scholarly journals Reliability of a Fully Automated Interpretation ofγ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Juliane Heydenreich ◽  
Christoph Otto ◽  
Frank Mayer ◽  
Anja Carlsohn
Keyword(s):  
Dna Damage ◽  
Healthy Subjects ◽  
Background Level ◽  
Limits Of Agreement ◽  
Trained Subjects ◽  
Coefficients Of Variation ◽  
Automated Interpretation ◽  
Baseline Levels ◽  
Exercise Induced ◽  
Healthy Males

Background. Analysis ofγ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage. However, baseline levels and day-to-day variability ofγ-H2AX foci have not been investigated in healthy subjects at rest.Methods. Blood was taken from eight moderately trained healthy males (29 ± 3 yrs, 1.84 ± 0.03 m, and 85 ± 6 kg) at two separate days (M1/M2) after 24-hour exercise cessation. Number ofγ-H2AX foci per 100 lymphocytes (N), number of foci per affected lymphocyte (NAL), percentage of affected lymphocytes (PAL), and diameter (D) ofγ-H2AX foci were analyzed (mean ± SD). Differences between M1 and M2 were analyzed using pairedt-tests (α= 0.05). Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA).Results. There were no statistically significant differences between M1 (N: 7.6 ± 4.4, NAL: 1.2 ± 0.2, PAL: 5.9 ± 2.6%, and D: 0.63 ± 0.07) and M2 (N: 8.4 ± 4.6, NAL: 1.3 ± 0.1, PAL: 6.9 ± 4.2%, and D: 0.66 ± 0.06). CV was calculated to be 98.5% (N), 88.9% (PAL), 11.3% (NAL), and 8.0% (D). Bias (LoA) was 0.75 (−15.2/13.7), −0.02 (−0.36/0.33), −1.0 (−11.9/9.9), and −0.04 (−0.16/0.09), respectively.Conclusions. Background level in healthy subjects is approximately 0.07 to 0.09γ-H2AX foci/cell. NAL and D are reliable measures.

scholarly journals Exercise-Induced Release of Cardiac Troponins in Adolescent vs. Adult Swimmers

2021 ◽  
Vol 18 (3) ◽  
pp. 1285
Author(s):  
Rafel Cirer-Sastre ◽  
Francisco Corbi ◽  
Isaac López-Laval ◽  
Luis Enrique Carranza-García ◽  
Joaquín Reverter-Masià
Keyword(s):  
Cardiac Troponin ◽  
Healthy Subjects ◽  
Troponin T ◽  
Training Experience ◽  
Post Exercise ◽  
Baseline Values ◽  
Exercise Induced ◽  
Cardiac Troponins ◽  
Similar Peak ◽  
Apparently Healthy

To examine the exercise-induced release of cardiac troponin T (cTnT) in adolescent and adult swimmers. Thirty-two trained male (18 adolescents, 14 adults) swam at maximal pace in a 45 min distance trial, and blood samples were drawn before, immediately and 3 h after exercise for subsequent cTnT analysis and comparison. Having comparable training experience and baseline values of cTnT (p = 0.78 and p = 0.13), adults exercised at lower absolute and relative intensity (p < 0.001 and p < 0.001, respectively), but presented higher immediate cTnT after exercise than adolescents (p < 0.001). Despite that, peak concentrations were observed at 3 h post exercise and peak elevations were comparable between groups (p = 0.074). Fourteen (44%) apparently healthy subjects exceeded the cutoff value for myocardial infarction (MI). Adolescents presented a delayed elevation of cTnT compared with adults, but achieved similar peak values.

Circadian influence on effect of propranolol on exercise-induced tachycardia in healthy subjects

1990 ◽  
Vol 38 (2) ◽  
Author(s):  
A. Fujimura ◽  
Y. Kumagai ◽  
K. Sugimoto ◽  
H. Nakashima ◽  
H. Kajiyama ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Exercise Induced

Acute effects of blood flow restriction on exercise-induced free radical production in young and healthy subjects

2018 ◽  
Vol 52 (4) ◽  
pp. 446-454 ◽  
Author(s):  
Christoph Centner ◽  
Denise Zdzieblik ◽  
Patrick Dressler ◽  
Bruno Fink ◽  
Albert Gollhofer ◽  
...  
Keyword(s):  
Blood Flow ◽  
Free Radical ◽  
Healthy Subjects ◽  
Blood Flow Restriction ◽  
Acute Effects ◽  
Free Radical Production ◽  
Flow Restriction ◽  
Radical Production ◽  
Exercise Induced

scholarly journals Exercise induced changes in T1, T2 relaxation times and blood flow in the lower extremities in healthy subjects

2013 ◽  
Vol 15 (S1) ◽  
Author(s):  
Juliet Varghese ◽  
Debbie Scandling ◽  
Chikako Ono ◽  
Ashish Aneja ◽  
William A Kay ◽  
...  
Keyword(s):  
Blood Flow ◽  
Healthy Subjects ◽  
Relaxation Times ◽  
Lower Extremities ◽  
T2 Relaxation ◽  
Exercise Induced ◽  
Induced Changes

Modulation of Exercise-Induced Hypoalgesia Following an Exercise Intervention in Healthy Subjects

Pain Medicine ◽  
2020 ◽  
Vol 21 (12) ◽  
pp. 3556-3566
Author(s):  
Simon Hansen ◽  
Rasmus Christiansen Dalgaard ◽  
Pernille Schlosser Mikkelsen ◽  
Mathias Brandhøj Sørensen ◽  
Kristian Kjær Petersen
Keyword(s):  
Physical Performance ◽  
Healthy Subjects ◽  
Exercise Intervention ◽  
Military Training ◽  
Pressure Pain Threshold ◽  
Pain Mechanisms ◽  
Performance Capacity ◽  
Physical Performance Capacity ◽  
Exercise Induced ◽  
Chronic Pain Conditions

Abstract Background Exercise is recommended to promote and maintain health and as treatment for more than 25 diseases and pain conditions. Exercise-induced hypoalgesia (EIH), a measure of descending pain inhibitory control, has been found to be impaired in some chronic pain conditions, but it is currently unclear if EIH is modifiable. This study investigated whether a long-term exercise intervention could modulate EIH in healthy subjects. Methods In 38 healthy subjects, EIH was assessed as change in pressure pain threshold (PPT) after a three-minute isometric wall squat within the first week and after approximately seven weeks of military training (MT). Further, temporal summation of pain (TSP) and Knee injury and Osteoarthritis Outcome Score (KOOS) were assessed. Physical performance capacity was assessed using the Endurance 20-m shuttle run fitness test (20MSR). Hypoalgesic (EIH &gt; 0.0 kPa) and hyperalgesic (EIH ≤ 0.0 kPa) subgroups were defined based on baseline EIH. Change in EIH following MT was used as the primary outcome. Results Increased EIH (P = 0.008), PPT (P &lt; 0.003), and 20MSR (P &lt; 0.001) were found following MT, with no changes in TSP and KOOS (P &gt; 0.05). Subjects with a hyperalgesic EIH response at baseline (26% of the participants) presented significantly improved EIH following MT (P = 0.010). Finally, an association between 20MRS change and EIH change was found (r = 0.369, P = 0.023). Conclusions MT increased EIH, especially in subjects who demonstrated a hyperalgesic response at baseline. Improvement in physical performance capacity was associated with an improvement in EIH, indicating that improvement in physical performance capacity may improve central pain mechanisms.

Glucagon radioimmunoassay with use of antiserum to glucagon C-terminal fragment.

1981 ◽  
Vol 27 (10) ◽  
pp. 1690-1697 ◽  
Author(s):  
T Nishino ◽  
T Kodaira ◽  
S Shin ◽  
K Imagawa ◽  
K Shima ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Assay System ◽  
Glucose Load ◽  
Arginine Infusion ◽  
Pancreatic Glucagon ◽  
Terminal Fragment ◽  
Specific Radioimmunoassay ◽  
Dog Plasma ◽  
Coefficients Of Variation

Abstract In this pancreatic-glucagon-specific radioimmunoassay we used C-terminal-region-specific antiserum. OAL-123, produced against a 19-29 C-terminal fragment of porcine glucagon. On measurement of pooled plasma the ranges for intra- and inter-assay coefficients of variation were 4.8-8.1% and 7.5-10.7%, respectively. The concentration of immunoreactive glucagon in plasma of healthy subjects, as measured with the OAL-123 assay system, was 87.9 (SD 23.8) ng/L. Measurement of the same plasma samples with the 30K assay system (30K being an antiserum highly specific for pancreatic glucagon) showed a comparable value, 86.2 (SD 26.3) ng/L. We followed changes in human and dog plasma immunoreactive glucagon concentrations on arginine infusion and after glucose load, using the OAL-123 and the 30K assay systems, with identical results. Combining other results of comparative immunochemical characterization of the OAL-123 and 30K assay systems, we confirmed that the antisera raised against the C-terminal fragment of glucagon can be used in radioimmunoassay of pancreatic glucagon.

scholarly journals Parathormone Levels in a Middle-Eastern Healthy Population Using 2nd and 3rd Generation PTH Assays

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Marie-Hélène Gannagé-Yared ◽  
Marie-Noëlle Kallas-Chémaly ◽  
Ghassan Sleilaty
Keyword(s):  
Vitamin D ◽  
Healthy Subjects ◽  
Reference Range ◽  
Middle Eastern ◽  
Healthy Population ◽  
Strongly Correlated ◽  
Limits Of Agreement ◽  
25 Oh Vitamin D ◽  
D Values ◽  
Normal Calcium

Background. The purpose of the current study is to determine PTH reference values in vitamin-D-replete Lebanese adults using 2nd and 3rd generation PTH assays and to look at the factors that affect PTH variations. Methods. Fasting PTH was measured using 2nd and 3rd generation Diasorin PTH assays in 339 vitamin-D-replete healthy subjects aged 18 to 63 years (230 men and 109 women) who have normal calcium levels and an eGFR ≥60 ml/mn. 25-OH vitamin D (25(OH)D) was measured using the Diasorin assay. Results. For the 2nd PTH generation, median (IQR) levels were 48.9 (34.9–66.0) pg/ml, and its 2.5th–97.5th percentile values were 19.7–110.5 pg/ml for 25(OH)D values between 20 and 30 ng/ml, and 19.7–110.7 pg/ml for 25(OH)D values ≥30 ng/ml. For the 3rd PTH generation, the median (IQR) values were 23.9 (17.7–30.5) pg/ml, and its 2.5th–97.5th percentile values were, respectively, 9.2 and 50.2 pg/ml for 25(OH)D values between 20 and 30 ng/ml, and 8.4 and 45.4 pg/ml for 25(OH)D values ≥30 ng/ml. The median (IQR) serum 25(OH)D levels were 27.5 (23.8–32.7) ng/ml. 2nd and 3rd generation PTH values are strongly correlated (r = 0.96, p<0.0001), but poorly concordant (Lin’s concordance coefficient 0.365, 95% CI: 0.328–0.401) with observations beyond the 95% Bland–Altman limits of agreement. 2nd and 3rd generation PTH levels did not differ according to gender and were significantly correlated with age but not with 25(OH)D and serum calcium levels. Conclusion. Lebanese adult healthy subjects have higher 2nd and 3rd generation PTH levels compared with the reference range provided by the manufacturer. The reference range was not influenced by changing the 25(OH)D cutoff. The clinical significance of the higher PTH levels in our population should be investigated.

scholarly journals SOD Derived from Bacillus amyloliquefaciens GF423 Has Protection Against Exercise-Induced Oxidative Stress in Healthy Subjects

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 764-764
Author(s):  
Yea-eun Nam ◽  
Yunsoo Kim ◽  
Yeni Lim ◽  
Hye Jin Kim ◽  
Oran Kwon
Keyword(s):  
Oxidative Stress ◽  
Healthy Subjects ◽  
Antioxidant Defense ◽  
Maximal Oxygen Consumption ◽  
Intervention Group ◽  
Lipid Peroxides ◽  
Experimental Period ◽  
Cellular Damage ◽  
Double Blind ◽  
Exercise Induced

Abstract Objectives Excessive reactive oxygen species (ROS) can cause cellular damage, causing a variety of degenerative diseases such as atherosclerosis, ischemic heart disease, and cancer. SOD is thought to play a central role in scavenging ROS generated in cells by enhancing the antioxidant defense system, including catalase and glutathione peroxidase. This study aims to test the hypothesis that exogenous SOD administration can help to protect against oxidative stress encountered at very early stages in the daily life of healthy subjects. Methods A total of 80 healthy adults were assigned to either an intervention group consuming B. amyloliquefaciens GF423 SOD (250 IU/capsule) daily for 8 weeks or a placebo in a randomized, double-blind and parallel design. Aerobic exercise by a treadmill for 30 minutes at an intensity of 60% of the maximal oxygen consumption (VO2max) of each subject was used to induce oxidative stress at the beginning and end of the experimental period. Blood and urine samples were collected immediately after and 30 min after the exercise challenge to measure biochemical markers related to oxidative stress and inflammation. Results A single administration of exogenous SOD induced a marked decrease in urinary lipid peroxides and plasma pro-inflammatory cytokines as compared to placebo administration. Furthermore, repeated administration of exogenous SOD for eight weeks resulted in a significant improvement of erythrocyte redox balance. Conclusions These findings suggest that the supply of exogenous SOD may be useful to enhance the antioxidant defense capacity and anti-inflammatory response in response to exercise-induced oxidative stress. Funding Sources This work was supported by the Bio-Synergy Research Project (NRF-2012M3A9C4048761) from the Ministry of Science, ICT, and Future Planning, and the BK21PLUS (Brain Korea 21 plus) program (22a20130012143) from the Ministry of Education and the GenoFocus Inc, Republic of Korea.

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