scholarly journals Antifungal Activity of Denture Base Resin Containing Nanozirconia: In Vitro Assessment of Candida albicans Biofilm

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Reem Abualsaud ◽  
Doaa M. Aleraky ◽  
Sultan Akhtar ◽  
Soban Q. Khan ◽  
Mohammed M. Gad
Keyword(s):  
Surface Roughness ◽  
Direct Method ◽  
Acrylic Resin ◽  
Denture Base ◽  
Significant Difference ◽  
Base Resin ◽  
Optical Profilometer ◽  
Agar Media ◽  
Post Hoc

Objective. To evaluate the antimicrobial effects of different concentrations of zirconium dioxide nanoparticles (nano-ZrO2) reinforcement of poly(methyl) methacrylate (PMMA) on surface roughness and C. albicans biofilm. Methods. 20 heat-polymerized acrylic resin discs were conventionally made and divided into 4 groups (n = 5) according to nano-ZrO2 concentration: control (0% filler) and 3 experimental groups (2.5% (Z2.5), 5.0% (Z5.0), and 7.5% (Z7.5)). An optical profilometer was used for surface roughness evaluation, followed by Candida adherence assay. Specimens were sterilized, then immersed in cultured yeast (C. albicans), and incubated at 37°C for 48 hours. After that, discs were rinsed before extracting the clustered pellets of Candida. The attached C. albicans was counted using the direct method after spreading on agar media and incubating for 48 hours. Statistical analysis was performed using one-way ANOVA and Tukey’s post hoc test at α = 0.05. Results. Surface roughness was significantly increased with all modified groups compared with control ( P < 0.01 ), which showed the lowest roughness value (0.027 ± 0.004 μm). There was no significant difference in the roughness value among reinforced groups (2.5, 5.0, and 7.5%) ( P > 0.05 ), with Z7.5 showing the highest roughness value (0.042 ± 0.004 μm). Candida count was reduced as the nano-ZrO2 increased but not significantly ( P = 0.15 ). Conclusions. The addition of different concentrations of nano-ZrO2 particles to PMMA increased the surface roughness compared with control; in contrast, insignificant reduction of C. albicans biofilm was detected.

IMMERSION OF HEAT POLYMERIZED ACRYLIC RESIN DENTAL BASE IN ARAK BALI AGAINST SURFACE ROUGHNESS

2021 ◽  
Vol 17 (1) ◽  
pp. 22-26
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
I Made Hendri Dwi Saputra ◽  
Keyword(s):  
Surface Roughness ◽  
Acrylic Resin ◽  
Control Group ◽  
Surface Strength ◽  
Denture Base ◽  
A Value ◽  
Removable Denture ◽  
Significant Difference ◽  
Post Test ◽  
Oral Tissue

Introduction: The denture base is the part of the removable denture that is supported by good adaptation to the underlying oral tissue. Most of the denture bases are made of acrylic or polymethyl methacrylate resin, better known as PMMA. However, the acrylic resin also has disadvantages such as easily broken and absorbs liquids both water and chemicals. The chemical absorption like alcohol, ethanol, and some drinks that contain acidic materials will chemically be induced with acrylic resin and settle in the pores of the acrylic resin. The chemical damage or defect creates roughness on the surface of the acrylic resin which can cause cracking or crazing and a decrease in surface strength and hardness. Materials and Methods: The method used in this research was a laboratory experimental design with a post-test-only control group using 12 samples consisting of 2 different types of samples by measuring the surface roughness of the acrylic resin after immersing the sample with a predetermined time. Results and Discussions: One-way ANOVA test results showed a significant difference in surface roughness after the samples immersion with a value of p = 0.006 (p <0.05). Conclusions: Based on this research, can be concluded that there is an increase in the surface roughness of the heated polymerized acrylic resin for 3 hours and 4 hours of immersion. The longer the heated polymerized acrylic resin is soaked in arak hence the level of surface roughness increases.

scholarly journals Effect of Denture Base Reinforcement Using Light Cured E- Glass Fibers on the Level of Salivary Immunoglobulin A

2018 ◽  
Vol 6 (11) ◽  
pp. 2168-2172
Author(s):  
Shady M. El Naggar ◽  
Mohamed I. Seif El Nasr ◽  
Hassan M. Sakr ◽  
Sherihan M. Eissa ◽  
Asmaa N. Elboraey ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Acrylic Resin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Complete Dentures ◽  
Denture Base ◽  
Mean Values ◽  
Glass Fibres ◽  
Significant Difference ◽  
Salivary Immunoglobulin A

BACKGROUND: A gap still exists between in vitro and clinical studies concerning the biocompatibility of the material in the oral environment and their potential to cause immunological undesirable side effects. The uses of glass fibres to improve the mechanical properties of acrylic resin denture base polymers are well documented in vitro. AIM: The present study aimed to evaluate the effect of denture base reinforcement using light-cured E- glass fibres mesh on the level of salivary immunoglobulin A (S-IgA) in patients wearing complete dentures. MATERIAL AND METHODS: Fourteen completely edentulous patients, in need of complete dentures, participated in the study. The patients were divided into two groups (n = 7) according to the treatment protocol. In the first group, patients received conventional heat-cured acrylic resin dentures. In the second group, the mandibular dentures were reinforced using light cured resin impregnated E glass fibres mesh. In both groups, salivary samples were collected using passive drool technique. The level IgA was assessed by enzyme-linked immunosorbent assay (ELISA) technique at different time intervals. Statistical analysis was carried out using one-way ANOVA followed by Tukey`s post-hoc test and independent t-test. The significant level was set at P ≤ 0.05. RESULTS: Acrylic resin dentures and reinforced ones demonstrated an increase in the mean values of IgA level at the end of the follow-up intervals. And this increase was statistically significant (P ≤ 0.05). Although, the reinforced dentures revealed higher mean values, there was no statistically significant difference between the two groups (P > 0.05) CONCLUSIONS: Within the limitations of the present study, the following could be concluded: (1) the insertion of complete dentures induced changes in the level of IgA; and (2) denture base reinforcement using light cured resin impregnated E-glass fibres mesh had a similar effect to that of heat cured acrylic resin on the level of IgA.

scholarly journals Effect of Different Denture Base Cleansers on Surface Roughness of Heat Polymerised Acrylic Materials with Different Curing Process

2020 ◽  
pp. 281-289
Author(s):  
Kubra Degirmenci ◽  
Mustafa Hayati Atala ◽  
Canan Sabak
Keyword(s):  
Surface Roughness ◽  
Base Material ◽  
Chlorhexidine Gluconate ◽  
Distilled Water ◽  
Short Term ◽  
Denture Base ◽  
Cleaning Agents ◽  
Significant Difference ◽  
Post Hoc

Avoiding biofilm formation on dentures is associated with maintaining the surface properties of acrylic-based dentures. The aim of the study is to investigate the effects of two different cleaning agents (Corega and Klorhex 0.2% chlorhexidine gluconate) on surface roughness of the denture regarding efficacy of curing procedures. A total of sixty disc-shaped specimens were prepared with two different methods as long and short curing processes. The specimens were divided into subgroups according to the immersion solutions (distilled water, Corega tablet group and Klorhex 0.2% chlorhexidine gluconate group) (n=10). The samples were kept in a solution for 8 hours per day during a month. The average Ra1 (before exposure to the cleanser agent) and Ra2 (after exposure to the cleanser agent) of each sample was measured. A two-way ANOVA and post hoc Tukey test was used for statistical analysis. The solutions significantly increased the Ra values in both acrylic groups (p<0.001). While the effect of the distilled water group was significantly lower than Corega and Klorhex in the long-term curing group (p<0.05), no significant difference was found in the short-term curing group (p>0.05). The long-term curing time is highly effective in decreasing the surface roughness of the acrylic base material.

scholarly journals The effect of Zirconium Dioxide nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells (in vitro study)

2021 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Rudy S ◽  
Titik Ismiyati ◽  
Endang Wahyuningtyas
Keyword(s):  
Cell Viability ◽  
Zirconium Dioxide ◽  
Acrylic Resin ◽  
Zro2 Nanoparticles ◽  
Fibroblast Cells ◽  
Denture Base ◽  
Group I ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Heat cured acrylic resin is the most commonly used denture base materials.  Zirconium dioxide (ZrO2) nanoparticles can be applied as additional filler to increase mechanical strength and to reduce the amount of residual monomer. The aim of this research is to analyze the effect of ZrO2 nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells. Twenty four disc-shaped heat cured acrylic resin plate (diameter 5 mm; width 2 mm), were divided into 4 groups (n=6), they were consisted of group I control (acrylic resin), group II acrylic resin with 2.5% ZrO2, group III acrylic resin with 5% ZrO2, and group IV acrylic resin with 7.5% ZrO2. Cell viability was obtained using MTT assay and ELISA plate reader. The result is examined with one way ANOVA followed by LSD post hoc assessment. The result showed highest cell viability percentage on experimental group of 2.5% ZrO2 with value as high as 97.49%. One way ANOVA test and LSD post hoc test showed a significant difference between groups (p<0.05). The conclusion of this research is ZrO2 nanoparticles concentration utilized as filler on heat cured acrylic resin denture base is effect to viability of fibroblast cells, and ZrO2 nanoparticles 2.5% shows the highest viability of fibroblast cell compared to 5% and 7.5% ZrO2 nanoparticles concentrations  

scholarly journals Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

2018 ◽  
Vol 21 (1) ◽  
pp. 96 ◽  
Author(s):  
Tabata Prado Sato ◽  
Celio Ilidio Conjo ◽  
Rodnei Dennis Rossoni ◽  
Juliana Campos Junqueira ◽  
Renata Marques de Melo ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Fusarium Oxysporum ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Acrylic Resin ◽  
Control Group ◽  
Significant Difference ◽  
Color Alteration ◽  
Cie Lab

<p><strong>Objective: </strong>This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from <em>Fusarium oxysporum</em> on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against <em>Candida albicans</em>. <strong>Material and Methods:</strong> For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated <em>in vitro</em> with regard to <em>C. albicans</em> biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. <strong>Results:<em> </em></strong>The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. <strong>Conclusion:</strong> It can be concluded tha AgNPs incorporation in HAAR was effective in reducing <em>C. albicans</em> activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.</p><p><strong>Keywords</strong></p><p>Acrylic resins; Silver; Antimicrobial agents.</p>

scholarly journals Influence of adhesive and bonding material on the bond strength of bracket to bovine tooth

2017 ◽  
Vol 16 ◽  
pp. 1-7
Author(s):  
Bruna Lorena dos Santos Oliveira ◽  
Ana Rosa Costa ◽  
Américo Bortolazzo Correr ◽  
Marcus Vinicius Crepaldi ◽  
Lourenço Correr-Sobrinho ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Acrylic Resin ◽  
Single Bond ◽  
Universal Adhesive ◽  
Bonding Material ◽  
Significant Difference ◽  
Post Hoc ◽  
Group 1

Aim: To evaluate, in vitro, the effect of adhesive and bonding materials on the shear bond strength (SBS) of metallic brackets bonded to bovine teeth. Methods: Bovine teeth were embedded with acrylic resin, etched with 35% phosphoric acid for 20 s, rinsed, dried for 20 s and divided into 8 groups (n=20), according to the bonding materials and adhesive: Groups 1 and 5 – Transbond XT; Groups 2 and 6 – Fill Magic; Groups 3 and 7 – Biofix; and, Groups 4 and 8 – Orthocem. One layer of adhesive 3 M Unitek (Group 1), and one layer of Single Bond Universal Adhesive (Groups 2, 3 and 4) were applied and light-cured with LED for 10 s. Brackets were bonded to the buccal surface with four bonding materials and light-cured with LED for 40 s. SBS was carried out after 24 h and thermocycling (7,000 cycles - 5°/55 °C). Data were submitted to two way ANOVA and Tukey’s post hoc test (α=0.05). The Adhesive Remaining Index (ARI) was evaluated at 8×. Results: The adhesive was effective in increasing the SBS for all bonding materials (p<0.05). Significant difference (p<0.05) on the SBS was observed between bonding materials with or without adhesive. The ARI showed a predominance of scores 0 for all groups. Conclusions: The adhesive improved significantly the SBS of the brackets to teeth. Different SBS values occurred among the bonding materials. ARI index showed predominance of score 0 for all groups.

scholarly journals The Reinforcement Effect of Nano-Zirconia on the Transverse Strength of Repaired Acrylic Denture Base

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed Gad ◽  
Aws S. ArRejaie ◽  
Mohamed Saber Abdel-Halim ◽  
Ahmed Rahoma
Keyword(s):  
Glass Fiber ◽  
Acrylic Resin ◽  
Bending Test ◽  
Repeated Measure ◽  
Denture Base ◽  
Intact Group ◽  
Transverse Strength ◽  
Glass Fiber Reinforced ◽  
Significant Difference ◽  
Post Hoc

Objective.The aim of this study was to evaluate the effect of incorporation of glass fiber, zirconia, and nano-zirconia on the transverse strength of repaired denture base.Materials and Methods.Eighty specimens of heat polymerized acrylic resin were prepared and randomly divided into eight groups (n=10): one intact group (control) and seven repaired groups. One group was repaired with autopolymerized resin while the other six groups were repaired using autopolymerized resin reinforced with 2 wt% or 5 wt% glass fiber, zirconia, or nano-zirconia particles. A three-point bending test was used to measure the transverse strength. The results were analyzed using SPSS and repeated measure ANOVA and post hoc least significance (LSD) test (P≤0.05).Results.Among repaired groups it was found that autopolymerized resin reinforced with 2 or 5 wt% nano-zirconia showed the highest transverse strength (P≤0.05). Repairs with autopolymerized acrylic resin reinforced with 5 wt% zirconia showed the lowest transverse strength value. There was no significant difference between the groups repaired with repair resin without reinforcement, 2 wt% zirconia, and glass fiber reinforced resin.Conclusion.Reinforcing of repair material with nano-zirconia may significantly improve the transverse strength of some fractured denture base polymers.

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