scholarly journals Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ling Zhang ◽  
Xiang-dong Yang ◽  
Yuan-yu Zhang ◽  
Jing Yang ◽  
Guang-xun Qi ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Antisense Rna ◽  
Seed Oil ◽  
Soybean Seed ◽  
Cotyledonary Node ◽  
Fatty Acid Analysis ◽  
Oleic Acid Content ◽  
Oleate Desaturase ◽  
Key Enzyme

The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturaseGmFad2-1bgene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybeanGmFad2-1b801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoterBCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression ofGmFad2-1bin the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.

Heritability of Oleic Acid Content in Soybean Seed Oil and Its Genetic Correlation with Fatty Acid and Agronomic Traits

Crop Science ◽  
2008 ◽  
Vol 48 (5) ◽  
pp. 1764-1772 ◽  
Author(s):  
Eleni Bachlava ◽  
Joseph W. Burton ◽  
Cavell Brownie ◽  
Sanbao Wang ◽  
Jérôme Auclair ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Genetic Correlation ◽  
Acid Content ◽  
Seed Oil ◽  
Agronomic Traits ◽  
Soybean Seed ◽  
Oleic Acid Content

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

scholarly journals INFLUENCE OF DRYING AIR VOLUMES ON FATTY ACID COMPOSITION OF PECANS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1140b-1140
Author(s):  
Robert Brown ◽  
Laurence Sistrunk ◽  
William Aldred ◽  
J. Benton Storey
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Room Temperature ◽  
Packed Column ◽  
Frozen Storage ◽  
Fatty Acid Analysis ◽  
Oleic Acid Content ◽  
Air Volume ◽  
Random Samples

`Stuart' pecans were harvested as soon as shucks would split in the fall of 1989 and 45 kg inshell samples were placed in 30 × 30 × 105 cm drying bins. The nuts were dried at air volumes of either 0, 1.27, 1.56, 1.84, or 2.12 m3/min down to 4% moisture. Air temperature in the drying bins was maintained at uniform 35°C with the exception of the 0 air volume treatment which was allowed to dry at room temperature. Four random samples of each treatment were held in frozen storage awaiting fatty acid analysis. Palmitic, stearic, oleic, linoleic, and linolinic fatty acids were separated in a 183 cm × 3 mm packed column using a 10% Silar 10C phase on a Gas Chrom QII, 100/120. The samples dried with a air volume of 1.27 m3/min retained a significantly higher oleic acid content than the 0 and 2.12 m3/min drying volumes. The 1.27 m3/min volume retained 64.55 % oleic acid compared with 61.37'% for the 0 velocity sample and 59.61% for 2.12 m3/min treatment. The more desirable oleic/linoleic ratio of 2.24 was found in the 1.27 m3/min sample compared to a 1.78 ratio in the 2.12 m3/min sample. Increased volume of air in the drying bins was thus deleterious to these samples because of the loss of monounsaturated fatty acid.

scholarly journals Heritability of oleic acid content in sunflower seed oil of recombinant inbred lines

2020 ◽  
Vol 183 (3) ◽  
pp. 27-30
Author(s):  
Ya.N. Demurin ◽  
◽  
Yu.V. Chebanova ◽  
O.M. Borisenko ◽  
T.A. Kovalenko ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Recombinant Inbred ◽  
Acid Content ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Oleic Acid Content

The study of the heritability of the oleic acid content in seed oil in recombinant inbred lines is the genetic basis for effective breeding work on the quality of sunflower oil. The experiments were carried out under field and laboratory conditions in VNIIMK, Krasnodar, Russian Federation in 2016-2020. We used 17 recombinant inbred sunflower lines of I4 and I5 generations obtained from crossing a medium-oleic LG27 line and a high-oleic LG26 line with subsequent self-pollination. The fatty acid composition of sunflower seed oil was analyzed using the method of gas-liquid chromatography of methyl esters on the Chromatek-Kristall 5000 device. Seventeen recombinant inbred sunflower lines in generation I4 showed a wide variation in the content of oleic acid in the oil of average seed samples from 39.00 % (RIL-1) to 92.24 % (RIL-42) and linoleic acid – from 43.28 to 1.35 %, respectively. In 2016, three lines were characterized 28 by an average oleic acid content of 55.64-65.54 %. Analysis of the fatty acid composition of oil in individual seeds of the next generation I5 of these lines confirmed, in general, their phenotypic ranks with a range of variability from 32.18 to 92.15 % in the content of oleic acid. The middle oleic lines RIL-21, RIL29 and RIL-30 also showed belonging to their phenotypic class in 2019 in the range of values from 59.80 to 63.14 %. The study of the conjugate variability of oleic acid values in the parent-progeny series in generations of I4–I5 revealed the presence of a significant strong positive correlation r = 0.97. At the same time, the coefficient of determination, defined as the square of the correlation coefficient which evaluates the degree of heritability of a trait, was 0.95, which indicates a significant influence of the genotype factor in general phenotypic variation.

scholarly journals CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Brice A. Jarvis ◽  
Trevor B. Romsdahl ◽  
Michaela G. McGinn ◽  
Tara J. Nazarenus ◽  
Edgar B. Cahoon ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Oleic Acid Content ◽  
Wild Type ◽  
High Oleic Acid ◽  
High Oleic ◽  
Thlaspi Arvense ◽  
Trade Offs

Pennycress (Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the off-season throughout temperate regions of the world. With its diploid genome and ease of directed mutagenesis using molecular approaches, pennycress seed oil composition can be rapidly tailored for a plethora of food, feed, oleochemical and fuel uses. Here, we utilized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations in the FATTY ACID DESATURASE2 (FAD2) and REDUCED OLEATE DESATURATION1 (ROD1) genes to increase oleic acid content. High oleic acid (18:1) oil is valued for its oxidative stability that is superior to the polyunsaturated fatty acids (PUFAs) linoleic (18:2) and linolenic (18:3), and better cold flow properties than the very long chain fatty acid (VLCFA) erucic (22:1). When combined with a FATTY ACID ELONGATION1 (fae1) knockout mutation, fad2 fae1 and rod1 fae1 double mutants produced ∼90% and ∼60% oleic acid in seed oil, respectively, with PUFAs in fad2 fae1 as well as fad2 single mutants reduced to less than 5%. MALDI-MS spatial imaging analyses of phosphatidylcholine (PC) and triacylglycerol (TAG) molecular species in wild-type pennycress embryo sections from mature seeds revealed that erucic acid is highly enriched in cotyledons which serve as storage organs, suggestive of a role in providing energy for the germinating seedling. In contrast, PUFA-containing TAGs are enriched in the embryonic axis, which may be utilized for cellular membrane expansion during seed germination and seedling emergence. Under standard growth chamber conditions, rod1 fae1 plants grew like wild type whereas fad2 single and fad2 fae1 double mutant plants exhibited delayed growth and overall reduced heights and seed yields, suggesting that reducing PUFAs below a threshold in pennycress had negative physiological effects. Taken together, our results suggest that combinatorial knockout of ROD1 and FAE1 may be a viable route to commercially increase oleic acid content in pennycress seed oil whereas mutations in FAD2 will likely require at least partial function to avoid fitness trade-offs.

Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

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