scholarly journals Down-regulation of crambe fatty acid desaturase and elongase in Arabidopsis and crambe resulted in significantly increased oleic acid content in seed oil

2015 ◽  
Vol 14 (1) ◽  
pp. 323-331 ◽  
Author(s):  
Xueyuan Li ◽  
Desheng Mei ◽  
Qing Liu ◽  
Jing Fan ◽  
Surinder Singh ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Down Regulation

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

scholarly journals Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene

2020 ◽  
Author(s):  
Nan Wu ◽  
Long Jiang ◽  
Xiaoming Yu ◽  
Xiangbo Yang ◽  
Dianyuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Grain Development ◽  
Expression Levels ◽  
Soybean Fatty Acid ◽  
Structural Differences

Abstract Background To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.Results Database analysis, phylogenetic tree analysis, transmembrane structure prediction, amino acid sequence alignment, intron exon structure analysis, and protein motif analysis were performed for seven genes in the FAD2 family. The results showed that GmFAD2-1 and GmFAD2-2b and Other genes are structurally different, and it is speculated that their functions also have weak changes. By analyzing gene expression patterns at different stages of soybean grain development, the expression levels of the family genes at different stages of grain development first increase, then decrease, and finally decrease. For stability, the correlation analysis showed that the gene expression was negatively correlated with oleic acid content, and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.Conclusions Bioinformatics and expression analysis of genes in the FAD2 family. Identify the structural differences of each gene, and the differences in expression levels at different stages of soybean variety and grain development, and initially identify the differences in function size and catalytic efficiency between genes, in order to create new high-oleic soybean varieties using genetic engineering Quality, providing a theoretical basis.

scholarly journals Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene

2020 ◽  
Author(s):  
Nan Wu ◽  
Long Jiang ◽  
Xiaoming Yu ◽  
Xiangbo Yang ◽  
Dianyuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Grain Development ◽  
Expression Levels ◽  
Soybean Fatty Acid ◽  
Structural Differences

Abstract Background To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.Results Database analysis, phylogenetic tree analysis, transmembrane structure prediction, amino acid sequence alignment, intron exon structure analysis, and protein motif analysis were performed for seven genes in the FAD2 family. The results showed that GmFAD2-1 and GmFAD2-2b and Other genes are structurally different, and it is speculated that their functions also have weak changes. By analyzing gene expression patterns at different stages of soybean grain development, the expression levels of the family genes at different stages of grain development first increase, then decrease, and finally decrease. For stability, the correlation analysis showed that the gene expression was negatively correlated with oleic acid content, and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.Conclusions Bioinformatics and expression analysis of genes in the FAD2 family. Identify the structural differences of each gene, and the differences in expression levels at different stages of soybean variety and grain development, and initially identify the differences in function size and catalytic efficiency between genes, in order to create new high-oleic soybean varieties using genetic engineering Quality, providing a theoretical basis.

Enhanced Oleic Acid Content in the Soybean Mutant M23 Is Associated with the Deletion in the Fad2-1a Gene Encoding a Fatty Acid Desaturase

2007 ◽  
Vol 84 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Devinder Sandhu ◽  
Jessie L. Alt ◽  
Curtis W. Scherder ◽  
Walter R. Fehr ◽  
Madan K. Bhattacharyya
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Gene Encoding

scholarly journals Heritability of oleic acid content in sunflower seed oil of recombinant inbred lines

2020 ◽  
Vol 183 (3) ◽  
pp. 27-30
Author(s):  
Ya.N. Demurin ◽  
◽  
Yu.V. Chebanova ◽  
O.M. Borisenko ◽  
T.A. Kovalenko ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Recombinant Inbred ◽  
Acid Content ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Oleic Acid Content

The study of the heritability of the oleic acid content in seed oil in recombinant inbred lines is the genetic basis for effective breeding work on the quality of sunflower oil. The experiments were carried out under field and laboratory conditions in VNIIMK, Krasnodar, Russian Federation in 2016-2020. We used 17 recombinant inbred sunflower lines of I4 and I5 generations obtained from crossing a medium-oleic LG27 line and a high-oleic LG26 line with subsequent self-pollination. The fatty acid composition of sunflower seed oil was analyzed using the method of gas-liquid chromatography of methyl esters on the Chromatek-Kristall 5000 device. Seventeen recombinant inbred sunflower lines in generation I4 showed a wide variation in the content of oleic acid in the oil of average seed samples from 39.00 % (RIL-1) to 92.24 % (RIL-42) and linoleic acid – from 43.28 to 1.35 %, respectively. In 2016, three lines were characterized 28 by an average oleic acid content of 55.64-65.54 %. Analysis of the fatty acid composition of oil in individual seeds of the next generation I5 of these lines confirmed, in general, their phenotypic ranks with a range of variability from 32.18 to 92.15 % in the content of oleic acid. The middle oleic lines RIL-21, RIL29 and RIL-30 also showed belonging to their phenotypic class in 2019 in the range of values from 59.80 to 63.14 %. The study of the conjugate variability of oleic acid values in the parent-progeny series in generations of I4–I5 revealed the presence of a significant strong positive correlation r = 0.97. At the same time, the coefficient of determination, defined as the square of the correlation coefficient which evaluates the degree of heritability of a trait, was 0.95, which indicates a significant influence of the genotype factor in general phenotypic variation.

Heritability of Oleic Acid Content in Soybean Seed Oil and Its Genetic Correlation with Fatty Acid and Agronomic Traits

Crop Science ◽  
2008 ◽  
Vol 48 (5) ◽  
pp. 1764-1772 ◽  
Author(s):  
Eleni Bachlava ◽  
Joseph W. Burton ◽  
Cavell Brownie ◽  
Sanbao Wang ◽  
Jérôme Auclair ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Genetic Correlation ◽  
Acid Content ◽  
Seed Oil ◽  
Agronomic Traits ◽  
Soybean Seed ◽  
Oleic Acid Content

scholarly journals Construction and Analysis of GmFAD2-1A and GmFAD2-2A Soybean Fatty Acid Desaturase Mutants Based on CRISPR/Cas9 Technology

2020 ◽  
Vol 21 (3) ◽  
pp. 1104 ◽  
Author(s):  
Nan Wu ◽  
Qiang Lu ◽  
Piwu Wang ◽  
Qi Zhang ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Protein Content ◽  
Acid Content ◽  
Double Mutant ◽  
Fatty Acid Desaturase ◽  
Linoleic Acid Content ◽  
Oleic Acid Content ◽  
Editing Efficiency

The soybean fatty acid desaturase family is composed of seven genes, but the function of each gene has not been reported. Bioinformatics was used to analyse the structure of genes in this family, as well as the correlation between Δ12-fatty acid desaturase II (FAD2) expression and oleic acid content on different days after flowering of soybean. In the present study, CRISPR/Cas9 technology was used to construct single and double mutant knockout vectors of functional genes in the FAD2 family. Analysis of the molecular biology and expression patterns of genes in the FAD2 family, namely, GmFAD2-1A (Glyma.10G278000) and GmFAD2-2A (Glyma.19G147300), showed that they had little homology with other soybean FAD2 genes, and that their function was slightly changed. Sequencing of the target showed that the editing efficiency of the GmFAD2-1A and GmFAD2-2A genes was 95% and 55.56%, respectively, and that the double mutant editing efficiency was 66.67%. The mutations were divided into two main types, as follows: base deletion and insertion. A near-infrared grain analyser determined the following results: In the T2 generation, the oleic acid content increased from 17.10% to 73.50%; the linoleic acid content decreased from 62.91% to 12.23%; the protein content increased from 37.69% to 41.16%; in the T3 generation, the oleic acid content increased from 19.15% to 72.02%; the linoleic acid content decreased from 56.58% to 17.27%. In addition, the protein content increased from 37.52% to 40.58% compared to that of the JN38 control variety.

scholarly journals CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Brice A. Jarvis ◽  
Trevor B. Romsdahl ◽  
Michaela G. McGinn ◽  
Tara J. Nazarenus ◽  
Edgar B. Cahoon ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Oleic Acid Content ◽  
Wild Type ◽  
High Oleic Acid ◽  
High Oleic ◽  
Thlaspi Arvense ◽  
Trade Offs

Pennycress (Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the off-season throughout temperate regions of the world. With its diploid genome and ease of directed mutagenesis using molecular approaches, pennycress seed oil composition can be rapidly tailored for a plethora of food, feed, oleochemical and fuel uses. Here, we utilized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations in the FATTY ACID DESATURASE2 (FAD2) and REDUCED OLEATE DESATURATION1 (ROD1) genes to increase oleic acid content. High oleic acid (18:1) oil is valued for its oxidative stability that is superior to the polyunsaturated fatty acids (PUFAs) linoleic (18:2) and linolenic (18:3), and better cold flow properties than the very long chain fatty acid (VLCFA) erucic (22:1). When combined with a FATTY ACID ELONGATION1 (fae1) knockout mutation, fad2 fae1 and rod1 fae1 double mutants produced ∼90% and ∼60% oleic acid in seed oil, respectively, with PUFAs in fad2 fae1 as well as fad2 single mutants reduced to less than 5%. MALDI-MS spatial imaging analyses of phosphatidylcholine (PC) and triacylglycerol (TAG) molecular species in wild-type pennycress embryo sections from mature seeds revealed that erucic acid is highly enriched in cotyledons which serve as storage organs, suggestive of a role in providing energy for the germinating seedling. In contrast, PUFA-containing TAGs are enriched in the embryonic axis, which may be utilized for cellular membrane expansion during seed germination and seedling emergence. Under standard growth chamber conditions, rod1 fae1 plants grew like wild type whereas fad2 single and fad2 fae1 double mutant plants exhibited delayed growth and overall reduced heights and seed yields, suggesting that reducing PUFAs below a threshold in pennycress had negative physiological effects. Taken together, our results suggest that combinatorial knockout of ROD1 and FAE1 may be a viable route to commercially increase oleic acid content in pennycress seed oil whereas mutations in FAD2 will likely require at least partial function to avoid fitness trade-offs.

Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

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