scholarly journals Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Shalini Singh ◽  
Sanamdeep Singh ◽  
Vrinda Bali ◽  
Lovleen Sharma ◽  
Jyoti Mangla
Keyword(s):  
Aspergillus Fumigatus ◽  
Textile Industry ◽  
Amylase Activity ◽  
Test Strain ◽  
Pomegranate Peel ◽  
Nutrient Salt ◽  
Amylase Production ◽  
Wet Processing ◽  
Initial Ph

The study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced.Aspergillus fumigatusNTCC1222 showed the highest amylase activity (164.1 U/mL) in secondary screening under SSF conditions and was selected for further studies. The test strain showed maximum amylase production (341.7 U/mL) and supernatant protein concentration (9.7 mg/mL) for incubation period (6 days), temperature (35°C), initial pH (6.0), nutrient salt solution as moistening agent, and beef extract as nitrogen source. Pomegranate peel produced maximum amylase activity, but wheat bran (only slightly lesser amylase activity as compared to that of pomegranate peel) was chosen for further studies, keeping in mind the seasonal availability of pomegranate peel. TLC confirmed the amylase produced to beα-type and 60 kDa was the molecular weight of the partially purified amylase. The enzyme showed maximum enzyme activity at pH 6.0, temperature of 55°C, and incubation time of 60 minutes. UV (616.0 U/mL) and chemical (814.2 U/mL) mutation enhanced amylase activity as compared to wild test strain. The study indicates thatAspergillus fumigatusNTCC1222 can be an important source of amylase and the crude enzyme, hence obtained, can be cost effectively applied in multiple sections of textile wet processing.

scholarly journals Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase fromPseudomonas stutzeriAS22

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hana Maalej ◽  
Hanen Ben Ayed ◽  
Olfa Ghorbel-Bellaaj ◽  
Moncef Nasri ◽  
Noomen Hmidet
Keyword(s):  
Culture Medium ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Potato Starch ◽  
Pseudomonas Stutzeri ◽  
Crude Enzyme Preparation ◽  
Amylase Production ◽  
Initial Ph ◽  
Very High

Amylase production and biochemical characterization of the crude enzyme preparation fromPseudomonas stutzeriAS22 were evaluated. The highestα-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crudeα-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that bothα-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.

scholarly journals Factors responsible for production of amylases from Aspergillus fumigatus Fresenius

2013 ◽  
pp. 11-20 ◽  
Author(s):  
Munira Akhter ◽  
Md Towhid Hossain ◽  
MN Anwar
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Fumigatus ◽  
Ammonium Nitrate ◽  
Amylase Activity ◽  
Soluble Starch ◽  
Culture Ph ◽  
Enzyme Substrate ◽  
Amylase Production ◽  
Initial Culture

Aspergillus fumigatus Fresenius showed maximum amylase production at 27°C with an initial culture pH of the medium 6.0 after 72 h. of incubation. One per cent soluble starch and 0.15% ammonium nitrate in the medium supported the highest amylase activity. During enzyme-substrate reaction maximum enzyme activity was observed at 50°C and pH 4.0 with 2% starch. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13402 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):11-20, 2008

Production of amylase by soil fungi and partial biochemical characterization of amylase of a selected strain (Aspergillus fumigatus Fresenius)

1993 ◽  
Vol 39 (7) ◽  
pp. 681-685 ◽  
Author(s):  
Claudia M. Domingues ◽  
Rosane M. Peralta
Keyword(s):  
Aspergillus Fumigatus ◽  
Soil Fungi ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Carbon Sources ◽  
Lignocellulosic Materials ◽  
Liquid Media ◽  
Maximal Yield ◽  
High Enzymatic Activity

Filamentous fungi from soil were screened for their ability to produce amylases in semisolid and liquid media with wheat bran. A selected strain identified as Aspergillus fumigatus Fresenius showed high enzymatic activity for α-amylase and glucoamylase. The maximal yield of these amylases was obtained when lignocellulosic materials were the carbon sources. The optimal pH and temperature were 6.0 and 50 °C, respectively, for both enzymes. α-Amylase activity was more thermostable than glucoamylase activity.Key words: amylolitic fungi, α-amylase, glucoamylase, Aspergillus fumigatus.

scholarly journals Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

2010 ◽  
Vol 53 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Arlem Nascimento de Oliveira ◽  
Luiz Antonio de Oliveira ◽  
Jerusa Souza Andrade
Keyword(s):  
Amylase Activity ◽  
Tween 80 ◽  
Inhibitory Effect ◽  
Partial Characterization ◽  
Exponential Growth Phase ◽  
Enzyme Preparations ◽  
Amylase Production ◽  
Ph Range ◽  
Triton X

Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.

scholarly journals Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus

2021 ◽  
Vol 22 (7) ◽  
pp. 3777
Author(s):  
Yong-Ho Choi ◽  
Sang-Cheol Jun ◽  
Min-Woo Lee ◽  
Jae-Hyuk Yu ◽  
Kwang-Soo Shin
Keyword(s):  
Aspergillus Fumigatus ◽  
Pathogenic Fungus ◽  
Hyphal Growth ◽  
Spore Wall ◽  
Mrna Levels ◽  
Chitin Synthesis ◽  
Helix Loop Helix ◽  
Growth Development ◽  
Opportunistic Pathogenic

The APSES family proteins are transcription factors (TFs) with a basic helix-loop-helix domain, known to regulate growth, development, secondary metabolism, and other biological processes in Aspergillus species. In the genome of the human opportunistic pathogenic fungus Aspergillus fumigatus, five genes predicted to encode APSES TFs are present. Here, we report the characterization of one of these genes, called mbsA (Afu7g05620). The deletion (Δ) of mbsA resulted in significantly decreased hyphal growth and asexual sporulation (conidiation), and lowered mRNA levels of the key conidiation genes abaA, brlA, and wetA. Moreover, ΔmbsA resulted in reduced spore germination rates, elevated sensitivity toward Nikkomycin Z, and significantly lowered transcripts levels of genes associated with chitin synthesis. The mbsA deletion also resulted in significantly reduced levels of proteins and transcripts of genes associated with the SakA MAP kinase pathway. Importantly, the cell wall hydrophobicity and architecture of the ΔmbsA asexual spores (conidia) were altered, notably lacking the rodlet layer on the surface of the ΔmbsA conidium. Comparative transcriptomic analyses revealed that the ΔmbsA mutant showed higher mRNA levels of gliotoxin (GT) biosynthetic genes, which was corroborated by elevated levels of GT production in the mutant. While the ΔmbsA mutant produced higher amount of GT, ΔmbsA strains showed reduced virulence in the murine model, likely due to the defective spore integrity. In summary, the putative APSES TF MbsA plays a multiple role in governing growth, development, spore wall architecture, GT production, and virulence, which may be associated with the attenuated SakA signaling pathway.

Cloning, Expression, and Enzyme Characterization of an Acid Heat-Stable Phytase from Aspergillus fumigatus WY-2

2007 ◽  
Vol 55 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Yan Wang ◽  
Xiaorong Gao ◽  
Qiao Su ◽  
Wei Wu ◽  
Lijia An
Keyword(s):  
Aspergillus Fumigatus ◽  
Enzyme Characterization ◽  
Heat Stable

Isolation, Identification and Degradation Characterization of Synthetic Dyes Degrading Strain

2012 ◽  
Vol 610-613 ◽  
pp. 3140-3143
Author(s):  
Hui Xing Liang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Synthetic Dyes ◽  
Complex Chemical ◽  
Chemical Structures ◽  
Cultural Conditions ◽  
Cultural Medium ◽  
Initial Ph

Dyes are usually difficult to be decolorized due to their complex chemical structures. In this work, a bacterium which had the ability of decoloration on synthetic dyes was isolated from Yancheng printworks and was identified as Pseudomonas aeruginosa. The effects of concentration of the dye concentration, cultural time, cultural temperature and initial pH of cultural medium on the efficiency of decoloration were investigated. The result showed that the optimal cultural conditions was: dye concentration was 50mg.L-1, cultural time was 72 h, cultural temperature was 28°C, initial pH of cultural medium was 7.0.

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