scholarly journals Peptidases from Latex ofCarica candamarcensisUpregulate COX-2 and IL-1 mRNA Transcripts againstSalmonella entericaser. Typhimurium-Mediated Inflammation

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Maria Taciana Ralph ◽  
Ayrles Fernanda Brandão Silva ◽  
Dayane Laíse da Silva ◽  
Danielle Cristina Oliveira do Nascimento ◽  
Diogo Manoel Farias da Silva ◽  
...  
Keyword(s):  
The Other ◽  
Animal Group ◽  
Sephadex Column ◽  
Hand Control ◽  
Mrna Transcripts ◽  
Cysteine Peptidases ◽  
Immunomodulatory Properties ◽  
Cox 2 ◽  
C 30 ◽  
Dose Dependent

The immunomodulatory properties of a mixture of cysteine peptidases (P1G10) obtained from the fruit lattice ofCarica candamarcensiswere investigated. P1G10 was obtained from fresh latex samples by chromatography in a Sephadex column and initially administered to Swiss mice (n=5; 1 or 10 mg/kg) via i.p. After 30 min, the mice were injected with carrageenan (0.5 mg/mouse) or heat-killedS.Typhimurium (107 CFU/mL; 100°C/30 min) into the peritoneal cavity. Afterwards, two animal groups were i.p. administered with P1G10 (n=6; 1, 5, or 10 mg/Kg) or PBS 24 hours prior to challenge with liveS.Typhimurium (107 CFU/mL). P1G10 stimulated the proliferation of circulating neutrophils and lymphocytes, 6 h after injection of carrageenan or heat-killed bacteria, respectively. Furthermore, survival after infection was dose-dependent and reached 60% of the animal group. On the other hand, control mice died 1–3 days after infection. The examination of mRNA transcripts in liver cells 24 h after infection confirmed fold variation increases of 5.8 and 4.8 times on average for IL-1 and COX-2, respectively, in P1G10 pretreated mice but not for TNF-α, IL-10,γ-IFN and iNOS, for which the results were comparable to untreated animals. These data are discussed in light of previous reports.

Effects of over Heat and Casting Speed on Quality of GCr15 Billet

2014 ◽  
Vol 997 ◽  
pp. 534-537 ◽  
Author(s):  
Qi Wei Zuo ◽  
Xia An ◽  
Jing Bo Yang ◽  
Da Qiang Cang
Keyword(s):  
Casting Speed ◽  
The Other ◽  
Steel Making ◽  
Average Grade ◽  
Upset Forging ◽  
C 30 ◽  
Better Than ◽  
Center Porosity

In order to improve the quality of the billets, the whole test proceedings by the comparison of the two casting conditions which one is over heat 20°C-30°C under casting speed 0.4m/min-0.6m/min and the other is over heat 10°C-15 °C under casting speed 1.3m/min-1.6m/min are checked during the produce process of GCr15 in some domestic steel-making plant. The results show that the average grade of general porosity decreases 1.0, the average grade of center porosity down 0.5, the average grade of center segregation fall 0.5 and the samples tested in hot upset forging perform better than before. According to the results, the latter is determined to be accepted.

ATP stimulates Ca2+ release from a rapidly exchanging pool in cultured rat epididymal cells

1993 ◽  
Vol 264 (6) ◽  
pp. C1388-C1394 ◽  
Author(s):  
A. Y. Leung ◽  
H. L. Tai ◽  
P. Y. Wong
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
The Other ◽  
Free Solution ◽  
Tetraacetic Acid ◽  
Cl Secretion ◽  
Transient Rise ◽  
Extracellular Compartment ◽  
Dose Dependent Fashion ◽  
Dose Dependent

A study was carried out to investigate an ATP-sensitive Ca2+ pool in rat epididymal cells and its role in transepithelial Cl- secretion. In normal buffered solution containing 2.5 mM free Ca2+, ATP triggered single calcium spikes in a dose-dependent fashion. In nominally Ca(2+)-free solution, the peaks of successive Ca2+ spikes diminished after repeated ATP stimulations. Addition of Sr2+ (2.5 mM) to Ca(2+)-free solution after ATP stimulation did not cause changes in fluorescence signals. However, in the presence of Sr2+, ATP gave rise to apparent repetitive Ca2+ spikes of similar magnitudes after repeated stimulations. Increasing the time of exposure in Ca(2+)-free solution containing 50 microM ethylene glycol-bis(beta-amino-ethyl ether)-N,N,N',N'-tetraacetic acid rapidly decreased the intracellular Ca2+ concentration ([Ca2+]i) response to subsequent ATP stimulation. On the other hand, increasing the time of exposure in Sr(2+)-containing solution in Ca(2+)-depleted cells rapidly increased the apparent [Ca2+]i response to subsequent ATP stimulation. These observations suggested the existence of a Ca2+ pool that was rapidly exchanging with the extracellular compartment. Apical application of ATP elicited a transient rise in short-circuit current across the epididymal epithelium in a dose-dependent fashion, and the response was reduced by prior stimulation with thapsigargin. Ca2+ released from a rapidly exchanging ATP-sensitive store might stimulate Cl- secretion in the epididymis, thereby maintaining the electrolyte contents and fluidity of the epididymal microenvironment.

scholarly journals Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats

2010 ◽  
Vol 25 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Vinicius von Diemen ◽  
Manoel Roberto Maciel Trindade
Keyword(s):  
Weight Gain ◽  
Oral Administration ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
The Other ◽  
Control Group ◽  
Pregnant Rats ◽  
Dependent Relation ◽  
Dose Dependent

PURPOSE: Determine the effects of the MSG (monosodium glutamate) in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length) and IL (Index of Lee) at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (p<0,05) and with 21 days of life presented weight and NAL lower than the groups G10 and this lower than the GC (p<0,01). Otherwise the G20 at 21 days of life had the IL similar to the other two groups. The weight profit percentage from birth to the 21st day of life was lower in the G20 regarding the other two groups (p<0,01). The G20 had a NAL increase percentage from birth to the 21st day of life lower than the G10 and this lower than the GC (p<0,01). CONCLUSIONS: MSG presented a dose-dependent relation in the variables weight and NAL. It caused a decrease in the growth pattern as well as in the weight gain pattern until the 21st day of life. The IL of the group 20% had an increased in relation to the control group after 3 weeks of follow up.

scholarly journals Investigation into the Effects of Backrest Angle and Stick Location on Female Strength

2021 ◽  
Vol 19 (1) ◽  
pp. 7
Author(s):  
Victor Ei-Wen Lo ◽  
Shu-Min Chao
Keyword(s):  
Musculoskeletal Disorders ◽  
Muscular Strength ◽  
The Other ◽  
Maximum Strength ◽  
Hand Strength ◽  
Federal Regulations ◽  
Independent Variables ◽  
The Us ◽  
Hand Control ◽  
Abduction Strength

Objectives: The purpose of this study was to investigate the effects of backrest angle and hand maneuver direction on maximum hand strength and to recommend a strength value for the hand-controlled stick of an aircraft. Methods: Forty-eight female subjects were recruited to perform simulated forward–backward and adduction–abduction maneuvers using control sticks. Each subject was free from musculoskeletal disorders and pain. The independent variables included four control maneuvers (forward, backward, adduction, abduction), two right-hand control stick locations (central, side), and three backrest angles (90°, 103°, 108°). The dependent variable was maximum hand strength. Results: The maximum strength for forward maneuvers with both central and side sticks was strongest at a 90° backrest angle (p < 0.001). The maximum strength for adduction maneuvers with both central and side sticks was also strongest at a 90° backrest angle (p < 0.001). On the other hand, the highest strength was observed at a 108° backrest angle when pulling the stick backward (p < 0.001). The abduction strength was significantly stronger than the adduction strength with a central stick (p < 0.001), but the adduction strength was significantly stronger than the abduction strength with a side stick (p < 0.001–p = 0.017). The forward and abduction strength were significantly different in different locations (p < 0.001). The recommended strength in the Code of Federal Regulations (CFR) by the US FAA is higher than the strength values observed in this study. Conclusions: The backrest angle, directions, and location affected the muscular strength. The recommended values should be reevaluated and adjusted for Taiwanese pilots.

scholarly journals Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9438
Author(s):  
Eduardo Cienfuegos-Pecina ◽  
Tannya R. Ibarra-Rivera ◽  
Alma L. Saucedo ◽  
Luis A. Ramírez-Martínez ◽  
Deanna Esquivel-Figueroa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Succinic Acid ◽  
Wistar Rats ◽  
Ischemia Reperfusion ◽  
The Other ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Female Wistar Rats ◽  
Dose Dependent ◽  
Nephroprotective Effect

Background Ischemia–reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (S)-2-hydroxyglutarate [(S)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats. Methods (S)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (diazotization/alkaline hydrolysis), and its structure was confirmed by nuclear magnetic resonance and polarimetry. SA was acquired commercially. (S)-2HG and SA were independently evaluated in male and female Wistar rats respectively after renal IR injury. Rats were divided into the following groups: sham (SH), nontoxicity [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg], IR, and compound+IR [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg]; independent SH and IR groups were used for each assessed compound. Markers of kidney injury (BUN, creatinine, glucose, and uric acid) and liver function (ALT, AST, ALP, LDH, serum proteins, and albumin), proinflammatory cytokines (IL-1β, IL-6, and TNF-α), oxidative stress biomarkers (malondialdehyde and superoxide dismutase), and histological parameters (tubular necrosis, acidophilic casts, and vascular congestion) were assessed. Tissue HIF-1α was measured by ELISA and Western blot, and the expression of Hmox1 was assessed by RT-qPCR. Results (S)-2HG had a dose-dependent nephroprotective effect, as evidenced by a significant reduction in the changes in the BUN, creatinine, ALP, AST, and LDH levels compared with the IR group. Tissue HIF-1α was only increased in the IR group compared to SH; however, (S)-2HG caused a significant increase in the expression of Hmox1, suggesting an early accumulation of HIF-1α in the (S)-2HG-treated groups. There were no significant effects on the other biomarkers. SA did not show a nephroprotective effect; the only changes were a decrease in creatinine level at 12.5 mg/kg and increased IR injury at 50 mg/kg. There were no effects on the other biochemical, proinflammatory, or oxidative stress biomarkers. Conclusion None of the compounds were hepatotoxic at the tested doses. (S)-2HG showed a dose-dependent nephroprotective effect at the evaluated doses, which involved an increase in the expression of Hmox1, suggesting stabilization of HIF-1α. SA did not show a nephroprotective effect but tended to increase IR injury when given at high doses.

scholarly journals Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis

2020 ◽  
Vol 37 (2) ◽  
pp. 123-127
Author(s):  
Kyeong Ju Park ◽  
Ho-Sueb Song
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Treated Group ◽  
Bee Venom ◽  
Related Protein ◽  
Griess Reaction ◽  
Cox 2 ◽  
Dose Dependent Decrease ◽  
Dose Dependent ◽  
Inflammatory Effect

Background: This study was designed using a mouse model of atopic dermatitis [phthalic anhydride (PA)-treated mice], to investigate the anti-inflammatory effect of bee venom pharmacopuncture (BVP) in keratinocytes.Methods: Western blot analysis was performed to investigate inflammation related protein expression of iNOS, COX-2, phospho-ERK (p-ERK), and ERK, in LPS (1 μg/mL)-activated keratinocytes, following BVP treatment, and in PA-treated mice, after BVP treatment. Griess reaction was performed to investigate NO concentration. Enzyme-linked immunosorbent assays were used to determine the concentrations of interleukin (IL)-4+, IL-17A+, IL-13 and IL-4 in PA-treated mice after BVP treatment. In addition, monocyte, macrophage, neutrophil, and eosinophil counts were measured to observe the changes in white blood cell infiltration.Results: The keratinocytes of the BVP-treated group showed a decreased expression of iNOS, COX-2, ERK at 5 OX-2, ERK E, and p-ERK at 1, 2 and 5 RKRK ERK ERK, and a dose-dependent decrease in NO concentration at 2 and 5 ntrationof s. In the BVP-treated groups (0.1 μ.1-trea μ.1-treated gr), PA-treated mice showed recovery after 4 weeks which was dose-dependent, showing a significant decrease in clinical scores for AD, and a decreased concentration of IL-13 and IL-4 with BV treatment. There was a dose-dependent decrease in the infiltration of eosinophils, neutrophils, monocytes, macrophages, and a decreased thickness of the epidermis due to inflammation, and decreased expressions of iNOS, COX-2, p-ERK, ERK, especially in the 0.1 μ0/mL BVP-treated group,<br>Conclusion: These results suggest that BVP may be an effective alternative treatment for atopic dermatitis.

scholarly journals COMMUNICATION STRATEGY MODEL IN DIRECTORATE ICT AS FOR DIGITAL UNIVERSITY 2023

Gunahumas ◽  
2020 ◽  
Vol 2 (1) ◽  
pp. 283-292
Author(s):  
Deni Darmawan
Keyword(s):  
Swot Analysis ◽  
Legal Protection ◽  
Communication Strategy ◽  
The Other ◽  
System Component ◽  
Resources Management ◽  
Strategy Model ◽  
Hand Control ◽  
Work Units ◽  
Important System

Abstrak Analisis ketercapaian dan hambatan serta pengoptimalan potensi dan kekuatan yang dimiliki Dir. TIK sekarang ini. Dengan demikian hal ini berdampak kepada Kredibilitas Dir.TIK yang harus dipertahankan yang bagusnya dan ditingkatkan yang lemahnya sesuai visi dan misi serta tupoksi sebagai komponen system penting dari UPI. Di sisi lain Kontrol keseimbangan terhadap peran dan warna dari produk inovasi di lingkungan Dir. TIK yang harus ditingkatkan dalam menopang semua unit kerja di UPI. Untuk itu diperlukan upaya seperti menganalisis peluang-peluang peran yang lebih luas dari yang sudah ada selama ini di lingkungan Dir. TIK untuk mewujudkan visi dan misi UPI. Diantara program kegiatannya bias dimulai dengan : (a) memetakan Sejumlah program kerja yang memiliki target jelas sesuai hasil analisis SWOT terhadap semua sumber daya, manjerial, dan payung hukum yang berlaku; (b) Memulai merumuskan RKAT yang bersih, terbuka dan mudah diakses dengan cepat; dan (c) Bekerjasama dengan unit kerja terdepan seperti HUMAS, PPID dan ULT, serta semua LO di 52 unit kerja di lingkungan UPI. Kata Kunci : Direktorat TIK; PPID; ULT Abstract Analysis of achievements and obstacles as well as optimizing the potential and strength of Dir. ICT now. Thereby this has an impact on the credibility of Dir.TIK which must be maintained which is good and improved which is weak according to the vision and mission and duties as an important system component of UPI. On the other hand Control the balance of the role and color of innovation products in the Dir environment. ICTs must be improved in supporting all work units at UPI. For this reason efforts are needed such as analyzing opportunities for roles that are broader than those currently available in the Dir environment. ICT to realize the vision and mission of UPI. Among the program activities can be started by: (a) mapping a number of work programs that have clear targets according to the results of the SWOT analysis of all applicable resources, management, and legal protection; (b) Beginning to formulate a clean, open and easily accessible RKAT; and (c) Collaborate with leading work units such as HUMAS, PPID and ULT, as well as all LO in 52 work units within UPI. Keywords : Directorate of ICT; PPID; ULT

scholarly journals New Perspectives on Aspirin and the Endogenous Control of Acute Inflammatory Resolution

2006 ◽  
Vol 6 ◽  
pp. 1048-1065 ◽  
Author(s):  
Thea Morris ◽  
Melanie Stables ◽  
Derek W. Gilroy
Keyword(s):  
Mode Of Action ◽  
Acute Inflammation ◽  
Lipid Mediators ◽  
The Other ◽  
Endogenous Control ◽  
Protective Properties ◽  
Traditional Mode ◽  
Inflammatory Drugs ◽  
Cox 2 ◽  
Anti Inflammatory

Aspirin is unique among the nonsteroidal anti-inflammatory drugs in that it has both anti-inflammatory as well as cardio-protective properties. The cardio-protective properties arise form its judicious inhibition of platelet-derived thromboxane A2over prostacyclin, while its anti-inflammatory effects of aspirin stem from its well-established inhibition of prostaglandin (PG) synthesis within inflamed tissues. Thus aspirin and the other NSAIDs have popularised the notion of inhibiting PG biosynthesis as a common anti-inflammatory strategy based on the erroneous premise that all eicosanoids are generally detrimental to inflammation. However, our fascination with aspirin has shown a more affable side to lipid mediators based on our increasing interest in the endogenous control of acute inflammation and in factors that mediate its resolution. Epi-lipoxins (epi-LXs), for instance, are produced from aspirin’s acetylation of inducible cyclooxygenase 2 (COX-2) and together with Resolvins represent an increasingly important family of immuno-regulatory and potentially cardio-protective lipid mediators. Aspirin is beginning to teach us what nature knew all along – that not all lipid mediators are bad. It seems that while some eicosanoids are pathogenic in a variety of diseases, others are unarguable protective. In this review we will re-count aspirin’s colorful history, discuss its traditional mode of action and the controversies associated therewith, as well as highlight some of the new pathways in inflammation and the cardiovascular systems that aspirin has recently revealed.

scholarly journals Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Corydalis Cava (Fumariaceae)

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Jakub Chlebek ◽  
Kateřina Macáková ◽  
Lucie Cahlíková ◽  
Milan Kurfürst ◽  
Jiří Kuneš ◽  
...  
Keyword(s):  
Human Blood ◽  
Inhibitory Activity ◽  
Potent Inhibitor ◽  
The Other ◽  
Spectroscopic Techniques ◽  
Dependent Manner ◽  
Isoquinoline Alkaloids ◽  
Chemical Structures ◽  
Inhibitory Compounds ◽  
Dose Dependent

Tubers of Corydalis cava were extracted with ethanol and fractionated using n-hexane, chloroform and ethanol. Repeated column chromatography, preparative TLC and crystallization led to the isolation of fifteen isoquinoline alkaloids. The chemical structures of the isolated compounds were determined on the basis of spectroscopic techniques and by comparison with literature data. All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. (+)-Canadaline inhibited acetylcholinesterase as well as butyrylcholinesterase in a dose-dependent manner with IC50 values of 20.1 ± 1.1 μM and 85.2 ± 3.2 μM, respectively. (+)-Canadine, with an IC50 value of 12.4 ± 0.9 μM, was the most potent inhibitor of acetylcholinesterase, whilst (±)-corycavidine and (+)-bulbocapnine were effective inhibitors of butyrylcholinesterase with IC50 values of 46.2 ± 2.4 uM and 67.0 ± 2.1 μM, respectively. The other isolated alkaloids were considered inactive (IC50 > 100 μM).

scholarly journals The Effect of Systemic and Regional Use of Magnesium Sulfate on Postoperative Tramadol Consumption in Lumbar Disc Surgery

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Melek Demiroglu ◽  
Canan Ün ◽  
Dilsen Hatice Ornek ◽  
Oya Kıcı ◽  
Ali Erdem Yıldırım ◽  
...  
Keyword(s):  
Side Effects ◽  
Postoperative Analgesia ◽  
Lumbar Disc ◽  
Demographic Data ◽  
The Other ◽  
Lumbar Disc Surgery ◽  
Paraspinal Muscles ◽  
Pain Scores ◽  
Disc Surgery ◽  
C 30

Aim.To investigate the effect of magnesium administered to the operative region muscle and administered systemically on postoperative analgesia consumption after lumbar disc surgery.Material and Method.The study included a total of 75 ASA I-II patients aged 18–65 years. The patients were randomly allocated into 1 of 3 groups of 25: the Intravenous (IV) Group, the Intramuscular (IM) Group, and the Control (C) Group. At the stage of suturing the surgical incision site, the IV Group received 50 mg/kg MgSO4intravenously in 150 mL saline within 30 mins. In the IM Group, 50 mg/kg MgSO4in 30 mL saline was injected intramuscularly into the paraspinal muscles. In Group C, 30 mL saline was injected intramuscularly into the paraspinal muscles. After operation patients in all 3 groups were given 100 mg tramadol and 10 mg metoclopramide and tramadol solution was started intravenously through a patient-controlled analgesia device. Hemodynamic changes, demographic data, duration of anesthesia and surgery, pain scores (NRS), the Ramsay sedation score (RSS), the amount of analgesia consumed, nausea- vomiting, and potential side effects were recorded.Results.No difference was observed between the groups. Nausea and vomiting side effects occurred at a rate of 36% in Group C, which was a significantly higher rate compared to the other groups (p<0.05). Tramadol consumption in the IM Group was found to be significantly lower than in the other groups (p<0.05).Conclusion.Magnesium applied to the operative region was found to be more effective on postoperative analgesia than systemically administered magnesium.

Sign in / Sign up

Export Citation Format

Share Document