scholarly journals Detection of Melanoma Metastases in Resected Human Lymph Nodes by Noninvasive Multispectral Photoacoustic Imaging

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Gerrit Cornelis Langhout ◽  
Diederik Johannes Grootendorst ◽  
Omgo Edo Nieweg ◽  
Michel Wilhelmus Jacobus Maria Wouters ◽  
Jos Alexander van der Hage ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Sentinel Node ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Frozen Section ◽  
Photoacoustic Signal ◽  
Melanoma Metastasis ◽  
Prognostic Information ◽  
Melanoma Metastases

Objective. Sentinel node biopsy in patients with cutaneous melanoma improves staging, provides prognostic information, and leads to an increased survival in node-positive patients. However, frozen section analysis of the sentinel node is not reliable and definitive histopathology evaluation requires days, preventing intraoperative decision-making and immediate therapy. Photoacoustic imaging can evaluate intact lymph nodes, but specificity can be hampered by other absorbers such as hemoglobin. Near infrared multispectral photoacoustic imaging is a new approach that has the potential to selectively detect melanin. The purpose of the present study is to examine the potential of multispectral photoacoustic imaging to identify melanoma metastasis in human lymph nodes.Methods. Three metastatic and nine benign lymph nodes from eight melanoma patients were scannedex vivousing a Vevo LAZR©multispectral photoacoustic imager and were spectrally analyzed per pixel. The results were compared to histopathology as gold standard.Results. The nodal volume could be scanned within 20 minutes. An unmixing procedure was proposed to identify melanoma metastases with multispectral photoacoustic imaging. Ultrasound overlay enabled anatomical correlation. The penetration depth of the photoacoustic signal was up to 2 cm.Conclusion. Multispectral three-dimensional photoacoustic imaging allowed for selective identification of melanoma metastases in human lymph nodes.

scholarly journals Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Chuangjia Huang ◽  
Xiaoling Guan ◽  
Hui Lin ◽  
Lu Liang ◽  
Yingling Miao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Good Biocompatibility ◽  
Targeting Ability ◽  
Body Clearance

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

scholarly journals Fabrication of Coaxial and Confocal Transducer Based on Sol-Gel Composite Material for Optical Resolution Photoacoustic Microscopy

Diagnostics ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Masayuki Tanabe ◽  
Tai Chieh Wu ◽  
Makiko Kobayashi ◽  
Che Hua Yang
Keyword(s):  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Sol Gel ◽  
Optical Resolution ◽  
Maximum Amplitude ◽  
Photoacoustic Signal ◽  
Center Frequency ◽  
Photoacoustic Microscopy ◽  
3 Dimensional ◽  
Pulse Echo

We have newly developed coaxial and confocal optical-resolution photoacoustic microscopy based on sol-gel composite materials. This transducer contains a concave-shaped piezoelectric layer with a focus depth of 5 mm and a hole with a diameter of 3 mm at the center to pass a laser beam into a phantom. Therefore, this system can directly detect an excited photoacoustic signal without prisms or acoustic lenses. We demonstrate the capability of the system through pulse-echo and photoacoustic imaging experiments. The center frequency of the fabricated transducer is approximately 7 MHz, and its relative bandwidth is 86%. An ex-vivo experiment is conducted, and photoacoustic signals are clearly obtained. As a result, 2- and 3-dimensional maximum amplitude projection images are reconstructed.

In Vivo and Ex Vivo Sentinel Node Mapping Does Not Identify the Same Lymph Nodes in Colon Cancer

2016 ◽  
Vol 223 (4) ◽  
pp. S32-S33
Author(s):  
Helene S. Andersen ◽  
Astrid LB. Bennedsen ◽  
Stefan K. Burgdorf ◽  
Jens R. Eriksen ◽  
Susanne Eiholm ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Nodes ◽  
Sentinel Node ◽  
Ex Vivo ◽  
Sentinel Node Mapping

scholarly journals CONTRAST-ENHANCED PHOTOACOUSTIC IMAGING USING INDOCYANINE GREEN-CONTAINING NANOPARTICLES

2014 ◽  
Vol 07 (01) ◽  
pp. 1350029 ◽  
Author(s):  
JUNPING ZHONG ◽  
SIHUA YANG
Keyword(s):  
Indocyanine Green ◽  
Contrast Agents ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Signal Amplitude ◽  
Photoacoustic Signal ◽  
Infrared Wavelength ◽  
Contrast Enhanced ◽  
Human Use ◽  
Good Contrast

Contrast agents are attracting a great deal of attention in photoacoustic imaging. Here we introduce an exogenous contrast agent that provides high photoacoustic signal amplitude at the near-infrared wavelength. Our agents consist of Indocyanine green (ICG) and phospholipid–polyethylene glycol (PL–PEG), entitled ICG–PL–PEG nanoparticles. These nanoparticles have overcome numerous limitations of ICG, such as poor aqueous stability, concentration-dependent aggregation and lack of target specificity. ICG–PL–PEG nanoparticles are biocompatible and relatively nontoxic. All the components of ICG–PL–PEG nanoparticles have been approved for human use. Upon pulsed laser irradiation, the nanoparticles are more efficient in producing photoacoustic waves than ICG alone. The results showed that ICG–PL–PEG nanoparticles act as good contrast agents for photoacoustic imaging. These unique ICG–PL–PEG nanoparticles have great potential in clinical applications.

scholarly journals Melanin-dot–mediated delivery of metallacycle for NIR-II/photoacoustic dual-modal imaging-guided chemo-photothermal synergistic therapy

2019 ◽  
Vol 116 (34) ◽  
pp. 16729-16735 ◽  
Author(s):  
Yue Sun ◽  
Feng Ding ◽  
Zhao Chen ◽  
Ruiping Zhang ◽  
Chonglu Li ◽  
...  
Keyword(s):  
Near Infrared ◽  
High Efficiency ◽  
Photoacoustic Imaging ◽  
Photoacoustic Signal ◽  
Single Treatment ◽  
Good Solubility ◽  
Potential Applications ◽  
Good Signal ◽  
Infrared Channel

Discrete Pt(II) metallacycles have potential applications in biomedicine. Herein, we engineered a dual-modal imaging and chemo-photothermal therapeutic nano-agent 1 that incorporates discrete Pt(II) metallacycle 2 and fluorescent dye 3 (emission wavelength in the second near-infrared channel [NIR-II]) into multifunctional melanin dots with photoacoustic signal and photothermal features. Nano-agent 1 has a good solubility, biocompatibility, and stability in vivo. Both photoacoustic imaging and NIR-II imaging in vivo confirmed that 1 can effectively accumulate at tumor sites with good signal-to-background ratio and favorable distribution. Guided by precise dual-modal imaging, nano-agent 1 exhibits a superior antitumor performance and less severe side effects compared with a single treatment because of the high efficiency of the chemo-photothermal synergistic therapy. This study shows that nano-agent 1 provides a promising multifunctional theranostic platform for potential applications in biomedicine.

In vivo and ex vivo sentinel node mapping does not identify the same lymph nodes in colon cancer

2017 ◽  
Vol 32 (7) ◽  
pp. 983-990 ◽  
Author(s):  
Helene Schou Andersen ◽  
Astrid Louise Bjørn Bennedsen ◽  
Stefan Kobbelgaard Burgdorf ◽  
Jens Ravn Eriksen ◽  
Susanne Eiholm ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Nodes ◽  
Sentinel Node ◽  
Ex Vivo ◽  
Sentinel Node Mapping

Sentinel lymph node in endometrial cancer

2007 ◽  
Vol 17 (5) ◽  
pp. 1113-1117 ◽  
Author(s):  
L.A.F Lopes ◽  
S. M Nicolau ◽  
F. F Baracat ◽  
E. C Baracat ◽  
W. J Gonçalves ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Lymph Nodes ◽  
Sentinel Node ◽  
Endometrial Carcinoma ◽  
Frozen Section ◽  
False Negative ◽  
Pelvic Lymphadenectomy ◽  
Clinical Staging ◽  
Blue Dye

The aim of this study was to evaluate the possibility of identifying the sentinel lymph node and involvement of neoplastic cells in patients with endometrial carcinoma limited to the uterus, and also its correlation with the conditions of other pelvic and para-aortic lymph nodes. Forty patients with endometrial carcinoma, clinical staging I and II, were submitted to complete surgical staging through laparotomy, as recommended by FIGO in 1988. The sentinel node was investigated using patent blue dye in the myometrial subserosa. The sentinel node was excised and submitted to frozen section examination of specimen, stained with hematoxylin and eosin (H&E). Afterward, selective bilateral para-aortic and pelvic lymphadenectomy, total hysterectomy with bilateral salpingo-oophorectomy were performed. The lymph nodes excised were examined by means of paraffin-embedded slices stained with H&E and of imunohistochemistry with antikeratin antibody AE1/AE3. The sentinel lymph node was identified in 77.5% of patients (31/40), and 16.1% (5/31) presented neoplastic involvement in the node. In 25 cases of negative sentinel node, 96% (24/25) had no neoplastic involvement, and 4% (1/25) had other lymph node affected (false negative). In nine cases with no sentinel node identified, 55.5% (5/9) had lymph node involvement. The results of this study allow us to conclude that it is possible to identify the sentinel node using the methods described, and the pathologic examination significantly represents the same conditions of other pelvic and para-aortic lymph nodes.

Detection of Melanoma Metastases in Regional Lymph Nodes Using Multispectral Photoacoustic Imaging

2017 ◽  
Vol 225 (4) ◽  
pp. S189 ◽  
Author(s):  
Madalyn Neuwirth ◽  
Andrew J. Sinnamon ◽  
Susan Schultz ◽  
Chandra Seghal ◽  
George Xu ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Photoacoustic Imaging ◽  
Regional Lymph Nodes ◽  
Melanoma Metastases

First experiences of photoacoustic imaging for detection of melanoma metastases in resected human lymph nodes

2012 ◽  
Vol 44 (7) ◽  
pp. 541-549 ◽  
Author(s):  
D.J. Grootendorst ◽  
J. Jose ◽  
M.W. Wouters ◽  
H. van Boven ◽  
J. Van der Hage ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Photoacoustic Imaging ◽  
Melanoma Metastases

scholarly journals The importance of pre-operative axillary ultrasound and intra-operative sentinel lymph node frozen section analysis in patients with early breast cancer – a 3-year study

2011 ◽  
Vol 93 (2) ◽  
pp. 103-105 ◽  
Author(s):  
Melissa M Sanders ◽  
Shamaela Waheed ◽  
Sanjay Joshi ◽  
Caroline Pogson ◽  
Stephen R Ebbs
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Lymph Nodes ◽  
Sentinel Node ◽  
Axillary Dissection ◽  
Frozen Section ◽  
Axillary Ultrasound ◽  
Node Biopsy ◽  
Operative Ultrasound

INTRODUCTION To ensure appropriate axillary surgery is performed at a single operation, we have sought to identify patients with involved nodes who might progress directly to axillary dissection. PATIENTS AND METHODS We evaluated pre-operative ultrasound of the axilla and intra-operative frozen section of sentinel lymph nodes over a 3-year period. Patients with clinical early breast cancer underwent axillary ultrasound. Abnormal nodes were defined as a cortex > 2.5 mm, loss of high echogenic medulla, and morphological changes. Any axilla containing a lymph node considered abnormal had ultrasound-directed fine needle aspiration (FNA) performed. Patients with positive cytology proceeded directly to axillary dissection. Patients with negative cytology and those with normal ultrasound proceeded to sentinel four-node biopsy using Patent Blue dye alone. A single sentinel node was evaluated by intra-operative frozen section. RESULTS A total of 311 patients underwent pre-operative ultrasound successfully, identifying 115 (77%) patients of the total 150 who were found to have positive lymph nodes. Overall, 196 patients underwent sentinel lymph node biopsy analysis intra-operatively. Of the 11 false negative cases in which the lymph node was found to be positive postoperatively, eight cases showed the single tested sentinel node contained cancer that was recognised on postoperative staining but not frozen section. In six, the deposit in the sentinel node was a micrometastasis. Three cases were found to contain cancer in the ‘non-sentinel' node; in all, this was micrometastatic disease. CONCLUSIONS This study confirms the value of pre-operative ultrasound and intra-operative frozen section examination of axillary nodes. Only 3.5% of patients required two operations.

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