scholarly journals Histological, Histochemical, and Protein Changes after Induced Malocclusion by Occlusion Alteration of Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Carolina de Souza Guerra ◽  
Yamba Carla Lara Pereira ◽  
João Paulo Mardegan Issa ◽  
Kelly Galisteu Luiz ◽  
Elaine A. Del Bel Guimarães ◽  
...  
Keyword(s):  
Masseter Muscle ◽  
Wistar Rats ◽  
Morphological Analysis ◽  
Contralateral Side ◽  
Environmental Interventions ◽  
Stomatognathic System ◽  
Masseter Muscles ◽  
Experimental Group ◽  
Muscle Remodeling

Although disorders of the stomatognathic system are common, the mechanisms involved are unknown. Our objective was to study the changes in the masseter muscles after unilateral exodontia. Molar extraction was performed on Wistar rats (left side), and the animals were sacrificed after either 14 or 26 days. The masseter muscle was processed for histological analysis, conventional andin situzymography, and immunohistochemistry. The morphological analysis showed unique and specific characteristics for the experimental group. By conventional zymography no significant values of 72 kDa MMP-2 (P< 0.05) were found in both of the sides of masseter muscle after 14 and 26 days of unilateral extraction. Thein situzymography showed gelatinolytic activity on all deep masseter muscles, with significant increase on the contralateral side after 14 and 26 days (P< 0.05). The immunohistochemistry demonstrated greater expression of MMP-2 than MMP-9 and MMP-14 in all masseter muscles and there were few differences in the staining of 4 TIMPs. This knowledge about morphology and molecular masticatory muscle remodeling following environmental interventions can be used to develop clinically successful treatments.

scholarly journals Functional Changes of Temporomandibular Joint Mechanoreceptors Induced by Reduced Masseter Muscle Activity in Growing Rats

2009 ◽  
Vol 79 (5) ◽  
pp. 978-983 ◽  
Author(s):  
Takayoshi Ishida ◽  
Tadachika Yabushita ◽  
Kunimichi Soma
Keyword(s):  
Temporomandibular Joint ◽  
Muscle Activity ◽  
Masseter Muscle ◽  
Growth Period ◽  
Control Group ◽  
Functional Changes ◽  
Male Wistar Rats ◽  
Masseter Muscles ◽  
Experimental Group ◽  
Indirect Stimulation

Abstract Objective: To determine the influence of masseter muscle activity during growth on the functional characteristics of temporomandibular joint (TMJ) mechanoreceptors. Materials and Methods: Sixty-six 3-week-old male Wistar rats were divided into an experimental group, in which the masseter muscles were bilaterally resected at 3 weeks of age, and a control group. Single-unit activities of the TMJ mechanoreceptors were evoked by indirect stimulation of passive jaw movement. Electrophysiologic recordings of TMJ units were made at 5, 7, and 9 weeks of age. Results: During this period, the firing threshold of the TMJ units was significantly lower and the maximum instantaneous frequency of the TMJ units was significantly higher in the experimental group than in the control group. Conclusion: Reduced masseter activity during the growth period alters the response properties of TMJ mechanoreceptors.

scholarly journals Morphometric analysis of the masseter muscle post unilateral lower molar exodonty: experimental study

2016 ◽  
Vol 33 (02) ◽  
pp. 090-095
Author(s):  
M. Benigno ◽  
E. Amstalden ◽  
E. Liberti ◽  
N. Leal ◽  
K. Rodrigues ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Masseter Muscle ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Optical Microscope ◽  
Morphometric Study ◽  
Surgical Extraction ◽  
Molar Teeth ◽  
Masseter Muscles ◽  
Lower Molar

Abstract Introduction: To check the morphological changes of the masseter muscle after surgical extraction of inferior molar teeth. Methods: 24 Wistar rats had samples of their masseter muscles analysed in the Optical Microscope (OM). The rats were randomly divided into three groups as follows: GI-15, GII-30, GII-60 days after surgical extraction of inferior molar teeth. Each group had 5 experimental and 3 normal control rats for OM observation. The OM allowed the morphometric study of the masseter muscle. The morphometric study was based on the measurement of the fiber's area by AXION-VISION software. ANOVA test was applied for data analysis. Results: No alteration was detected in all morphometric analysis of the masseter muscle. Conclusion: The masseter muscle adapts to the occlusal modiication caused by the extraction of the molar teeth during the analysed periods.

scholarly journals Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients

2020 ◽  
Vol 5 (4) ◽  
pp. 80
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Guglielmo Ramieri ◽  
Michele Tepedino ◽  
Michele Runci Anastasi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Contralateral Side ◽  
Surgical Patients ◽  
Posterior Crossbite ◽  
Fibre Formation ◽  
Masseter Muscles ◽  
Low Activity ◽  
Contralateral Muscle

A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place. Aim: the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients. Materials and methods: biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one. Conclusions: these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.

scholarly journals Masseter muscle surface electromyography in college students with a high degree of anxiety and temporomandibular disorder

Revista CEFAC ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Eduarda de Lima Amarante ◽  
Jully Anne Soares de Lima ◽  
Rafael Nóbrega Bandeira ◽  
Ana Paula Arruda de Moura ◽  
Luciane Spinelli de Figueiredo Pessoa ◽  
...  
Keyword(s):  
College Students ◽  
Electrical Activity ◽  
Masseter Muscle ◽  
Speech Therapy ◽  
Temporomandibular Disorder ◽  
P Value ◽  
Voluntary Activity ◽  
Group 2 ◽  
High Degree ◽  
Masseter Muscles

ABSTRACT Objective: to compare the electrical activity of masseter muscles, bilaterally, according to the presence or absence of Temporomandibular Disorder (TMD) in college students with a high degree of anxiety. Methods: the study was conducted with a randomized sample of 31 Speech Therapy students aged between 17 and 32 years; 61.3% (n = 19) were females and 38.7% (n = 12) were males. They were divided into two groups, Group 1 (G1), comprising 11 students with TMD, and Group 2 (G2), composed of 20 students without TMD. The college students answered the State-Trait Anxiety Inventory (STAI) for anxiety investigation, and were evaluated by the protocol Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) for TMD diagnosis. The evaluation of muscular electrical activity took into account the records in the conditions of rest, Sustained Maximum Voluntary Activity (SMVA) and habitual chewing (HC). The data were analyzed using the version 22 IBM Statistical Package for Social Sciences (SPSS) software. The statistical analysis was performed using Student t test to compare means between groups, considering < 0,05 as the significant p-value. Results: college students, of both groups, presented high levels of anxiety traits. Significant statistical differences were observed on the percentage of electrical activity of right masseter muscle in chewing function, as well as muscle fibers recruitment during chewing, which were higher on the group without TMD. Conclusion: college students with TMD and a high degree of anxiety presented lower means of masseter muscle electromyografic activity during chewing, in most conditions assessed, as compared to volunteers without TMD, except for the left masseter muscle in rest and chewing.

scholarly journals Stiffness of the Masseter Muscle in Children—Establishing the Reference Values in the Pediatric Population Using Shear-Wave Elastography

2021 ◽  
Vol 18 (18) ◽  
pp. 9619
Author(s):  
Cyprian Olchowy ◽  
Anna Olchowy ◽  
Aleksander Pawluś ◽  
Mieszko Więckiewicz ◽  
Luca Maria Sconfienza
Keyword(s):  
Shear Wave ◽  
Masseter Muscle ◽  
Pediatric Population ◽  
Shear Wave Elastography ◽  
Total Sample ◽  
Healthy Children ◽  
Significant Difference ◽  
The Right ◽  
The Impact ◽  
Masseter Muscles

In children, the quality and muscle function are altered in many pathologic conditions, including temporomandibular disorders. Although several methods have been used to evaluate muscle tonus, none became a golden standard. Moreover, the masseter muscle characteristics in children have not been investigated to date. This study aimed to measure the stiffness of the masseter muscle using shear-wave elastography in healthy children. We enrolled 30 healthy children (mean age 10.87 ± 3.38 years). The stiffness of masseter muscles was measured with shear wave elastography. Stiffness for the total sample was 6.37 ± 0.77 kPa. A comparison of the measurements did not show significant differences between the right and the left masseter muscles (left—6.47 ± 0.78 kPa; right—6.24 ± 0.76 kPa; p = 0.3546). A significant difference was seen between boys and girls (boys—5.94 ± 0.50 kPa; girls—6.63 ± 0.80; p = 0.0006). Shear-wave elastography is a promising diagnostic tool. It may help to detect changes in the stiffness of the masseter muscle and draw attention to pathological processes within the jaw muscles. Directions for further research shall include determining stiffness values in pathological conditions and the impact of biological and functional factors on the stiffness of the masseter muscle.

scholarly journals Using different iodine staining for morphological analysis from microCT images in muscle structure of wistar rats

2019 ◽  
Author(s):  
Fábio Piau ◽  
◽  
Alexandre Freire ◽  
Juliana Haddad ◽  
Ana Rossi ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Morphological Analysis ◽  
Muscle Structure ◽  
Iodine Staining

scholarly journals Efficacy of Immobilized Prolonged Ammonium Antiseptics in Treatment of Purulent Wounds

2017 ◽  
Vol 10 (4) ◽  
pp. 269-276
Author(s):  
B S Sukovatykh ◽  
A Y Grigoryan ◽  
A I Bezhin ◽  
T A Pankrusheva ◽  
Yu Yu Blinkov ◽  
...  
Keyword(s):  
Sodium Salt ◽  
Group Treatment ◽  
Benzalkonium Chloride ◽  
Wistar Rats ◽  
Microbial Contamination ◽  
Comparison Group ◽  
Wound Area ◽  
Purulent Wounds ◽  
Male Wistar Rats ◽  
Experimental Group

Relevance. Purulent complications occur in 35-45% of patients with surgical diseases, lethality rate from these conditions achieves 25%. The purpose of the study. To investigate the wound process while treating the purulent wounds with immobilized forms of miramistin and benzalkonium chloride in gel of sodium salt of carboxymetylcellulose. Materials and methods. There were analysed results of experimental study of the wound process on 108 male Wistar rats. Experimental animals were divided into 3 groups, each group included 36 rats. In a comparison group ‘Levomekol’ ointment was used for treatment. In the first experimental group treatment was done by the ointment with the following composition: 0,01% solution of miramistin — 100 g, metronidazol –1,0 g, sodium salt of carboxymetylcellulose — 4,0 g, while in the second experimental group ointment composition included benzalkonium chloride 0,02 g, metronidazol — 1,0 g, sodium salt of carboxymetylcellulose — 4,0 g, purified water — up to 100,0 g. The wound process was evaluated by planimetric, microbiological and histological methods of investigation. Results and its discussion. The reduction of wound area in the first experimental group was higher than in a comparison group on the 3rd day of the experiment by 9,7%, on the 5th day — by 7,6%, on the 10th and 15th days — by 10,5%, while in the second experimental group the change was 37,2%, 27,3%, 13,8% and 6,9% respectively. In the first experimental group microbial contamination of the wound was less than in a comparison group by 5,8х106, on the 5th day — by 3,1х105, on the 10th day — by 6,1х104, while in the second experimental group on the 3rd day there were no differences, on the 5th day — by 6,3х105, on the 10th day — 1,1х104. Histological investigations of the wound biopsies on the 10th day of experiment showed that in a comparison group the bottom of the wound was covered by granulation tissue without signs of epithelization, there was full coverage of the wound with a newly formed epithelium in experimental groups. Conclusions. The use of prolonged quaternary ammonium antiseptics in treatment of purulent wounds is pathogenetically justified and efficient.

scholarly journals Effect of triiodothyronine on the maxilla and masseter muscles of the rat stomatognathic system

2011 ◽  
Vol 44 (7) ◽  
pp. 694-699
Author(s):  
M.V. Mariúba ◽  
F. Goulart-Silva ◽  
S. Bordin ◽  
M.T. Nunes
Keyword(s):  
Stomatognathic System ◽  
Masseter Muscles

scholarly journals Increased vertical dimension effects on masseter muscle fiber phenotype during maturation

2012 ◽  
Vol 83 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Charles Widmer ◽  
Vo Danh Nguyen ◽  
Harmeet Chiang ◽  
Joyce Morris-Wiman
Keyword(s):  
Muscle Fiber ◽  
Masseter Muscle ◽  
Fiber Diameter ◽  
Vertical Dimension ◽  
Control Group ◽  
Maxillary Molars ◽  
Fiber Size ◽  
And Control ◽  
Masseter Muscles ◽  
Anterior Posterior

Abstract Objective: To determine changes in mouse myosin heavy chain (MyHC) protein expression that may occur with a clinically relevant vertical dimension of occlusion (VDO) increase. Materials and Methods: Six CD-1 male mice (age: 6 weeks) underwent a 10% bite opening to replicate the clinical condition using composite on the maxillary molars and were compared to six age-matched controls. Mice were sacrificed at day 7 and 14 after bite opening. A representative masseter transverse cryosection from each animal was examined in selected sampling regions (anterior, posterior, posterior-deep, and posterior-intermediate) to assay fiber phenotype proportions and fiber size. Results: In control masseter muscles, the proportion of muscle fibers containing MyHC IIb increased in the posterior-intermediate and posterior-deep regions between 7 and 14 days (ANOVA, P &lt; .05). The increase in the proportion of MyHC IIb fibers in the bite opening group did not occur when compared to the control group (P &lt; .05). In addition, after 14 days of bite opening, the proportion of fibers positive for MyHC IIa was decreased in the anterior region compared to control masseter muscles. Muscle fiber diameter remained unchanged in both groups (experimental and control) and over time (P &gt; .10). Conclusion: These data are consistent with a selective plasticity of the expression of MyHC IIb protein in the deep regions of the male masseter muscle in response to a clinically relevant VDO increase.

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