scholarly journals Factorial Study of Compressive Mechanical Properties and PrimaryIn VitroOsteoblast Response of PHBV/PLLA Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Naznin Sultana ◽  
Tareef Hayat Khan
Keyword(s):  
Mechanical Properties ◽  
Polymer Matrix ◽  
Tissue Regeneration ◽  
Degradation Rate ◽  
Bone Tissue Regeneration ◽  
Osteoblastic Cells ◽  
Composite Scaffolds ◽  
Biomimetic Systems ◽  
Human Osteoblastic Cells

For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffoldsin vitrowere also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds.In vitrostudies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds.

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

Strategies to Improve Bioactivity of Hydroxyapatite Bone Scaffolds

2017 ◽  
Vol 758 ◽  
pp. 132-137 ◽  
Author(s):  
Francesca Scalera ◽  
Francesca Gervaso ◽  
Barbara Palazzo ◽  
Stefania Scialla ◽  
Daniela Izzo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Weight Loss ◽  
Tissue Regeneration ◽  
Structural Modification ◽  
Physiological Solution ◽  
Bone Tissue Regeneration ◽  
In Vitro Tests ◽  
Bone Scaffolds ◽  
Good Biocompatibility

Two different approaches are proposed in this study to enhance the bioactivity of hydroxyapatite-based scaffolds for bone tissue regeneration. The first method consists in a structural modification of Hydroxyapatite (HA) through doping it with Magnesium (1,3% wt) while the second one in using HA in combination with a calcium silicate, i.e. Wollastonite (WS), to form a composite bioceramic. Scaffolds with high and strongly interconnected porosity (pores ranging from 300 to 800 µm) were produced throughout both procedures. Higher mechanical properties in compression were obtained when the composite Ws/HA bioceramic was adopted. That one showed a weight loss after 6 months in physiological solution seven times higher than doped HA. Preliminary in vitro tests highlighted that both kinds of scaffold allowed the adhesion of MG63, without significant differences in terms of vitality, indicating a good biocompatibility of both used biomaterials.

scholarly journals Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

2021 ◽  
Vol 22 (13) ◽  
pp. 6794
Author(s):  
Jae-Woo Kim ◽  
Yoon-Soo Han ◽  
Hyun-Mee Lee ◽  
Jin-Kyung Kim ◽  
Young-Jin Kim
Keyword(s):  
Hyaluronic Acid ◽  
Bone Tissue ◽  
Double Layer ◽  
Tissue Regeneration ◽  
Great Influence ◽  
Morphological Characteristics ◽  
Bone Tissue Regeneration ◽  
Hierarchical Architecture ◽  
Uniform Structure ◽  
Composite Scaffolds

The use of porous three-dimensional (3D) composite scaffolds has attracted great attention in bone tissue engineering applications because they closely simulate the major features of the natural extracellular matrix (ECM) of bone. This study aimed to prepare biomimetic composite scaffolds via a simple 3D printing of gelatin/hyaluronic acid (HA)/hydroxyapatite (HAp) and subsequent biomineralization for improved bone tissue regeneration. The resulting scaffolds exhibited uniform structure and homogeneous pore distribution. In addition, the microstructures of the composite scaffolds showed an ECM-mimetic structure with a wrinkled internal surface and a porous hierarchical architecture. The results of bioactivity assays proved that the morphological characteristics and biomineralization of the composite scaffolds influenced cell proliferation and osteogenic differentiation. In particular, the biomineralized gelatin/HA/HAp composite scaffolds with double-layer staggered orthogonal (GEHA20-ZZS) and double-layer alternative structure (GEHA20-45S) showed higher bioactivity than other scaffolds. According to these results, biomineralization has a great influence on the biological activity of cells. Hence, the biomineralized composite scaffolds can be used as new bone scaffolds in bone regeneration.

Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration

2021 ◽  
Vol 122 ◽  
pp. 111928
Author(s):  
Eduardo H. Backes ◽  
Emanuel M. Fernandes ◽  
Gabriela S. Diogo ◽  
Catarina F. Marques ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Phosphates ◽  
Bone Tissue Regeneration ◽  
Aliphatic Polyester ◽  
Composite Scaffolds ◽  
3D Printed

In Vitro and In Vivo Degradation, Mechanical Properties, and Histocompatibility of Weft-Knitted Silk Mesh-Like Grafts

2022 ◽  
Vol 12 (2) ◽  
pp. 411-416
Author(s):  
Liang Tang ◽  
Si-Yu Zhao ◽  
Ya-Dong Yang ◽  
Geng Yang ◽  
Wen-Yuan Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Degradation Rate ◽  
Maximum Load ◽  
Artificial Ligament ◽  
In Vivo Degradation

To investigate the degradation, mechanical properties, and histocompatibility of weft-knitted silk mesh-like grafts, we carried out the In Vitro and In Vivo silk grafts degradation assay. The In Vitro degradation experiment was performed by immersing the silk grafts in simulated body fluid for 1 year, and the results showed that the degradation rate of the silk mesh-like grafts was very slow, and there were few changes in the mechanical properties and quality of the silk mesh-like graft. In Vivo degradation assay was taken by implantation of the silk mesh-like grafts into the subcutaneous muscles of rabbits. At 3, 6, and 12 months postoperation, the rate of mass loss was 19.36%, 31.84%, and 58.77%, respectively, and the maximum load was 63.85%, 34.63%, and 10.76%, respectively of that prior to degradation. The results showed that the degradation rate of the silk graft and the loss of mechanical properties In Vivo were faster than the results obtained in the In Vitro experiments. In addition, there were no significant differences in secretion of serum IL-6 and TNF-α between the experimental and normal rabbits (P >0.05), suggesting no obvious inflammatory reaction. The findings suggest that the weft-knitted silk mesh-like grafts have good mechanical properties, histocompatibility, and In Vivo degradation rate, and therefore represent a candidate material for artificial ligament

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