scholarly journals Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Nathaniel J. Kingsbury ◽  
Karen A. McDonald
Keyword(s):  
Recombinant Proteins ◽  
Leaf Tissue ◽  
Downstream Processing ◽  
Vacuum Infiltration ◽  
Production Costs ◽  
High Recovery ◽  
Production Platform ◽  
Homogenate Extraction ◽  
E1 Endoglucanase ◽  
Processing Steps

As a production platform for recombinant proteins, plant leaf tissue has many advantages, but commercialization of this technology has been hindered by high recovery and purification costs. Vacuum infiltration-centrifugation (VI-C) is a technique to obtain extracellularly-targeted products from the apoplast wash fluid (AWF). Because of its selective recovery of secreted proteins without homogenizing the whole tissue, VI-C can potentially reduce downstream production costs. Lab scale experiments were conducted to quantitatively evaluate the VI-C method and compared to homogenization techniques in terms of product purity, concentration, and other desirable characteristics. From agroinfiltratedNicotiana benthamianaleaves, up to 81% of a truncated version of E1 endoglucanase fromAcidothermus cellulolyticuswas recovered with VI-C versus homogenate extraction, and average purity and concentration increases of 4.2-fold and 3.1-fold, respectively, were observed. Formulas were developed to predict recovery yields of secreted protein obtained by performing multiple rounds of VI-C on the same leaf tissue. From this, it was determined that three rounds of VI-C recovered 97% of the total active recombinant protein accessible to the VI-C procedure. The results suggest that AWF recovery is an efficient process that could reduce downstream processing steps and costs for plant-made recombinant proteins.

scholarly journals The use of legume seed for expression and storage of high value proteins

2014 ◽  
Vol 50 (No. 2) ◽  
pp. 69-76
Author(s):  
T. Moravec ◽  
N. Čeřovská
Keyword(s):  
Recombinant Proteins ◽  
Nutritional Value ◽  
Large Scale ◽  
Protein Production ◽  
Production Capacity ◽  
Production Costs ◽  
Foreign Protein ◽  
N Nutrition ◽  
Production Platform ◽  
And Storage

There is an ever growing need for the use of recombinant proteins both in medicine and industry; however their widespread use is limited by the lack of production capacity. Transgenic plants offer the possibility to produce and deliver recombinant proteins on a large scale with low production costs and with minimal purification or enrichment requirements. Among crop plants, legumes have great potential as a protein production platform because of their naturally high protein content, nutritional value, independence of N-nutrition, pollen containment, available processing technology, storage stability etc. There have been several proof-of-principle attempts to produce large amounts of recombinant protein in seed of both soybean and pea, however, our knowledge of processes regulating the foreign protein production and deposition is still limited.

Einbringen von Gewinden in SLM-Bauteile*/Insertion of threads into SLM components – Reliable strategy for inserting threads into additively manufactured components

2019 ◽  
Vol 109 (01-02) ◽  
pp. 24-29
Author(s):  
E. Abele ◽  
T. Scherer ◽  
F. Geßner ◽  
M. Weigold
Keyword(s):  
Additive Manufacturing ◽  
Selective Laser Melting ◽  
Downstream Processing ◽  
Manufacturing Processes ◽  
Laser Melting ◽  
Manufacturing Costs ◽  
Process Reliability ◽  
High Degree ◽  
Design Freedom ◽  
Processing Steps

Additive Fertigungsverfahren zeichnen sich durch große Gestaltungsfreiheit aus, welche die Herstellung komplexer Bauteile ermöglicht. Angesichts hoher Fertigungskosten ist die Prozesssicherheit nachgeordneter Bearbeitungsschritte (wie zum Beispiel die Gewindefertigung) von großer Bedeutung. Der Artikel stellt die Ergebnisse einer Untersuchungsreihe vor, die unterschiedliche Ansätze der Gewindefertigung in Bauteilen aus Stahl behandelt, die mittels Selektivem Laserschmelzverfahren gefertigt wurden.   Additive manufacturing processes are characterized by a high degree of design freedom to enablet the production of complex components. To reduce manufacturing costs, the process reliability of downstream processing steps (e. g. thread production) is of great importance. This article presents the results of a series of investigations dealing with different approaches to thread production in steel components manufactured by selective laser melting

scholarly journals Innovative System for Continuous Microalgae Harvesting by Electrocoagulation/Flocculation and Sedimentation

Proceedings ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 48
Author(s):  
Luiza Mîrţ ◽  
Alexandru Vlaicu ◽  
Alin Vintilă ◽  
Ana-Maria Gălan ◽  
Gabriel Vasilievici ◽  
...  
Keyword(s):  
Downstream Processing ◽  
Production Costs ◽  
Microalgae Harvesting ◽  
Microalgae Culture ◽  
Innovative System

Harvesting is an important part of the downstream processing of the microalgae culture and one of the most price-demanding [1] and represents more than 30% of the overall production costs. [...]

Industrial Application of Through-Process Modelling I: Microstructural Descriptors Required from the Casting Model for Downstream Process Stages

2006 ◽  
Vol 519-521 ◽  
pp. 1783-1788
Author(s):  
Tom Quested ◽  
Mischa Crumbach ◽  
Richard Hamerton
Keyword(s):  
Sensitivity Analysis ◽  
Downstream Processing ◽  
Process Modelling ◽  
Ingot Metallurgy ◽  
Downstream Process ◽  
Model Application ◽  
Processing Steps ◽  
Cast Microstructure ◽  
Theory And Model

This paper contains a review of microstructural through-process modelling (TPM) and the particular role of ingot metallurgy from an industrial perspective, focusing on recent advances in solidification theory, solidification and homogenisation software, and software environments that allow models to interface. To illustrate how as-cast microstructure can impact on downstream processing steps, a sensitivity analysis has been performed on an AA1xxx alloy using an in-house homogenisation model. Thus, knowledge gaps in theory and model application are highlighted.

scholarly journals Potential Use of Eucalyptus Seedling in Recycling of Fish Farming Wastewater in Agriculture

2019 ◽  
pp. 1-11
Author(s):  
Dalva Paulus ◽  
Ezequiel Toffoli ◽  
Celso Eduardo Pereira Ramos ◽  
Claúdia de Andrade Moura ◽  
Dislaine Becker ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Quality Index ◽  
Eucalyptus Grandis ◽  
Leaf Tissue ◽  
Biomass Accumulation ◽  
Fish Farming ◽  
Production Costs ◽  
Nutrient Sources ◽  
Nutrient Contents ◽  
University Of Technology

Aims: The reuse of fish wastewater in agricultural activities such as the production of seedlings in commercial nurseries has great potential to minimize production costs and to reduce environmental impacts due to the inappropriate disposal of this waste. The objective of this study was to evaluate the growth, development and quality of Eucalyptus grandis W. Hill ex Maiden seedlings produced with different wastewater concentrations from fish farming. Study Design:  The fertigation treatments were using fish farming (Tilapia) wastewater from tanks (FW), daily nutrient solution (DNS), and the combinations of 50% FW + 50% DNS, and 25% DNS + 75% FW, applied daily. Place and Duration of Study: The experiment was carried out from August to December 2016 at the Federal University of Technology of Paraná, Brazil. Methodology: The macro and micronutrient contents in leaf tissue, seedling height, stem diameter, the largest root length, leaf area, fresh and dry shoot and root mass and Dickson quality index were all evaluated. Results: The nutrient contents present in the leaf tissue were adequate for the nutritional demand of the eucalyptus. The seedling quality index (DQI) indicated that the daily nutrient solution (0.21) and 50% FW + 50% DNS (0.20) generated balanced seedlings regarding height and biomass accumulation. Conclusion: Fertigation of eucalyptus seedlings can be carried out with wastewater from fish farming, but it is necessary to complement the fertilization with other nutrient sources to produce quality seedlings which are suitable for field transplantation.

scholarly journals Synthetic Metabolic Pathway for the Production of 1-Alkenes from Lignin-derived Molecules

2018 ◽  
Author(s):  
Jin Luo ◽  
Tapio Lehtinen ◽  
Elena Efimova ◽  
Ville Santala ◽  
Suvi Santala
Keyword(s):  
Value Added ◽  
Downstream Processing ◽  
Production Costs ◽  
Acid Decarboxylase ◽  
Acinetobacter Baylyi ◽  
Adaptive Laboratory Evolution ◽  
Tolerant Strain ◽  
In The Wild ◽  
Alkene Synthesis ◽  
Synthetic Metabolic Pathway

AbstractIntegration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce readily collectable product, minimizing production costs. Towards that end, we established the production of semivolatile medium-chain α-olefins from lignin-derived monomers: we constructed 1-undecene synthesis pathway inAcinetobacter baylyiADP1 using ferulate as the sole carbon source. In order to overcome the toxicity of ferulate, we first applied adaptive laboratory evolution, resulting in a highly ferulate-tolerant strain. Next, we demonstrated the 1-undecene production from glucose by heterologously expressing a fatty acid decarboxylase UndA and a thioesterase ‘TesA in the wild type strain. Finally, we constructed the alkene synthesis pathway in the ferulate-tolerant strain. We were able to produce 1-undecene from ferulate and collect the product from the culture headspace without downstream processing. This study demonstrates the potential of bacterial lignin upgradation into value-added products.

The Lomond Field, Block 23/21, UK North Sea

2020 ◽  
Vol 52 (1) ◽  
pp. 511-522 ◽  
Author(s):  
Srmuti Jena ◽  
David Olowoleru
Keyword(s):  
Continental Shelf ◽  
North Sea ◽  
Gas Condensate ◽  
Recovery Factor ◽  
High Recovery ◽  
Integrated Production ◽  
Production Platform ◽  
Production Wells ◽  
Production History ◽  
Very High

AbstractLomond is a gas–condensate field on the east flank of the Central Graben UK Continental Shelf, some 230 km east of Aberdeen in Block 23/21. The field was discovered in 1972 and was developed with nine production wells from an integrated production platform. Lomond is a large salt-induced anticline with four-way dip closure. The reservoir comprises Paleocene turbidite sandstones with the majority of the hydrocarbon volume in the Forties Sandstone Member and the top seal is provided by laterally extensive mudstones of the Sele Formation. The field is structurally compartmentalized with three different hydrocarbon–water contacts, but with the gas leg in pressure communication. Significant reservoir and structural complexities are observed in Lomond Field; however, the production behaviour exhibits classical tank-like depletion behaviour over its production history. With a very high recovery factor to date, the field has produced 883 bcf or 86% of the gas resource initially in place.

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