scholarly journals An Assessment of Wound Healing Potential ofArgyreia speciosaLeaves

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kuldeep Singh Yadav ◽  
Narayan Prasad Yadav ◽  
Bindu Rawat ◽  
Vineet Kumar Rai ◽  
Karuna Shanker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Potential ◽  
Breaking Strength ◽  
Present Report ◽  
Scientific Evidence ◽  
Water Extract ◽  
Wound Contraction ◽  
North India ◽  
Hydroxyproline Content ◽  
Traditional Use

In North India, poultice of young unfolded leaves ofArgyreia speciosaLinn. (Convolvulaceae) is used for healing wounds. In order to find scientific evidence for the traditional utilization of leaves ofA. speciosain wound healing, this investigation was carried out. A linear incision wound of about 3 cm in length and 2 mm in depth and circular excision wound of 177 mm2full thickness were made on the dorsal region of separate groups (n=5) of anesthetized Swiss albino mice. A simple ointment, developed by including ethanol, ethanol-water, and water extracts (10% each, separately) ofA. speciosa, was applied topically to mice once daily for 14 days after wounding. To evaluate the effect of each extract, wound contraction, epithelization period, wound breaking strength, and hydroxyproline content were determined. The water extract ofA. speciosashowed accelerated wound healing activity as evidenced by fast wound contraction (96.30±0.52%;P<0.01), rapid epithelization period (11.40±0.60days;P<0.001), greater wound breaking strength (376.56±21.16 g;P<0.001), and higher hydroxyproline content (16.49±1.12 mg/g;P<0.05) of granulation tissue. The present report supports the traditional use ofArgyreia speciosaleaves for wound healing and signify its relevant therapeutic potential.

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

scholarly journals In Vivo Wound Healing and Anti-Inflammatory Activities of Leaf Latex of Aloe megalacantha Baker (Xanthorrhoeaceae)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Leake Gebremeskel ◽  
Dayananda Bhoumik ◽  
Gereziher Geremedhin Sibhat ◽  
Kald Beshir Tuem
Keyword(s):  
Wound Healing ◽  
Breaking Strength ◽  
Negative Control ◽  
Wound Contraction ◽  
Traditional Use ◽  
Ointment Base ◽  
Healing Agent ◽  
Anti Inflammatory ◽  
Treatment Of Wounds

Background. Aloe megalacantha Baker (Xanthorrhoeaceae) is one of the Aloe species widely distributed in Ethiopia. The leaf latex of the plant is used for treatment of wounds, inflammation, and other multiple ailments in Ethiopian traditional medicine. Purpose. The aim of this study was to evaluate in vivo wound healing and anti-inflammatory activities of the leaf latex of Aloe megalacantha in mice. Methods. The wound healing activity of the leaf latex of the plant was studied topically by incorporating the latex in simple ointment base in a concentration of 5% (w/w) and 10% (w/w) using excision and incision models. In these models, wound contraction, period of epithelialization, and breaking strength of the wounded skin were determined. Carrageenan induced inflammation of paw model was also used to assess the anti-inflammatory activity of the leaf latex at doses of 200 mg/kg, 400mg/kg, and 600 mg/kg. The level of inflammation suppressions were measured at 1, 2, 3, and 4 hrs after carrageenan injection, and then the percentages of inflammation inhibition were computed as compared with the negative control. Result. In both wound models, mice treated with 5% (w/w) and 10% (w/w) latex ointment showed a significant (p<0.05) increment in the rate of wound contraction, reduction in epithelialization time, and higher skin breaking strength. Besides, the latex also exhibited a dose-dependent significant (p<0.05) reductions of inflammation as compared to negative control groups. Conclusion. The overall results of this study demonstrate that the leaf latex of A. megalacantha possesses wound healing and anti-inflammatory activities which can scientifically substantiate the traditional use of the plant as a wound healing agent.

scholarly journals The Impairment of Wound Healing Process is Correlated With Abnormalities of TNF-α Production by Peritoneal Exudate Cells in Obstructive Jaundiced Rats

HPB Surgery ◽  
2000 ◽  
Vol 11 (5) ◽  
pp. 311-318 ◽  
Author(s):  
Janusz Dawiskiba ◽  
Danuta Kwiatkowska ◽  
Michał Zimecki ◽  
Pawel Kornafel ◽  
Wanda Tyran ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Endothelial Cell ◽  
Breaking Strength ◽  
Healing Process ◽  
Skin Wound ◽  
Endothelial Cell Proliferation ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tnf Α

The wound healing process and production of tumour necrosis factor alpha (TNF-α) by peritoneal cells of 7-day and 14-day obstructive jaundice (OJ) and sham-operated rats were investigated. In the study the skin wound breaking strength was measured, In addition such histological and biochemical parameters as fibroblast and endothelial cell proliferation, inflammatory cell infiltration and hydroxyproline content were evaluated in polyurethane sponge discs implanted subcutaneously into rats. TNF-α production by peritoneal exudate cells (PEC), both spontaneous and lipopolysaccharide (LPS)- induced was determined by a bioassay. In OJ rats the process of both early as well as late phase of healing was impaired. The breaking strength of skin wound was decreased, the fibroblast and endothelial cell proliferation and collagen deposition, as well as hydroxyproline content were diminished. In 7 day OJ the numbers of inflammatory cells in the implants were lowered with a subsequent slight increase on day 14 of OJ. The spontaneous and LPS induced TNF- α production by PEC were significantly higher in 7 day OJ as compared with sham-operated controls. On day 14 of OJ the LPS-induced TNF-α level was, in contrast, much lower and did not differ much from the spontaneous TNF-α production. We conclude that the impairment of wound healing in OJ results from disturbances in functioning of the immune system caused by systemic endotoxaemia.

scholarly journals Wound Healing Activity of Topical Application Forms Based on Ayurveda

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Hema Sharma Datta ◽  
Shankar Kumar Mitra ◽  
Bhushan Patwardhan
Keyword(s):  
Wound Healing ◽  
Mechanical Strength ◽  
Seed Oil ◽  
Breaking Strength ◽  
Wound Contraction ◽  
Collagen Content ◽  
Tissue Cell ◽  
Cell Regeneration ◽  
Flax Seed ◽  
Shorea Robusta

The traditional Indian medicine—Ayurveda, describes various herbs, fats, oils and minerals with anti-aging as well as wound healing properties. With aging, numerous changes occur in skin, including decrease in tissue cell regeneration, decrease in collagen content, loss of skin elasticity and mechanical strength. We prepared five topical anti-aging formulations using cow ghee, flax seed oil,Phyllanthus emblicafruits,Shorea robustaresin,Yashada bhasmaas study materials. For preliminary efficacy evaluation of the anti-aging activity we chose excision and incision wound healing animal models and studied the parameters including wound contraction, collagen content and skin breaking strength which in turn is indicative of the tissue cell regeneration capacity, collagenation capacity and mechanical strength of skin. The group treated with the formulations containingYashada bhasmaalong withShorea robustaresin and flax seed oil showed significantly better wound contraction (P < .01), higher collagen content (P < .05) and better skin breaking strength (P < .01) as compared to control group; thus proposing them to be effective prospective anti-aging formulations.

scholarly journals Evaluation of the Wound-Healing Activity of Ethanolic Extract ofMorinda citrifoliaL. Leaf

2009 ◽  
Vol 6 (3) ◽  
pp. 351-356 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Steve Sandiford ◽  
Anderson Maxwell
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Ethanol Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Tissue Weight ◽  
Therapeutic Benefits ◽  
Wound Healing Activity

Morinda citrifoliaL. (noni) is one of the most important traditional Polynesian medicinal plants. The primary indigenous use of this plant appears to be of the leaves, as a topical treatment for wound healing. The ethanol extract of noni leaves (150 mg kg−1day−1) was used to evaluate the wound-healing activity on rats, using excision and dead space wound models. Animals were randomly divided into two groups of six for each model. Test group animals in each model were treated with the ethanol extract of noni orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, time until complete epithelialization, granulation tissue weight and hydoxyproline content. On day 11, the extract-treated animals exhibited 71% reduction in the wound area when compared with controls which exhibited 57%. The granulation tissue weight and hydroxyproline content in the dead space wounds were also increased significantly in noni-treated animals compared with controls (P< 0.002). Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content and histological characteristics suggest that noni leaf extract may have therapeutic benefits in wound healing.

scholarly journals Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Huihui Liu ◽  
Shaohui Lin ◽  
Dan Xiao ◽  
Xiao Zheng ◽  
Yan Gu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Histopathological Examination ◽  
Wound Contraction ◽  
Control Group ◽  
Traditional Use ◽  
Dragon’S Blood ◽  
Group I ◽  
Resina Draconis

Resina Draconis(RD) is a type of dragon's blood resin obtained fromDracaena cochinchinensis(Lour.) S.C. Chen (Yunnan, China). It has been used as a medicine since ancient times by many cultures. The ethanolic extract ofResina Draconis(RDEE) was evaluated for its wound-healing activity using excision and incision wound models in rats. Group I, the control group, was treated with ointment base. Group II, which served as a reference standard, was treated with moist exposed burn ointment (MEBO). Group III was treated with RDEE. The parameters observed were percentage of wound contraction, epithelialization period, tensile strength, histopathological studies, microvessel density (MVD), and the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). The group treated with RDEE showed significantly better wound contraction and better skin-breaking strength as compared with the control group. The results of histopathological examination, MVD, and the expression levels of growth factors supported the outcome of the wound models as well. The present study provided a scientific rationale for the traditional use of RD in the management of wounds.

scholarly journals In-vivo Evaluation of the Wound Healing Activity of the Sesamum Indicum L. Seed Extract in Novel Ethosomal Vesicular System

2018 ◽  
Vol 8 (5) ◽  
pp. 411-420 ◽  
Author(s):  
Sachin B Somwanshi ◽  
Shivanand N Hiremath
Keyword(s):  
Wound Healing ◽  
Breaking Strength ◽  
Wistar Rat ◽  
Treated Group ◽  
Sesamum Indicum ◽  
Seed Extract ◽  
Wound Contraction ◽  
In Vivo Evaluation ◽  
Wound Healing Activity

Aim: To establish the wound healing activity of Sesamum indicum L. of seed extract in novel ethosomal vesicles. Methods: The ethosomal vesicles were formulated with prepared seed extract of Sesamum indicum L. by solvent evaporation method and characterized it. The optimized ethosomal vesicles then incorporated into gel base for further in vivo study in wistar rat. The evaluation of the wound healing activity was performed by using two models i.e. incision and excision models. In excision model percentage wound contraction and period of epithelialization were established for both the extracts. In incision model the parameter which was carried out was breaking strength of wounded skin. Results: The results revealed that the percentage wound contraction, period of epithelialization in excision model was enhanced than that of other groups and tensile strength of skin in incision model was similar to that of standard treated group. Keywords: Sesamum indicum L., Wound Healing, Ethosomal gel.

IN VIVO EVALUATION OF WOUND HEALING AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF ROOTS OF CENTAUREA AFRICANA (L.) IN TOPICAL FORMULATION

2016 ◽  
Vol 10 (1) ◽  
pp. 341 ◽  
Author(s):  
Mohamed Zaouani ◽  
Arezki Bitam ◽  
Ahcen Baz ◽  
Yasmine Benali ◽  
Meriem Hind Ben-mahdi
Keyword(s):  
Wound Healing ◽  
Methanolic Extract ◽  
Wound Contraction ◽  
Inflammatory Activity ◽  
Hydroxyproline Content ◽  
Topical Formulation ◽  
In Vivo Evaluation ◽  
Anti Inflammatory ◽  
Wound Healing Activity

ABSTRACTObjective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.

scholarly journals Evaluation of polyherbal ointment for wound healing activity in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 26-31
Author(s):  
Shilpa S. Kolhe
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Breaking Strength ◽  
Skin Irritation ◽  
Wound Contraction ◽  
Alcoholic Extract ◽  
Polyherbal Formulation ◽  
Wound Healing Activity

The aim of present study to formulate, evaluate and to check the wound healing activity of newly prepared polyherbal formulation (ointment) .Herbal ointment containing hydrochloric extracts of plants Psorolea corylifalia, Achryanthes aspera ,was formulated as ointment and the hydro alcoholic extract was prepared by maceration method. These Formulations were evaluated for the following parameters: pH, Spread ability, grittiness, skin irritation study, stability. The wound healing activity is assessed by the rate of wound contraction, Period of epithelisation and skin breaking strength. Keywords: Herbal Ointment, Wound Healing Activity.

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