scholarly journals In Vitro Acaricidal Activity ofAtropa belladonnaand Its Components, Scopolamine and Atropine, againstRhipicephalus(Boophilus)microplus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
R. Godara ◽  
M. Katoch ◽  
R. Katoch ◽  
Anish Yadav ◽  
S. Parveen ◽  
...  
Keyword(s):  
Crude Extract ◽  
Mass Production ◽  
Methanolic Extract ◽  
Larval Mortality ◽  
Immersion Test ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Egg Mass ◽  
Atropa Belladonna

In vitro efficacy of methanolic extract ofAtropa belladonnaand its components scopolamine and atropine was assessed againstRhipicephalus(Boophilus)microplus. Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) were used whereas scopolamine and atropine were each tested at 0.1%. In adult immersion test, the extract was lethal to ticks at 20% concentration. The LC50 and LC95 values were determined as 6.875% and 17.306%, respectively. The extract caused a significant reduction (P<0.05) in egg mass production at 10% concentration. In larval packet test, the extract was lethal to larvae in the concentrations of 10% and 20% after 24 h, with LC50 and LC95 values of 1.321% and 4.935%, respectively. Scopolamine and atropine showed 93.3% and 60.0% mortality of adult ticks, respectively, but they caused complete (100%) blocking of hatching as well as 100% larval mortality. Scopolamine and atropine were observed to be more potent than the crude extract at an equivalent concentration in both the bioassays.

scholarly journals Acaricidal activity of Amburana cearensis on the cattle tick Rhipicephalus (Boophilus) microplus

2015 ◽  
Vol 46 (3) ◽  
pp. 536-541 ◽  
Author(s):  
Anne Caroline dos Santos Dantas ◽  
Andreina de Carvalho Araujo ◽  
Alessandra Gomes Marques Pacheco ◽  
Alexsandro Branco ◽  
Luis Antônio Sangioni ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Immersion Test ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Reproductive Efficiency ◽  
Egg Mass ◽  
Hatching Rate ◽  
Test Parameters ◽  
Gradual Dose

ABSTRACT: This experiment was carried out to study the bioacaricidal activity of Amburana cearensis against engorged females of Rhipicephalus (Boophilus) microplus. The crude ethanolic extract from the leaves of A. cearensis was submitted to partition with organic solvents (hexane, chloroform and ethyl acetate) in order of increasing polarity; and evaluated using three treatments at concentrations of 5, 10 and 25mg ml-1. To evaluate the acaricidal activity, engorged females were submitted to the adult immersion test. Parameters analyzed were the weight of females; weight of egg mass; percentage of hatch; reproductive efficiency; expected rate of reproduction; and efficacy. For each extract and concentrating fraction, tests were performed in triplicate. Among all fractions, the hexane (2.5%) achieved the best results in all parameters analyzed: 52.7% of inhibition of oviposition; 39% of hatching rate; 3,271 index of reproductive efficiency; and 67.0% effectiveness. The extract obtained gradual dose response with increasing concentration; and could be used as an aid in the control of R. (B.) microplus therapy.

scholarly journals In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
S. Parveen ◽  
R. Godara ◽  
R. Katoch ◽  
A. Yadav ◽  
P. K. Verma ◽  
...  
Keyword(s):  
Immersion Test ◽  
Rhipicephalus Microplus ◽  
Acaricidal Activity ◽  
Cattle Tick ◽  
Egg Mass ◽  
Ageratum Conyzoides ◽  
Artemisia Absinthium ◽  
Aerial Parts ◽  
Ethanolic Extracts

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

scholarly journals Acaricidal activity of Furcraea foetida leaf extract against engorged female Rhipicephalus (Boophilus) microplus ticks

2021 ◽  
Vol 37 ◽  
pp. e37031
Author(s):  
Henrique Aparecido de Sousa Martins ◽  
Maria de Fatima Pereira ◽  
Enéas Ricardo Konzen ◽  
Gilvano Ebling Brondani ◽  
Wellington Ferreira Campos
Keyword(s):  
Crude Extract ◽  
Leaf Extract ◽  
Skin Lesions ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Economic Damage ◽  
Attractive Alternative ◽  
Dependent Manner ◽  
Dose Dependent

The Rhipicephalus (Boophilus) microplus tick is a major concern for the livestock market worldwide, as it causes serious economic damage. Plant-derived acaricides are an attractive alternative to control this ectoparasite and limit the development of resistance. Therefore, the aim of this study was to evaluate the acaricidal activity of Furcraea foetida leaf extract against engorged female R. (B.) microplus ticks. Our in vitro bioassays showed that the crude extract of leaves from F. foetida caused hemorrhagic swelling and skin lesions in the ticks, and three days of treatment caused 100% mortality. Dose-response assay indicated that this toxicity effect was dose-dependent. Similar effects were observed when the crude extract from F. foetida leaves was denatured by boiling at 100°C. These results suggest that the toxicity of the leaf extract might be associated with thermostable biomolecules. Together, our results show for the first time that the crude extract of F. foetida leaves has acaricidal activity against engorged female R. (B.) microplus ticks and it acts in a dose-dependent manner.

scholarly journals Efficacy of extracts from plants of the Brazilian Pantanal against Rhipicephalus (Boophilus)microplus

2013 ◽  
Vol 22 (4) ◽  
pp. 532-538 ◽  
Author(s):  
Larissa Bezerra dos Santos ◽  
Juliana Kátia Souza ◽  
Barbara Papassoni ◽  
Dyego Gonçalves Lino Borges ◽  
Geraldo Alves Damasceno Junior ◽  
...  
Keyword(s):  
Immersion Test ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Stage I ◽  
Fruit Extract ◽  
Mato Grosso ◽  
Sesbania Virgata ◽  
Brazilian Pantanal ◽  
Mato Grosso Do Sul

This research evaluated the in vitro acaricidal activity of extracts from 21 plant species from the Pantanal of Mato Grosso do Sul. During stage I, a larval immersion test was performed using three extract concentrations (5%, 20%, and 40%). During stage II, we used only plants that showed over 95% efficiency at the 40% concentration in stage I in an amount sufficient for the adult immersion test. Aeschynomene denticulata, Angelonia hirta, Aspilia latissima, Caperonia castaneifolia, Centratherum punctatum, Crotalaria micans, Diodia kuntzei, Echinodorus paniculatus, Hyptis mutabilis, Lantana canescens, Melanthera latifolia, Ocotea diospyrifolia, Richardia grandiflora, Sebastiana hispida, Tocoyena formosa, Zanthoxylum rigidum, and Sesbania virgata (fruit extract) showed acaricidal activity against the larval stage ofRhipicephalus (Boophilus)microplus higher than 95% at a 40% (w/v) concentration, while Hippocratea volubilis and Randia armatashowed moderate efficacy and Croton glandulosus andSenna obtusifolia had no effect. The M. latifolia, A. hirta, R. grandiflora, and A. latissima raw extracts were evaluated for their activity against adults, and only A. hirta showed an efficacy close to 90%. Eighteen extracts had an efficacy of up to 95% against larvae at a 40% concentration, seven extracts were effective at 20%, and only one (Sebastiana hispida) was effective at a 5% concentration.

scholarly journals Evaluation of phytotherapy alternatives for controlling Rhipicephalus (Boophilus) microplus in vitro

2017 ◽  
Vol 26 (3) ◽  
pp. 299-306 ◽  
Author(s):  
José Pablo Villarreal Villarreal ◽  
Pedro Rassier dos Santos ◽  
Maria Antonieta Machado Pereira da Silva ◽  
Rosaria Helena Machado Azambuja ◽  
Carolina Lambrecht Gonçalves ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Sunflower Seed ◽  
Immersion Test ◽  
Tick Control ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Chemical Components ◽  
Brazil Nut ◽  
Acaricidal Efficacy

Abstract The objective of this study was to identify the main chemical components of the essential oil of Cuminum cyminum L. (cumin) and of the fixed oils of Bertholletia excelsa (Brazil nut) and of Helianthus annuus (sunflower seed). As well as testing the three oils and three different commercial synthetic acaricides against engorged females of Rhipicephalus (Boophilus) microplus in order to explore their acaricidal efficacy. Six different concentrations of the oils (200, 100, 50, 25, 12.5 and 6.25 mg/mL) and the active principles were evaluated with the Adult Immersion Test (AIT). The two main chemicals components of C. cyminum L. were the cuminaldehyde and the γ-terpinene. In both B. excelsa and H. annuus were the linoleic and oleic acid. C. cyminum L. showed high acaricidal activity (100%) over the engorged females and on their reproductive characteristat from the concentration of 100 mg/mL. B. excelsa and H. annuus had low acaricidal activity (39.39% and 58.75% in the concentration of 200 mg/mL respectively). The amidine and the pyrethroid (35.12% and 1.50% respectively). It can be concluded that the oil of C. cyminum L. may be a phytoterapic alternative for the cattle's tick control.

scholarly journals Control of bovine tick [Rhipicephalus (Boophilus) microplus] with Brunfelsia uniflora leaf extract

2019 ◽  
pp. 903-910
Author(s):  
Elaine Yae Yamashita Sugauara ◽  
ElisângelaYumi Sugauara ◽  
Rosangela Rumi Sugauara ◽  
Wanessa de Campos Bortolucci ◽  
Herika Line Marko de Oliveira ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Leaf Extract ◽  
Acid Ethyl Ester ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Ex Situ ◽  
In Vitro Tests ◽  
Free Living ◽  
Crude Leaf Extract

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. Brunfelsia uniflora is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. uniflora leaf ethanolic extract and its efficiency to control bovine tick in vitro and free-living stage ex situ. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in vitro against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in in vitro tests and was 100% efficient for ex situ larva test (free-living stage). B. uniflora leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.

scholarly journals In vitro efficacy of essential oils with different concentrations of 1,8-cineole against Rhipicephalus (Boophilus) microplus

2018 ◽  
Vol 27 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Karina Neoob de Carvalho Castro ◽  
Kirley Marques Canuto ◽  
Edy de Sousa Brito ◽  
Lívio Martins Costa-Júnior ◽  
Ivanilza Moreira de Andrade ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Probit Analysis ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization Detection ◽  
Flame Ionization ◽  
Alpinia Zerumbet

Abstract The aim of this study was to evaluate the acaricidal activity of essential oils from three species of plants with intermediary concentrations of 1,8-cineole against the tick species Rhipicephalus (Boophilus) microplus. For this purpose, five serial concentrations (100.0, 50.0, 25.0, 12.5, 6.2 mg/mL) of essential oils from Mesosphaerum suaveolens (L.) Kuntze, Ocimum gratissimum L. and Alpinia zerumbet (Pers.) B. L. Burtt & R. M. Sm. were used on larval packet and adult immersion tests. The essential oils were analysed by gas chromatography-mass spectrometry (GC/MS) and gas chromatography-flame ionization detection (GC-FID), being detected 35.8, 24.7 and 24.0% of 1.8-cineol in the oils of M. suaveolens, O. gratissimum and A. zerumbet, respectively. The lethal concentration (LC 50) of each oil for larvae and engorged females was calculated through Probit analysis. All essential oils showed high efficacy (≥ 95.0%) on engorged females at the 100.0 mg/mL concentration. In regards to larvae, O. gratissimum (LC 50 = 11.9 mg/mL) was the most potent, followed by the A. zerumbet (LC50 = 19.7 mg/mL) and the M. suaveolens (LC50 = 51.6 mg/mL) essential oils. These results show that other compounds interfere with 1,8-cineole action.

scholarly journals Efficiency of tick biotherapic on the control of infestation by Rhipicephalus (Boophilus) microplus in Dutch dairy cows.

2021 ◽  
Vol 9 (33) ◽  
pp. 156-164
Author(s):  
Zilda Cristiani Gazim ◽  
Fabiana Borges Padilha Ferreira ◽  
Aristeu Vieira Da Silva ◽  
Kelly Cristina Bolognese ◽  
Ewerton Merlin ◽  
...  
Keyword(s):  
Boophilus Microplus ◽  
Mineral Salt ◽  
Cattle Tick ◽  
Reproductive Efficiency ◽  
In Vitro Tests ◽  
Egg Mass ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Significant Difference

Background: cattle tick Rhipicephalus (Boophilus) microplus poses serious problems for farmers in Brazil, especially because the parasite easily develops resistance to pesticide agents. For this reason, together with other factors including environmental, human and animal contamination and costs, alternative approaches have been sought for. Aims: this study sough to evaluate the efficiency of a tick biotherapic on tick-infested cows. Methods: 34 dairy Dutch cows were divided in 2 groups: one group received 100g/day of mineral salt supplement impregnated with tick biotherapic 12cH for 6 months, and then in alternate days with tick biotherapic 30cH to complete 28 months of treatment; the other group (control) received only the mineral salt supplement. After 28 months of treatment, engorged Rhipicephalus (boophilus) microplus females were collected in both groups, counted and weighed; in vitro tests were carried out to assess mass of ticks; egg mass; egg-hatching rate; and reproductive efficiency. Results: There was significant difference between both groups for all parameters evaluated; tick-mass (p = 0.0008); egg mass (p=0.0044); egg-hatching rate (p= 0.0017); and reproductive efficiency (p = 0.0044). Conclusion: treatment with tick biotherapic significantly decreased the mass of engorged females, deposition and hatching rate of eggs, resulting consequently in the decrease of the reproductive efficiency of ticks.

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