scholarly journals Anti-Inflammatory and Antihyperalgesic Activities of Ethanolic Extract and Fruticulin A fromSalvia lachnostachysLeaves in Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ana Claudia Piccinelli ◽  
Diana Figueiredo de Santana Aquino ◽  
Priscila Neder Morato ◽  
Ângela Midori Kuraoka-Oliveira ◽  
Regiane Lauriano Batista Strapasson ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Mechanical Hyperalgesia ◽  
Protein Extravasation ◽  
Analgesic Effects ◽  
Carrageenan Injection ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Pleural Lavage

The anti-inflammatory and analgesic effects of the ethanolic extract (SLEE) and fruticulin A from the leaves ofSalvia lachnostachyswere evaluated in mice, using experimental models of inflammation (paw oedema and pleurisy induced by carrageenan injection) and hyperalgesia (electronic Von Frey). Oral administration of SLEE (30, 100, and 300 mg/kg) and fruticulin A (0.3 and 3.0 mg/kg) decreased the total leucocytes number in pleural lavage, protein extravasation, and paw oedema. SLEE (100 mg/kg) and fruticulin A (3 mg/kg) also exhibited antihyperalgesic activity in carrageenan induced mechanical hyperalgesia. In addition, fruticulin A (3 mg/kg) prevented mechanical hyperalgesia, inhibiting TNF but not L-DOPA-induced mechanical hyperalgesia. In conclusion, SLEE and fruticulin A display anti-inflammatory and analgesic properties. Therefore, fruticulin A is at least partially responsible for the activity observed in the ethanolic extract ofSalvia lachnostachys.

scholarly journals Evaluation of analgesic and anti-inflammatory activities of ethanolic extract of Cordia sebestena L.

2019 ◽  
Vol 66 (2) ◽  
pp. 26-31
Author(s):  
H. Sunaryo ◽  
S. Siska ◽  
E. Hanani ◽  
RS. Anindita ◽  
N. Yanti ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Experimental Models ◽  
Albino Rats ◽  
Clinical Settings ◽  
Dependent Manner ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Wistar Albino Rats

Abstract Trees and shrubs of the genus Cordia are widely distributed in the warmer regions, including Indonesia. The aim of this study was to evaluate the analgesic and anti-inflammatory properties of the ethanolic extract of plant leaves in Wistar albino rats. The analgesic activity was evaluated using the hot plate method and acetic acid-induced writhing, and the anti-inflammatory activity was determined using carrageenan-induced paw oedema. The results showed that the Cordia sebestena ethanol extract (100, 200 and 400 mg/kg) exhibited significant analgesic effects in a dose-dependent manner in the two pain models tested. The extract also exhibited significant anti-inflammatory effects in the carrageenan-induced inflammation test. The data obtained support the traditional folklore therapeutic claim about its analgesic and anti-inflammatory properties. Nonetheless, further scientific investigation is required to establish its analgesic and anti-inflammatory properties in other experimental models and clinical settings.

scholarly journals Serjania marginata Casar. hydroalcoholic extract reduced cytokine and inflammatory parameters in experimental models of inflammation and infection in mice

2021 ◽  
Author(s):  
Maicon Matos Leitão ◽  
Joyce Alencar Santos Radai ◽  
Luis Fernando Benitez Macorini ◽  
Thiago Leite Fraga ◽  
Silvia Cristina Heredia Vieira ◽  
...  
Keyword(s):  
Experimental Models ◽  
Mechanical Hyperalgesia ◽  
Paw Edema ◽  
Hydroalcoholic Extract ◽  
Inflammatory Parameters ◽  
Anti Inflammatory ◽  
Mycobacterial Growth ◽  
Pleural Lavage

Abstract This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of EESM in in vitro and in vivo models. EESM (0.98–1000 µg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 µg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 µg/mL while EESM did not interfere with against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCG-induced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

scholarly journals An Ethanolic Extract of Boehmeria caudata Aerial Parts Displays Anti-inflammatory and Anti-tumor Activities

2020 ◽  
Vol 7 (01) ◽  
pp. e17-e25 ◽  
Author(s):  
Paula Pereira de Paiva ◽  
Fabiana Regina Nonato ◽  
Ana Lúcia Tasca Gois Ruiz ◽  
Ilza Maria de Oliveira Sousa ◽  
Rafael Rosolen Teixeira Zafred ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
New Drugs ◽  
Antiproliferative Effects ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Inflammatory Action ◽  
Effective Source

AbstractThe tumor microenvironment presents several therapeutic targets, with inflammation being one of them. In search of new drugs, plants have shown to be an effective source of potent anti-inflammatory and anticancer agents. This study aimed to evaluate the antitumoral and inflammatory activities of Boehmeria caudata aerial parts extract. Bioguided in vitro antiproliferative screening showed that phenanthroquinolizidine obtained from the aerial B. caudata ethanolic extract had a straight relationship with activity. Moreover, the orally administered ethanolic extract reduced Ehrlich solid tumor growth and displayed an anti-inflammatory effect in both evaluated experimental models (carrageenan-induced paw edema and croton oil-induced ear edema). These results suggest that the antitumor activity of the ethanolic extract could be explained by antiproliferative effects associated with anti-inflammatory action.

scholarly journals Extract from Bougainvillea xbuttiana (Variety Orange) Inhibits Production of LPS-Induced Inflammatory Mediators in Macrophages and Exerts a Protective Effect In Vivo

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Rodolfo Abarca-Vargas ◽  
Vera L. Petricevich
Keyword(s):  
Inflammatory Mediators ◽  
Ethanolic Extract ◽  
Peritoneal Macrophages ◽  
Experimental Models ◽  
Nitric Oxide Release ◽  
Lps Challenge ◽  
Secretion Of Mediators ◽  
Anti Inflammatory

Background. Different pharmacological properties, such as antioxidant, antiproliferative, and anti-inflammatory properties, have been described among natural products. We previously described that the Bougainvillea xbuttiana (Variety Orange) ethanolic extract (BxbO) has an anti-inflammatory effect; however, this action is not fully understood. In this study, the action of the BxbO extract on the secretion of inflammatory mediators in two experimental models, in vitro and in vivo, after LPS challenge was evaluated. Methods. Peritoneal macrophages were obtained from female BALB/c mice and LPS-challenged with or without the BxbO extract. For the evaluation of mediators, the supernatants at 0, 12, 24, 36, and 48 hours were collected. For in vivo estimation, groups of female BALB/c mice were first intraperitoneously injected with different amounts of LPS and later administered the oral BxbO extract (v.o.) for 144 hours. To understand the mechanism of action, sera obtained from mice were collected at 0, 2, 4, 8, 12, and 24 hours after LPS challenge (with or without BxbO) for the detection of mediators. Results. The results showed that, in both peritoneal macrophages and sera of mice treated with the BxbO extract 1 hour before or together with LPS challenge, proinflammatory cytokines and nitric oxide release were unquestionably repressed. In contrast, in both systems studied here, the IL-10 levels were elevated to 5 to 9 times. At lethal doses of LPS, the BxbO extract treatment was found to protect animals from death. Conclusions. The results revealed that the inhibitory, protective, and benign effects of the BxbO extract were due to its capacity to balance the secretion of mediators.

scholarly journals Cashew (Anacardium occidentale L.) Nuts Counteract Oxidative Stress and Inflammation in an Acute Experimental Model of Carrageenan-Induced Paw Edema

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 660 ◽  
Author(s):  
Marika Cordaro ◽  
Rosalba Siracusa ◽  
Roberta Fusco ◽  
Ramona D’Amico ◽  
Alessio Filippo Peritore ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Radical Scavenging ◽  
Oxygen Free Radical ◽  
Anacardium Occidentale ◽  
Oxidative Enzymes ◽  
Paw Edema ◽  
Analgesic Effects ◽  
Carrageenan Injection ◽  
Anti Inflammatory ◽  
The Right

Background: Anacardium occidentale L. is a medicinal plant with powerful anti-oxidative and anti-inflammatory properties. Acute inflammatory events cause tissue alterations, decrease of anti-oxidative endogenous enzymes such as superoxide dismutase, catalase and glutathione, neutrophils infiltration, increase in the activities of myeloperoxidase, malondialdehyde, and pro-inflammatory release. Methods: Paw edema was induced by subplantar injection of carrageenan into the right hind paw in rats, but 30 min before a group of animals were orally treated with 100 mg/kg of cashew nuts to evaluate the anti-inflammatory and anti-oxidative response. Results: In the present work, we found that (1) cashew nuts reduced the development of carrageenan-induced paw edema limiting the formation of edema and pain; (2) cashew nuts ameliorated the diminutions of the anti-oxidative enzymes caused by carrageenan injection; (3) cashew nuts decreased myeloperoxidase malondialdehyde activity induced by carrageenan; and (4) cashew nuts acted by blocking pro-inflammatory cytokines response and nitrate/nitrite formation stimulated by carrageenan injection. Conclusions: The mechanisms of anti-inflammatory and analgesic effects exerted by cashew nuts were relevant to oxygen free radical scavenging, anti-lipid peroxidation, and inhibition of the formation of inflammatory cytokines.

scholarly journals Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7353
Author(s):  
Nasir Aziz Wagay ◽  
Shah Rafiq ◽  
Mohammad Aslam Rather ◽  
Younas Rasheed Tantray ◽  
Feng Lin ◽  
...  
Keyword(s):  
Total Protein ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Total Lipids ◽  
Anti Inflammatory

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

scholarly journals Phytochemical and Pharmacological evaluation of hibiscus hispidissimus griff

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2063-2067
Author(s):  
Alekhya V ◽  
Ganapaty S ◽  
Deepan T
Keyword(s):  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Aerial Parts ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

To assess phytochemical with pharmacological studies of Hibiscus hispidissimus griff belong to family malavaceae. Preliminary phytochemical analysis reveals the presence of steroids, triterpenes, saponins, steroidal saponins and phenols. Evaluation of anti-inflammatory, anti-microbial with antioxidant action were performed on aerial parts of methanolic extract of Hibiscus hispidissimus. Invitro antioxidant activity was performed by 2, 2 -diphenyl- 1- picrylhydrazyl (DPPH) assay, hydroxy radical scavenging method and superoxide radical scavenging activity.The results of invitro antioxidant study reveal that % inhibition of H. hispidissimus  was higher compared to ascorbic acid. Anti-inflammatory studies were performed using carrageenan-induced rat paw oedema animal model, for anti-inflammatory studies, the extracts were compared with standards like indomethacin, and it shows a remarkable zone of inhibition ranging from 58.97 to 71.73 respectively. The anti-bacterial and antifungal activity of plant extracts were studied for the occurrence of inhibition zones. The activity was performed by the cup plate method. Ethanolic extract of H. Hispidissimus  shows significant anti-bacterial effect against S. Aureus, B. Subtilis, P. Vulgaris and E. coli using ciprofloxacin (50µg/ml) as standard.The extracts show remarkable inhibition of zone of inhibition, and results were compared with that of standard drugs against the organism tested. In conclusion, the ethanolic extract of H. hispidissimus  shows  significant antioxidant, anti-inflammatory and anti-bacterial properties.

scholarly journals Anti-inflammatory and analgesic activities of Vatavidhvamsana rasa, An Ayurvedic herbo-mineral formulation

2021 ◽  
Vol 12 (3) ◽  
pp. 669-673
Author(s):  
Mahesh S ◽  
Swapnil Y Chaudhari ◽  
Mukesh Nariya ◽  
Patgiri B J
Keyword(s):  
Analgesic Activity ◽  
Radiant Heat ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Analgesic Effects ◽  
Inflammatory Conditions ◽  
Paw Oedema ◽  
Heat Response ◽  
Anti Inflammatory

Background: Inflammation is a complex process mainly comprises of three phases namely acute, sub-acute and chronic. In acute inflammatory conditions fluid and granulocytic cells accumulate at the site of injury because of the changes in small blood vessels. This response often activates systemic response such as leucocytosis, protein catabolism, fever and synthesis of C-reactive protein, an acute-phase protein In Ayurvedic texts, several herbo-mineral preparations to fight against inflammation have been found; among them, Vatavidhvamsana Rasa (VVR) is one, which is said to be remarkable in inflammation condition. Aim and Objectives: To assess the anti-inflammatory and analgesic effects of VVR in pharmacologically validated models. Materials and methods: Wistar strain albino rats weighing 200 ± 20 of either sex were used in the study. VVR was administered at a dose of 22.5 mg/kg for the rat. Results: Vatavidhvamsana Rasa (VVR) showed a significant decrease in the carrageenan-induced paw oedema after 1-hour interval in comparison to the control group (P<0.05) and at 3 hours and 5 hours intervals it showed a non-significant decrease in carrageenan-induced paw oedema in albino rats.  In the analgesic activity, VVR treated group showed a significant increase in radiant heat response at 30 min. interval (P<0.05) and non-significant increase at 60 mins in comparison to initial and control group. Conclusion: The results concluded that Vatavidhvamsana Rasa has anti-inflammatory and analgesic activity. Accordingly, it can be used in the management of pain and inflammatory conditions. 

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