scholarly journals Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata)

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Karin Risse ◽  
Karen Schlez ◽  
Tobias Eisenberg ◽  
Christina Geiger ◽  
Anna Balbutskaya ◽  
...  
Keyword(s):  
16S Rdna ◽  
Reference Strain ◽  
Species Identity ◽  
Giraffa Camelopardalis ◽  
Maldi Tof Ms ◽  
Encoding Gene ◽  
Sequence Similarities ◽  
Tof Ms ◽  
Phenotypic And Genotypic Characterization

The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483T of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

scholarly journals Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli

2013 ◽  
Vol 65 (2) ◽  
pp. 585-593 ◽  
Author(s):  
Tatjana Popovic ◽  
Dragana Josic ◽  
Mira Starovic ◽  
P. Milovanovic ◽  
N. Dolovac ◽  
...  
Keyword(s):  
16S Rdna ◽  
Xanthomonas Campestris ◽  
Reference Strain ◽  
Tween 80 ◽  
Genotypic Characterization ◽  
Hypodermic Syringe ◽  
Xanthomonas Campestris Pv Campestris ◽  
Reference Strains ◽  
Phenotypic And Genotypic Characterization

Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35?C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.

Characterization of polyethers using different methods: SFC, LAC and SEC with different detectors, and MALDI-TOF-MS

1996 ◽  
Vol 110 (1) ◽  
pp. 231-240 ◽  
Author(s):  
Bernd Trathnigg ◽  
Bernhard Maier ◽  
Günther Schulz ◽  
Ralph-Peter Krüger ◽  
Ulrich Just
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Multidimensional Characterization of α,ω-Telechelic Poly(ε-caprolactone)s via Online Coupling of 2D Chromatographic Methods (LC/SEC) and ESI-TOF/MALDI-TOF-MS

2012 ◽  
Vol 45 (24) ◽  
pp. 9779-9790 ◽  
Author(s):  
Haitham Barqawi ◽  
Elena Ostas ◽  
Bo Liu ◽  
Jean-François Carpentier ◽  
Wolfgang H. Binder
Keyword(s):  
Maldi Tof Ms ◽  
Chromatographic Methods ◽  
Maldi Tof ◽  
Tof Ms

Synthesis and characterization of polycarbodiimides by MALDI-TOF MS and NMR spectroscopy: kinetic and mechanism study

2017 ◽  
Vol 75 (6) ◽  
pp. 2657-2670
Author(s):  
Albert Morell Garcia ◽  
Francisco Medina Cabello ◽  
Antonio Torrelles Nogués
Keyword(s):  
Nmr Spectroscopy ◽  
Synthesis And Characterization ◽  
Mechanism Study ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Use of electrophoretic techniques and MALDI–TOF MS for rapid and reliable characterization of bacteria: analysis of intact cells, cell lysates, and “washed pellets”

2013 ◽  
Vol 405 (10) ◽  
pp. 3165-3175 ◽  
Author(s):  
Jiří Šalplachta ◽  
Anna Kubesová ◽  
Dana Moravcová ◽  
Marie Vykydalová ◽  
Sándor Süle ◽  
...  
Keyword(s):  
Intact Cells ◽  
Maldi Tof Ms ◽  
Cell Lysates ◽  
Maldi Tof ◽  
Electrophoretic Techniques ◽  
Tof Ms

scholarly journals Molecular Weight Characterization of Humic Substances by MALDI-TOF-MS

2004 ◽  
Vol 52 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Toshiyuki SHINOZUKA ◽  
Mitsuhiro SHIBATA ◽  
Tatsuaki YAMAGUCHI
Keyword(s):  
Molecular Weight ◽  
Humic Substances ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Characterization of immunoglobulins through analysis of N-glycopeptides by MALDI-TOF MS

Methods ◽  
2016 ◽  
Vol 104 ◽  
pp. 170-181 ◽  
Author(s):  
Emy Komatsu ◽  
Marjorie Buist ◽  
Rini Roy ◽  
Andrey Giovanni Gomes de Oliveira ◽  
Edward Bodnar ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

MALDI-TOF-MS Copolymer Analysis:  Characterization of a Poly(dimethylsiloxane)-co-Poly(hydromethylsiloxane) as a Precursor of a Functionalized Silicone Graft Copolymer

1998 ◽  
Vol 31 (8) ◽  
pp. 2468-2474 ◽  
Author(s):  
S. Servaty ◽  
W. Köhler ◽  
W. H. Meyer ◽  
C. Rosenauer ◽  
J. Spickermann ◽  
...  
Keyword(s):  
Graft Copolymer ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms ◽  
Copolymer Analysis

Characterization of IgG N-glycome profile in colorectal cancer progression by MALDI-TOF-MS

2018 ◽  
Vol 181 ◽  
pp. 225-237 ◽  
Author(s):  
Si Liu ◽  
Liming Cheng ◽  
Yang Fu ◽  
Bi-Feng Liu ◽  
Xin Liu
Keyword(s):  
Colorectal Cancer ◽  
Cancer Progression ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Colorectal Cancer Progression ◽  
Tof Ms
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