scholarly journals Fungal Exposure and Low Levels of IL-10 in Patients with Sarcoidosis

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Marjeta Terčelj ◽  
Sanja Stopinšek ◽  
Alojz Ihan ◽  
Barbara Salobir ◽  
Saša Simčič ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Serum Levels ◽  
Healthy Controls ◽  
Serum Cytokine ◽  
Fungal Cell ◽  
Fungal Exposure ◽  
Cytokine Levels ◽  
Exposure Levels ◽  
Low Levels ◽  
Anti Inflammatory Cytokine

Background and Objectives. Sarcoidosis is an inflammatory disease with increased levels of inflammatory cytokines. Previous studies have shown a relation between the degree of granuloma infiltration and serum cytokine levels, except for interleukin- (IL-) 10. The aim of the study was to further investigate the serum levels of IL-10 in patients with sarcoidosis and relate them to fungal exposure in terms of the amount of fungi in the air of their homes andβ-glucan in bronchoalveolar lavage (BAL) fluid.Methods. Patients with sarcoidosis (n=71) and healthy controls (n=27) were enrolled. IL-10 was determined in serum. BAL was performed and the amount ofβ-glucan was measured. Domestic exposure to fungi was determined by measuring airborneβ-N-acetylhexosaminidase (NAHA) in the bedrooms.Results. At high levels of fungal exposure (domestic fungal exposure andβ-glucan in BAL), serum IL-10 values were lower than at low and intermediate exposure levels.Conclusion. The low serum IL-10 values at high fungal exposure suggest that fungal cell wall agents play a role in granuloma formation in sarcoidosis by inhibiting the secretion of the anti-inflammatory cytokine IL-10.

scholarly journals Cytokine profiles in highly active antiretroviral treatment non-adherent, adherent and naive HIV-1 infected patients in Western Kenya

2021 ◽  
Vol 21 (4) ◽  
pp. 1584-92
Author(s):  
Fauzia Musa ◽  
Nathan Shaviya ◽  
Fidelis Mambo ◽  
Collins Abonyo ◽  
Erick Barasa ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Antiretroviral Treatment ◽  
Healthy Controls ◽  
Highly Active ◽  
Cytokine Balance ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine ◽  
Hiv 1

Background: Cytokines play an important role in signaling the immune system to build an adequate immune responseagainst HIV. HIV distorts the balance between pro and anti-inflammatory cytokines causing viral replication. Highly active antiretroviral treatment (HAART) acts by trying to restore pro and anti-inflammatory cytokine balance. It is not clear how HAART non-adherence influences circulating cytokine levels. This study therefore determined cytokine levels in HAART non-adherent individuals. Methods: This cross-sectional study recruited 163 participants (51 controls, 23 HIV-1+ HAART naive, 28 HAART-adherent6 months, 19 HAART-adherent 12 months and 42 HAART non-adherent). Cytokines were analyzed by ELISA while CD4 T cells determined in 3.0 μl of whole blood using BD FACSCaliburTM and viral load in 0.2ml plasma sample using Abbott Molecular m2000sp sample preparation and m2000rt real-time amplification and detection systems (Abbott MolecularInc., Illinois, USA) according to the manufacturer’s methods. Results: IL-4, IL-6, IL-10, TNF-α and TGF-β were significantly elevated in HIV-1 HAART non-adherent compared withHIV-1 HAART adherent and healthy controls P<0.01. IFN- γ was significantly decreased in HIV-1 HAART non-adherentcompared with HIV-1 HAART adherent and healthy controls P<0.01. TNF-α and TGF-β were significantly reduced in HIV-1 HAART adherent patients at 12 months compared to those at 6 months P<0.01. IL-4 and IL-10 correlated positively withviral load. IL-4, IL-6, IL-10, TNF-α and TGF- β associated inversely with CD4 T cell counts and body mass index (BMI). Conclusion: This study established that HAART adherence is immunologically beneficial to the pro and anti-inflammatory cytokine balance milieu while non-adherence appears to cause alterations in pro and anti-inflammatory cytokines warping the balance in this dichotomy. Keywords: Cytokines; non-adherence; HAART.

scholarly journals Neurotrophins, cytokines, oxidative parameters and funcionality in Progressive Muscular Dystrophies

2015 ◽  
Vol 87 (3) ◽  
pp. 1809-1818 ◽  
Author(s):  
CLARISSA M. COMIM ◽  
GISIANE B. MATHIA ◽  
ANDREZA HOEPERS ◽  
LISIANE TUON ◽  
FLÁVIO KAPCZINSKI ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Muscular Dystrophy ◽  
Inflammatory Cytokine ◽  
Serum Levels ◽  
Superoxide Dismutase Activity ◽  
Age And Gender ◽  
Tnf Α ◽  
Cytokine Levels ◽  
And Gender ◽  
Anti Inflammatory Cytokine

We investigated the levels of brain derived-neurotrophic factor (BDNF), cytokines and oxidative parameters in serum and tried to correlate them with the age and functionality of patients with Progressive Muscle Dystrophies (PMD). The patients were separated into six groups (case and controls pared by age and gender), as follows: Duchenne Muscular Dystrophy (DMD); Steinert Myotonic Dystrophy (SMD); and Limb-girdle Muscular Dystrophy type-2A (LGMD2A). DMD patients (±17.9 years old) had a decrease of functionality, an increase in the IL-1β and TNF-α levels and a decrease of IL-10 levels and superoxide dismutase activity in serum. SMD patients (±25.8 years old) had a decrease of BDNF and IL-10 levels and superoxide dismutase activity and an increase of IL-1β levels in serum. LGMD2A patients (±27.7 years old) had an decrease only in serum levels of IL-10. This research showed the first evidence of BDNF involvement in the SMD patients and a possible unbalance between pro-inflammatory and anti-inflammatory cytokine levels, along with decreased superoxide dismutase activity in serum of DMD and SMD patients.

Serum Heat Shock Protein Levels and Cytokine Profiles Are Altered In AML Patients Before Treatment and Following Disease Stabilizing Therapy,

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 3599-3599
Author(s):  
Hanne Fredly ◽  
Håkon Reikvam ◽  
Bjorn T. Gjertsen ◽  
Øystein Bruserud
Keyword(s):  
Valproic Acid ◽  
Heat Shock ◽  
Close Association ◽  
Serum Levels ◽  
Healthy Controls ◽  
Serum Cytokine ◽  
Cytokine Profiles ◽  
Cytokine Levels ◽  
Shock Proteins ◽  
Low Dose Cytarabine

Abstract Abstract 3599 Heat shock proteins (HSPs) maintain cellular homeostasis and function as molecular chaperones assisting protein folding and translocation. Their intracellular levels are up-regulated in response to a wide variety of insults allowing the cell to survive otherwise lethal conditions. Heat shock proteins are also released to the extracellular space and lead to a more efficient cross-presentation to specific T cells and may also initiate release of proinflammatory cytokines, stimulate NK cells and facilitate DC maturation. Extracellular Heat shock protein (HSP) 70 and HSP90 are characteristics of immunogenic apoptosis, and primary human acute myeloid leukemia (AML) cells release these mediators during spontaneous in vitro apoptosis. Primary AML cells also show constitutive release of several cytokines; these heterogeneous mediators interact through local cytokine networks that regulate growth and differentiation of normal as well as malignant hematopoietic cells. In the present study we investigated serum levels of HSP70/HSP90 and the serum cytokine profiles of patients with untreated AML and patients receiving disease-stabilizing treatment based on all-trans retinoic acid (ATRA) plus valproic acid. Serum samples from 82 untreated AML patients, including 42 patients receiving AML-stabilizing therapy, and 20 healthy controls were analysed. HSP and cytokine levels were determined by ELISA and multiplex analyses. Patients with untreated AML showed significantly increased HSP90 levels compared to healthy controls (Mann Whitney U-test, p<0.0001). The serum levels of HSP70 were significantly lower than the HSP90 levels both for the patients and for the healthy controls (p<0.0001), but the HSP70 serum levels did not differ significantly between patients and controls (Fig. 1A). HSP70 and HSP90 serum levels were significantly correlated for the AML patients (Fig. 1B, Spearman, p<0.01, R=0.425). HSP70 levels were not altered during AML-stabilizing palliative treatment, whereas HSP90 levels decreased during treatment with ATRA, valproic acid and low-dose cytarabine. High age and low differentiation of AML cells according to the FAB classification predicted lower HSP90 levels.Fig. 1Fig. 1. AML patients showed an altered serum cytokine profile compared to healthy controls with significantly altered levels of several chemokines, interleukins, growth factors and immunomodulatory cytokines (Tab.1). These cytokine levels were usually increased compared to the healthy controls. Hierarchical cluster analysis of the untreated patients showed a close association between HSP70, HSP90 and Hepatocyte growth factor (HGF) levels, these mediators also showed a significant correlation (Spearman, p<0.01). Furthermore, disease-stabilizing therapy altered the serum cytokine profile, but the profiles differed when ATRA + valproic acid was combined with either theophyllin or low-dose cytarabine. The close association between HSP70, HSP90 and HGF levels was then maintained only in the cytarabine group.Tab.1.CytokinePatientsControlsP-valueIMMUNOMODULATORY CYTOKINES    TNF-α↑8.4 (<1.5–68.5)4.5 (2.3–13.4)0.016INTERLEUKINS    IL-1α↓0.5 (<0.4–7.0)2.0 (2.0–2.9)<0.0001    IL-4↓<4.5 (<4.5–53.9)11.8 (5–17.5)<0.0001    IL-5↑<0.7 (<0.7–39.1)0.9 (<0.7–0.9)0.0001    IL-6↑5.8 (<1.1–204.6)<1.1 (<1.1–7.7)<0.0001    IL-10↑1.3 (<0.3–238.0)0.3 (<0.3–0.8)0.0005CHEMOKINES    CXCL5↓128.4 (<4.1–3252)1283.5 (341–5307)<0.0001    CXCL8/IL-8↑30.5 (<2.0–1103)10.7 (2.2–22.8)0.0003    CXCL10↑56.7 (0.5–1958)15.25 (8.5–58.4)<0.0001GROWTH FACTORS    HGF↑797.3 (1–9703)262.4 (117.3–729.3)<0.0001    GM-CSF↑0.5 (<2.0–17.2)0.9 (0.9–3.6)0.0096 We conclude that both HSP levels and serum cytokine profiles are altered in untreated AML, and the modulation of these serum levels by AML-stabilizing chemotherapy may reflect a possible mechanism for the clinical effect of this treatment strategy. The table shows serum levels for 82 AML patients and 20 healthy controls where significant differences were found (Mann-Whitney U-test, p<0.02). The results are presented as the cytokine analyzed, the alteration in the patients (↑ increased, ↓ decreased), levels in patients and healthy controls (median and range). All cytokine concentrations are presented as pg/ml, for cytokine levels not detected the values are set to < the detection limit. Disclosures: No relevant conflicts of interest to declare.

Analysis of pro‐ and anti‐inflammatory cytokine gene variants and serum cytokine levels as prognostic markers in breast cancer

2018 ◽  
Vol 233 (12) ◽  
pp. 9716-9723 ◽  
Author(s):  
Raman Preet Kaur ◽  
Kanika Vasudeva ◽  
Heena Singla ◽  
Raja Paramjeet Singh Benipal ◽  
Preeti Khetarpal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Inflammatory Cytokine ◽  
Prognostic Markers ◽  
Gene Variants ◽  
Cytokine Gene ◽  
Serum Cytokine ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine

scholarly journals Reactive arthritis and undifferentiated peripheral spondyloarthritis share human leucocyte antigen B27 subtypes and serum and synovial fluid cytokine profiles

Rheumatology ◽  
2020 ◽  
Author(s):  
Jyoti Ranjan Parida ◽  
Sandeep Kumar ◽  
Sakir Ahmed ◽  
Smriti Chaurasia ◽  
Ratnadeep Mukherjee ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Human Leucocyte Antigen ◽  
Healthy Controls ◽  
Hla B27 ◽  
Serum Cytokine ◽  
Cytokine Profiles ◽  
Cytokine Levels ◽  
Peripheral Spondyloarthritis ◽  
Multiplex Cytokine ◽  
Age And Sex

Abstract Objectives Peripheral SpA (pSpA) is comprised of ReA, PsA, enteritis-associated arthritis and undifferentiated pSpA (upSpA). ReA and upSpA share T cell oligotypes and metabolomics in serum and SF. We investigated HLA-B27 subtypes and cytokines in serum and SF that were compared between ReA and upSpA. Methods ReA and upSpA were compared in two cohorts. In cohort I (44 ReA and 56 upSpA), HLA-B27 subtyping was carried out. In cohort II (17 ReA and 21 upSpA), serum and SF cytokines were compared using a multiplex cytokine bead assay (27 cytokines). A total of 28 healthy controls with similar age and sex to cohort II were included for comparison of serum cytokine levels. Results In cohort I, HLA-B27 was positive in 81.8% (36/44) of ReA and 85.71% (48/56) of upSpA patients. HLA-B27 typing was successful in 70 patients (30 ReA and 40 uSpA). HLA-B*2705 was the most common, followed by HLA-B*2704 and HLA-B*2707. Frequencies were the same between ReA and upSpA. In cohort II, 14 cytokines were detectable in the serum of patients. The levels of eight cytokines were higher than in the controls. The cytokine levels of ReA and upSpA were similar. Sixteen cytokines were detectable in the SF of patients. There was no statistical difference in the levels between ReA and upSpA. The cytokine profiles in sera and SF were also similar among HLA-B27-positive and negative patients. Conclusion ReA and upSpA have similar HLA-B27 subtype associations and similar cytokine profiles. They should be considered as a single entity during studies as well as clinical management.

Why does anti-inflammatory cytokine levels increase during memory acquisition?

Neuroscience ◽  
2021 ◽  
Author(s):  
Sergei G. Levin ◽  
Ekaterina V. Pershina ◽  
Nickolay A. Bugaev-Makarovskiy ◽  
Irina Yu. Chernomorets ◽  
Maxim V. Konakov ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Cytokine Levels ◽  
Memory Acquisition ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine

Truncal vagotomy temporarily decreases the pro- and anti-inflammatory cytokine levels in the small intestine

Surgery Today ◽  
2013 ◽  
Vol 44 (6) ◽  
pp. 1123-1127 ◽  
Author(s):  
Takashi Mitsui ◽  
Kazuhiko Fukatsu ◽  
Masashi Yanagawa ◽  
Syunsuke Amenomori ◽  
Etsushi Ogawa ◽  
...  
Keyword(s):  
Small Intestine ◽  
Inflammatory Cytokine ◽  
Truncal Vagotomy ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine

scholarly journals Immunological disturbance in West and Lennox-Gastaut syndromes

1984 ◽  
Vol 42 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Terezinha C. B. Montelli ◽  
Norma G. S. Mota ◽  
M. Terezinha S. Peraçoli ◽  
Elza A. Torres ◽  
M. Thereza Rezkallah-Iwasso
Keyword(s):  
Serum Levels ◽  
Peripheral Blood Lymphocytes ◽  
West Syndrome ◽  
Healthy Controls ◽  
Migration Inhibition ◽  
Leucocyte Migration ◽  
Low Levels ◽  
Leucocyte Migration Inhibition Test ◽  
Leucocyte Migration Inhibition

Cell-mediated and humoral immunity were investigated in 18 patients with West syndrome, 12 with Lennox-Gastaut syndrome and 19 healthy controls. The study included determination of Ô and  peripheral blood lymphocytes, serum levels of IgG, IgA and IgM, skin sensibilization with DNCB, intracutaneous PHA, leucocyte migration inhibition test and lymphocyte blastic transformation in the presence of PHA. Cell-mediated deficiency was detected in 28 children whereas low levels of immunoglobulins were observed only in 6 children. Immunological disturbances were more prominent in children with West syndrome.

scholarly journals Analysis of Serum Cytokines and Single-Nucleotide Polymorphisms of SOD1, SOD2, and CAT in Erysipelas Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Charles C. Emene ◽  
Irina E. Kravchenko ◽  
Gulnaz I. Aibatova ◽  
Albert A. Rizvanov
Keyword(s):  
Body Part ◽  
Serum Levels ◽  
Lower Limbs ◽  
Nucleotide Polymorphisms ◽  
Serum Cytokine ◽  
Single Nucleotide ◽  
Radical Production ◽  
Cytokine Levels ◽  
Group A ◽  
Male Patients

Increased free radical production had been documented in group A (β-hemolytic) streptococcus infection cases. Comparing 71 erysipelas patients to 55 age-matched healthy individuals, we sought for CAT, SOD1, and SOD2 single polymorphism mutation (SNPs) interactions with erysipelas’ predisposition and serum cytokine levels in the acute and recovery phases of erysipelas infection. Whereas female patients had a higher predisposition to erysipelas, male patients were prone to having a facial localization of the infection. The presence of SOD1 G7958, SOD2 T2734, and CAT C262 alleles was linked to erysipelas’ predisposition. T and C alleles of SOD2 T2734C individually were linked to patients with bullous and erythematous erysipelas, respectively. G and A alleles of SOD1 G7958A individually were associated with lower limbs and higher body part localizations of the infection, respectively. Serum levels of IL-1β, CCL11, IL-2Rα, CXCL9, TRAIL, PDGF-BB, and CCL4 were associated with symptoms accompanying the infection, while IL-6, IL-9, IL-10, IL-13, IL-15, IL-17, G-CSF, and VEGF were associated with predisposition and recurrence of erysipelas. While variations of IL-1β, IL-7, IL-8, IL-17, CCL5, and HGF were associated with the SOD2 T2734C SNP, variations of PDFG-BB and CCL2 were associated with the CAT C262T SNP.

Sign in / Sign up

Export Citation Format

Share Document