scholarly journals Nicotine-Cadmium Interaction Alters Exploratory Motor Function and Increased Anxiety in Adult Male Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Duyilemi Chris Ajonijebu ◽  
Philip Adeyemi Adeniyi ◽  
Adeshina Oloruntoba Adekeye ◽  
Babawale Peter Olatunji ◽  
Azeez Olakunle Ishola ◽  
...  
Keyword(s):  
Body Weight ◽  
Motor Function ◽  
Adult Male ◽  
Chronic Phase ◽  
Average Weight ◽  
Male Mice ◽  
Serum Electrolytes ◽  
Cadmium Treatment ◽  
Behavioural Tests

In this study we evaluated the time dependence in cadmium-nicotine interaction and its effect on motor function, anxiety linked behavioural changes, serum electrolytes, and weight after acute and chronic treatment in adult male mice. Animals were separated randomly into four groups of n = 6 animals each. Treatment was done with nicotine, cadmium, or nicotine-cadmium for 21 days. A fourth group received normal saline for the same duration (control). Average weight was determined at 7-day interval for the acute (D1-D7) and chronic (D7-D21) treatment phases. Similarly, the behavioural tests for exploratory motor function (open field test) and anxiety were evaluated. Serum electrolytes were measured after the chronic phase. Nicotine, cadmium, and nicotine-cadmium treatments caused no significant change in body weight after the acute phase while cadmium-nicotine and cadmium caused a decline in weight after the chronic phase. This suggests the role of cadmium in the weight loss observed in tobacco smoke users. Both nicotine and cadmium raised serum Ca2+ concentration and had no significant effect on K+ ion when compared with the control. In addition, nicotine-cadmium treatment increased bioaccumulation of Cd2+ in the serum which corresponded to a decrease in body weight, motor function, and an increase in anxiety.

The possible protective role of curcumin on the toxic effect of nicotine in the lung of adult male mice

2012 ◽  
Vol 35 (4) ◽  
pp. 805-811
Author(s):  
Dorria A.M. Zaghloul ◽  
Esam Salah Kamel ◽  
Hekmat O. Abd el-Aziz ◽  
Mohammed A. Mahmoud
Keyword(s):  
Toxic Effect ◽  
Adult Male ◽  
Protective Role ◽  
Male Mice

On the role of corticosterone in behavioral disorders, microbiota composition alteration and neuroimmune response in adult male mice subjected to maternal separation stress

2019 ◽  
Vol 66 ◽  
pp. 242-250 ◽  
Author(s):  
Hossein Amini-Khoei ◽  
Elaheh Haghani-Samani ◽  
Masoumeh Beigi ◽  
Amin Soltani ◽  
Gholam Reza Mobini ◽  
...  
Keyword(s):  
Behavioral Disorders ◽  
Adult Male ◽  
Maternal Separation ◽  
Microbiota Composition ◽  
Male Mice

scholarly journals Testes and body weight alteration related cardamom extract administration in mice

2019 ◽  
Vol 3 (1) ◽  
pp. 7
Author(s):  
Tyagita Hartady ◽  
Arvia Nisrina Praditha ◽  
Nabila Ayu Faza ◽  
Rini Widyastuti
Keyword(s):  
Body Weight ◽  
Mus Musculus ◽  
Point Of View ◽  
Average Weight

Cardamom is known as a plant with millions of benefits and it is known to contain aphrodisiac substances. The role of cardamom as an aphrodisiac need to be studied more deeply from the scientific point of view. The experimental object is a 3-month-old male white mice (<em>Mus musculus albinus</em>) with an average weight of about 30-35 grams. After being acclimatized for approximately 2 weeks, 35 mice were divided into 3 groups based on the dose of cardamom extract. At the end of the experiment, the mice will be sacrificed and then the testes were weighed and cauda epididymis were isolated to collect the sperm. Our findings suggested that the dose of administration has important role in affecting the weight of testes and bodies of mice. However, further studies on sperm profiles and optimal administration doses of cardamom extract are highly recommended.

scholarly journals CCR3 deficiency is associated with increased osteoclast activity and reduced cortical bone volume in adult male mice

2020 ◽  
pp. jbc.RA120.015571
Author(s):  
Sara Rosendahl ◽  
Rima Sulniute ◽  
Michaela Eklund ◽  
Cecilia Koskinen Holm ◽  
Marcus J. O. Johansson ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cortical Bone ◽  
Bone Remodeling ◽  
Adult Male ◽  
Chemokine Receptors ◽  
Osteoclast Formation ◽  
Male Mice ◽  
Primary Mouse ◽  
Deficient Mice

Increasing evidence emphasizes the importance of chemokines and chemokine receptors as regulators of bone remodeling. The C-C chemokine receptor 3 (CCR3) is dramatically up-regulated during osteoclastogenesis but the role of CCR3 in osteoclast formation and bone remodeling in adult mice is unknown. Herein, we used bone marrow macrophages (BMM) derived from adult male CCR3-proficient and -deficient mice to study the role of CCR3 in osteoclast formation and activity. CCR3 deficiency was associated with formation of giant hypernucleated osteoclasts, enhanced bone resorption when cultured on bone slices and altered mRNA expression of related chemokine receptors and ligands. Additionally, primary mouse calvarial osteoblasts isolated from CCR3-deficient mice showed increased mRNA expression of the osteoclast activator related gene, receptor activator of nuclear factor kappa-B ligand (Rankl), and osteoblast differentiation associated genes. Micro-computed tomography analyses of femurs from CCR3-deficient mice revealed a bone phenotype that entailed less cortical thickness and volume. Consistent with our in vitro studies, the number of osteoclasts did not differ between the genotypes in vivo. Moreover, an increased endo-cortical osteoid mineralization rate and higher trabecular and cortical bone formation rate was displayed in CCR3-deficient mice. Collectively, our data show that CCR3 deficiency influences osteoblast and osteoclast differentiation and that it is associated with thinner cortical bone in adult male mice.

Role of the ovary in sexual differentiation of lactotrophs and somatotrophs in the mouse adenohypophysis: a stereological morphometric study by electron microscopy

1983 ◽  
Vol 99 (3) ◽  
pp. 355-NP ◽  
Author(s):  
F. Sasaki ◽  
M. Sano
Keyword(s):  
Adult Male ◽  
Sexual Differentiation ◽  
Morphometric Study ◽  
Normal Male ◽  
Corpora Lutea ◽  
Male Mice ◽  
Female Mice ◽  
Pituitary Glands ◽  
Normal Males

To study the effect of the ovary on sexual differentiation of somatotrophs and lactotrophs, the anterior pituitary glands of castrated adult male mice which had received an ovarian transplant during postnatal development were studied using a stereological morphometric technique with an electron microscope. In adult male mice which were castrated neonatally and given ovarian transplants at the age of puberty (NCT-males), the ovaries contained follicles and corpora lutea. The percentages (∼40) and numbers (∼2 × 105) of lactotrophs were similar in normal dioestrous females and NCT-males, but were higher than the percentage (9·3) and number (4·6 × 104) in normal males. Ovarian grafts in adult male mice which were simultaneously castrated and given an ovarian transplant just before puberty (PCT-males) contained numerous follicles of various sizes but no corpora lutea. The percentage (46·8) and number (3·9 × 105) of lactotrophs were greater in these mice than in dioestrous females. The percentage of somatotrophs in NCT-males (34·7) was less than in normal males (52·6), but was similar to that in dioestrous female mice (37·4). The percentage of somatotrophs in PCT-males (27·4) was less than in normal male and dioestrous female mice. These data indicate that lactotrophs and somatotrophs differentiate to the female phenotype when a cyclically functional ovary is present after puberty.

scholarly journals Dysfunction of EAAT3 Enhances LPS-induced Postoperative Cognitive Dysfunction

2021 ◽  
Author(s):  
Xiaoyan Wang ◽  
Yulong Ma ◽  
Aisheng Hou ◽  
Yuxiang Song ◽  
Xin Sui ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Learning And Memory ◽  
Cognitive Dysfunction ◽  
Adult Male ◽  
Excitatory Amino Acid ◽  
Postoperative Cognitive Dysfunction ◽  
Male Mice ◽  
Membrane Protein Expression

Abstract Background: Studies have shown that excitatory amino acid transporter 3 (EAAT3) function inhibition is related to several neurodegenerative diseases. Our previous studies also found that the EAAT3 function is intimately linked to learning and memory. In this study, we examined the role of EAAT3 in postoperative cognitive dysfunction (POCD) and explored the potential benefit of riluzole against POCD. Methods: We measured EAAT3 protein expression in hippocampus of male mice at different ages. Next, we established a recombinant adeno-associated viral (rAAV)-mediated shRNA to knockdown EAAT3 expression in the hippocampus of adult male mice. And then the mice received 2μg of lipopolysaccharide (LPS) intracerebroventricular microinjection to construct the POCD model. In addition, we intraperitoneally injected 4mg/kg of riluzole 2 days before LPS microinjection for consecutive 3 days in elderly male mice. Cognitive function was assessed using a Morris water maze 24h after LPS microinjection. Animal behavioral tests, as well as pathological and biochemical assays, were performed to clarify the role of EAAT3 function in POCD and evaluate the effect of activation of EAAT3 function by riluzole. Results: We found that the expression of EAAT3 was significantly decreased in old mice and EAAT3 knockdown in hippocampus aggravated LPS-induced learning and memory deficits in adult male mice. LPS significantly inhibited hippocampal EAAT3 membrane protein expression and GluA1 protein phosphorylation level in adult male mice. Moreover, riluzole pretreatment significantly increased hippocampal EAAT3 membrane protein expression and ameliorated LPS-induced cognitive impairment in old male mice. Conclusions: Our results demonstrated that the dysfunction of EAAT3 is an important risk factor for POCD susceptibility and riluzole may be a promising strategy for prevention and treating of POCD in the elderly people.

scholarly journals Morphometry of indian roofed turtle, Pangshura tecta (Gray 1831) in Bangladesh

2015 ◽  
Vol 41 (2) ◽  
pp. 207-215
Author(s):  
Md Lokman Hossain ◽  
Shorab Uddin Sarker ◽  
Noor Jahan Sarker
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
The Body ◽  
Morphometric Study ◽  
Average Weight ◽  
The Mean

Morphometric study was conducted on Indian roofed turtle, Pangshura tecta, between January 1997 and December 2000 in several district of Bangladesh. The mean weight of adult male was 92.6 ± 13.3 g and of female 441.1 ± 185.4 g. The length and width of carapace as well as those of the plastron and the height of the shell varied with the body weight of the turtle. The percentage of hard parts of the body weight of P. tecta was 35.8 ± 2.5 %, whereas percentage of soft parts of the body weight was 64.2 ± 2.5 %. The average weight of the female P. tecta was 4.8 times higher than that of the male.Bangladesh J. Zool. 41(2): 207-215, 2013

Effect of Paracetamol on Sperm Biological Parameters and Testosterone Level in Albino Male Mice

2021 ◽  
pp. 51-57
Author(s):  
Habiba Eljaafari ◽  
Zainab EL Mabrouk ◽  
Marwan Rashrash
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Testosterone Level ◽  
Sperm Morphology ◽  
Sperm Parameters ◽  
Male Mice ◽  
Once Daily ◽  
High Doses ◽  
Fertilizing Capability ◽  
Different Doses

Backgrounds and objectives. The wide use of paracetamol at high doses was found to alter sperm parameters especially sperm morphology, and thus its fertilizing capability. Therefore, the present study was designed to use different doses of paracetamol to identify its effect on sperm parameters and testosterone levels in adult male mice. Methods. Forty adult male albino mice were divided into four equal groups, the first group injected with distilled water, the three treated groups injected with different doses of paracetamol (20, 40, 80 mg/kg body weight /day) over a period of 42 days. All doses were given once daily via intraperitoneal injection. Results. The results showed that paracetamol causes a significant decrease in body weight, non-significance effect on sperm parameters at doses of 20 and 40 mg/kg, while it led to a significant effect on sperm parameters at a dose of 80 mg/kg. Also, there was no difference in testosterone level between control and the treated groups (20 and 40mg/kg). But it showed a significant decrease in testosterone level at dose 80 mg/kg treated groups. Conclusion. It is considered safe to use paracetamol at doses 20 and 40 mg/kg but the dose 80 mg/kg has adverse effects on sperm parameters and testosterone level.

scholarly journals Endocrine disruptor bisphenol A is implicated in urinary voiding dysfunction in male mice

2018 ◽  
Vol 315 (5) ◽  
pp. F1208-F1216 ◽  
Author(s):  
Tristan M. Nicholson ◽  
Jalissa L. Nguyen ◽  
Glen E. Leverson ◽  
Julia A. Taylor ◽  
Frederick S. vom Saal ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Adult Male ◽  
Endocrine Disruptor ◽  
Voiding Dysfunction ◽  
Animal Experiments ◽  
Bladder Volume ◽  
Male Mice ◽  
Prostate Enlargement ◽  
Urinary Tract Symptoms

Estrogens, acting synergistically with androgens, are known from animal experiments to be important in lower urinary tract symptoms (LUTS) and benign prostate enlargement. Human exposure to environmental estrogens occurs throughout the life span, but the urologic health risks in men are largely unknown. Bisphenol A (BPA) is an endocrine disruptor implicated in male urogenital malformations. Given the role of estrogens in male LUTS, we studied the effects of BPA administered in combination with testosterone (T) on the urinary voiding behavior of adult male mice. Adult male mice underwent subcutaneous implantation with slow-release pellets of 25 mg BPA or 2.5 mg estradiol-17β (E2), plus 25 mg T, and were compared with untreated (UNT) mice that underwent sham surgery. We studied urinary voiding behavior noninvasively for 1 mo before treatment and for 4 mo after treatment. After euthanasia, we evaluated bladder volume and mass. Mice treated with T+BPA had increased bladder volume ( P < 0.05) and mass ( P < 0.01) compared with UNT mice. After 4 mo of treatment with T+BPA, three of five mice developed voiding dysfunction in the form of droplet voiding or an intermediate pattern of voiding different from both UNT and T+E2-treated mice. Treatment of male mice with BPA or estradiol induces voiding dysfunction that manifests at later time points, implicating the endocrine disruptor, BPA, as a contributor to male LUTS.

Sign in / Sign up

Export Citation Format

Share Document