scholarly journals Inhibitory Effect of Sihuangxiechai Decoction on Ovalbumin-Induced Airway Inflammation in Guinea Pigs

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Xue Ping Huang ◽  
En Xue Tao ◽  
Zhan Qin Feng ◽  
Zhao Lu Yang ◽  
Wei Fen Zhang
Keyword(s):  
Guinea Pig ◽  
Airway Inflammation ◽  
Treated Group ◽  
Pig Model ◽  
Interleukin 4 ◽  
Necrosis Factor Alpha ◽  
Cell Counts ◽  
Significant Difference ◽  
Guinea Pig Model ◽  
Asthma Group

The aim of this study was to investigate the effect of sihuangxiechai decoction on asthmatic Guinea pig model which was sensitized by intraperitoneal (i.p.) injection of ovalbumin (OVA) and challenged by OVA inhalation to induce chronic airway inflammation. Differential cell counts of cytospins were performed after staining with Giemsa solution. The quantity of leukocytes and its classification in bronchoalveolar lavage fluid (BALF) and blood were evaluated by blood cell analyzer and microscope. Histological analysis of the lung was performed by hematoxylin and eosin (H&E) staining. The levels of interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-α) in BALF and serum were detected by radioimmunoassay (RIA). The total number of leukocytes in BALF and blood has no significant difference between Sihuangxiechaitang decoction treated group and dexamethasone (DXM) treated group but was significantly lower than those of asthma group. The percentage of eosinophils in lung tissues of sihuangxiechai decoction treated group was significantly lower than that of asthma group. The results demonstrated that the levels of IL-4 and TNF-αin the sihuangxiechai decoction treated group were significantly reduced compared with the asthma group. In conclusion, these findings demonstrate that sihuangxiechai decoction has a protective effect on OVA-induced asthma in reducing airway inflammation and airway hyperresponsiveness (AHR) in a Guinea pig model and may be useful as an adjuvant therapy for the treatment of bronchial asthma.

scholarly journals Ceramide: a Key Signaling Molecule in a Guinea Pig Model of Allergic Asthmatic Response and Airway Inflammation

2007 ◽  
Vol 324 (2) ◽  
pp. 548-557 ◽  
Author(s):  
Emanuela Masini ◽  
Lucia Giannini ◽  
Silvia Nistri ◽  
Lorenzo Cinci ◽  
Rosanna Mastroianni ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Airway Inflammation ◽  
Pig Model ◽  
Signaling Molecule ◽  
Asthmatic Response ◽  
Allergic Asthmatic ◽  
Guinea Pig Model

scholarly journals Three Protein Cocktails Mediate Delayed-Type Hypersensitivity Responses Indistinguishable from That Elicited by Purified Protein Derivative in the Guinea Pig Model ofMycobacterium tuberculosisInfection

2010 ◽  
Vol 79 (2) ◽  
pp. 716-723 ◽  
Author(s):  
Hongliang Yang ◽  
JoLynn Troudt ◽  
Ajay Grover ◽  
Kimberly Arnett ◽  
Megan Lucas ◽  
...  
Keyword(s):  
T Cells ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Ex Vivo ◽  
Interleukin 10 ◽  
Pig Model ◽  
Delayed Type Hypersensitivity ◽  
Necrosis Factor Alpha ◽  
Purified Protein Derivative ◽  
Guinea Pig Model

ABSTRACTPurified protein derivative (PPD) is a widely used reagent for the diagnosis ofMycobacterium tuberculosisinfection. Recently, the molecular composition of PPD was defined, with hundreds of mycobacterial protein representatives making up PPD. Which, if any, of these specific products drive the potency of PPD remains in question. In this study, two proteins (DnaK and GroEL2) previously identified as dominant proteins in PPD were tested for the capacity to induce delayed-type hypersensitivity (DTH) responses in H37Rv-infected or BCG-vaccinated guinea pigs. These two proteins were used in pull-down assays to identify interacting PPD products. Six proteins were identified as interacting partners with DnaK and GroEL2, i.e., Rv0009, Rv0475, Rv0569, Rv0685, Rv2626c, and Rv2632c. These six proteins were tested alone and in combination with DnaK and GroEL2 for the capacity to induce a DTH response in the guinea pig model. From these studies, two cocktails, DnaK/GroEL2/Rv0009 and DnaK/GroEL2/Rv0685, were found to induce DTH responses in H37Rv-infected or BCG-vaccinated guinea pigs that were indistinguishable from DTH responses driven by a PPD injection. The mechanism by which DTH responses were induced was elucidated by histologic examination, analysis of activated CD4+/CD8+T cells, and cytokine mRNA expression at the site of the DTH response. PPD and the protein cocktails tested induced strong DTH responses in H37Rv-infected guinea pigs. Ex vivo phenotyping of T cells at the DTH site indicated that this response is mediated by activated CD4+and CD8+T cells, with increases in gamma interferon and tumor necrosis factor alpha, but not interleukin-10, at the site of the DTH response. Our results demonstrate for the first time that the PPD response can be mimicked at the molecular level with defined protein cocktails. The use of this defined product will allow a more thorough understanding of the DTH response and may provide a platform for more rapid and sensitive second-generation skin test reagents for the diagnosis ofM. tuberculosisinfection.

Regulation of Neurogenic Airway Inflammation via the Dorsal Vagal Complex in a Guinea Pig Model of Gastroesophageal Reflux

CHEST Journal ◽  
2016 ◽  
Vol 149 (4) ◽  
pp. A26
Author(s):  
Zhe Chen ◽  
Le-jia Sun ◽  
Hui Chen ◽  
Da-chuan Gu ◽  
Wei-tao Zhang ◽  
...  
Keyword(s):  
Gastroesophageal Reflux ◽  
Guinea Pig ◽  
Airway Inflammation ◽  
Pig Model ◽  
Dorsal Vagal Complex ◽  
Guinea Pig Model

scholarly journals Combination therapy of tiotropium and ciclesonide attenuates airway inflammation and remodeling in a guinea pig model of chronic asthma

2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Loes E. M. Kistemaker ◽  
I. Sophie T. Bos ◽  
Mark H. Menzen ◽  
Harm Maarsingh ◽  
Herman Meurs ◽  
...  
Keyword(s):  
Combination Therapy ◽  
Guinea Pig ◽  
Airway Inflammation ◽  
Pig Model ◽  
Chronic Asthma ◽  
Guinea Pig Model

Aerosolized Polymerized Type I Collagen Reduces Airway Inflammation and Remodelling in a Guinea Pig Model of Allergic Asthma

Lung ◽  
2009 ◽  
Vol 188 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Paola Moreno-Alvarez ◽  
Edgar Sánchez-Guerrero ◽  
Erasmo Martínez-Cordero ◽  
Rogelio Hernández-Pando ◽  
María G. Campos ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Airway Inflammation ◽  
Allergic Asthma ◽  
Type I Collagen ◽  
Pig Model ◽  
Type I ◽  
Guinea Pig Model
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