scholarly journals Three-Dimensional Aspects of the Lingual Papillae and Their Connective Tissue Cores in the Tongue of Rats: A Scanning Electron Microscope Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Gabriela de Souza Reginato ◽  
Cristina de Sousa Bolina ◽  
Ii-sei Watanabe ◽  
Adriano Polican Ciena
Keyword(s):  
Connective Tissue ◽  
Three Dimensional ◽  
Morphological Characteristics ◽  
Dorsal Surface ◽  
Sprague Dawley ◽  
Sem Images ◽  
Scanning Electron Microscope Study ◽  
Lingual Papillae ◽  
Sprague Dawley Rats ◽  
Filiform Papillae

The aim of the present study was to describe the tridimensional morphological characteristics of the lingual papillae and their connective tissue cores (CTCs) in Sprague Dawley rats. Four types of papillae were reported on the dorsal surface. Filiform papillae were distributed on the tongue surface and after epithelial maceration a conic and multifilamentary shape of the CTCs was revealed. Fungiform papillae were reported on the rostral and middle regions covered by a squamous epithelium. After the removal of the epithelium, the shape of a volcano with the taste orifice at its top was noted. Foliate papillae were composed of five pairs of epithelial folds situated on the lateral-caudal margin of the tongue. After the removal of the epithelium, they were shown to be limited by thin laminar projections. The vallate papilla with an oval shape was present in the caudal region and delimited by an incomplete groove. The morphological characteristics of the lingual papillae of Sprague Dowley rats, three-dimensional SEM images, and the types of papillae on the dorsal surface were similar to those reported previously in other rodent mammals. The maceration technique revealed the details of extracellular matrix with varied shapes form of connective tissue cores.

scholarly journals Microstructure of the Surface of the Tongue and Histochemical Study of the Lingual Glands of the Lowland Tapir (Tapirus terrestris Linnaeus, 1758) (Perissodactyla: Tapiridae)

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2297
Author(s):  
Karolina Goździewska-Harłajczuk ◽  
Pavla Hamouzová ◽  
Joanna Klećkowska-Nawrot ◽  
Karolina Barszcz ◽  
Petr Čížek
Keyword(s):  
Histochemical Study ◽  
Microscopic Analysis ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Mucous Secretion ◽  
Histochemical Analysis ◽  
Lingual Papillae ◽  
Tapirus Terrestris ◽  
Vallate Papillae ◽  
Filiform Papillae

Although the anatomy of the gastrointestinal tract has been characterized in the lowland tapir (Tapirus terrestris), the exact anatomy of its tongue has not been studied. Samples of the lingual papillae and lingual glands were collected from the tongue of an adult female lowland tapir. The microscopic analysis of the structure of the lingual papillae and the histochemical analysis of the secretion of the lingual glands were analyzed. The tongue of the tapir is divided into the apex, body with a distinct lingual prominence, and the root. Its ventral surface is smooth. The most numerous of the mechanical papillae were the filiform papillae, while numerous conical papillae with a sharp apex or more rounded papillae were present in the root of the tongue. There were also nine vallate papillae and pair of foliate papillae. The foliate papillae contained several folds parted by 12–14 grooves. The mucous secretion produced by the lingual glands was more obvious than the serous secretion. The features of the dorsal surface of the tongue as well as the shape and number of the lingual papillae on the surface of the tongue of the examined female tapir differ compared to Equidae or Rhinocerotidae, the other two representatives of Perissodactyla. However, further study is necessary for the synapomorpy of the tapir’s tongue.

scholarly journals Three dimensional structure of the connective tissue papillae of cat lingual papillae.

1988 ◽  
Vol 30 (6) ◽  
pp. 719-731 ◽  
Author(s):  
Kan Kobayashi ◽  
Ken Miyata ◽  
Shin-ichi Iwasaki ◽  
Keiichi Takahashi
Keyword(s):  
Connective Tissue ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Lingual Papillae

scholarly journals Determination of Effect on Cutaneous Wound Healing of Ozonated Hazelnut Oil

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1537
Author(s):  
Sibel Bayil Oğuzkan ◽  
Salih Tunç Kaya ◽  
Aziz Cesur ◽  
Bora Karagül ◽  
Serpil Uğraş ◽  
...  
Keyword(s):  
Wound Healing ◽  
Digital Camera ◽  
Dorsal Surface ◽  
Hazelnut Oil ◽  
Sprague Dawley ◽  
Experimental Animals ◽  
Sprague Dawley Rats ◽  
Package Program ◽  
The Stability ◽  
Marked Area

In this study, the wound healing effect of ozonated hazelnut oil was investigated on 54 male Sprague-Dawley rats. 7 experimental groups were designed. A circular area with a diameter of approximately 2 cm on dorsal surface was drawn using a coin and full thickness of the marked area was cut approximately 3 cm away from the ears by sterile scissors and forceps. All oils were daily applied on wound surface of experimental animals in volume of 0.2 mLduring 15 days. In this study, statistical analysis of the data was performed using the SPSS 22.0 package program. Wounds of all experimental animals were photographed by a digital camera on days 0, 3, 6, 12 and 15 after wounding. Ozonated oil treated wounds had significantly higher than the other groups on the day 3 after wounding. There is a quantitatively healing is determined on 3 day on rats. It has been observed in the stability period that ozonated oils must be stored at a temperature below 4 °C. Vitamin E should be added as an additive in the medical use of ozonated hazelnut oil in wound healing.

The pathology induced in laboratory rats by metacestodes ofTaenia crassicepsandMesocestoides corti(Cestoda)

Parasitology ◽  
1983 ◽  
Vol 87 (2) ◽  
pp. 279-287 ◽  
Author(s):  
J. Chernin ◽  
Diane J. McLaren
Keyword(s):  
Mast Cells ◽  
Connective Tissue ◽  
Peritoneal Cavity ◽  
Body Wall ◽  
Sprague Dawley ◽  
Connective Tissues ◽  
Liver Lobe ◽  
Left Liver Lobe ◽  
Laboratory Rats ◽  
Sprague Dawley Rats

SUMMARYSprague–Dawley rats were infected by intraperitoneal injection with either the cysticerci ofTaenia crassicepsor the tetrathyridia ofMesocestoides corti. Both species of metacestodes reproduce asexually in the peritoneal cavity. The effect ofT. crassicepsupon the rats was to cause chronic inflammation of the peritoneum, fibrous adhesions between the visceral organs and hyperplasia of the gut mesenteries which became extended into a large sac-like structure. Dead parasites surrounded by white calcareous material were found free in the peritoneum and attached to the inner body wall and viscera. The histopathology of the hyperplastic mesenteries and fibrous membrane is described. The tetrathyridia ofM. cortiwere found free in the peritoneal cavity as well as in the liver (mainly the left liver-lobe), gut mesenteries and in an outgrowth of the connective tissue associated with the pancreas. The connective tissue outgrowth comprised 2 layers; an inner reticular layer and a thickened marginal layer. Metacestodes were found in collagen—fibrin capsules within the connective tissue. A layer of mucopolysaccharide material was interposed between the parasite and the capsule wall. A possible method by which the parasites gain entry into the connective tissue is described. The connective tissues of rats harbouring eitherM. cortiorT. crassicepshad increased numbers of mast cells compared to connective tissues from control rats. Younger mast cells containing biogenic amines predominated in infected tissues whereas older heparin-containing cells dominated control tissues.

Vasopressin actions on area postrema neurons in vitro

1995 ◽  
Vol 269 (2) ◽  
pp. R463-R468
Author(s):  
V. L. Lowes ◽  
K. Sun ◽  
Z. Li ◽  
A. V. Ferguson
Keyword(s):  
Area Postrema ◽  
Brain Slices ◽  
Dorsal Surface ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Artificial Cerebrospinal Fluid ◽  
Single Unit Recordings ◽  
Excitatory Responses ◽  
Dose Dependent

The area postrema (AP) is a circumventricular organ located on the dorsal surface of the medulla. Substantial evidence suggests that the AP is an important site involved in cardiovascular regulation. Arginine vasopressin (AVP) is thought to act at the AP to increase the sensitivity of the baroreceptor reflex. We have therefore examined the effects of AVP on AP neurons with the use of extracellular single unit recordings in vitro. Coronal medullary brain slices (thickness = 400 microns) were obtained from male Sprague-Dawley rats and maintained in oxygenated artificial cerebrospinal fluid (aCSF). The slices were perfused with AVP (10(-8) to 10(-6) M), and the effect on single AP neurons was recorded. A total of 79 AP neurons was tested of which 50 (63.3%) were excited by AVP and 5 (6.3%) were inhibited, whereas the remaining 24 (30.3%) cells were unaffected. The excitatory effects of AVP were dose dependent: firing rate increased 92.6 +/- 25.8% at 10(-8) M, 289.4 +/- 53.9% at 10(-7) M, and 456.8 +/- 113.1% at 10(-6) M, respectively. We also examined whether these effects of AVP resulted from direct actions of this peptide on AP cells by testing if responses were retained during blockade of synaptic transmission (achieved by perfusion with a low Ca(2+)-high Mg2+ aCSF) in 11 cells excited by AVP. Nine of these cells were excited by AVP during such synaptic blockade. Finally, we demonstrated that the excitatory responses of five AP cells to AVP were all totally abolished by perfusion of slices with aCSF containing the V1 antagonist ([1-beta-mercapto-beta,beta-cyclopentamethylene propionic acid,2-(O-methyl)tyrosine]-Arg8-vasopressin; Peninsula Laboratories, 10(-6) M).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Morphological Characteristics of Renal Artery and Kidney in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Atilla Yoldas ◽  
Mustafa Orhun Dayan
Keyword(s):  
Renal Artery ◽  
Wistar Rats ◽  
Three Dimensional ◽  
Morphological Characteristics ◽  
Gross Anatomy ◽  
Renal Arteries ◽  
Sprague Dawley ◽  
Body Weights ◽  
The Mean ◽  
The Right

The gross anatomy and morphometry of the kidney and renal arteries were studied in the strains of laboratory rat: Sprague-Dawley (Sp) and Wistar (W) rats. Total of 106 three-dimensional endocasts of the intrarenal arteries of kidney that were prepared using standard injection-corrosion techniques were examined. A single renal artery was observed in 100% of the cases. The renal arteries were divided into a dorsal and a ventral branch. The dorsal and ventral branches were divided into two branches, the cranial and caudal branch. Renal arteries were classified into types I and II, depending on the cranial and caudal branches and their made of branching. The present study also showed that the right kidney was slightly heavier than the left one and that the kidney of the male was generally larger than that of the female. The mean live weights of the Sprague-Dawley and Wistar rats were found to be 258.26 ± 5.9 and 182.4 ± 19.05 g, respectively. The kidney weights were significantly correlated (P<0.01) with body weights. The kidney weights were not found significantly correlated (P>0.01) with the length of renal arteries.

scholarly journals The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue’s Sprague dawley Rats

2014 ◽  
Vol 1 (2) ◽  
pp. 78 ◽  
Author(s):  
Kwartarini Murdiastuti ◽  
Suryono Suryono ◽  
Aini Moeljono ◽  
Mefi Priba Sari ◽  
Rani Gamawati
Keyword(s):  
Connective Tissue ◽  
Visible Light ◽  
Sprague Dawley ◽  
Epithelial Thickness ◽  
Anterior Teeth ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Hematoxylin Eosin

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd  (group C), 5th  (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.

scholarly journals Application of an Ultrasound Semi-Quantitative Assessment in the Degradation of Silk Fibroin Scaffolds In Vivo

2021 ◽  
Author(s):  
Lihui Cai ◽  
Nan Gao ◽  
Tingyu Sun ◽  
Ke Bi ◽  
Xin Chen ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
Quantitative Detection ◽  
Sprague Dawley ◽  
Cross Sectional ◽  
Sprague Dawley Rats ◽  
The Cross ◽  
Scaffold Degradation ◽  
Living Organisms

Abstract Background: Research on the degradation of silk fibroin (SF) scaffolds in vivo lacks uniform and effective standards and experimental evaluation methods. This study aims to evaluate the application of ultrasound in assessing the degradation of SF scaffolds.Methods: Two groups of three-dimensional regenerated SF scaffolds (3D RSFs) were implanted subcutaneously into the backs of Sprague-Dawley rats. B-mode ultrasound and hematoxylin and eosin (HE) staining were performed on days 3, 7, 14, 28, 56, 84, 112, 140, and 196. The cross-sectional areas for two groups of 3D RSFs that were obtained using these methods were semi-quantitatively analyzed and compared to evaluate the biodegradation of the implanted RSFs.Results: Semi-quantitative analysis of the cross-sectional areas detected using B-mode ultrasound revealed that the degradations of the two 3D RSF groups were significantly different. The degradation rate of the SF-B group was found to be higher than that of the SF-A group. This was consistent with the semi-quantitative detection results for HE staining. Regression analysis showed that the results of the B-mode ultrasound and HE staining were correlated in both groups, indicating that B-mode ultrasound is a reliable method to evaluate the SF scaffold degradation in vivo. As the SF scaffold degraded, its echo gradually decreased. In the early stages of degradation, the echo of the SF scaffold was higher than that of the muscle. In the middle stage of degradation, the echo was equal to that of the muscle. In the later stage, the echo was lower than that of the muscle.Conclusions: This study suggests that B-mode ultrasound can clearly display the implanted SF scaffolds non-invasively and monitor the degradation of the different SF scaffolds after implantation in living organisms in real-time.

scholarly journals Histomorphological investigation of tongue of Porcupine Hystrix cristate

2018 ◽  
Vol 42 (1) ◽  
pp. 12-17
Author(s):  
Abdularazzaq Baqer Kadhim
Keyword(s):  
Connective Tissue ◽  
Lateral Surface ◽  
Dorsal Surface ◽  
Taste Buds ◽  
The Body ◽  
Caudal Part ◽  
Loose Connective Tissue ◽  
Circumvallate Papillae ◽  
Stratified Squamous Epithelium ◽  
Filiform Papillae

     Anatomical and histological explorations of the tongue in ten adult male Porcupine (Hystrix cristata) collected from AL-Diwanyha city by the hunter, after porcupine prepared the tongue illustrious after that the position, shape dimensions were enrolled. The square-shape of body, thicken less steadily toward caudal part of tongue. Root was tends caudally in the direction of the epiglottis. Torus linguae found on the caudal part of the dorsal surface of the tongue. In the end of lateral surface of tongue have foliate papilla osculate the premolar teeth. The length, apex, body and root of the tongue were (7.6±0.66, 1.25±0.23, 4.95±0.2, and 1.4±0.26 cm), respectively. The width, apex, body, and root of the tongue were (1.80±0.77, 0.35±0.49, 1±0.22 and 0.45±0.14, respectively. In the dorsal and lateral surface have muscle fibers from skeletal type and loose connective tissue bounded by keratinized stratified squamous epithelium while in the ventral lingual surface were non-keratinized. In the dorsal surface of body there were filiform papillae, but less at the lateral surface of the body. While the shape of circumvallate papillae was similar to furrow, and there was taste buds on the both dorsal and lateral sides of the tongue. Fungiform papillae had wide curved keratinized surface, with taste buds on the dorsal surface. Lamina propria and submucosa was loose connective tissue with more of collagen fiber.

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