scholarly journals BMP-2 Grafted nHA/PLGA Hybrid Nanofiber Scaffold Stimulates Osteoblastic Cells Growth

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Adnan Haider ◽  
Sukyoung Kim ◽  
Man-Woo Huh ◽  
Inn-Kyu Kang
Keyword(s):  
Targeted Delivery ◽  
Bone Tissue Regeneration ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Spectroscopic Techniques ◽  
Nanofiber Scaffold ◽  
Morphogenetic Protein ◽  
Nanofiber Scaffolds ◽  
Hybrid Nanofiber

Biomaterials play a pivotal role in regenerative medicine, which aims to regenerate and replace lost/degenerated tissues or organs. Natural bone is a hierarchical structure, comprised of various cells having specific functions that are regulated by sophisticated mechanisms. However, the regulation of the normal functions in damaged or injured cells is disrupted. In order to address this problem, we attempted to artificially generate a scaffold for mimicking the characteristics of the extracellular matrix at the nanoscale level to trigger osteoblastic cell growth. For this purpose, we have chemically grafted bone morphogenetic protein (BMP-2) onto the surface of L-glutamic acid modified hydroxyapatite incorporated into the PLGA nanofiber matrix. After extensive characterization using various spectroscopic techniques, the BMP-g-nHA/PLGA hybrid nanofiber scaffolds were subjected to variousin vitrocytocompatibility tests. The results indicated that BMP-2 on BMP-g-nHA/PLGA hybrid nanofiber scaffolds greatly stimulated osteoblastic cells growth, contrary to the nHA/PLGA and pristine PLGA nanofiber scaffold, which are used as control. These results suggest that BMP-g-nHA/PLGA hybrid nanofiber scaffold can be used as a nanodrug carrier for the controlled and targeted delivery of BMP-2, which will open new possibilities for enhancing bone tissue regeneration and will help in the treatment of various bone-related diseases in the future.

Cultures of human osteoblastic cells from dialysis patients: Influence of bone turnover rate on in vitro secretion of interleukin-6 and osteoblastic cell markers

2001 ◽  
Vol 37 (1) ◽  
pp. 30-37 ◽  
Author(s):  
M. Carmen Sánchez ◽  
M. Auxiliadora Bajo ◽  
Rafael Selgas ◽  
Alberto Mate ◽  
M. Jesús Sánchez-Cabezudo ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Interleukin 6 ◽  
Turnover Rate ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Dialysis Patients ◽  
Cell Markers ◽  
Human Osteoblastic Cells

Pamidronic acid-grafted nHA/PLGA hybrid nanofiber scaffolds suppress osteoclastic cell viability and enhance osteoblastic cell activity

2016 ◽  
Vol 4 (47) ◽  
pp. 7596-7604 ◽  
Author(s):  
Adnan Haider ◽  
Davy-louis Versace ◽  
Kailash Chandra Gupta ◽  
Inn-Kyu Kang
Keyword(s):  
Bone Resorption ◽  
Cell Viability ◽  
Pamidronic Acid ◽  
Cell Activity ◽  
Osteoblastic Cell ◽  
Osteoclast Activity ◽  
Nanofiber Scaffolds ◽  
Hybrid Nanofiber ◽  
Osteoclastic Cell

Controlling osteoclast activity helps in prevention of bone resorption.

scholarly journals Factorial Study of Compressive Mechanical Properties and PrimaryIn VitroOsteoblast Response of PHBV/PLLA Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Naznin Sultana ◽  
Tareef Hayat Khan
Keyword(s):  
Mechanical Properties ◽  
Polymer Matrix ◽  
Tissue Regeneration ◽  
Degradation Rate ◽  
Bone Tissue Regeneration ◽  
Osteoblastic Cells ◽  
Composite Scaffolds ◽  
Biomimetic Systems ◽  
Human Osteoblastic Cells

For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffoldsin vitrowere also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds.In vitrostudies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds.

scholarly journals ROS-Mediated Necroptosis Is Involved in Iron Overload-Induced Osteoblastic Cell Death

2020 ◽  
Vol 2020 ◽  
pp. 1-22 ◽  
Author(s):  
Qing Tian ◽  
Bo Qin ◽  
Yufan Gu ◽  
Lijun Zhou ◽  
Songfeng Chen ◽  
...  
Keyword(s):  
Cell Death ◽  
Iron Overload ◽  
Mitochondrial Permeability Transition ◽  
Cell Damage ◽  
Critical Role ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Ros Generation ◽  
Mptp Opening

Excess iron has been reported to lead to osteoblastic cell damage, which is a crucial pathogenesis of iron overload-related osteoporosis. However, the cytotoxic mechanisms have not been fully documented. In the present study, we focused on whether necroptosis contributes to iron overload-induced osteoblastic cell death and related underlying mechanisms. Here, we showed that the cytotoxicity of iron overload in osteoblastic cells was mainly due to necrosis, as evidenced by the Hoechst 33258/PI staining, Annexin-V/PI staining, and transmission electronic microscopy. Furthermore, we revealed that iron overload-induced osteoblastic necrosis might be mediated via the RIPK1/RIPK3/MLKL necroptotic pathway. In addition, we also found that iron overload was able to trigger mitochondrial permeability transition pore (mPTP) opening, which is a critical downstream event in the execution of necroptosis. The key finding of our experiment was that iron overload-induced necroptotic cell death might depend on reactive oxygen species (ROS) generation, as N-acetylcysteine effectively rescued mPTP opening and necroptotic cell death. ROS induced by iron overload promote necroptosis via a positive feedback mechanism, as on the one hand N-acetylcysteine attenuates the upregulation of RIPK1 and RIPK3 and phosphorylation of RIPK1, RIPK3, and MLKL and on the other hand Nec-1, siRIPK1, or siRIPK3 reduced ROS generation. In summary, iron overload induced necroptosis of osteoblastic cells in vitro, which is mediated, at least in part, through the RIPK1/RIPK3/MLKL pathway. We also highlight the critical role of ROS in the regulation of iron overload-induced necroptosis in osteoblastic cells.

scholarly journals Morphological Effects of HA on the Cell Compatibility of Electrospun HA/PLGA Composite Nanofiber Scaffolds

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Adnan Haider ◽  
Kailash Chandra Gupta ◽  
Inn-Kyu Kang
Keyword(s):  
Tissue Engineering ◽  
Xrd Analysis ◽  
Cellular Adhesion ◽  
Bone Tissue Regeneration ◽  
Precipitation Method ◽  
Electrospinning Technique ◽  
Cell Compatibility ◽  
Composite Nanofiber ◽  
Nanofiber Scaffolds

Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results fromin vitrostudies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca+2ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.

scholarly journals In vitro evaluation of the effects of risedronate associated with cobalamin on osteoblastic cells

2015 ◽  
Vol 63 (2) ◽  
pp. 161-168
Author(s):  
Jaqueline Aparecida FIUZA ◽  
Elizabeth Ferreira MARTINEZ ◽  
Rui Barbosa de BRITO JÚNIOR
Keyword(s):  
Cell Proliferation ◽  
Ascorbic Acid ◽  
Cell Cultures ◽  
Growth Curve ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
In Vitro Evaluation ◽  
The Media

OBJECTIVE: To evaluate the action of risedronate and cobalamin, and effects when associated, when administered osteoblastic cells. METHODS: The MC3T3 cells were cultivate in the media α-MEM and α-MEM supplemented with mineralizing factors, ascorbic acid and disodium α-glyicerophosphate, and treated with risedronate, cobalamin, and risedronate associated with cobalamin in a concentration of 10-3 M. The cell proliferation and formation of calcium and phosphate nodules were evaluated at 24 hours, 48 hours, 72 hours, 5 days and 7 days via a Neubauer Chamer count, Alizarin and the von Kossa reaction. RESULTS: The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. CONCLUSION: The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules.

scholarly journals Differentiating Co-Delivery of Bisphosphonate and Simvastatin by Self-Healing Hyaluronan Hydrogel Formed by Orthogonal “Clicks”: An In-Vitro Assessment

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2106
Author(s):  
Dmitri A. Ossipov ◽  
Mads Lüchow ◽  
Michael Malkoch
Keyword(s):  
Targeted Delivery ◽  
Release Kinetics ◽  
Bone Diseases ◽  
Bone Tissue Regeneration ◽  
Potential Candidate ◽  
Self Healing ◽  
Network Properties ◽  
Multiple Drugs ◽  
Hydrophobic Ligands

Due to its unique properties resembling living tissues, hydrogels are attractive carriers for the localized and targeted delivery of various drugs. Drug release kinetics from hydrogels are commonly controlled by network properties and the drug-network interactions. However, and simultaneously, the programmable delivery of multiple drugs with opposing properties (hydrophilicity, molecular weight, etc.) from hydrogels with determined network properties is still challenging. Herein, we describe the preparation of injectable self-healing hyaluronic acid (HA) hydrogels that release hydrophobic simvastatin and hydrophilic aminobisphosphonate (BP) drugs independently in response to acidic and thiol-containing microenvironments, respectively. We apply a prodrug strategy to BP by conjugating it to HA via a self-immolative disulfide linker that is stable in the blood plasma and is cleavable in the cytoplasm. Moreover, we utilize HA-linked BP ligands to reversibly bind Ca2+ ions and form coordination hydrogels. Hydrazone coupling of hydrophobic ligands to HA permits the encapsulation of simvastatin molecules in the resulting amphiphilic HA derivative and the subsequent acid-triggered release of the drug. The conjugation of BP and hydrophobic ligands to HA enables preparation of both bulk self-healing hydrogels and nanogels. Moreover, the developed hydrogel system is shown to be multi-responsive by applying orthogonally cleavable linkers. The presented hydrogel is a potential candidate for the combination treatment of osteoporosis and bone cancers as well as for bone tissue regeneration since it can deliver bone anabolic and anti-catabolic agents in response to bone diseases microenvironments.

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