scholarly journals Synthetic Metabolic Pathway for the Production of 1-Alkenes from Lignin-derived Molecules

2018 ◽  
Author(s):  
Jin Luo ◽  
Tapio Lehtinen ◽  
Elena Efimova ◽  
Ville Santala ◽  
Suvi Santala
Keyword(s):  
Value Added ◽  
Downstream Processing ◽  
Production Costs ◽  
Acid Decarboxylase ◽  
Acinetobacter Baylyi ◽  
Adaptive Laboratory Evolution ◽  
Tolerant Strain ◽  
In The Wild ◽  
Alkene Synthesis ◽  
Synthetic Metabolic Pathway

AbstractIntegration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce readily collectable product, minimizing production costs. Towards that end, we established the production of semivolatile medium-chain α-olefins from lignin-derived monomers: we constructed 1-undecene synthesis pathway inAcinetobacter baylyiADP1 using ferulate as the sole carbon source. In order to overcome the toxicity of ferulate, we first applied adaptive laboratory evolution, resulting in a highly ferulate-tolerant strain. Next, we demonstrated the 1-undecene production from glucose by heterologously expressing a fatty acid decarboxylase UndA and a thioesterase ‘TesA in the wild type strain. Finally, we constructed the alkene synthesis pathway in the ferulate-tolerant strain. We were able to produce 1-undecene from ferulate and collect the product from the culture headspace without downstream processing. This study demonstrates the potential of bacterial lignin upgradation into value-added products.

scholarly journals Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Jong Kwan Lee ◽  
Sujin Kim ◽  
Wonsik Kim ◽  
Sungil Kim ◽  
Seungwoo Cha ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Value Added ◽  
Wild Type ◽  
Adaptive Laboratory Evolution ◽  
Gene Encoding ◽  
Laboratory Evolution ◽  
Tolerant Strain ◽  
Dehydrogenase Gene

Abstract Background Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challenging. In previous attempts to produce lactic acid (LA) from methane, LA production levels were limited in part due to LA toxicity. We solved this problem by generating an LA-tolerant strain, which also contributes to understanding novel LA tolerance mechanisms. Results In this study, we engineered a methanotroph strain Methylomonas sp. DH-1 to produce d-lactic acid (d-LA) from methane. LA toxicity is one of the limiting factors for high-level production of LA. Therefore, we first performed adaptive laboratory evolution of Methylomonas sp. DH-1, generating an LA-tolerant strain JHM80. Genome sequencing of JHM80 revealed the causal gene watR, encoding a LysR-type transcription factor, whose overexpression due to a 2-bp (TT) deletion in the promoter region is partly responsible for the LA tolerance of JHM80. Overexpression of the watR gene in wild-type strain also led to an increase in LA tolerance. When d form-specific lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 was introduced into the genome while deleting the glgA gene encoding glycogen synthase, JHM80 produced about 7.5-fold higher level of d-LA from methane than wild type, suggesting that LA tolerance is a critical limiting factor for LA production in this host. d-LA production was further enhanced by optimization of the medium, resulting in a titer of 1.19 g/L and a yield of 0.245 g/g CH4. Conclusions JHM80, an LA-tolerant strain of Methylomonas sp. DH-1, generated by adaptive laboratory evolution was effective in LA production from methane. Characterization of the mutated genes in JHM80 revealed that overexpression of the watR gene, encoding a LysR-type transcription factor, is responsible for LA tolerance. By introducing a heterologous lactate dehydrogenase gene into the genome of JHM80 strain while deleting the glgA gene, high d-LA production titer and yield were achieved from methane.

scholarly journals Techno-Economic Analysis of a Hyaluronic Acid Production Process Utilizing Streptococcal Fermentation

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 241
Author(s):  
Rafael G. Ferreira ◽  
Adriano R. Azzoni ◽  
Maria Helena Andrade Santana ◽  
Demetri Petrides
Keyword(s):  
Hyaluronic Acid ◽  
Economic Analysis ◽  
Lower Cost ◽  
Value Added ◽  
Production Costs ◽  
Selling Price ◽  
Baseline Scenario ◽  
Adhesive Properties ◽  
Fed Batch Culture ◽  
Economic Analyses

Hyaluronic acid (HA) is a polysaccharide of alternating d-glucuronic acid and N-acetyl-d-glucosamine residues present in the extracellular matrix of connective, epithelial, and nervous tissues. Due to its singular hydrating, rheological and adhesive properties, HA has found numerous cosmetic and medical applications. However, techno-economic analyses of high value-added bioproducts such as HA are scarce in the literature. Here, we present a techno-economic analysis of a process for producing HA using Streptococcus zooepidemicus, simulated in SuperPro Designer. In the baseline scenario, HA is produced by batch fermentation, reaching 2.5 g/L after 24 h. It is then centrifuged, diafiltered, treated with activated carbon and precipitated with isopropanol. The product is suitable for topical formulations and its production cost was estimated as 1115 $/kg. A similar scenario, based on fed-batch culture and assuming a titer of 5.0 g/L, led to a lower cost of 946 $/kg. Moreover, in two additional scenarios, 10% of the precipitated HA is diverted to the production of a highly pure and high-molecular weight HA, suitable for injectable applications. These scenarios resulted in higher capital and operating costs, but also in higher profits, because HA for injectable use has a higher selling price that more than compensates for its higher production costs.

NILAI TAMBAH PENGOLAHAN KANGKUNG HIDROPONIK MENJADI KANGKUNG RENDANG

2020 ◽  
Vol 2 (2) ◽  
pp. 95-106
Author(s):  
Indrawaty Sitepu ◽  
Nurmely Violeta Sitorus
Keyword(s):  
Raw Materials ◽  
Value Added ◽  
Data Retrieval ◽  
Rice Flour ◽  
Production Costs ◽  
Added Value ◽  
Water Spinach ◽  
A Value ◽  
Chicken Eggs

Kangkung hidroponik menjadi kangkung rendang merupakan kegiatan yang dapat meningkatkan nilai tambah, menghasilkan produk yang dapat dikonsumsi, serta menambah pendapatan dan keuntungan produsen.Tujuan penelitian untuk menguraikan apa saja tahapan pengolahan kangkung hidroponik menjadi kangukung rendang, menganalisis biaya produksi, penerimaan, dan pendapatan, menganalisis nilai tambah pengolahan kangkung hidroponik menjadi kangkung rendang, menganalisis apakah usaha pengolahan kangkung hidroponik menjadi kangkung rendang layak diusahakan. Penelitian ini dilakukan di Jalan Bromo lorong Amal Medan Denai Kota Medan. Penentuan daerah penelitian dilakukan secara purposive, Metode pengambilan sampel secara sensus yaitu usaha Syifa Hidroponik dengan pengambilan data ulangan selama 2,5 bualan sebanyak 10 kali ulangan. Hasil penelitian: 1) Tahapan  pengolahan kangkung hidroponik menjadi kangkung rendang yaitu:  Penyediaan bahan baku kangkung hidroponik, kangkung dihaluskan, pengadonan kangkung, telur ayam, tepung beras dan garam, kangkung dikukus, kangkung didinginkan, dipotong-potong, digoreng, pemasakan bumbu rendang, pencampuran kangkung yang digoreng dengan bumbu rendang dan pemasaran. Total biaya pengolahan kangkung hidroponik menjadi kangkung rendang untuk sekali produksi sebesar Rp 545.291,83, penerimaan sebesar Rp 1.500.000,00, per sekali produksi dan pendapatan sebesar Rp 954.708,17 per sekali produksi. Nilai tambah yang dihasilkan dari pengolahan kangkung hidroponik menjadi kangkung rendang tergolong tinggi dengan rasio nilai tambah 75,31% > 50%.Usaha pengolahan kangkung hidroponik menjadi kangkung rendang layak untuk diusahakan dengan nilai R/C rasio 2,75 > 1.  Abstract  Hydroponic water spinach into rendang water spinach is an activity that can increase added value, produce edible products, as well as increase producer income and profits. The purpose of the research is to describe what are the stages of processing hydroponic water spinach into rendang kangukung, analyze production costs, revenue, and income, analyze added value of processing hydroponic water spinach into rendang water spinach, analyzing whether the business of processing hydroponic water spinach into rendang water spinach is worth the effort. This research was conducted in Jalan Bromo Amal Medan Denai alley Medan City. Determination of the study area was done purposively, census sampling method that is Syifa Hydroponic business with retrieval data retrieval for 2.5 boasting as many as 10 replications. The results of the study: 1) The stages of processing hydroponic water spinach into rendang water spinach, namely: Provision of raw materials for hydroponic water spinach, crushed water spinach, stirring water spinach, chicken eggs, rice flour and salt, steamed water spinach, water spinach water spinach, cut into pieces, fried, fried spicy water spinach, cooking water spinach kale, chicken egg, rice flour and salt, steamed water spinach, water spinach kangkung cooled, cut, fried, cooking spices, rendang, mixing fried kale with spicy rendang and marketing. The total cost of processing hydroponic water spinach into rendang water spinach for one production is Rp. 545,291.83, revenue is Rp. 1,500,000.00, per production and income is Rp. 954,708.17 per production. The added value generated from the processing of hydroponic water spinach into rendang water spinach is classified as high with a value added ratio of 75.31%> 50%. The business of processing hydroponic water spinach into rendang water spinach is feasible to be cultivated with an R / C ratio of 2.75> 1.  

Fresh-cut apples: Challenges and opportunities for multi-disciplinary research

2006 ◽  
Vol 86 (Special Issue) ◽  
pp. 1361-1368 ◽  
Author(s):  
Peter M. A. Toivonen
Keyword(s):  
Value Chain ◽  
Fungal Pathogens ◽  
Value Added ◽  
Research Strategy ◽  
Production Costs ◽  
Food Microbiology ◽  
Research Activities ◽  
Industry Practice ◽  
Emerging Issues ◽  
Fresh Cut

The fresh-cut apple industry has recently become established in North America. The future looks very bright for this value-added fruit product, however its growth requires improvements in quality and reduction of production costs. The existing industry has grown from concept to reality in response to research activities in anti-browning dips, package technology, sensory analysis, postharvest physiology, postharvest pathology and food microbiology. Several examples of how these critical research inputs have impacted and continue to impact on the industry practice are discussed. The next generation of questions and challenges posed by the industry will require some new inputs. Research in several disciplines will be need accessed to resolve the emerging issues. Traditional breeding effort and/or molecular technologies will be needed to provide non-browning fruit, fruit with better processing characteristics such as small cores to reduce waste and improved flavour and nutrition retention after cutting. With new cultivars, further work will be required to understand the commercial handling of each, including selection of harvest maturity and optimization of storage protocols. Also, pre-harvest factors such as phosphorus nutrition will need to be investigated further in terms of their effects on fruit tissue and membrane stability. Management of fungal pathogens is a key issue that needs to be studied from the field through storage and after cutting and packaging of the sliced fruit. The very nature of fresh-cut fruit has and continues to require a coordinated multi-disciplinary research strategy. Key words: Value-chain, value-added, apples, fresh-cut, integrated research

scholarly journals Impact of Distance between Strip Roads on Productivity and Costs of a Forwarder in Commercial Thinning of Pinus Taeda Stands

2020 ◽  
Vol 41 (2) ◽  
pp. 243-249
Author(s):  
Oscar Manuel de Jesús Vera Cabral ◽  
Eduardo da Silva Lopes ◽  
Carla Krulikowski Rodrigues ◽  
Afonso Figueiredo Filho
Keyword(s):  
Pinus Taeda ◽  
Value Added ◽  
Wood Products ◽  
Production Costs ◽  
Working Cycle ◽  
Time Motion ◽  
Commercial Thinning ◽  
Selective Harvest ◽  
Thinning Operations ◽  
The Impact

Demand for higher value-added wood products stimulates research for new, mainly mechanized, thinning operations in order to increase productivity and reduce production costs. In this context, the aim of this study was to evaluate the impact of distance between strip roads on forwarder productivity and costs of thinning operations in Pinus taeda stands. The study was carried out in 10-year-old Pinus taeda stands located in Parana State, Brazil. Two thinning methods were evaluated: (1) TH5: systematic harvest in every fifth tree row and selective harvest in adjacent rows; and (2) TH7: systematic harvest in every seventh tree row and selective harvest in adjacent rows. Working cycle times, productivity and costs were determined through a time-motion study of the forwarder. The additional variables evaluated were wood assortments (industrial wood and energy wood) and extraction distances (50, 100, 150 and 200 m), and mean values were compared between thinning methods using t tests for independent samples (α=0.05). Loading and unloading elements consumed the most time in the working cycle, with lower participation time in TH7 due to greater availability of logs along the strip roads (higher pile volumes), influencing total cycle time up to the mean distance of 150 m for both assortments. TH7 consequently showed 6% higher productivity, its energy yield was 5.3% lower and its production cost was 3.0% lower.

scholarly journals Demonstration and Assessment of Purification Cascades for the Separation and Valorization of Hemicellulose from Organosolv Beechwood Hydrolyzates

Membranes ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 82
Author(s):  
Roy Nitzsche ◽  
Hendrik Etzold ◽  
Marlen Verges ◽  
Arne Gröngröft ◽  
Matthias Kraume
Keyword(s):  
Energy Efficiency ◽  
Hydrothermal Treatment ◽  
Experimental Work ◽  
Value Added ◽  
Aspen Plus ◽  
High Potential ◽  
Production Costs ◽  
Specific Production ◽  
The Cost ◽  
Value Added Products

Hemicellulose and its derivatives have a high potential to replace fossil-based materials in various high-value-added products. Within this study, two purification cascades for the separation and valorization of hemicellulose and its derived monomeric sugars from organosolv beechwood hydrolyzates (BWHs) were experimentally demonstrated and assessed. Purification cascade 1 included hydrothermal treatment for converting remaining hemicellulose oligomers to xylose and the purification of the xylose by nanofiltration. Purification cascade 2 included the removal of lignin by adsorption, followed by ultrafiltration for the separation and concentration of hemicellulose. Based on the findings of the experimental work, both cascades were simulated on an industrial scale using Aspen Plus®. In purification cascade 1, 63% of the oligomeric hemicellulose was hydrothermally converted to xylose and purified by nanofiltration to 7.8 t/h of a xylose solution with a concentration of 200 g/L. In purification cascade 2, 80% of the lignin was removed by adsorption, and 7.6 t/h of a purified hemicellulose solution with a concentration of 200 g/L was obtained using ultrafiltration. The energy efficiency of the cascades was 59% and 26%, respectively. Furthermore, the estimation of specific production costs showed that xylose can be recovered from BWH at the cost of 73.7 EUR/t and hemicellulose at 135.1 EUR/t.

scholarly journals Application of Microalgal Stress Responses in Industrial Microalgal Production Systems

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 30
Author(s):  
Jia Wang ◽  
Yuxin Wang ◽  
Yijian Wu ◽  
Yuwei Fan ◽  
Changliang Zhu ◽  
...  
Keyword(s):  
Stress Responses ◽  
Production Systems ◽  
Strain Improvement ◽  
Value Added ◽  
Product Yield ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Theoretical Support ◽  
Further Development ◽  
Value Added Products

Adaptive laboratory evolution (ALE) has been widely utilized as a tool for developing new biological and phenotypic functions to explore strain improvement for microalgal production. Specifically, ALE has been utilized to evolve strains to better adapt to defined conditions. It has become a new solution to improve the performance of strains in microalgae biotechnology. This review mainly summarizes the key results from recent microalgal ALE studies in industrial production. ALE designed for improving cell growth rate, product yield, environmental tolerance and wastewater treatment is discussed to exploit microalgae in various applications. Further development of ALE is proposed, to provide theoretical support for producing the high value-added products from microalgal production.

scholarly journals Innovative System for Continuous Microalgae Harvesting by Electrocoagulation/Flocculation and Sedimentation

Proceedings ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 48
Author(s):  
Luiza Mîrţ ◽  
Alexandru Vlaicu ◽  
Alin Vintilă ◽  
Ana-Maria Gălan ◽  
Gabriel Vasilievici ◽  
...  
Keyword(s):  
Downstream Processing ◽  
Production Costs ◽  
Microalgae Harvesting ◽  
Microalgae Culture ◽  
Innovative System

Harvesting is an important part of the downstream processing of the microalgae culture and one of the most price-demanding [1] and represents more than 30% of the overall production costs. [...]

Applying the concept of entrepreneurial ecosystems in Estonia

2019 ◽  
Vol 63 (2-4) ◽  
pp. 134-145
Author(s):  
Merli Reidolf ◽  
Merle Küttim ◽  
Aleksandr Michelson ◽  
Helena Rozeik ◽  
Marianne Kallaste
Keyword(s):  
Knowledge Base ◽  
Qualitative Methodology ◽  
Economic Value ◽  
Value Added ◽  
Production Costs ◽  
Natural Ecosystems ◽  
Theoretical Understanding ◽  
Entrepreneurial Ecosystems ◽  
Sectoral Differences ◽  
The Difference

Abstract Similar to natural ecosystems, entrepreneurs and other entrepreneurial actors are locally interdependent and affect the performance of each other. Mostly economically larger and more successful regions have been studied by using the framework of entrepreneurial ecosystems (EEs). The current study aims at further developing the theoretical understanding of EEs and applying the proposed EE model in the context of Estonia. In the study, a conceptual model of an EE is proposed, the uniqueness of which lies in merging three aspects into a single framework: the nine components of an EE, its value-added, and its phase of development. The model was applied for analysing by means of a qualitative methodology four sector-based subEEs in Estonia. The study showed that even in a small country like Estonia the components of the EE were partly similar, but also partly unique for the subEEs. This was not due to the location, but because of sectoral differences. The difference of the development phase seemed to be related to the knowledge base of the sector. The policy relevance of the model is that it allows looking at the elements of an EE as well as its economic value-added and development prospects. The EE approach allows addressing jointly the difficulties and challenges in the development of EEs, including the issues of digitalisation, skilled labour, and production costs, which would allow the subEEs to develop into mature and resilient networks. Not all the components of an EE have to be equally strong, but their interconnectedness determines the strength of an EE. Policy solutions need to be tailored to the needs of specific subEEs based on the knowledge base and inner dynamics of the sectors.

scholarly journals A Case of Adaptive Laboratory Evolution (ALE): Biodegradation of Furfural by Pseudomonas pseudoalcaligenes CECT 5344

Genes ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 499 ◽  
Author(s):  
M. Isabel Igeño ◽  
Daniel Macias ◽  
Rafael Blasco
Keyword(s):  
Carbon Sources ◽  
Value Added ◽  
Cost Effective ◽  
Building Blocks ◽  
Yeast Fermentation ◽  
Adaptive Laboratory Evolution ◽  
Pseudomonas Pseudoalcaligenes ◽  
Hydroxymethyl Furfural ◽  
Double Recombination ◽  
Lag Period

Pseudomonas pseudoalcaligenes CECT 5344 is a bacterium able to assimilate cyanide as a nitrogen source at alkaline pH. Genome sequencing of this strain allowed the detection of genes related to the utilization of furfurals as a carbon and energy source. Furfural and 5-(hydroxymethyl) furfural (HMF) are byproducts of sugars production during the hydrolysis of lignocellulosic biomass. Since they inhibit the yeast fermentation to obtain bioethanol from sugars, the biodegradation of these compounds has attracted certain scientific interest. P. pseudoalcaligenes was able to use furfuryl alcohol, furfural and furoic acid as carbon sources, but after a lag period of several days. Once adapted, the evolved strain (R1D) did not show any more prolonged lag phases. The transcriptomic analysis (RNA-seq) of R1D revealed a non-conservative punctual mutation (L261R) in BN5_2307, a member of the AraC family of activators, modifying the charge of the HTH region of the protein. The inactivation of the mutated gene in the evolved strain by double recombination reverted to the original phenotype. Although the bacterium did not assimilate HMF, it transformed it into value-added building blocks for the chemical industry. These results could be used to improve the production of cost-effective second-generation biofuels from agricultural wastes.

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