scholarly journals Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Lili Wen ◽  
Yang Huang ◽  
Xianbiao Xie ◽  
Wan Huang ◽  
Junqiang Yin ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Open Field ◽  
Field Test ◽  
Formalin Test ◽  
Open Field Test ◽  
Interleukin 1 ◽  
Paw Edema ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Anti Inflammatory

The aims of this study were to evaluate the anti-inflammatory and analgesic effects of bufalin, a major component of “Chan-su.” We used a carrageenan-induced paw edema model to assess the anti-inflammatory activity of this compound, and Western blot analysis detected NF-κB signaling during this effect. The antinociceptive activities were evaluated by acetic acid-induced writhing, formalin, and hot-plate tests; open-field test investigated effects on the central nervous system. Our data showed that bufalin (0.3 and 0.6 mg/kg, i.p.) potently decreased carrageenan-induced paw edema. Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β(IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α(TNF-α) during these treatments. Further studies demonstrated that bufalin significantly inhibited the activation of NF-κB signaling. Bufalin also reduced acetic acid-induced writhing and the licking time in the formalin test and increased hot-plate reaction latencies. Naloxone pretreatment (2 mg/kg, i.p.) in the early phases of the formalin test and hot-plate test significantly attenuated the bufalin-induced antinociception effects, which suggests the involvement of the opioid system. A reduction in locomotion was not observed in the open-field test after bufalin administration. Taken together, bufalin treatment resulted in in vivo anti-inflammatory and analgesic effects, and bufalin may be a novel, potential drug for the treatment of inflammatory diseases.

scholarly journals Antinociceptive and Anti-Inflammatory Effects of Bixin, a Carotenoid Extracted from the Seeds of Bixa orellana

Planta Medica ◽  
2019 ◽  
Vol 85 (16) ◽  
pp. 1216-1224 ◽  
Author(s):  
Samanta Daliana Golin Pacheco ◽  
Alexia Thamara Gasparin ◽  
Carlos Henrique Alves Jesus ◽  
Bruna Bittencourt Sotomaior ◽  
Ana Clara Sans Salomão Brunow Ventura ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Antinociceptive Effect ◽  
Pharmacological Properties ◽  
Bixa Orellana ◽  
Paw Edema ◽  
Hot Plate ◽  
Anti Inflammatory

AbstractBixin is the main natural apocarotenoid extracted from the seeds of Bixa orellana, widely used as a cosmetic and textile colorant. Despite the description of several pharmacological properties of B. orellana extracts, little has been studied regarding the pharmacological properties of bixin. Then we aimed to investigate the potential anti-inflammatory and antinociceptive effect of bixin in preclinical models of inflammation and acute pain. The anti-inflammatory activity of bixin (15 or 30 mg/kg, orally) was determined using carrageenan-induced paw edema and the myeloperoxidase (MPO) activity in male Wistar rats. The antinociceptive effect of bixin was assessed in the formalin and hot plate tests in rats (at same doses) and in the acetic acid-induced writhing test in Swiss albino male mice (at doses of 27 or 53 mg/kg). General locomotor activity was evaluated in the open field test. Only the higher dose of bixin significantly decreased the carrageenan-induced paw edema and the MPO activity and increased the latency time in the hot plate. Both doses of bixin significantly reduced the number of flinches in both phases of the formalin test and the number of acetic acid-induced writhings without changing the locomotor performance in the open field test. This study validates the use of bixin as an anti-inflammatory trough mechanism related to the reduction of neutrophil migration. Furthermore, this is the first report showing the antinociceptive property of bixin, which does not appear to be related to the sedative effect. Further studies are necessary to characterize the mechanisms involved in these effects.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

Analgesic and Anti-Inflammatory Activities of the Methanol Extract ofElaeagnus oldhamiiMaxim. in Mice

2012 ◽  
Vol 40 (03) ◽  
pp. 581-597 ◽  
Author(s):  
Chi-Ren Liao ◽  
Yuan-Shiun Chang ◽  
Wen-Huang Peng ◽  
Shang-Chih Lai ◽  
Yu-Ling Ho
Keyword(s):  
Acetic Acid ◽  
Formalin Test ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Late Phase ◽  
Paw Edema ◽  
Hplc Fingerprint ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory

We investigated possible mechanisms of analgesic and anti-inflammatory activities of the methanol extract from the leaf of Elaeagnus oldhamii Maxim. (EOMeOH). EOMeOHwas evaluated for its analgesic activity in acetic acid-induced writhing response and formalin test, and anti-inflammatory effect was examined by λ-carrageenan-induced paw edema assay. We detected the activities of GPx, GRd and SOD in the liver, and the levels of inflammatory mediators including IL-1β, IL-6, TNF-α, COX-2, MDA and NO in the edema paw to investigate the mechanism of action against inflammation. Total polyphenol, flavonoid and flavanol contents of EOMeOHwere detected to explore its antioxidant activities. Results showed that, in the analgesic test, EOMeOHdecreased acetic acid-induced writhing response and the licking time in the late phase of formalin test. In the anti-inflammatory test, EOMeOHdecreased paw edema at the 2nd, 3rd, 4th and 5th h after λ-carrageenan had been injected. EOMeOHincreased the activities of SOD and GPx in liver tissue and decreased MDA, NO, IL-1β, IL-6, TNF-α and COX-2 levels in paw edema tissue at the 3rd h after λ-carrageenan-induced inflammatory reaction. EOMeOHexhibited abundant polyphenol, flavonoid and flavanol contents. In HPLC fingerprint test of EOMeOH, two index ingredients, ursolic acid and pomolic acid, were isolated from EOMeOHand were exhibited in HPLC chromatographic analysis. The results demonstrated analgesic and anti-inflammatory effects of EOMeOH. It was indicated that the anti-inflammatory mechanism of EOMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx and GRd in the liver. Additionally, EOMeOHdecreased IL-1β, IL-6, TNF-α and COX-2 levels in the edema paw. The results suggested its value in future development of herbal medicine for the treatment of inflammatory diseases.

scholarly journals Analgesic and Anti-Inflammatory Activities of Resveratrol through Classic Models in Mice and Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Guangxi Wang ◽  
Zhiqiang Hu ◽  
Xu Song ◽  
Qiankun Cui ◽  
Qiuting Fu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Activities ◽  
Drug Candidate ◽  
Hot Plate ◽  
Sod Activity ◽  
Analgesic Effects ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Ear Oedema

Background. Inflammation and pain are closely related to humans’ and animals’ health.Resveratrol(RSV) is a natural compound with various biological activities. The current study is aimed to evaluate the analgesic and anti-inflammatory activities of RSV in vivo. Materials and Methods. The analgesic effects were assessed by the acetic acid-induced writhing and hot plate tests. The anti-inflammatory effects were determined using the xylene-induced mouse ear oedema, the acetic acid-induced rat pleurisy, and carrageenan-induced rat synovitis tests, respectively.Results. The analgesic results showed that RSV could significantly inhibit the number of writhes and improve the time and pain threshold of mice standing on hot plate. The anti-inflammatory results showed that RSV could inhibit the ear oedema of mice. In acetic acid-induced pleurisy test, RSV could significantly inhibit the WBC and pleurisy exudates, could decrease the production of NO, and elevate the activity of SOD in serum. In carrageenan-induced synovitis test, RSV could reduce the content of MDA and elevate the T-SOD activity in serum; RSV could inhibit the expressions of TP, PGE2, NO, and MDA.Conclusion. Shortly, these results indicated that RSV had potent analgesic and anti-inflammatory activities and could be a potential new drug candidate for the treatment of inflammation and pain.

scholarly journals Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chi-Ren Liao ◽  
Chun-Pin Kao ◽  
Wen-Huang Peng ◽  
Yuan-Shiun Chang ◽  
Shang-Chih Lai ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Paw Edema ◽  
Tissue Destruction ◽  
Hplc Fingerprint ◽  
Analgesic Effects ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Anti Inflammatory

This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism ofFPMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,FPMeOHalso decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment ofFicus pumilain inflammatory diseases.

scholarly journals INVESTIGATION OF ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF AQUEOUS EXTRACTS OF ARTEMISIA AFRA IN WISTAR RATS

2018 ◽  
Vol 11 (12) ◽  
pp. 190 ◽  
Author(s):  
Mavuto Gondwe ◽  
Anda Mpalala ◽  
Lusanda Zongo ◽  
David Kamadyaapa ◽  
Eugene Ndebia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Effects ◽  
Control Group ◽  
Paw Edema ◽  
Hot Plate ◽  
Traditional Practice ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Antinociceptive Effects ◽  
Anti Inflammatory

Objective: The objective of the study was to evaluate the anti-inflammatory and antinociceptive effects of Artemisia afra.Methods: Animals were randomly divided into five groups of six animals each and administered with normal saline (2 ml/kg), indomethacin (10 mg/ kg), and A. afra at doses of 100, 200, and 400 mg/kg, respectively. For the anti-inflammatory activity, carrageenan-induced paw edema was used while the hot plate and acetic acid induced-writhing tests were used to assess the antinociceptive activity.Results: Pretreatment with A. afra at a dose of 100 mg/kg did not show any significant biological effects (p>0.05) for any of the three tests, when compared against saline-treated control group. At a dose of 200 mg/kg, A. afra demonstrated significant effects (p<0.01), during the 5th h reducing carrageenan-induced paw edema by 12%. The highest dose (400 mg/kg) of A. afra demonstrated more potent effects by decreasing the carrageenan-induced paw swelling (p<0.001–0.05) during the 3rd, 4th, and 5th h, by up to 38% when compared against saline-treated control group. Both the 200 and 400 mg/kg, A. afra doses achieved a significant increase (p<0.05) in reaction time in the hot plate test. In the acetic acid-induced writhing test, pretreatment with A. afra (400 mg/kg) significantly reduced pain by 39% (p<0.01) by comparison with the saline control.Conclusion: Experimental data demonstrated that aqueous extract of A. afra possesses anti-inflammatory and antinociceptive properties in experimental acute inflammation and pain. These findings support the usage of A. afra in managing inflammation and pain in traditional practice.

EVALUATION OF ANALGESIC AND ANTIINFLAMMATORY ACTIVITY OF CUSCUTA REFLEXA EXTRACTS ON ANIMAL MODELS

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (10) ◽  
pp. 54-62
Author(s):  
P. B Udavant ◽  
◽  
V.S Suggala ◽  
S. J. Kshirsagar ◽  
C. D. Upasani
Keyword(s):  
Acetic Acid ◽  
Formalin Test ◽  
Methanol Extract ◽  
Biological Activities ◽  
Second Phase ◽  
Thermal Stimulus ◽  
Paw Edema ◽  
Antiinflammatory Effect ◽  
Cuscuta Reflexa ◽  
Anti Inflammatory

Cuscuta reflexa (Roxb.) belonging to the family Convolvulaceae and popularly known as dodder, is a parasitic plant with claims of antibacterial, antiproliferative and anti-inflammatory properties in literature. HPTLC analysis and investigation of analgesic and anti-inflammatory activities of Cuscuta reflexa extracts, petroleum ether extract, methanol extract, and aqueous extract of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 & 300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acid-induced writhing response and formalin test. The anti-inflammatory activity was determined using models like carrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test and leukocyte migration test using a single dose of 300 mg/kg orally. Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/ kg p.o.) significantly (P< 0.05) increased latency against thermal stimulus, decreased the acetic acidinduced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema. The most promising fraction of methanol extract was found to be quercetin, which could be responsible for the biological activity. Results suggest significant analgesic and antiinflammatory effects produced by MECR and AECR.

Antinociceptive and anti-inflammatory properties of Tetracera alnifolia Willd. (Dilleniaceae) hydroethanolic leaf extract

2018 ◽  
Vol 30 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Olufunmilayo O. Adeyemi ◽  
Ismail O. Ishola ◽  
Elizabeth T. Adesanya ◽  
Destiny O. Alohan
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Potassium Channel ◽  
Leaf Extract ◽  
Paw Edema ◽  
Hot Plate ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Tetracera Alnifolia ◽  
Inflammatory Effect

Abstract Background Tetracera alnifolia Willd. (Dilleniaceae) is used in traditional African Medicine for the treatment of headache, abdominal pain, and rheumatism. Hence, this study sought to investigate the antinociceptive and anti-inflammatory effects of the hydroethanolic leaf extract of T. alnifolia (HeTA) in rodents. Methods Antinociceptive activity was evaluated using the acetic acid-induced writhing, formalin-/capsaicin-induced paw licking and hot plate tests in mice. The contribution of opioidergic, l-arginine-nitric oxide, and ATP-sensitive potassium channel pathways in HeTA-induced antinociception was also evaluated. The anti-inflammatory effect was assessed using the carrageenan-induced paw edema, xylene ear edema, cotton pellet granuloma, and complete Freund’s adjuvant (CFA)-induced arthritis in rats. Results HeTA (100, 200, and 400 mg/kg, p.o.) produced significant (p<0.05) decrease in mean number of acetic acid-induced writhing, time spent licking paw in formalin, and capsaicin tests as well as time course increase in nociceptive reaction latency in hot plate test. HeTA-induced antinociception was prevented by pretreatment of mice with naloxone (non-selective opioid receptor antagonist), l-arginine (nitric oxide precursor), or glibenclamide (ATP-sensitive potassium channel blocker). HeTA (100 mg/kg, p.o.) produced a significant anti-inflammatory effect against carrageenan-induced rat paw edema (1–5 h), xylene-induced ear edema, cotton pellet-induced granuloma formation, and CFA-induced arthritis in rats. The effects of HeTA in various models were similar to the effect of the standard reference drugs. Conclusions Findings from this study showed that HeTA possesses antinociceptive effect possibly mediated through peripheral opioid receptors with activation of l-arginine-nitric oxide and ATP-sensitive potassium channel pathway as well as anti-inflammatory activity.

scholarly journals Analgesic and Antiinflammatory Activities of the Essential Oil of the Unique Plant Zhumeria majdae

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Seyyedeh Ghazal Miraghazadeh ◽  
Hamed Shafaroodi ◽  
Jinous Asgarpanah
Keyword(s):  
Acetic Acid ◽  
Essential Oil ◽  
Diclofenac Sodium ◽  
Biologically Active ◽  
Significant Activity ◽  
Paw Edema ◽  
Active Constituent ◽  
Hot Plate ◽  
Standard Drug ◽  
Anti Inflammatory

The analgesic and anti-inflammatory properties of Zhumeria majdae essential oil were investigated in mice and rats. The analgesic activity of the oil was assessed by acetic acid-induced writhing and Eddy's hot plate methods while the acute anti-inflammatory effect was investigated by inflammatory paw edema in rat. Z. majdae oil significantly decreased the number of acetic acid-induced writhes in mice compared with animals that received vehicle only. Also it exhibited a central analgesic effect as evidenced by a significant increase in reaction time in the hot plate method. The oil also significantly reduced carrageenan induced paw edema in rats. The inhibitory activity of Z. majdae essential oil was found to be very close to that of the standard drug, diclofenac sodium (50 mg/kg). The studied oil was analyzed by GC and GC–MS and seventeen constituents were identified, representing 99.2% of the oil. The major components of the oil were characterized as linalool (63.4%) and camphor (27.5%), which might be responsible for these observed activities. The results suggest that Z. majdae essential oil possesses biologically active constituent(s) that have significant activity against acute inflammation and have central and peripheral antinociceptive effects which support the ethnomedicinal claims of the plant application in the management of pain and inflammation.

scholarly journals Ameliorative Effects of Scopoletin fromCrossostephium chinensisagainst Inflammation Pain and Its Mechanisms in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Tien-Ning Chang ◽  
Jeng-Shyan Deng ◽  
Yi-Chih Chang ◽  
Chao-Ying Lee ◽  
Liao Jung-Chun ◽  
...  
Keyword(s):  
Tumor Necrosis ◽  
Formalin Test ◽  
Late Phase ◽  
No Synthase ◽  
Paw Edema ◽  
Analgesic Effects ◽  
Anti Oxidant ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Necrosis Factor

Scopoletin exists in nature as an anti-oxidant, hepatoprotective, and anti-inflammatory activities reagent. In this study, we have investigated the analgesic effects of the scopoletin using the models of acetic acid-induced writhing response and the formalin test, the anti-inflammatory effects of scopoletin using model ofλ-carrageenan (Carr)-induced paw edema. The treatment of ICR mice with scopoletin inhibited the numbers of writhing response and the formalin-induced pain in the late phase. This study demonstrated that the administration of scopoletin resulted in the reduction of Carr-induced mice edema, and it increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) after Carr injection. We also demonstrated scopoletin significantly attenuated the malondialdehyde (MDA) level in the edema paw after Carr injection. Scopoletin decreased the NO, tumor necrosis factor (TNF-α) and prostaglandin E2 (PGE2) levels on serum after Carr injection. Scopoletin decreased Carr-induced inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions in the edema paw. These anti-inflammatory mechanisms of scopoletin might be related to the decrease in the level of MDAviaincreasing the activities of SOD, CAT, and GPx in the edema paw. Also, scopoletin could affect the production of NO, TNF-α, and PGE2, and therefore affect the anti-inflammatory effects.

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