scholarly journals In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
A. Z. Yildirim-Bicer ◽  
I. Peker ◽  
G. Akca ◽  
I. Celik

Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials.Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups(n=10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions ofCandida albicansATCC#90028 andCandida albicansoral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn’s test were used for multiple comparisons. Mann WhitneyUtest was used to compare the surface roughness.Results. Statistically significant difference(P<0.05)was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

Author(s):  
Saeed Noorollahian ◽  
Farinaz Shirban ◽  
Vahid Mojiri

Introduction: The daily use of orthodontic removable plates can interfere with the self-cleansing function of the mouth. Although various techniques have been proposed for cleaning removable orthodontic appliances, there is no consensus on the use of a safe method that preserves the physical properties of the appliance. This study aimed to investigate the effect of using hydrochloric acid (10%, for removal of mineral deposits) and sodium hypochlorite (5.25%, to remove organic matter and discolorations) on surface hardness and roughness of self-cure orthodontic acrylic resins. Materials & Methods: This cross-sectional laboratory study was conducted in the fall of 2017 at Isfahan University of Medical science and Isfahan University of Technology. In this study two orthodontic acrylic resins (Orthocryl® and Acropars®) were used. Eighty samples (12×10×3 mm) from each one were fabricated and divided into four groups (n = 20). Group 1: 15 minutes immersion in household cleaner liquid (Hydrochloric acid, 10%) followed by15 minutes immersion in household bleach liquid (Sodium Hypochlorite, 5.25%). In group 2, immersions were repeated just like group two times and in group 3, were done three times. Group 4 was as control and had no immersion. The surface hardness and roughness of samples were measured. Data were analyzed with Two Way ANOVA and the significance level was set at 0.05. Results: The number of immersion procedures did not significantly affect the surface hardness (p value = 0.958) and surface roughness (p value = 0.657) in the different study groups. There was no significant difference in the surface hardness between the two acrylic resin brands (p value = 0.077); however, Acropars acrylic resin samples exhibited significantly higher surface roughness compared to the Orthocryl acrylic resin samples(p value < 0.001). Conclusion: 15 minutes of immersion in 10% HCl, followed by 15 minutes of immersion in 5.25% NaOCl and repetition of the procedure three times did not significantly affect the surface hardness and roughness of self-cured acrylic resins.


2010 ◽  
Vol 55 (10) ◽  
pp. 763-770 ◽  
Author(s):  
Camila Andrade Zamperini ◽  
Ana Lucia Machado ◽  
Carlos Eduardo Vergani ◽  
Ana Claudia Pavarina ◽  
Eunice Terezinha Giampaolo ◽  
...  

2018 ◽  
Vol 21 (1) ◽  
pp. 96 ◽  
Author(s):  
Tabata Prado Sato ◽  
Celio Ilidio Conjo ◽  
Rodnei Dennis Rossoni ◽  
Juliana Campos Junqueira ◽  
Renata Marques de Melo ◽  
...  

<p><strong>Objective: </strong>This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from <em>Fusarium oxysporum</em> on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against <em>Candida albicans</em>. <strong>Material and Methods:</strong> For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated <em>in vitro</em> with regard to <em>C. albicans</em> biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. <strong>Results:<em> </em></strong>The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. <strong>Conclusion:</strong> It can be concluded tha AgNPs incorporation in HAAR was effective in reducing <em>C. albicans</em> activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.</p><p><strong>Keywords</strong></p><p>Acrylic resins; Silver; Antimicrobial agents.</p>


2017 ◽  
Vol 65 (4) ◽  
pp. 303-307
Author(s):  
Gabriel Lúcio Calazans DUARTE ◽  
Angélica Kercya Pereira de MENDONÇA ◽  
Ana Roberta Assunção de FREITAS ◽  
Antônio Ricardo Calazans DUARTE ◽  
Julita de Campos Pipolo HOLANDA

ABSTRACT Objective : To evaluate the surface roughness of two makes of autopolymerized acrylic resin (Classic Dencor® and Duralay®) with two different methods of finishing and polishing (conventional and using the Dhpro® finishing & polishing kit). Methods : A total of 20 specimens (10 of each make) was obtained using Zetalabor®--Zhermak condensation silicone molds. After polymerization, 20 blocks of resin were divided in two and subjected to two types of finishing and polishing, one at each end. The types of finishing and polishing were as follows: conventional (lathe) and using the Dhpro® finishing & polishing kit. Once finished and polished, the specimens were subjected to surface roughness testing using a roughness meter and were analyzed via the Student’s t-test. Results : There is a statistically significant difference between the forms of polishing, unrelated to the brand of acrylic resin. Conventional polishing achieved a level of 0.12 µm for both resins and the DhPro® polishing presented roughness a little above 0.2 µm. Conclusion : Conventional polishing is superior to the DhPro® kit, as it achieves lower levels of roughness. There is no significant difference between the acrylic resins in terms of surface roughness after the finishing and polishing processes.


Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 221
Author(s):  
Avi Meirowitz ◽  
Arkadi Rahmanov ◽  
Eti Shlomo ◽  
Helena Zelikman ◽  
Eran Dolev ◽  
...  

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.


2017 ◽  
Vol 18 (8) ◽  
pp. 679-682
Author(s):  
Nitin Gautam ◽  
Aditi Sharma ◽  
Unjum Bashir ◽  
Sisir Rapolu ◽  
Nallamilli Mamatha ◽  
...  

ABSTRACT Aim This study was undertaken to study the effect of three most commonly used dentifrices for denture cleaning and to find out any positive association between number of strokes by toothbrush and surface roughness of acrylic resin. Materials and methods About 40 acrylic rectangular blocks with high finish were polished until surface roughness was calculated as 0 µm. The materials tested were distilled water as a control group, Patanjali Dant Kanti, Colgate Total Cream, and Cleansodent as dentifrices. Stroking was done with dentifrices, and surface roughness readings were recorded at 1,000, 9,000, and 18,000 strokes. Results Patanjali Dant Kanti was found to have maximum abrasive potential followed by Colgate Total Cream, followed by Cleansodent and least by distilled water. Conclusion Surface roughness was found to be directly proportional to the type, size, and fineness of the abrasive component used; the professional denture cleanser (Cleansodent) has less abrasive potential than the regular toothpastes. Among those tested, the highest abrasive potential was found in Patanjali Dant Kanti and lowest by Cleansodent (denture cleanser). Clinical significance The patient as well as dentist should choose the dentifrice with utmost care noting the size and type of abrasives used. Washing the dentures normally with water without toothbrush daily and cleaning of dentures once in 10 to 15 days with a soft toothbrush and professional denture cleanser should be the line of maintenance of dentures. How to cite this article Gautam N, Sharma A, Bashir U, Rapolu S, Mamatha N, Sravani S. Effect of Different Dentifrices on the Surface Roughness of Acrylic Resins: An in vitro Study. J Contemp Dent Pract 2017;18(8):679-682.


2017 ◽  
Vol 19 (1) ◽  
Author(s):  
Juán José Castillo ◽  
Santiago Herrera ◽  
Pablo Andrés Rey ◽  
Carlos Mejía Pavony ◽  
Adriana Jaramillo

SUMMARYObjective: The purpose of this in vitro studywas to compare the tensile strength betweentwo materials for the manufacture ofacrylic denture bases (Veracril® from NewStetic and SR Triplex Hot® from IvoclarVivadent), and to know the mechanicalproperties of materials to study that haveclinical implications.Methods: Laboratory tests were conductedunder the technical standard of ASTMInternational # D 638-03 to evaluate thetwo acrylic resin materials for denturebases, processed by pressed moulding. Theobservations were made using the universaltesting machine Tinius Olsen® brandH50KS to analyze the tensile propertiessuch as modulus of elasticity, elongation,yield strength, maximum strength, energy,effort and tensile stress between the twomaterials evaluated.Results: For the effort, maximum strengthand modulus of elasticity, high-impactacrylic resin SR Triplex Hot® showedhigher values. With regard to the characteristicsof maximum displacementbefore the fracture, elongation and energy,conventional acrylic resin showed highervalues. The only characteristic that showeda significant difference between the twogroups of thermo-cured acrylic resin wasthe modulus of elasticity where the highimpactacrylic resin obtained a much highervalue than conventional acrylic resin.Conclusions: The acrylic resin SR TriplexHot® presents higher values in some tensileproperties, while the Veracril® showsbetter in others. The only characteristic thatshowed a significant difference betweenthe two acrylic resins was the modulus ofelasticity.Keywords: Acrylic resin denture bases,tensile strength, high impact acrylic resin.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Reem Abualsaud ◽  
Doaa M. Aleraky ◽  
Sultan Akhtar ◽  
Soban Q. Khan ◽  
Mohammed M. Gad

Objective. To evaluate the antimicrobial effects of different concentrations of zirconium dioxide nanoparticles (nano-ZrO2) reinforcement of poly(methyl) methacrylate (PMMA) on surface roughness and C. albicans biofilm. Methods. 20 heat-polymerized acrylic resin discs were conventionally made and divided into 4 groups (n = 5) according to nano-ZrO2 concentration: control (0% filler) and 3 experimental groups (2.5% (Z2.5), 5.0% (Z5.0), and 7.5% (Z7.5)). An optical profilometer was used for surface roughness evaluation, followed by Candida adherence assay. Specimens were sterilized, then immersed in cultured yeast (C. albicans), and incubated at 37°C for 48 hours. After that, discs were rinsed before extracting the clustered pellets of Candida. The attached C. albicans was counted using the direct method after spreading on agar media and incubating for 48 hours. Statistical analysis was performed using one-way ANOVA and Tukey’s post hoc test at α = 0.05. Results. Surface roughness was significantly increased with all modified groups compared with control ( P < 0.01 ), which showed the lowest roughness value (0.027 ± 0.004 μm). There was no significant difference in the roughness value among reinforced groups (2.5, 5.0, and 7.5%) ( P > 0.05 ), with Z7.5 showing the highest roughness value (0.042 ± 0.004 μm). Candida count was reduced as the nano-ZrO2 increased but not significantly ( P = 0.15 ). Conclusions. The addition of different concentrations of nano-ZrO2 particles to PMMA increased the surface roughness compared with control; in contrast, insignificant reduction of C. albicans biofilm was detected.


2007 ◽  
Vol 8 (5) ◽  
pp. 18-25 ◽  
Author(s):  
Banu Filiz Tari ◽  
Dilek Nalbant ◽  
Funda Dogruman Al ◽  
Semra Kustimur

Abstract Aim Candida albicans (C. albicans) has been widely associated with the etiology of denture-related stomatitis and has been found on soft denture lining materials. The aim of this study was to examine the surface roughness and adherence of C. albicans to saliva coated and non-coated soft lining materials by subjecting them to an in vitro accelerated aging test. Methods and Materials Samples were prepared from three soft lining materials (Visco Gel, Ufi Gel P, Molloplast B). Surface roughness measurements and adhesion of C. albicans were examined before and after an aging process. The stimulated human whole saliva was used to assess its effect on adhesion. Results The aging process promotes the surface roughness of soft lining materials. The aging surface roughness of Visco Gel was significantly higher than Ufi Gel P and Molloplast B. No significant difference was observed between non-aged and uncoated materials, but aged and uncoated soft lining materials showed a greater adherence of C. albicans. No significant difference was observed between non-aged and saliva coated materials, but aged and saliva coated soft lining materials showed a greater adherence of C. albicans. Conclusions Candidosis induced by C. albicans is the most common fungal infection. Awareness of susceptibility of soft lining materials to the adherence of C. albicans is an important factor in their selection. The use of soft lining materials with smooth surfaces minimizes the adherence of C. albicans. Citation Tari BF, Nalbant D, Dogruman Al F, Kustimur S. Surface Roughness and Adherence of Candida Albicans on Soft Lining Materials as Influenced by Accelerated Aging. J Contemp Dent Pract 2007 July;(8)5:018-025.


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