scholarly journals Retracted: Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates

2014 ◽  
Vol 2014 ◽  
pp. 1-1
2011 ◽  
Vol 122 (7) ◽  
pp. 1331-1338 ◽  
Author(s):  
Shen Chen ◽  
Xinqiong Liu ◽  
Liexian Zeng ◽  
Dongmei Ouyang ◽  
Jianyuan Yang ◽  
...  

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Endang Warih Minarni ◽  
Loekas Soesanto ◽  
Agus Suyanto ◽  
Rostaman

Abstract Background Brown plant hopper (Nilaparvata lugens Stal.) a very damaging pest to rice crops. One of the efforts to control it is the use of entomopathogenic fungi (EPF). Three fungal local isolates found in Indonesia were effective in controlling the brown plant hopper pest. This study aimed to molecularly identify the 3 fungal isolates. Molecular identification is very important to get the exact identity of these fungi. The accuracy of EPF identification will greatly determine the success of control. Molecular identification is based on a partial genetic analysis of the internal transcribed spacer (ITS) locus of ribosomal fungal DNA. Result Morphology of the local isolates named J22 and J60 were identified as Paecilomyces sp., while the isolate J34 was identified as Beauveria sp. The results of molecular identification of the isolates J22 and J60 were identified as the fungi Lecanicillium saksenae and Simplicillium sp., while isolate J34 was identified as Myrothecium sp. The results of literature search showed that the 3 fungi have never been previously reported to infect the brown plant hopper. Conclusion In Indonesia, 3 types of EPF, namely L. saksenae, Simplicillium sp., and Myrothecium sp., were found having the potential to control the brown plant hopper pest.


2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amos Onasanya ◽  
R. O. Onasanya ◽  
Abiodun A. Ojo ◽  
B. O. Adewale

Bacterial leaf blight (BLB) of rice is a very destructive disease worldwide and is caused by Xanthomonas oryzae pv. oryzae (Xoo). The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr, and mildly virulent pathotype, MVr), 12 Operon primers that gave reproducible and useful genetic information were selected and used to analyze 50 Xoo isolates from 7 West African countries. Genetic analysis revealed two major Xoo virulence genotypes (Mta and Mtb) with Mta having two subgroups (Mta1 and Mta2). Mta1 (Vr1) subgroup genotype has occurrence in six countries and Mta2 (Vr2) in three countries while Mtb genotype characterized mildly virulence (MVr) Xoo isolates present in five countries. The study revealed possible linkage and correlation between phenotypic pathotyping and molecular typing of Xoo virulence. Xoo virulence genotypes were known to exist within country and there was evidence of Xoo pathogen migration between countries. Durable resistance rice cultivars would need to overcome both Mta and Mtb Xoo virulence genotypes in order to survive after their deployment into different rice ecologies in West Africa.


2013 ◽  
Vol 55 (5) ◽  
pp. 473-483 ◽  
Author(s):  
Bao-Hua Feng ◽  
Yang Yang ◽  
Yong-Feng Shi ◽  
Hai-Chao Shen ◽  
Hui-Mei Wang ◽  
...  

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