scholarly journals Antibacterial Activity ofPseudonocardiasp. JB05, a Rare Salty Soil Actinomycete againstStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Nesa Jafari ◽  
Reza Behroozi ◽  
Davoud Farajzadeh ◽  
Mohammad Farsi ◽  
Kambiz Akbari-Noghabi
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Scale Up ◽  
Diffusion Method ◽  
Hydroxyl Groups ◽  
Sephadex Column ◽  
Antibacterial Compounds ◽  
Antibacterial Compound ◽  
Layer Chromatography

Staphylococcus aureusis a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds againstS. aureusand characterize the structure of the resultant antibacterial compounds. Therefore, a slightly modified agar well diffusion method was used to determine the antibacterial activity of actinomycete isolates against the test microorganisms. The bacterial extracts with antibacterial activity were fractionated by silica gel and G-25 sephadex column chromatography. Also, the active fractions were analyzed by thin layer chromatography. Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy. One of the isolates, which had a broad spectrum and high antibacterial activity, was designated asPseudonocardiasp. JB05, based on the results of biochemical and 16S rDNA gene sequence analysis. Minimum inhibitory concentration for this bacterium was 40 AU mL−1againstS. aureus. The antibacterial activity of this bacterium was stable after autoclaving, 10% SDS, boiling, and proteinase K. Thin layer chromatography, using anthrone reagent, showed the presence of carbohydrates in the purified antibacterial compound. Finally, FT-IR spectrum of the active compound illustrated hydroxyl groups, hydrocarbon skeleton, and double bond of polygenic compounds in its structure. To the best of our knowledge, this is the first report describing the efficient antibacterial activity by a local strain ofPseudonocardia.The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward thePseudonocardiascale-up for the production and identification of the antibacterial compounds.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

scholarly journals Microbiological Method for Determining Macrolides in Animal Feeds in the Presence of Other Drugs by Thin-Layer Chromatography Detection

1996 ◽  
Vol 79 (6) ◽  
pp. 1263-1268 ◽  
Author(s):  
Pantelis K Markakis
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Animal Feed ◽  
Diffusion Method ◽  
Growth Promoter ◽  
Animal Feeds ◽  
Relative Standard ◽  
Layer Chromatography ◽  
Method Analysis ◽  
Feed Samples

Abstract A method was developed to separate, detect, and quantitate erythromycin (ERY) and tylosin (TYL) in animal feeds in the presence of 11 other drugs: 3 nitrofurans, 2 tetracycline antibiotics, 3 sulfonamides, 2 coccidiostats, and 1 antibacterial growth promoter. ERY and TYL were separated from coexisting drugs, detected by thin-layer chromatography, and quantitated microbiologically by an agar diffusion method. Analysis of 125 experimental animal feed samples fortified at 5 levels (7.5–400 ppm) with ERY and TYL and at 1 level (50 ppm) with the rest of the drugs gave limits of quantitation of 2 and 5 ppm, recoveries of 90.3 and 92.4%, and relative standard deviations of 4.3–7.3% and 3.6–6.1%, respectively.

scholarly journals KAJIAN FRAKSI METANOL DARI EKSTRAK METILEN DIKLORIDA KULIT KAYU BATANG PELAWAN (Tristania whitiana Griff.) SEBAGAI ANTIBAKTERI

Molekul ◽  
2007 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Purwantiningsih Sugita
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inhibitory Concentration ◽  
Methanol Fraction ◽  
The Third ◽  
The One ◽  
Layer Chromatography ◽  
Chloroform Methanol

Antibacterial compound from methanol fraction of methylene dichloride extract of pelawan bark had been studied. Pelawan bark was extracted using ethanol 95% and CH2Cl2-water (1:1), respectively. Extract of CH2Cl2 was partitioned using hexane-methanol 90% (1:1). Afterwards, methanol fraction was analyzed by thin layer chromatography methods to find the best eluent for column chromatography. The best eluent came from mixture of acetone-chloroform-methanol (0,6:4:0,4). The fractionation yielded 16 fractions with the first (M1) and the third (M5) fractions became the one spotted fractions. M1 and M5 fractions were examined for their antibacterial activity using paper diffusion methods by determining minimum inhibitory concentration value. M1 fraction showed greater activity than M5 fraction with minimum inhibitory concentration value of 2,7857 mg/mL. The result of phytochemistry test showed that both fractions contain terpenoid.

scholarly journals Quantification of Lupeol as Secondary Metabolite by HPTLC Technique and Assessment of Antimicrobial Potential of Ethyl Acetate Extract of Betula alnoides Bark

2021 ◽  
Vol 37 (2) ◽  
pp. 426-432
Author(s):  
Shahbaz Khan ◽  
Harpreet Singh ◽  
Arun K Mishra ◽  
Najam Ali Khan
Keyword(s):  
Antifungal Activity ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Antimicrobial Potential ◽  
Betula Alnoides ◽  
Layer Chromatography

The present work includes extraction of Betula alnoides bark using ethyl acetate as a solvent, preliminary phytochemical test, quantification of phytochemicals and quantification of lupeol in Betula alnoides by High Performance Thin Layer Chromatography (HPTLC) instrument along with the assessment of the antimicrobial potential of Ethyl Acetate Extract (EAE). The marc obtained after defatting of the coarsely powdered crude drug in Petroleum ether (60-80) was extracted using ethyl acetate. Afterward, preliminary phytochemical tests were done. For High Performance Thin Layer Chromatography (HPTLC), the solvent used was n-hexane: ethyl acetate (8:2 v/v) and scanning was performed at wavelength 254 nm. EAE was screened for antimicrobial potential. The extraction yield was 3.45% w/w. The result of the phytochemical analysis confirmed the presence of some important phytochemicals in EAE. A clear and resolved peak of lupeol was observed at Rf 0.61. The developed method was validated as per ICH guidelines. The concentration (%) of the marker compound (lupeol) was found to be 0.0168. Disk diffusion method using Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis as bacterial strains and Candida albicans, Aspergillus flavus and Epidermophyton floccosum as fungal strains against ciprofloxacin (for antibacterial activity) and fluconazole (for antifungal activity) as standard drugs was employed. The finding suggested that EAE possess significant antibacterial and antifungal activity when comparison was made with standard drugs. The proposed elucidated mechanism behind this action may be due to the presence of triterpenoids in EAE.

scholarly journals Determination of Tetracyclines in Animal Feeds in the Presence of Other Drugs by Thin-Layer Chromatography and Microbiological Method

1996 ◽  
Vol 79 (2) ◽  
pp. 375-379 ◽  
Author(s):  
Pantelis K Markakis
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Animal Feed ◽  
Diffusion Method ◽  
Growth Promoter ◽  
Animal Feeds ◽  
Limit Of Quantitation ◽  
Layer Chromatography ◽  
Method Analysis

Abstract This method was developed to separate, detect, and quantitate oxytetracycline (OTC) or chlortetracycline hydrochloride (CTC HCI) in animal feeds in thepresence of 11 otherdrugs: 3 nitrofurans, 2 macrolideantibiotics,3 sulfonamides, 2 coccidiostatics, and 1 antibacterial growth promoter. OTC or CTC HCI was separated from coexisting drugs and detected by thin-layer chromatography, then quantitated microbiologically by the agar diffusion method. Analysis of 125 experimental animal feed samples fortified at 5 levels (7.5-400 ppm) with OTC or CTC HCI and at 1 level (50 ppm) with the rest of the drugs, respectively, gave a limit of quantitation of 1.25 or 0.625 ppm, a recovery of 90.6 or 92.9%, and a coefficient of variation of 2.9-6.1 or 2.3-4.4%.

scholarly journals Extraction and Isolation of Antioxidant-antibacterial Compounds From Lactobacillus casei Strain K1C by Thin-layer Chromatography

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Zahra Pourramezan ◽  
Mana Oloomi ◽  
Rouha Kasra Kermanshahi ◽  
Hassan Rezadoost
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Growth Phase ◽  
Stationary Growth Phase ◽  
Stationary Growth ◽  
Food Industries ◽  
Layer Chromatography ◽  
Minimum Medium ◽  
Mrs Broth

Background: Nowadays, searching for natural bioactive compounds with potential use in food industries is a major issue. Because of simple purification, natural compounds from microbial sources attract more attention. These encompass antioxidant and antibacterial materials derived from probiotics. Methods: In this study, Lactobacillus strains were isolated from kefir specimens. The antioxidant and antibacterial activity of the methanol extract of the supernatants was determined using 2, 2-diphenyl-picyril hydrazil (DPPH) and minimum inhibitory concentration (MIC) methods, respectively. In order to increase the antioxidant properties, a minimum medium fermented aerobically was used. Results: Antibacterial activity of Lactobacillus supernatant increased against E. coli ATCC 11303 in case of minimum medium (25.32 mg/mL) compared to MRS broth (32 mg/mL); however, aerobic condition decreased antibacterial production (65.44 mg/mL). After fractionation by thin-layer chromatography (TLC), this value reached the highest level (500 µg/mL). Production analysis at different times showed that maximum antibacterial activity was obtained in the middle of the logarithmic growth phase until the beginning of the stationary growth phase. The antioxidant traits increased significantly in minimum culture media and anaerobic condition (492.1 ± 0.25 µg/mL) compared to the similar condition in MRS broth (880.96 ± 0.05 µg/mL). The highest antioxidant production was observed in the stationary growth phase of the aerobically fermented minimum medium (266.82 ± 0.17 µg/mL). Conclusions: The findings of this study showed that the best antibacterial and antioxidant-producing isolate, L. casei strain K1C (accession no.: KU954559), could be useful as a natural preservative in food industries.

scholarly journals Studies on the Antibacterial Activity and Chemical Composition of Methanol Extract of Cochlospermum tinctorium Root

2019 ◽  
pp. 1-11
Author(s):  
R. Abdulaziz ◽  
M. H. Usman ◽  
U. B. Ibrahim ◽  
B. M. Tambari ◽  
A. Nafiu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extract ◽  
Methanol Extract ◽  
Antibiotic Resistant ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Layer Chromatography

The aim of study was to evaluate the antibacterial activity of Cochlospermum tinctorium against ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene. Ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene procured from Microbiology Research Laboratory Usman Danfodiyo University Sokoto. The roots of Cochlospermum tinctorium were collected from the rock side in Dambu Gomo, Zuru Local Government Area of Kebbi State, Nigeria. The roots were washed, air-dried and milled to powder using mortal and pestle and sieved to obtained fine powder. Maceration was used for extraction using methanol as solvent. The antibacterial activity of the plant was determined on Mueller Hinton agar using agar well diffusion method. Minimum concentration (MIC) and minimum inhibitory concentration (MBC) of plant extract was also determined. Thin layer chromatography and column chromatography was employed for separation and fraction of different compounds in the plant extract. The fractions were screened for antibacterial activity and active fractions having high antibacterial activity were subjected Gas Chromatography Mass Spectoscopy (GC-MS) analysis. The result of methanol extraction yield 5.17% extracts. The methanol extract of Cochlospermum tinctorium was effective in inhibiting the isolates at high concentration of 10 mg/mL. The results thin layer chromatography revealed four spots with Rf values 0.02, 0.37, 0.44 and 0.80 respectively. The GC-MS analysis of the active methanol extract of Cochlospermum tinctorium root powder revealed the existence of major peaks 1-(+)-Ascorbic acid 2,6-dihexadecanoate (R.T: 13.666), Diethyl phthalate (R.T: 10.440), Undecyl acetate (R.T: 10.007), 3-tetradecanone (R.T: 9.793), 3-hexadecanone (R.T: 12.427). It therefore concluded that the root of Cochlospermum tinctorium has immense potential to be used in the area of pharmacology as it possess antimicrobial activity against the antibiotic resistant food-borne pathogens, thus could be exploited as alternative antimicrobial drugs.

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

scholarly journals Antioxidant Activity of Flavonoid from Rhizome Kaemferia galanga L. Extract

ALCHEMY ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 127
Author(s):  
Elok Kamilah Hayati ◽  
Rachmawati Ningsih ◽  
Latifah Latifah
Keyword(s):  
Antioxidant Activity ◽  
Herbal Medicine ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Hydroxyl Groups ◽  
Chloroform Fraction ◽  
Test Results ◽  
Layer Chromatography

<p><em>Kaemferia galanga</em> L. rhizome is a traditional crop which is widely used for herbal medicine.  This research is started from the extraction of maceration using ethanol 80% and partition with chloroform:water. 80 % ethanol extract and chloroform fraction tested antioxidant activity with DPPH (<em>1,1-diphenyl-2-picrylhydrazyl)</em>, phytochemicals with reagents, separation of the active compound by TLC (<em>thin layer chromatography)</em> analytical and preparative TLC, the identification of compounds with sliding reagent using UV-Vis. The test results on the antioxidant activity of 80% ethanol extract of 64,93% (100 ppm) and chloroform fraction of 54,9 % (100 ppm) with indigo IC50 of 13,07 mg/mL and 81,9 mg mL. 80 % ethanol extract and chloroform fraction has potential as a source of natural antioxidants. Phytochemical test showed 80% ethanol extract contains flavonoids, alkaloids and tannins. At the chloroform fraction extract containing flavonoids. The separation of the active compounds with analytical TLC using eluent PE: ethyl acetate (5: 1) resulted in 7 stain (Rf 0,06 to 0,96) and preparative TLC produce 8 spots (Rf from 0,19 to 0,83). Results from UV-Vis spectrum can be presumed that such a class of flavonoid compounds flavanones or dihidroflavonol. It can be seen from the wavelength range 210-285 nm in the bands I and 300-550 nm in band II. The addition of the reagent slide showed the presence of hydroxyl groups in ring A, B and C and O-hydroxy on ring A.</p><p class="BodyAbstract"> </p><p class="BodyAbstract"><strong>Keywords</strong><strong>:</strong> antioxidant activity, Kaemferia galanga rizhome, sliding reagent, Thin Layer Chromatography, 1,1-diphenyl-2-picrylhydrazyl</p><p class="BodyAbstract"> </p>

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