scholarly journals Supplementation with Sodium Selenite and Selenium-Enriched Microalgae Biomass Show Varying Effects on Blood Enzymes Activities, Antioxidant Response, and Accumulation in Common Barbel (Barbus barbus)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Antonín Kouba ◽  
Josef Velíšek ◽  
Alžběta Stará ◽  
Jiří Masojídek ◽  
Pavel Kozák
Keyword(s):  
Blood Plasma ◽  
Sodium Selenite ◽  
Control Diet ◽  
Antioxidant Response ◽  
Fish Growth ◽  
Biologically Active ◽  
Fish Mortality ◽  
Barbus Barbus ◽  
Microalgae Biomass ◽  
In Blood Plasma

Yearling common barbel (Barbus barbusL.) were fed four purified casein-based diets for 6 weeks in outdoor cages. Besides control diet, these were supplemented with 0.3 mg kg−1dw selenium (Se) from sodium selenite, or 0.3 and 1.0 mg kg−1from Se-enriched microalgae biomass (Chlorella), a previously untested Se source for fish. Fish mortality, growth, Se accumulation in muscle and liver, and activity of selected enzymes in blood plasma, muscle, liver, and intestine were evaluated. There was no mortality, and no differences in fish growth, among groups. Se concentrations in muscle and liver, activity of alanine aminotransferase and creatine kinase in blood plasma, glutathione reductase (GR) in muscle, and GR and catalase in muscle and liver suggested that selenium from Se-enrichedChlorellais more readily accumulated and biologically active while being less toxic than sodium selenite.

scholarly journals Effect of high levels of organic selenium on glutation-peroxidase (GSH-Px) activity in blood plasma of broilers

2005 ◽  
Vol 59 (3-4) ◽  
pp. 383-390 ◽  
Author(s):  
Mirjana Joksimovic-Todorovic ◽  
Zivan Jokic ◽  
Zlatan Sinovec
Keyword(s):  
Blood Plasma ◽  
Sodium Selenite ◽  
Control Group ◽  
Organic Selenium ◽  
Fattening Period ◽  
The Individual ◽  
Selenized Yeast ◽  
Broiler Feed ◽  
Plasma Activity ◽  
In Blood Plasma

An experiment lasting 45 days was performed on 125 Hybro broilers divided into five groups. All compounds for broiler feed mixes used in the experiment contained 0.15 mg Se/kg, in the form of sodium selenite. The control group (K-group) of broilers was fed mixes without added organic selenium, and the experimental groups with mixes to which selenium, in the form of selenized-yeast, was added in quantities of 2, 5, 10, or 15 mg/kg. Selenized yeast (ICN - Gaienika) was obtained from beer yeast and contained 1.51, or 1.45 mg/g total, or organically bound selenium. At the beginning of the fattening period, GSH-Px plasma activity in broilers of the K-group ranged around 16.55 ?kat/L, while GSH-Px plasma activity in broilers of experimental groups was statistically significantly higher, but without any major differences among the individual groups (on the average 25.53fjkat/L). In the blood plasma of K-group, GSH-Px activity dropped already in the second week of life and was maintained at a relatively constant level (about 10 ?kat/L) until the end of the experiment. The same phenomenon was observed in the experimental groups, but the trend of declining GSH-Px activity in blood plasma was more expressed, and, contrary to the control group, was expressed also in the later phases of the experiment. In the 3rd week of the fattening period, GSH-Px plasma activity in broilers of the control and experimental groups was relatively equal, and then the plasma activity of GSH-Px in broilers of the experimental groups decreased, but there were no major differences among the individual groups.

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

EFFECT OF DIETARY LEVELS OF SODIUM AND POTASSIUM ON GROWTH AND ON CONCENTRATIONS IN BLOOD PLASMA AND TISSUES OF WHITE RAT

1950 ◽  
Vol 162 (1) ◽  
pp. 182-188 ◽  
Author(s):  
J. H. Meyer ◽  
R. R. Grunert ◽  
Marie T. Zepplin ◽  
R. H. Grummer ◽  
G. Bohstedt ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Sodium And Potassium ◽  
In Blood Plasma

Two SPRi biosensors for the determination of cathepsin S in blood plasma

Talanta ◽  
2021 ◽  
Vol 225 ◽  
pp. 121900
Author(s):  
Lukasz Oldak ◽  
Anna Sankiewicz ◽  
Beata Żelazowska-Rutkowska ◽  
Bogdan Cylwik ◽  
Zenon Lukaszewski ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cathepsin S ◽  
In Blood Plasma

scholarly journals DETERMINATION OF TRYPSIN INHIBITOR IN BLOOD PLASMA

1953 ◽  
Vol 204 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Shirley F. McCann ◽  
M. Laskowski
Keyword(s):  
Blood Plasma ◽  
Trypsin Inhibitor ◽  
In Blood Plasma

scholarly journals The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

2021 ◽  
Author(s):  
Jacek Wawrzykowski ◽  
Monika Jamioł ◽  
Wioleta Mojsym ◽  
Marta Kankofer
Keyword(s):  
Blood Plasma ◽  
Antioxidative Enzymes ◽  
Placental Development ◽  
Blood Samples ◽  
Bovine Placenta ◽  
Total Antioxidant ◽  
Tissue Homogenates ◽  
Further Development ◽  
General Profile ◽  
In Blood Plasma

AbstractPhysiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.

The convenient UPLS method for the determination of Ceftiofur in blood plasma

2020 ◽  
Author(s):  
Artem V. Baklykov ◽  
Konstantin A. Chistiakov ◽  
Dmitry S. Kopchuk ◽  
Grigory V. Zyryanov ◽  
Gennady L. Rusinov ◽  
...  
Keyword(s):  
Blood Plasma ◽  
In Blood Plasma

scholarly journals AB0049 RELATIONSHIP OF RHEUMATOID FACTOR AND XANTHINE OXIDOREDUCTASE ENZYMATIC CONSTITUENTS IN SEVERAL BLOOD COMPARTMENTS OF RHEUMATOID ARTHRITIC PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1057.1-1057
Author(s):  
S. Bedina ◽  
A. Trofimenko ◽  
E. Mozgovaya ◽  
M. Mamus ◽  
S. Spitsina
Keyword(s):  
Blood Plasma ◽  
Rheumatoid Factor ◽  
Xanthine Oxidase ◽  
Xanthine Dehydrogenase ◽  
Enzymatic Activities ◽  
Systemic Autoimmune Disease ◽  
Ros Generation ◽  
Reference Ranges ◽  
Xanthine Oxidoreductase ◽  
In Blood Plasma

Background:Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by the presence of rheumatoid factor (RF) and anticitrulline autoantibodies. Recent evidences suggest that impairment of neutrophil extracellular traps (NETs) could exert substantial influence on RA pathogenesis. The production of NETs depends heavily on the ROS generation. One of its mechanisms is xanthine oxidoreductase (XOR) mediated degradation of purine metabolites. Analysis of pro-oxidant activity of the enzymatic complex XOR and its constituents, xanthine oxidase (XO) and xanthine dehydrogenase (XDG), is an issue of considerable interest in this context.Objectives:Evaluation of XO and XDG activities in RF-positive and RF-negative RA using both plasma and lysed lymphocyte samples.Methods:The research was carried out in agreement with the WMA Declaration of Helsinki principles. Diagnosis of RA had been verified using ACR/EULAR 2010 criteria. Enzymatic activities in plasma and lymphocytes were measured spectrophotometrically and expressed as nmol/min/ml. Enzymatic activities in lymphocytes were also normalized to 1×107 cells/ml. Statististical tests were selected in line with common guidelines. Differences were considered significant when p<0.05. Reference ranges were calculated as means ±2SD.Results:75 adult RA patients (52 females and 23 males, mean age 43.9±0.97 years, mean disease duration 8.5±0.3 years) from the rheumatology unit of Volgograd Clinical Emergency Hospital #25 as well as 35 healthy controls were included in the study. RF-positive RA and RF-negative RA were observed in 49 (65.3%) and 26 (34.7%) patients, respectively. Reference ranges for plasma and lymphocyte XO activities were 2.60-3.96 and 14.2-27.8 nmol/min/ml, respectively. Similar ranges for XDG activities were 4.49-5.93 and 22.5-40.7 nmol/min/ml, respectively. Enzymatic profile of RA patients is characterized by significantly increased XO activity in plasma and decreased XO and XDG activities in lymphocytes (р<0.001). XO activity is increased (p<0.001), XDG activity is decreased (p<0.001) in blood plasma of patients with RF-negative RA, while the activity of both enzymes is decreased in lymphocytes (p<0.001). XO activity (p<0.001) and XDG activity (p<0.05) is increased in blood plasma, XO activity and XDG activity are decreased (p<0.001) in lymphocytes of patients with RF-positive RA. Plasma XO and XDG activities are also higher, and lymphocyte XO and XDG activities are lower in patients with RF-positive RA than in patients with RF-negative RA (р<0.001).Conclusion:Our study revealed the relationship between enzyme parameters and rheumatoid factor presence. More pronounced changes in the enzyme activities were observed in patients with RF-positive RA. These results demonstrate that activation of the xanthine oxidase/xanthine dehydrogenase enzyme complex is an substantial factor of induction and continuation of the autoimmune rheumatoid inflammation.Disclosure of Interests:None declared

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