scholarly journals The Study of Naphthoquinones and Their Complexes with DNA by Using Raman Spectroscopy and Surface Enhanced Raman Spectroscopy: New Insight into Interactions of DNA with Plant Secondary Metabolites

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Veronika Vaverkova ◽  
Oldrich Vrana ◽  
Vojtech Adam ◽  
Tomas Pekarek ◽  
Josef Jampilek ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Secondary Metabolites ◽  
Plant Secondary Metabolites ◽  
Analytical Techniques ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cellular Level ◽  
Cytotoxic Action ◽  
Structure Sensitive ◽  
Surface Enhanced Raman ◽  
Cytotoxic Compounds

Naphthoquinones represent the group of plant secondary metabolites with cytotoxic properties based on their ability to generate reactive oxygen species and interfere with the processes of cell respiration. Due to this fact, the possible cytotoxic mechanisms on cellular and subcellular levels are investigated intensively. There are many targets of cytotoxic action on the cellular level; however, DNA is a critical target of many cytotoxic compounds. Due to the cytotoxic properties of naphthoquinones, it is necessary to study the processes of naphthoquinones, DNA interactions (1,4-naphthoquinone, binapthoquinone, juglone, lawsone, plumbagin), especially by using modern analytical techniques. In our work, the Raman spectroscopy was used to determine the possible binding sites of the naphthoquinones on the DNA and to characterize the bond of naphthoquinone to DNA. Experimental data reveals the relationships between the perturbations of structure-sensitive Raman bands and the types of the naphthoquinones involved. The modification of DNA by the studied naphthoquinones leads to the nonspecific interaction, which causes the transition of B-DNA into A-DNA conformation. The change of the B-conformation of DNA for all measured DNA modified by naphthoquinones except plumbagin is obvious.

Raman Spectroscopy for the Study of XVI-XVII Centuries Colonial Paintings

2014 ◽  
Vol 1618 ◽  
pp. 141-151 ◽  
Author(s):  
Ma. A. García-Bucio ◽  
E. Casanova-González ◽  
J. L. Ruvalcaba-Sil
Keyword(s):  
Raman Spectroscopy ◽  
San Francisco ◽  
Reference Materials ◽  
Analytical Techniques ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Signal ◽  
Invasive Approach ◽  
Full Characterization ◽  
Surface Enhanced Raman ◽  
Historical Objects

ABSTRACTOutstanding information about the material composition and pictorial techniques of the New Spain Colonial painting can be obtained via a full characterization using a set of analytical techniques. Given the cultural importance of this painting, a non-invasive approach is preferred. Moreover, the preparation and use of reference materials using original recipes is necessary for a correct interpretation of the spectroscopic data from historical objects. Here, we present the results obtained via an in-situ Raman spectroscopic analysis of a set of pictorial reference materials, created according to XVI and XVII centuries’ recipes. Several difficulties were encountered, such as the low Raman detection signal, an intrinsic fluorescence of the material, and in some cases even laser-induced degradation. For this reason, the usual molecular Raman analysis was extended to Surface Enhanced Raman Spectroscopy (SERS), which enhances the Raman signal and quenches the fluorescence. It was then applied to the analysis of two wood paintings from the ex-convent San Francisco Tepeyanco, in Tlaxcala.

scholarly journals The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Pin Gao ◽  
Bing Han ◽  
Ye Du ◽  
Gang Zhao ◽  
Zhigang Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Cancer Diagnosis ◽  
Breast Cancer Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cellular Level ◽  
Clinical Tool ◽  
Normal Tissues ◽  
Surface Enhanced Raman

Raman spectroscopy has been widely used as an important clinical tool for real-time in vivo cancer diagnosis. Raman information can be obtained from whole organisms and tissues, at the cellular level and at the biomolecular level. The aim of this paper is to review the newest developments of Raman spectroscopy in the field of breast cancer diagnosis and treatment. Raman spectroscopy can distinguish malignant tissues from noncancerous/normal tissues and can assess tumor margins or sentinel lymph nodes during an operation. At the cellular level, Raman spectra can be used to monitor the intracellular processes occurring in blood circulation. At the biomolecular level, surface-enhanced Raman spectroscopy techniques may help detect the biomarker on the tumor surface as well as evaluate the efficacy of anticancer drugs. Furthermore, Raman images reveal an inhomogeneous distribution of different compounds, especially proteins, lipids, microcalcifications, and their metabolic products, in cancerous breast tissues. Information about these compounds may further our understanding of the mechanisms of breast cancer.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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