scholarly journals In Vitro Free Radical Scavenging Activity of a Wild Edible Mushroom, Sparassis crispa (Wulf.) Fr., from North Western Himalayas, India

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Madhavi Joshi ◽  
Anand Sagar
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Edible Mushroom ◽  
Western Himalayas ◽  
Antioxidant Efficiency ◽  
Sparassis Crispa ◽  
Wild Edible Mushroom ◽  
North Western

Bioactive compounds and antioxidant activity of methanolic extract of Sparassis crispa collected from North Western Himalayan region of India were analyzed. Phenolic content 11.14±0.08 mg tannic acid equivalent per g of the extract and flavonoids 1.96±04 mg catechin equivalent per g of the extract were recorded to be the major antioxidant components in this wild edible mushroom. Significant antioxidant efficiency on inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was observed when compared to standard antioxidant like L-ascorbic acid. IC50 value of the extract was 2.11 mg/mL. The findings suggest S. crispa as an easily accessible source of natural antioxidants.

scholarly journals Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Ethiopian Indigenous Medicinal Plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L.

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Suresh Kumar P. Nair ◽  
Kumar Ganesan ◽  
Henok Azalewor ◽  
Neethu Letha ◽  
Sharmila Gani
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Ocimum Basilicum ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Phytochemical Investigation

Antioxidants are the chemical substances which prevent the free radicals damage in the body. Numerous researches are going globally focussed on investigating natural antioxidants of plants origins. The aims of the present study were to evaluate preliminary phytochemical investigation and in vitro antioxidant activities of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Aqueous, benzene and hexane crude leaves extracts of O. lamiifolium and O. basilicum were subjected to qualitative phytochemical screening using standard procedures. In addition, we investigated the antioxidant potential of crude aqueous leaves extract of O. lamiifolium and O. basilicum using tests involving inhibition of superoxide anions, DPPH, H2O2, NO and ABTS. Preliminary phytochemical investigation for benzene, hexane and aqueous extracts found alkaloids, sterols, carbohydrate and glycosides, tannins and flavonoids. The fraction inhibition of lipid peroxide at the first stage of oxidation illustrated antioxidant activity of O. lamiifolium and O. basilicum as 90% and 88% compared to those of gallic acid (97%) and BHT (84%) respectively. Also, the aqueous leaves extract of O. lamiifolium and O. basilicum exhibited significant DPPH free radical scavenging activity, nitric acid free radical scavenging activity assay, superoxide anion scavenging activity, ABTS scavenging activity and hydrogen peroxide free radical scavenging assay. Our findings provide confirmation that the aqueous leaves extract of O. lamiifolium and O. basilicum are potential source of natural antioxidants, and this warranted its uses in traditional medicine systems.

In Vitro Antioxidant Activity of Pigment Extracted from Fruits of Padus racemosa and Padus virginiana

2011 ◽  
Vol 183-185 ◽  
pp. 565-569 ◽  
Author(s):  
Jian Ren ◽  
Zhen Yu Wang
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Reducing Power Assay

The total anthocyanin content (TAC) of pigment extracted from fruits of Padus racemosa and Padus virginiana was determined by pH-differential method. TAC in pigment from fruits of Padus racemosa was higher than that in Padus virginiana. The vitro antioxidant activity of the two kinds of pigment was evaluated by different assays, including DPPH• assay, ABTS•+assay, OH• assay and reducing power assay. The results showed that except for reducing power, pigment from fruit of Padus racemosa showed stronger ABTS•+, DPPH• and OH• radical scavenging activity than pigment from fruit of Padus virginiana. The study concluded that pigment extracted from fruit of Padus racemosa and Padus virginiana can be used as a source of natural antioxidants instead of synthetic antioxidants.

Antioxidant Properties of Natural p-Terphenyl Derivatives from the Mushroom Thelephora ganbajun

2004 ◽  
Vol 59 (5-6) ◽  
pp. 359-362 ◽  
Author(s):  
Wei-Min Yang ◽  
Ji-Kai Liu ◽  
Lin Hu ◽  
Ze-Jun Dong ◽  
Wan-Lin Wu ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Edible Mushroom ◽  
Scavenging Activity ◽  
Sod Activity ◽  
Phenyl Compounds ◽  
Hydrazyl Radical

The antioxidant activity in vitro of three poly(phenylacetyloxy)-substituted 1,1′:4′,1″-ter-phenyl compounds from the edible mushroom Thelephora ganbajun were investigated. The IC50 values of compounds 1-3 for lipid peroxidation in rat liver homogenate were 400, 48, 54 μᴍ, respectively. Compounds 1-3 increased superoxide dismutase (SOD) activity with EC50 values of 182, 74, 204 μᴍ. They were also assessed on the DPPH (1,1-diphenyl-2-picryl- hydrazyl) radical scavenging activity with EC50 values of 49, 1233, 55 μᴍ.

Polyphenols of Rosa L. Leaves Extracts and their Radical Scavenging Activity

2007 ◽  
Vol 62 (1-2) ◽  
pp. 32-38 ◽  
Author(s):  
Renata Nowak ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Antioxidant Activity ◽  
Ellagic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Total Phenolic

Antioxidant potential of Rosa L. leaves methanolic extracts was evaluated in vitro using a spectrophotometric method based on measuring the radical scavenging effect on 2,2-diphenyl- 1-picrylhydrazyl (DPPH) radicals. The contents of ellagic acid, quercetin and kaempferol in the extracts from leaves of seventeen rose species were determined using SPE-RPHPLC methods. Additionally, total phenolic content was determined spectrophotometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). Remarkable high antioxidant activity and high total phenolic content (5.7% < GAE < 15.2%), large ellagic acid (EA) content from 9.37 to 19.42 mg/g of dry weight, a quercetin content ranging from 3.68 to 15.81 mg/g of dry weight and kaempferol content from 1.25 to 9.41mg/g of dry weight were found in rose leaves. Significant correlation between EA (r2 = 0.6131), quercetin (r2= 0.5158), total phenolic content (r2= 0.8485) and antioxidant activity was observed. Basing on the studies conducted one may assume that the extracts of rose leaves are a rich source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects.

scholarly journals Evaluation of Phyto Chemical Biochemical and In Vitro Antioxidant Potential of Angelica Glauca Grown at High Altitude Areas of Western Himalayas

2021 ◽  
Vol 6 (2) ◽  
pp. 117-121
Author(s):  
Swati Arya ◽  
H.K. Pandey ◽  
A. Singh ◽  
H.S. Meena ◽  
M. Bala
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biochemical Parameter ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Western Himalayas ◽  
Hydroalcoholic Extract ◽  
Angelica Glauca

Angelica glauca Edgew is an important medicinal and aromatic herb (family Apiaceae). The roots of A. glauca commonly used as spices by local peoples. In the present study, the phytochemical constituents, biochemical parameters and, in-vitro antioxidant activity of A. glauca roots collected from the Himalayan region have been studied. For preliminary phytochemical analysis, the hydroalcoholic and aqueous root extract of Angelica glauca were screened for the presence of carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The biochemical parameter and in-vitro antioxidant potential of Angelica glauca were analysed by using standard methods. Phytochemical analysis of extract showed major classes of phytochemicals constituents such as carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The results from the current study demonstrated that A.glauca roots contained carbohydrate (21±0.72 %), crude protein (12.7±0.31 %), total ash (3.86±0.034 %), dietary fiber (18.9±0.14 %), total fat (4.5±0.38 %) and ascorbic acid (68.5±0.19mg/100g). The hydroalcoholic extract showed the highest quantity of total phenol, total flavonoids, and total tannin content compared to aqueous extract. The hydroalcoholic extract exhibited high DPPH radical scavenging activity (IC50=68.1±0.34 µg/ml). The result showed that A.glauca roots have high nutritional and antioxidant potential. Hence the plant can be used as a nutraceutical and natural antioxidant.

scholarly journals Antioxidant potential of Helichrysum plicatum DC. (Asteraceae)

2005 ◽  
Vol 51 ◽  
pp. 29-34 ◽  
Author(s):  
Tatjana Kadifkova Panovska ◽  
Svetlana Kulevanova
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Beta Carotene ◽  
Natural Antioxidants ◽  
Experimental Models ◽  
Antioxidant Potential ◽  
Experimental Systems ◽  
Preventive Activity ◽  
Β Carotene

The present study describes the ability of different extracts of H. plicatum obtained from flowers, stems and leaves, to act as natural antioxidants in different in vitro experimental models in which free radical reactions are involved: inhibition of DPPH (1,1-diphenyl-2- picrylhydrazyl) radical, inhibition of hydroxyl radicals and protection of β-carotene-linoleic acid model system. Investigate extracts showed radical scavenging activity with IC50 from 6 to 11 mg/ml. The extracts are capable to reacting with OH• radical with inhibition of its production ranged between 33-58%. The high preventive activity against the bleaching of beta-carotene (15-49% of initial value after 120 minutes) was also observed. The antioxidative activity of the extracts in the experimental systems was compared with that of reference substances: luteolin, quercetin, BHA, BHT and sylimarin (the main agent of the well-known milk thistle – Silybum marianum L.). Results of this study suggest that Helichrysum plicatum represent a natural source with antioxidant potential.

scholarly journals Antidiabetic and antioxidant potential of Zanthoxylum armatum DC. leaves (Rutaceae): An endangered medicinal plant

2020 ◽  
Vol 7 (1) ◽  
pp. 93-100
Author(s):  
Sana Khan ◽  
. Richa ◽  
Rinku Jhamta ◽  
Harsimran Kaur
Keyword(s):  
Plant Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Essential Elements ◽  
Antioxidant Potential ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Zanthoxylum Armatum

The present study was designed to evaluate the antidiabetic and antioxidant potential of methanolic extract of Zanthoxylum armatum leaves using in vitro approaches. The concentration of plant extract that inhibited 50% (IC50) of alpha amylase was found to be 89.37±4.68 ?g/ml which is higher than standard. Results of this study shows that 2,2-diphenyl-1-picrylhydrazyl scavenging test show high radical scavenging activity as compared to hydrogen peroxide scanvenging method with IC50 Value of 57.83 ?g/ml and 79.13 ?g/ml, respectively. Plant extract found to exhibit enormous amount of phenols and flavonoid content i.e., 140.71 mg GAE/g and 88.53 mg of Quercetin/g of extract respectively. Further phytochemical analysis revealed that plant exhibit glycosides, alkaloids, terpenoids, flavonoids, saponin and tannin that are frequently implicated as having antidiabetic effects. Elemental analysis revealed the presence of essential elements ‘Mg’, ‘Mn’, ‘Zn’, ‘Fe’, ‘K’, ‘P’, ‘Ca’, ‘Cu’, ‘Mo’ and ‘Ni’ known to play role in regulating blood glucose. It could be speculated that the observed antidiabetic activity of Z. armatum might be related to the presence of these phytochemicals, phenolic compounds as well as mineral elements which found to be the important constituent of Z. armatum. These results indicate that Z. armatum could be an excellent source of natural antioxidants and exhibited antidiabetic activity.

scholarly journals Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)

10.5219/1579 ◽  
2021 ◽  
Vol 15 ◽  
pp. 784-791
Author(s):  
Yulia Vinogradova ◽  
Olga Shelepova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Jan Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Echinocystis Lobata

Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.

CHEMO-PROFILING AND ASSESSMENT OF IN VITRO ANTIOXIDANT EFFICACY OF EIGHT FERNS OF DARJEELING HIMALAYAS, INDIA

2021 ◽  
pp. 25-27
Author(s):  
Nishika Jaishee ◽  
Rohini Lama ◽  
Usha Chakraborty
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Phenolic Contents ◽  
Darjeeling Himalayas

AIM: The present study was performed to prole some phenolics and explore the antioxidant effect of eight locally available ferns collected from different places of Darjeeling Himalayas, India. Methods: The antioxidant activities of methanol (MeOH) extract was evaluated by DPPH free radical scavenging activity. Qualitative analysis of phenol was done using standard methods. Further, characterization of phenolics was done using High performance liquid chromatography. Result: The content of phenolics ranged from 6.77 to 60.066mg FAE/g dry weight. The DPPH antioxidant activity expressed as IC values 50 revealed Nephrolepis cordifolia and Microsorum punctatum to exhibit highest and lowest antioxidative activity respectively. Moderate correlation 2 (R =0.547) was observed between the total phenolics content and antioxidant activity. HPLC analysis of phenolics from all the investigated plants revealed the presence of caffeic acid, ferulic acid and salicylic acid while the other phenolics such as phloroglucinol, gallic acid, pyrogallol, 3,4- dihy droxybenzoic acid, catechol, catechin, chlorogenic acid, caffeine, vanillic acid and cinnamic acid were not uniformly present in all the plants. The phenolic contents values showed wide variation among themselves, as well as within different plants. These ferns with considerable amount of phenolics can be the potential source of natural antioxidants.

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