scholarly journals The Establishment of Metabolic Syndrome Model by Induction of Fructose Drinking Water in Male Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Norshalizah Mamikutty ◽  
Zar Chi Thent ◽  
Shaiful Ridzwan Sapri ◽  
Natasya Nadia Sahruddin ◽  
Mohd Rafizul Mohd Yusof ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Drinking Water ◽  
Rat Model ◽  
Wistar Rats ◽  
Calorie Intake ◽  
The Metabolic Syndrome ◽  
Male Wistar Rats ◽  
Metabolic Syndrome Model ◽  
One Way Anova ◽  
Food And Fluid Intake

Background. Metabolic syndrome can be caused by modification of diet by means of consumption of high carbohydrate and high fat diet such as fructose.Aims. To develop a metabolic syndrome rat model by induction of fructose drinking water (FDW) in male Wistar rats.Methods. Eighteen male Wistar rats were fed with FDW 20% and FDW 25% for a duration of eight weeks. The physiological changes with regard to food and fluid intake, as well as calorie intake, were measured. The metabolic changes such as obesity, dyslipidaemia, hypertension, and hyperglycaemia were determined. Data was presented in mean ± SEM subjected to one-way ANOVA.Results. Male Wistar rats fed with FDW 20% for eight weeks developed significant higher obesity parameters compared to those fed with FDW 25%. There was hypertrophy of adipocytes in F20 and F25. There were also systolic hypertension, hypertriglyceridemia, and hyperglycemia in both groups.Conclusion. We conclude that the metabolic syndrome rat model is best established with the induction of FDW 20% for eight weeks. This was evident in the form of higher obesity parameter which caused the development of the metabolic syndrome.

scholarly journals Fructose-Drinking Water Induced Nonalcoholic Fatty Liver Disease and Ultrastructural Alteration of Hepatocyte Mitochondria in Male Wistar Rat

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Norshalizah Mamikutty ◽  
Zar Chi Thent ◽  
Farihah Haji Suhaimi
Keyword(s):  
Metabolic Syndrome ◽  
Drinking Water ◽  
Liver Disease ◽  
Fatty Liver Disease ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Ultrastructural Changes ◽  
Nonalcoholic Fatty Liver ◽  
The Metabolic Syndrome ◽  
Male Wistar Rats

Background.Nonalcoholic fatty liver disease (NAFLD) is one of the complications of the metabolic syndrome. It encompasses a wide range of disease spectrum from simple steatosis to liver cirrhosis. Structural alteration of hepatic mitochondria might be involved in the pathogenesis of NAFLD.Aims.In the present study, we used a newly established model of fructose-induced metabolic syndrome in male Wistar rats in order to investigate the ultrastructural changes in hepatic mitochondria that occur with fructose consumption and their association with NAFLD pathogenesis.Methods.The concentration of fructose-drinking water (FDW) used in this study was 20%. Six male Wistar rats were supplemented with FDW 20% for eight weeks. Body composition and metabolic parameters were measured before and after 8 weeks of FDW 20%. Histomorphology of the liver was evaluated and ultrastructural changes of mitochondria were assessed with transmission electron micrograph.Results.After 8 weeks of fructose consumption, the animals developed several features of the metabolic syndrome. Moreover, fructose consumption led to the development of macrovesicular hepatic steatosis and mitochondrial ultrastructural changes, such as increase in mitochondrial size, disruption of the cristae, and reduction of matrix density.Conclusion.We conclude that in male Wistar rat 8-week consumption of FDW 20% leads to NAFLD likely via mitochondrial structural alteration.

scholarly journals MULTIPARTICULATE FLOATING DRUG DELIVERY SYSTEM OF ANAGLIPTIN: DESIGN AND OPTIMIZATION FOR ITS EFFICACY IN MANAGEMENT OF METABOLIC SYNDROME

2019 ◽  
pp. 171-181
Author(s):  
RAKESH V. MISHRA ◽  
SHASHIKANT N. DHOLE
Keyword(s):  
Metabolic Syndrome ◽  
Drug Release ◽  
Wistar Rats ◽  
Lag Time ◽  
Cafeteria Diet ◽  
High Density Lipoproteins ◽  
Eudragit Rs ◽  
The Metabolic Syndrome ◽  
Male Wistar Rats ◽  
Eudragit Rl

Objective: The present research aims to design and optimize gastroretentive floating pellets of anagliptin (a dipeptidyl peptidase-4 inhibitor), so as to reduce P-Glycoprotein (PGP)–mediated efflux in the intestine hence to improve oral bioavailability. Methods: The drug-containing core pellets were prepared by extrusion and spheronization process followed by subsequent coating with three successive layers i.e. Eudragit RS 100, sodium bicarbonate (NaHCO3): hydroxypropyl methylcellulose E5LV (HPMC E5LV) and Eudragit RL 100 using fluidized bed processor. A 3 level 3 factor box-behnken design was adopted to investigate the effect of Eudragit RS 100, NaHCO3: HPMC E5LVand Eudragit RL 100 on floating lag time and drug release at 10 h. Desirability function under numerical optimization technique was used to identify the optimum formulation. Results: The study reveals the significant effect of the amount of NaHCO3 and coating level of polymers on floating lag time and drug release. The optimum system could float within 4 min and exhibited more than 85% drug release in 10 h. The pharmacokinetic study conducted in male Wistar rats indicated 2.51 fold increase in relative bioavailability of optimized formulation compare to anagliptin drug. Formulated anagliptin pellets were evaluated in cafeteria diet-induced metabolic syndrome model in male Wistar rats. Anagliptin floating pellets treatment compared to cafeteria diet group significantly inhibited increase in body weight (238.79±2.52 g vs. 277.98±3.69 g, P<0.001), calorie intake (2283.99 kcal vs. 3086.05 kcal, P<0.05) and serum levels of total cholesterol (95.19±0.61 mg/dl vs. 110.04±1.31 mg/dl, P<0.01), triglycerides (96.12±1.25 mg/dl vs. 105.99±1.29 mg/dl, P<0.01) while high-density lipoproteins levels were improved (42.15±0.92 mg/dl vs. 30.92±0.77 mg/dl, P<0.01) indicated its hypophagic and anti-hyperlipidemic effects. Conclusion: The gastroretentive floating pellets of anagliptin was obtained and could be a promising technique to deliver anagliptin with improved bioavailability in the management of the metabolic syndrome.

scholarly journals Ameliorative activity of Adansonia digitata fruit on high sugar/high fat diet-simulated Metabolic Syndrome model in male Wistar rats

2020 ◽  
Vol 125 ◽  
pp. 109968
Author(s):  
Hayat Mohamed Suliman ◽  
Bashier Osman ◽  
Iman H. Abdoon ◽  
Amir Mustafa Saad ◽  
Hassan Khalid
Keyword(s):  
Metabolic Syndrome ◽  
High Fat Diet ◽  
Wistar Rats ◽  
High Fat ◽  
Adansonia Digitata ◽  
High Sugar ◽  
Male Wistar Rats ◽  
Metabolic Syndrome Model

Insulin-induced endothelin release and vasoreactivity in hypertriglyceridemic and hypertensive rats

1999 ◽  
Vol 277 (1) ◽  
pp. H399-H404 ◽  
Author(s):  
Pilar Nava ◽  
Verónica Guarner ◽  
Rosalinda Posadas ◽  
Israel Pérez ◽  
Guadalupe Baños
Keyword(s):  
Drinking Water ◽  
Receptor Antagonist ◽  
Wistar Rats ◽  
Receptor Blocker ◽  
Hypertensive Rats ◽  
Contractile Responses ◽  
Femoral Arteries ◽  
Male Wistar Rats

Insulin-elicited endothelin release in hypertriglyceridemic, hypertensive, hyperinsulinemic (HTG) rats was shown. Weanling male Wistar rats were given 30% sucrose in their drinking water for 20–24 wk. In vitro contractions of aorta and femoral arteries were elicited with 40 mM KCl. Endothelin release induced with KCl plus 50 μU/ml insulin resulted in increases in contractile responses: 41 ± 5.9 and 57 ± 6% for control and 65.5 ± 6 and 95 ± 9% for HTG aortas and femoral arteries, respectively. The endothelin ETB-receptor blocker BQ-788 decreased responses to KCl + insulin by 39 ± 8 and 53 ± 5% in control and 48 ± 13 and 79 ± 3.5% in HTG aortas and femoral arteries, respectively. The ETA-receptor antagonist PD-151242 inhibited these responses by 12 ± 10 and 1 ± 9% in control and by 51.5 ± 9 and 58.5 ± 1% in HTG aortas and femoral arteries, respectively. These results suggest that endothelin may contribute to the hypertension in this model.

scholarly journals Improvement of Cardiac Fibrosis Biomarkers through Inflammation Inhibition by Green Tea and Decaffeinated Light Roasted Green Coffee Extract Combination Administration in Metabolic Syndrome Rat Model

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 1013
Author(s):  
Mifetika Lukitasari ◽  
Mohammad Saifur Rohman ◽  
Dwi Adi Nugroho ◽  
Nila Aisyah Wahyuni ◽  
Mukhamad Nur Kholis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Syndrome ◽  
Green Tea ◽  
Rat Model ◽  
Cardiac Fibrosis ◽  
Significant Risk ◽  
Green Coffee ◽  
The Metabolic Syndrome ◽  
Tnf Α ◽  
Tgf Β1

Background: Metabolic syndrome is a significant risk factor for cardiovascular diseases. Green tea and green coffee extracts, antioxidant and anti-inflammatory agents may participate in metabolic syndrome-induced cardiac fibrosis alleviation. However, the effect of combination of those extracts still needs exploration. Therefore, this study investigated the effect of green tea and decaffeinated light roasted green coffee extracts and their combination in metabolic syndrome-induced cardiac fibrosis rats. Methods: Metabolic syndrome rat model was i1nduced through high-fat high sucrose diets feeding for 8 weeks and injection of low dose streptozotocin at the 2nd week. The metabolic syndrome rats were divided into 4 experimental groups metabolic syndrome rats (MS); metabolic syndrome rats treated with 300 mg/ kg b.w green tea extract (GT); metabolic syndrome rats treated with 200 mg/ kg b.w decaffeinated light roasted green coffee extract (GC); metabolic syndrome rats treated with the combination of the two extracts (CE); and a normal control (NC) group was added. Angiotensin 2 level was analyzed by ELISA method. Gene expression of NF-κB, TNF-α, IL-6, Tgf-β1, Rac-1, and α-sma were analyzed by touchdown polymerase chain reaction methods. Results: Metabolic syndrome rats treated with green tea and decaffeinated light roasted green coffee significantly decreased angiotensin-2 serum level and cardiac inflammation and fibrosis gene expression level (NF-κB, TNF-α, IL-6, Tgf-β1, Rac-1, and α-sma). More significant alleviation was observed in the combination group. Conclusion: This study suggested that combination of green tea and decaffeinated light roasted green coffee extracts showed better improvement in metabolic syndrome-induced cardiac fibrosis rat model compared to that of single extract administration through inflammation inhibition

scholarly journals Association between Dietary Habits, Shift Work, and the Metabolic Syndrome: The Korea Nurses’ Health Study

2020 ◽  
Vol 17 (20) ◽  
pp. 7697
Author(s):  
Heeja Jung ◽  
Hyunju Dan ◽  
Yanghee Pang ◽  
Bohye Kim ◽  
Hyunseon Jeong ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Shift Work ◽  
Dietary Habits ◽  
Public Health Problem ◽  
Health Study ◽  
Calorie Intake ◽  
Important Public Health Problem ◽  
Family History Of Diabetes ◽  
The Metabolic Syndrome ◽  
Factors Influencing

Metabolic syndrome (MetS) is an important public health problem, and unhealthy dietary habits and shift work are considered major factors that increase the prevalence of MetS. The purpose of this study was to examine whether dietary habits, alcohol drinking, and shift-working were associated with development of MetS in shift-working female nurses. This study analyzed cross-sectional survey data from the Korea Nurses’ Health Study (KNHS). Of the 1638 nurses, 403 participants were selected based on the propensity score matching method (PSM). These participants had either no or more than three MetS determinant factors. Analysis was conducted by using multivariable logistic regression to confirm the factors influencing MetS. The prevalence of MetS in this group (1638 participants) was 5.6% (92 participants). Consumption of over 50% of daily calorie intake after 7 p.m., consumption of carbonated drinks, family history of diabetes, and non-shift work were significant factors influencing MetS. Nurses are one of the at-risk groups for unhealthy dietary habits due to the nature of their work. Therefore, nurse managers should include regular dietary education for nurses and continue their policy efforts to resolve health problems that may arise in connection with nurses’ work.

scholarly journals The Effects of Tocotrienol on Bone Peptides in a Rat Model of Osteoporosis Induced by Metabolic Syndrome: The Possible Communication between Bone Cells

2019 ◽  
Vol 16 (18) ◽  
pp. 3313 ◽  
Author(s):  
Sok Kuan Wong ◽  
Kok-Yong Chin ◽  
Soelaiman Ima-Nirwana
Keyword(s):  
Metabolic Syndrome ◽  
Rat Model ◽  
Animal Studies ◽  
Bone Cells ◽  
Corn Oil ◽  
Fibroblast Growth Factor 23 ◽  
Positive Association ◽  
Bone Remodelling Process ◽  
Male Wistar Rats ◽  
Fgf 23

A positive association between metabolic syndrome (MetS) and osteoporosis has been demonstrated in previous animal studies. The mechanisms of MetS in orchestrating the bone remodelling process have traditionally focused on the interactions between mature osteoblasts and osteoclasts, while the role of osteocytes is unexplored. Our earlier studies demonstrated the bone-promoting effects of tocotrienol using a rat model of osteoporosis induced by MetS. This study aimed to investigate the expression of osteocyte-derived peptides in the bone of rats with MetS-induced osteoporosis treated with tocotrienol. Age-matched male Wistar rats (12-week-old; n = 42) were divided into seven experimental groups. Two groups served as the baseline and normal group, respectively. The other five groups were fed with a high-carbohydrate high-fat (HCHF) diet to induce MetS. The five groups of HCHF animals were treated with tocopherol-stripped corn oil (vehicle), annatto tocotrienol (60 and 100 mg/kg), and palm tocotrienol (60 and 100 mg/kg) starting from week 8. At the end of the study, the rats were sacrificed and their right tibias were harvested. Protein was extracted from the metaphyseal region of the proximal right tibia and levels of bone peptides, including osteoprotegerin (OPG), soluble receptor activator of nuclear factor-kappa B ligand (sRANKL), sclerostin (SOST), Dickkopf-related protein 1 (DKK-1), fibroblast growth factor-23 (FGF-23), and parathyroid hormone (PTH), were measured. The vehicle-treated animals displayed higher levels of sRANKL, SOST, DKK-1, FGF-23, and PTH as compared to the normal animals. Oral supplementation of annatto and palm tocotrienol (60 and 100 mg/kg) reduced the levels of sRANKL and FGF-23 in the HCHF animals. Only 100 mg/kg annatto and palm tocotrienol lowered SOST and DKK-1 levels in the HCHF animals. In conclusion, tocotrienol exerts potential skeletal-promoting benefit by modulating the levels of osteocytes-derived bone-related peptides.

METFORMIN ADMINISTRATION TO A RAT MODEL OF THE METABOLIC SYNDROME

2004 ◽  
Vol 22 (Suppl. 1) ◽  
pp. S110
Author(s):  
C BN Ferreira ◽  
M LR Cesaretti ◽  
M Ginoza ◽  
M T Zaneila ◽  
A B Ribeiro ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Rat Model ◽  
The Metabolic Syndrome

W09-P-027 A new transgenic rat model of hepaticsteatosis and the metabolic syndrome

2005 ◽  
Vol 6 (1) ◽  
pp. 46
Author(s):  
V. Zídek ◽  
V. Landa ◽  
P. Mlejnek ◽  
N. Qi ◽  
J. Wang ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Rat Model ◽  
Transgenic Rat ◽  
The Metabolic Syndrome ◽  
Transgenic Rat Model
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