scholarly journals Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGE/MS

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Sanja Kiprijanovska ◽  
Sotir Stavridis ◽  
Oliver Stankov ◽  
Selim Komina ◽  
Gordana Petrusevska ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
System Development ◽  
Proteome Analysis ◽  
Cellular Growth ◽  
Specific Information ◽  
Urogenital System ◽  
Urine Proteome ◽  
2D Page ◽  
Protein Components ◽  
Normal Urine

Proteome analysis of the urine has shown that urine contains disease-specific information for a variety of urogenital system disorders, including prostate cancer (PCa). The aim of this study was to determine the protein components of urine from PCa patients. Urine from 8 patients with clinically and histologically confirmed PCa was analyzed by conventional 2D PAGE. The MS identification of the most prominent 125 spots from the urine map revealed 45 distinct proteins. According to Gene Ontology, the identified proteins are involved in a variety of biological processes, majority of them are secreted (71%), and half of them are enzymes or transporters. Comparison with the normal urine proteome revealed 11 proteins distinctive for PCa. Using Ingenuity Pathways Analysis, we have found 3 proteins (E3 ubiquitin-protein ligase rififylin, tumor protein D52, and thymidine phosphorylase) associated with cellular growth and proliferation (p=8.35×10-4-3.41×10-2). The top network of functional associations between 11 proteins was Cell Death and Survival, Cell-To-Cell Signaling and Interaction, and System Development and Function (p=10-30). In summary, we have created an initial proteomic map of PCa patient’s urine. The results from this study provide some leads to understand the molecular bases of prostate cancer.

Reconstruction of associative protein networks connected with processes of sodium exchange regulation and sodium deposition in healthy volunteers based on urine proteome analysis

2012 ◽  
Vol 38 (3) ◽  
pp. 316-323 ◽  
Author(s):  
I. M. Larina ◽  
N. A. Kolchanov ◽  
I. V. Dobrokhotov ◽  
V. A. Ivanisenko ◽  
P. S. Demenkov ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Proteome Analysis ◽  
Protein Networks ◽  
Urine Proteome ◽  
Sodium Exchange

Insights into the physiological role of WT1 from studies of genetically modified mice

2004 ◽  
Vol 16 (3) ◽  
pp. 287-300 ◽  
Author(s):  
Maria Teresa Discenza ◽  
Jerry Pelletier
Keyword(s):  
Target Genes ◽  
Transcriptional Regulatory Network ◽  
System Development ◽  
Genetically Modified ◽  
Physiological Role ◽  
Protein Isoforms ◽  
Urogenital System ◽  
Protein Partners ◽  
Genetically Modified Mice

Discenza, Maria Teresa, and Jerry Pelletier. Insights into the physiological role of WT1 from studies of genetically modified mice. Physiol Genomics 16: 287-300, 2004; 10.1152/physiolgenomics.00164.2003.—The identification of WT1 gene mutations in children with WAGR and Denys-Drash syndromes pointed toward a role for WT1 in genitourinary system development. Biochemical analysis of the different WT1 protein isoforms showed that WT1 is a transcription factor and also has the ability to bind RNA. Analysis of WT1 complexes identified several target genes and protein partners capable of interacting with WT1. Some of these studies placed WT1, its downstream targets, and protein partners in a transcriptional regulatory network that controls urogenital system development. We review herein studies on WT1 knockout and transgenic models that have been instrumental in defining a physiological role for WT1 in normal and abnormal urogenital development.

scholarly journals O8A.4 Asbestos exposure and prostate cancer, really?

2019 ◽  
Vol 76 (Suppl 1) ◽  
pp. A71.1-A71
Author(s):  
Marie-Elise Parent ◽  
M Hugues Richard
Keyword(s):  
Prostate Cancer ◽  
Cancer Risk ◽  
Asbestos Exposure ◽  
Large Population ◽  
Prostate Cancer Risk ◽  
Cumulative Exposure ◽  
Population Exposure ◽  
Specific Information ◽  
Chrysotile Asbestos ◽  
Increased Risk

BackgroundGeneral population exposure to asbestos from residential insulation and from environmental sources during childhood have recently been associated with prostate cancer. While asbestos fibers can be found in the prostate of workplace-exposed men at autopsy, few occupational studies have reported on asbestos exposure and prostate cancer incidence. We examined the association between lifetime occupational exposure to chrysotile asbestos and prostate cancer risk in a large population-based case-control study.MethodsCases were 1933 men with histologically-confirmed incident prostate cancer, aged ≤75 years, diagnosed in 2005–2009 in Montreal. Concurrently, 1994 population controls from the same residential area and age distribution were randomly selected from electoral lists. In-person interviews elicited detailed socio-demographics, lifestyle and work histories. Industrial hygienists used job-specific information to provide semi-quantitative evaluations of intensity and frequency of exposure to 345 chemical agents, including asbestos, and a measure of confidence in the evaluation. Logistic regression was used to estimate odds ratios (OR) and 95% confidence intervals (CI) for prostate cancer risk associated with exposure to chrysotile asbestos.ResultsAfter restriction to probable and definite exposure, and application of a 5 year lag, 12.5% of cases and 11.8% of controls were ever exposed to asbestos (OR=1.1, 95% CI 0.9–1.3). For duration of exposure, there was no increase in risk of overall prostate cancer in the lower tertiles of exposure but risk was elevated in the upper tertile (OR=1.6, 95% CI 1.2–2.2). Similarly, for cumulative exposure, risk was elevated in the upper tertile only (OR=1.5, 95% CI 1.1–2.1). Analyses considering tumor grades also showed a higher risk in the upper tertile of cumulative exposure for non-aggressive (OR=1.5, 95% CI 1.1–2.2) and especially aggressive (OR=1.9, 95% CI 1.2–3.0) cancers.ConclusionOur findings are consistent with an increased risk of prostate cancer with prolonged and high cumulative exposure to chrysotile asbestos, and particularly for the aggressive form of the disease.

scholarly journals Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3

Sensors ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 139 ◽  
Author(s):  
Miguel Cabello ◽  
Haobo Ge ◽  
Carmen Aracil ◽  
Despina Moschou ◽  
Pedro Estrela ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Electrical Activity ◽  
Cell Model ◽  
Treatment Strategies ◽  
Electrode Array ◽  
Cellular Level ◽  
Low Noise ◽  
Cellular Growth ◽  
Male Population ◽  
Depth Analysis

Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatment strategies. Whilst electrophysiological studies can provide in depth analysis, the possibility of recording electrical activity in large populations of non-neuronal cells remains a significant challenge, even harder to address in the picoAmpere-range, which is typical of cellular level electrical activities. In this paper, we present the measurement and characterization of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA) with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with Gd3⁺, known as an inhibitor for the Ca2⁺ exchanges, suggest that the quasi-periodic signals originate from Ca2⁺ channels. After adding the Gd3⁺ to a population of living PC-3 cells, their electrical activity considerably decreased; once the culture was washed, thus eliminating the Gd3⁺ containing medium and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity. Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the use of Gd3⁺, on the timescale of this experiment. Hence, this experimental work suggests that Ca2⁺ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations. Our measuring platform opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways.

scholarly journals Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer

Oncotarget ◽  
2016 ◽  
Vol 8 (32) ◽  
pp. 52237-52255 ◽  
Author(s):  
Carolina Soekmadji ◽  
James D. Riches ◽  
Pamela J. Russell ◽  
Jayde E. Ruelcke ◽  
Stephen McPherson ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Extracellular Vesicles ◽  
Cellular Growth ◽  
Paracrine Signaling

scholarly journals Serum Levels of Selenium, Zinc, Copper, Manganese, and Iron in Prostate Cancer Patients

2020 ◽  
Vol 14 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Saleh A.K. Saleh ◽  
Heba M. Adly ◽  
Altaf A. Abdelkhaliq ◽  
Anmar M. Nassir
Keyword(s):  
Prostate Cancer ◽  
Trace Elements ◽  
Cancer Patients ◽  
Serum Levels ◽  
Cellular Growth ◽  
Control Groups ◽  
Icp Ms ◽  
Copper Manganese ◽  
Trace Element Contents ◽  
Serum Trace Elements

Backgrounds: Variations of trace element contents may be associated with several diseases including metabolic disorders, cellular growth disturbance, mutation and tumorigenesis. Prostate cancer is the second most common male cancer worldwide and stand fifth most common male cancer in Saudi Arabia. Objective: In the present study, Serum levels of selenium, zinc, copper, manganese, and iron were measured in patients with BPH and prostate cancer aiming to explore the association between these elements and prostate cancer. Patients and Methods: The study included 40 newly diagnosed prostate cancer patients, 22 patients with BPH and 30 healthy male subjects. All participant groups had similar socio-economic levels. Fasting blood samples were collected from all subjects and before any intervention for the patients. Serum PSA concentrations were analyzed by ELIZA and trace elements Se, Zn, Cu, Mn and Fe were measured by ICP-MS. Results: Serum Se, Zn, and Mn levels of prostate cancer patients were significantly decreased compared to control groups. The levels of serum Cu and Fe were significantly higher in prostate cancer patients than in control groups. Conclusion: In the present study, an association was noticed between serum trace elements disturbance and prostate cancer. The decreased levels of Se, Zn, and Mn, and increased Cu and Fe levels may play significant roles in the initiation of prostate cancer. However, future prospective studies on the causes of trace elements alteration in prostate cancer patients are needed as well as to illustrate the relation between different prostate cancer stages and trace elements concentrations.

Validated hemoglobin-depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS

2012 ◽  
Vol 33 (16) ◽  
pp. 2537-2545 ◽  
Author(s):  
Katja Walpurgis ◽  
Maxie Kohler ◽  
Andreas Thomas ◽  
Folker Wenzel ◽  
Hans Geyer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Proteome Analysis ◽  
2D Page ◽  
Cell Lysate ◽  
Orbitrap Ms

Toward a standardized urine proteome analysis methodology

2011 ◽  
Vol 5 (9-10) ◽  
pp. 547-547 ◽  
Author(s):  
Magali Court ◽  
Nathalie Selevsek ◽  
Mariette Matondo ◽  
Yves Allory ◽  
Jerome Garin ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
Urine Proteome ◽  
Analysis Methodology
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