OHdG levels and a gain in CAL, MAX V, and MAX H in all groups. The differences between lycopene treated sites compared to NHG and CM alone were not significant at six months but demonstrated significantly superior clinical parameters compared to open flap debridement alone. Conclusion: Lycopene does not confer a benefit when combined with NHG in the surgical treatment of grade II furcation defects.

Role of Interleukin- 34 together with Receptor Activator of Nuclear Factor kB ligand and Osteoprotegrin levels in Periodontal Disease

2021 ◽  
Vol 15 (7) ◽  
pp. 1521-1522
Author(s):  
Noor Ul Amin ◽  
I. Saleem Qureshi ◽  
Sana Naeem ◽  
S. Hassan Jan ◽  
Kamal Khan ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Control Group ◽  
Gingival Index ◽  
Disease Group ◽  
Clinical Attachment Level ◽  
Group I ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Attachment Level

Aim: To compare the levels and relative ratios of Interleukin-34, osteoprotegrin, and factor kB ligand in the crevicular fluid of individuals with periodontal disease. Study design & setting: Randomized Clinical Trial, Frontier Medical And Dental College, Abbotabad Methodology: We enrolled 15 healthy subjects (Group I), 15 patients with chronic periodontal disease (Group II) and 15 patients with aggressive periodontal disease (Group III). At baseline and six weeks after NSPT, crevicular fluid and clinical measures were made. The GCF IL-34, factor kB ligand, and osteoprotegrin levels were measured by using ELISA and their relative ratios were computed. Result: Group II and III had significantly higher crevicular fluid IL-34 and factor kB ligand levels than the Control group (I), although crevicular fluid with osteoprotegrin levels was significantly decreased (P<0.05). GCF, IL-34, and RANKL levels reduced in Groups II and III six weeks after NSPT, although OPG concentrations increased statistically (P<0.05). Significantly positive association was observed between IL-34 and factor kB ligand, clinical attachment level, and gingival index, but not with osteoprotegrin. Conclusion: Gingival crevicular fluid IL-34 levels were elevated in individuals with periodontal disease and lowered during non surgical intervention, and its levels correlated positively with the factor kB ligand and osteoprotegrin ratios, clinical attachment level, and gingival index. Keywords: Periodontitis, IL-34, osteoprotegrin

scholarly journals Inpatient Evaluation of Periodontal, Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth

2013 ◽  
Vol 14 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Güliz N Güncü ◽  
Gülüzar Büyüktepe ◽  
Sezen B AÕkin ◽  
Kamer Klllnç ◽  
Celal Tümer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Clinical Attachment Level ◽  
Inflammatory Parameters ◽  
Probing Depth ◽  
Natural Teeth ◽  
Fixed Prosthesis ◽  
Crevicular Fluid ◽  
Attachment Level

ABSTRACT Aim The use of endosseous dental implants (DI) has become a successful treatment alternative. However, providing periimplant tissue health and achieving a natural esthetic look are important topics in this treatment. The aim of the present study was to evaluate periodontal and esthetic parameters around DI and natural teeth (NT) and also to analyze myeloperoxidase (MPO) levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF). Materials and methods Twenty DI supported fixed prosthesis and contralateral 20 NT were enrolled to the present study. Clinical periodontal parameters (probing depth, clinical attachment level, gingival bleeding time index and gingival index) were recorded and GCF/PISF samples were obtained from mesial (mesiobuccal and mesiolingual) and distal (distobuccal and distolingual) sites of DI and NT. MPO levels were spectrophotometrically determined. Additionally clinical photographs were obtained and esthetical evaluations were performed by using Jemt papilla index. The parameters belong to DI and NT were compared and correlations were evaluated using statistical analysis. Results A total of 40 samples were evaluated. No statistically significant differences were detected between groups in all periodontal parameters and MPO levels from mesial and distal sites. Jemt papilla index scores were slightly higher in NT however, this difference was not statistically significant (p > 0.05). Total PES score were similiar in DI and NT groups. Significant correlations were detected between MPO and gingival index values as expected. Clinical significance Dental implants are satisfactory treatments, they provide patient esthetic natural looking, phonetic and masticatory functions. How to cite this article Güncü GN, Büyüktepe G, AÕkin SB, KlllnH K, Tümer C, Tözüm TF. Inpatient Evaluation of Periodontal Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth. J Contemp Dent Pract 2013;14(1):90-95.

Analysis of IL-10 in HIV-1 patients with chronic periodontitis in northern Brazil

2017 ◽  
Vol 16 ◽  
pp. 1-9
Author(s):  
Silvio Augusto Fernandes de Menezes ◽  
Tatiany Oliveira de Alencar Menezes ◽  
Tânia Maria de Souza Rodrigues ◽  
Brenna Magdalena Lima Nogueira ◽  
Ricardo Roberto de Souza Fonseca
Keyword(s):  
Viral Load ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Northern Brazil ◽  
Crevicular Fluid ◽  
Attachment Level ◽  
Hiv 1

Aim: The objective of this study was to investigate the levels of IL-10 in the gingival crevicular fluid in HIV-1 positive patients with chronic periodontitis and to compare with HIV-1 negative patients with chronic periodontitis, also to correlate clinical periodontal parameters, viral load and count of CD4+ and CD8+ lymphocytes (LTCD4+ and LTCD8+). Methods: 33 patients were selected and splitted into two groups: 16 HIV-1 positive patients and 17 HIV-1 negative patients and all with chronic periodontitis. The clinical periodontal parameters recorded were: Probing Depth (PD) and Clinical Attachment Level (CAL); the sistemical parameters LTCD4+, LTCD8+ and viral load were analized by the gingival crevicular fluid collected from all patients. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentrations of Interleukin (IL)-10. For the statistical analysis the Student t, Mann-Whitney and Spearman tests were performed. IL-10 levels were significantly lower in both patients groups. Results: There was statistical difference betwen groups for probing depth (p=0.015) and clinical attachment level (p=0.011), no significant correlation was found among the analyzed variables. Conclusion: The IL-10 levels in HIV-1 positive patients had no influence in periodontal and medical parameters.

scholarly journals Probing Pocket Depth and Clinical Attachment Level between Non-Surgical and Surgical Periodontal Therapy in Chronic Periodontitis Patients: A Randomised Controlled Clinical Trial

2018 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
Harish Kumar Shah ◽  
Shivalal Sharma ◽  
Khushboo Goel ◽  
Sajeev Shrestha ◽  
Surya Raj Niraula
Keyword(s):  
Clinical Trial ◽  
Surgical Therapy ◽  
Chronic Periodontitis ◽  
Controlled Clinical Trial ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Probing Pocket Depth ◽  
The Mean ◽  
The Difference ◽  
Attachment Level

Background: : Chronic periodontitis is one of the most common form of periodontal diseases which either require non-surgical periodontal therapy or open flap debridement-surgical therapy or both. To date, it is unclear as of how much changes occur after NSPT or OFD and which therapy provides the best outcome in chronic periodontitis having probing pocket depth ≥ 5-7 mm. Aim: The aim of this randomized controlled clinical trial was to evaluate the Probing Pocket Depth and Clinical Attachment Level between NSPT and OFD in chronic periodontitis patients. Materials and Methods: A total of 52 healthy patients with PPD ≥ 5-7 mm were included in the present study. Half of the patients assigned for the NSPT and half in the OFD group. The PPD and CAL were measured at baseline, three and six months. Independent sample t-test was used to compare the change in mean PPD and CAL between NSPT and OFD group at three and six months, respectively. Results: The difference in the mean decrease of PPD between NSPT and OFD group at three and six months were 0.15 mm (P<0.05) and 0.19 mm (P<0.05), respectively. The difference in the mean gain of CAL between NSPT and OFD group at three and six months were 0.03 mm (p>0.05) and 0.12 mm (P<0.05), respectively. Conclusion: Substantial improvement in periodontal status occurred with both the therapies, however, significantly higher decrease in PPD and gain in CAL were seen with surgical therapy.

scholarly journals Nutritional intervention in patients with periodontal disease: clinical, immunological and microbiological variables during 12 months

2008 ◽  
Vol 101 (6) ◽  
pp. 879-885 ◽  
Author(s):  
Axel Jenzsch ◽  
Sigrun Eick ◽  
Fausi Rassoul ◽  
Regina Purschwitz ◽  
Holger Jentsch
Keyword(s):  
Metabolic Syndrome ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Nutritional Intervention ◽  
Gingival Index ◽  
Granulocyte Elastase ◽  
The Metabolic Syndrome ◽  
Probing Depth ◽  
Crevicular Fluid

The role of nutrition in onset, progression and treatment of periodontitis has not been thoroughly evaluated. In the present prospective clinical study, we investigated the influence of a nutritional intervention on changes in clinical, microbiological and immunological periodontal variables during a period of 12 months in patients with the metabolic syndrome and chronic periodontitis. Twenty female subjects with the metabolic syndrome and mild to moderate chronic periodontitis participated in a guided nutritional intervention programme. Examinations were assessed before, and at 2 weeks, 3, 6 and 12 months after intervention. Clinical measurements included probing depth, Löe and Silness gingival index and Quigley–Hein plaque index. In gingival crevicular fluid, periodontopathogens, levels of IL-1β and IL-6 as well as the activity of granulocyte elastase were determined. In stimulated saliva, antioxidative and oxidative variables were measured. After 12 months the following significant changes could be observed: reduction of clinical probing depth (2·40 v. 2·20 mm; P < 0·001), reduction of gingival inflammation (gingival index 1·13 v. 0·9; P < 0·001), reduced concentrations of IL-1β (4·63 v. 1·10 pg/ml per site; P < 0·001) as well as IL-6 (1·85 v. 0·34 pg/ml per site; P = 0·022) in gingival crevicular fluid. Bacterial counts in gingival crevicular fluid as well as oxidative and antioxidative variables in saliva showed no significant changes. Only salivary catalase showed a tendency to lower values. These findings indicate that in patients with the metabolic syndrome wholesome nutrition might reduce inflammatory variables of periodontal disease and promote periodontal health.

scholarly journals Effects of statins as local drug delivery agents in treating chronic periodontitis and their antimicrobial effects using polymerase chain reaction

2020 ◽  
Vol 3 ◽  
pp. 82-88
Author(s):  
Ritunja Singh ◽  
Gangesh B. Singh ◽  
Soumya Gupta ◽  
Anil Agrawal
Keyword(s):  
Chronic Periodontitis ◽  
Periodontal Pocket ◽  
Pocket Depth ◽  
Female Ratio ◽  
Clinical Attachment Level ◽  
Antimicrobial Effects ◽  
Intergroup Comparison ◽  
Anti Inflammatory ◽  
Root Planning ◽  
Attachment Level

Objectives: The aim of the study was to evaluate the anti-inflammatory effect of statin medication in chronic periodontitis patients and to compare the change in periodontal probing depth and clinical attachment level using 1.2% atorvastatin (ATV) gel and scaling and root planning (SRP) compared to SRP alone. Materials and Methods: This study was carried out on a sample size of 40 patients with equal male and female ratio between the age group of 40–60 years having chronic periodontitis with a minimum of 20 teeth that were selected for the study. Bilateral quadrants were selected and a split mouth study was conducted. Supragingival scaling was carried out in each patient in one long appointment. The patient was then recalled after 1 week for subgingival SRP. Root planing was carried out in two consecutive visits. Left side of the mouth on the 1st day followed by right side of the mouth on the next day. On the 2nd day, after completion of the root planning, followed by placement of 1.2% ATV gel and finally the Coe Pak was placed in one quadrant which was called the test site. In the other quadrant which was called control site placebo gel was placed and the treated site was covered by the Coe Pak. The recording of clinical parameters (plaque index [PI], gingival index [GI], probing pocket depth, and clinical attachment loss) was done at baseline, 1 month and 3 months. The selected site was sampled for subgingival microflora. The data obtained were subjected to statistical analysis. One-way ANOVA, Tukey’s HSD test, and student t-test were used for intergroup and intragroup comparison. Results: In our study, when intergroup comparison of mean value for PI at baseline, 1 month and 3 months was found to clinically insignificant for control and test groups, while for GI, periodontal pocket depth, and clinical attachment level it was found insignificant at baseline while significant at 1 and 3 months. Similarly, when comparison was made for microbial count it was found clinically insignificant between control and test group at baseline, while significant was noted at 3-month interval. Conclusion: Our study evaluated the anti-inflammatory, osteoconductive and antimicrobial effects of atorvastatin giving significant reduction in PI, GI, PPD and gain in CAL along with significant decrease in the microbial load.

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