scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofAframomum meleguetaMethanolic Seed Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Samuel Okwudili Onoja ◽  
Yusuf Ndukaku Omeh ◽  
Maxwell Ikechukwu Ezeja ◽  
Martins Ndubuisi Chukwu
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Seed Extract ◽  
Assay Method ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reactive Substance ◽  
Photometric Assay ◽  
Antioxidant Effects

Aframomum meleguetaSchum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract ofA. melegueta. The antioxidant effects were evaluated usingin vitro, 2, 2-diphenylpicrylhydrazine photometric assay andin vivoserum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed ofA. meleguetahas potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofSudarshanaPowder

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Weerakoon Achchige Selvi Saroja Weerakoon ◽  
Pathirage Kamal Perera ◽  
Dulani Gunasekera ◽  
Thusharie Sugandhika Suresh
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Trolox Equivalent ◽  
Antioxidant Effects

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

scholarly journals IN VIVO ANTIOXIDANT ACTIVITY OF LIMNOPHILA HETEROPHYLLA AND MICHELIA CHAMPACA

2017 ◽  
Vol 9 (12) ◽  
pp. 241 ◽  
Author(s):  
Raja S, ◽  
Ravindranadh K.
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reactive Substance ◽  
Reducing Ability ◽  
Liver And Kidney ◽  
Michelia Champaca ◽  
Different Doses

Objective: The present study was aimed at investigating the in-vivo antioxidant activity of the methanol extracts of Limnophila heterophylla and Michelia champaca leaves.Methods: Methanol extract of both plants were administered to rats separately at three different doses of 125, 250 and 500 mg/kg for 21 d to evaluate oxidative stress parameters such as ferric reducing ability of plasma (FRAP), thiobarbituric acid reactive substance (TBARS) and reduced glutathione (GSH) and to evaluate antioxidant enzyme levels of catalase (CAT) and superoxide dismutase (SOD).Results: The methanol extracts of both the plants significantly (p<0.05) elevated the ferric reducing ability of plasma (FRAP) on days 7, 14 and 21 of treatment. Significant (p<0.05) decrease of thiobarbituric acid reactive substance (TBARS) levels along with an increase in the superoxide dismutase (SOD) enzyme level in the liver and kidney at three different doses both the plants was observed. Treatment at a dose of 500 mg/kg b. w of both plants caused a significant increase only in the level of CAT in the liver and kidney. However, there was no significant effect of a thiobarbituric acid reactive substance (TBARS), superoxide dismutase (SOD) and catalase (CAT) in the heart and reduced glutathione (GSH) level in liver, heart and kidney at three different doses both the plants.Conclusion: These outcomes recommend that the leaves of Limnophila heterophylla and Michelia champaca have a potent antioxidant activity which may be responsible for some of its reported pharmacological actions. 

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

Antioxidant Properties of Deer Blood Hydrolysate and the Possible Mode of Action

2013 ◽  
Vol 634-638 ◽  
pp. 1435-1440 ◽  
Author(s):  
Shuai Wang ◽  
Li Cheng Zhong ◽  
Xue Chao Zhai ◽  
Dong Dong Yin ◽  
Xin Yu Wu
Keyword(s):  
Antioxidant Activity ◽  
Mode Of Action ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Thiobarbituric Acid ◽  
Degree Of Hydrolysis ◽  
Thiobarbituric Acid Reactive Substance ◽  
Hydrolysis Time ◽  
Reactive Substance ◽  
Tbars Values

Deer blood was hydrolyzed using Alcalase with hydrolysis time ranged form 0 to 6 h, and the degree of hydrolysis (DH) of protein hydrolysates increased with increasing hydrolysis time (P < 0.05). The reducing power, radicals scavenging activities and Cu2+-chelation ability of deer blood hydrolysate (DBH) significantly enhanced with increasing hydrolysis time (P < 0.05). The antioxidant activity of DBH, indicated by thiobarbituric acid-reactive substance (TBARS) values in a liposome-oxidizing system, increased with increasing DH (P < 0.05). The results indicated that antioxidant activity of DBH depended on hydrolysis time, and the hydrolyzed deer blood could be a potent food antioxidant.

Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia

1997 ◽  
Vol 272 (2) ◽  
pp. L294-L300 ◽  
Author(s):  
A. B. Al-Mehdi ◽  
H. Shuman ◽  
A. B. Fisher
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Reactive Oxygen ◽  
Thiobarbituric Acid ◽  
Conjugated Dienes ◽  
Thiobarbituric Acid Reactive Substance ◽  
Oxygen Species ◽  
Reactive Substance ◽  
Rat Lungs

Surface fluorometry with 40 microM hydroethidine (HE) as a probe was used to detect oxidant generation in isolated, ventilated rat lungs during lung ischemia. Ethidium fluorescence due to HE oxidation was continuously monitored with 470 nm excitation and 610 nm emission. Fluorescence increased with ischemia in O2-ventilated lungs [0.98 +/- 0.08 arbitrary fluorescence units (AFU)/min vs. 0.58 +/- 0.07 with control perfusion]. HE oxidation during ischemia was prevented by N2 ventilation but was unaltered by preperfusion with superoxide dismutase. Ethidium fluorescence in homogenate prepared from lungs subjected to 1 h of nonhypoxic ischemia was increased (16.8 +/- 1.5 vs. 9.8 +/- 0.4 AFU/mg protein in control) but was unchanged in lungs that had been N2 ventilated. Microfluorographs of HE perfused and fixed lung sections demonstrated marked generalized increases in ethidium fluorescence with ischemia compared with control perfusion. Ischemia resulted in significant increases in tissue thiobarbituric acid reactive substance (176 +/- 13 vs. 44 +/- 3 pmol/mg protein for control) and in lung conjugated dienes (0.90 +/- 0.07 vs. 0.48 +/- 0.06 U/mg protein for control), indicating peroxidation of lung lipids. These results indicate that lung ischemia leads to intracellular oxidant generation that can be continuously monitored by surface fluorometry.

scholarly journals Protective effects of molsidomine against doxorubicin-induced renal damage in rats

2016 ◽  
Vol 39 (1) ◽  
pp. 7 ◽  
Author(s):  
Fatih Oguz ◽  
Ali Beytur ◽  
Ediz Sarihan ◽  
Hilal K Oguz ◽  
Recep Bentli ◽  
...  
Keyword(s):  
Renal Damage ◽  
Reduced Glutathione ◽  
Kidney Injury ◽  
Thiobarbituric Acid ◽  
Kidney Damage ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Protective Effects ◽  
Reactive Substance ◽  
Group 4

Purpose: The purpose of this study was to investigate the therapeutic and protective effects of molsidomine (MLS) against doxorubicin (DOX)-induced renal damage in rats. Methods: Forty rats were randomly divided into five groups (control, MLS, DOX, DOX+MLS and MLS+DOX groups). Thiobarbituric acid reactive substance (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), nitric oxide (NO) and glutathione peroxidase (GPx) levels were determined from kidney tissues and blood urea nitrogen (BUN), creatinine (Cr) and albumin (Alb) levels also determined. Results: DOX treatment caused a significant increase in TBARS levels and a significant decrease in the GSH and CAT levels compared with the control group. In comparison, MLS administration before DOX injection caused a significant decrease in TBARS levels and also increases in GSH and CAT levels, whereas treatment of MLS after DOX injection did not show any beneficial effect on these parameters. All groups showed a significant increase in NO levels compared to the control group. There were no significant differences among the all groups for BUN and Cr levels. Serum level of Alb decreased in the DOX-treated groups when compared with control and MLS groups. The histopathological findings were in accordance with the biochemical results. MLS treatment reversed the DOX-induced kidney damage in group 4. MLS treatment before DOX injection exerted a protective effect against DOX-induced kidney damage. Conclusions: MLS shows promise as a possible therapeutic intervention for the prevention of kidney injury associated with DOX treatment. Additional studies are warranted.

scholarly journals Inhibitory Response ofRaphanus sativuson Lipid Peroxidation in Albino Rats

2008 ◽  
Vol 5 (1) ◽  
pp. 55-59 ◽  
Author(s):  
P. Chaturvedi
Keyword(s):  
Lipid Peroxidation ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Cumene Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Experimental Control ◽  
Reactive Substance

In the present study, inhibitory effect of the methanol extract ofRaphanus sativusroot on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg−1body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract ofR. sativusroot reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase.In vitroexperiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibitedin vitrocumene hydroperoxide induced lipid peroxidation.R. sativusinhibits lipid peroxidationin vivoandin vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

scholarly journals Aktivitas Antioksidan Ekstrak Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) Pada Asam Linoleat

Jurnal MIPA ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 86
Author(s):  
Jeremy Fransisco Pakasi ◽  
Lidya I Momuat ◽  
Harry S.J. Koleangan
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Total Phenolic ◽  
Thiobarbituric Acid Reactive Substance ◽  
Peroxide Formation ◽  
Reactive Substance ◽  
Percent Inhibition ◽  
Ferric Thiocyanate

Penelitian ini bertujuan untuk mempelajari aktivitas antioksidan eksrak tumbuhan suruhan Peperomia pellucida (L.) Kunth pada asam linoleat. Tumbuhan suruhan diekstrak dengan pelarut etanol 80% dan n-heksana dengan cara maserasi selama 48 jam. Ekstrak etanol dan n-heksana dari tumbuhan suruhan diukur kandungan total fenoliknya dengan metode Folin-ciocalteu, serta diuji aktivitas antioksidannya pada asam linoleat menggunakan metode Ferric Thiocyanate untuk menghitung persen penghambatan peroksida, dan metode Thiobarbituric Acid Reactive Substance untuk mengukur persen penghambatan pembentukan malonaldehida. Hasil penelitian kandungan total fenolik dalam ekstrak etanol dan n-heksana tumbuhan suruhan berturut-turut adalah 53.469 mg/kg dan 22.755 mg/kg. Aktivitas antioksidan dari ekstrak etanol tumbuhan suruhan dengan konsentrasi 100 dan 200 µg/mL dalam menghambat pembentukan peroksida berturut-turut sebesar 83.74% dan 88.80%, serta pembentukan malonaldehida sebesar 93.07% dan 93.96% pada asam linoleat.Sedangkan Aktivitas antioksidan dari ekstrak n-heksana tumbuhan suruhan dengan konsentrasi 100 dan 200 µg/mL dalam menghambat pembentukan peroksida berturut-turut sebesar 67.96% dan 73.18%, serta pembentukan malonaldehida sebesar 90.98% dan 92.00% pada asam linoleat.Penelitian ini menyimpulkan bahwa kandungan total fenolik ekstrak etanol tumbuhan suruhan lebih tinggi daripada ekstrak n-heksana, serta aktivitas antioksidan ekstrak etanol adalah yang terbaik dalam menghambat pembentukan peroksida dan malonaldehida pada asam linoleat.This reaserchwas aimed to study the antioxidant activity of Peperomia pellucida (L.) Kunth on linoleic acid. The plant was extracted with 80% ethanol and n-hexane solvent by maceration for 48 hours. The content of total phenolic was measured using the Folin-Ciocalteu method and the antioxidant activity of Peperomia p. wastested on linoleic acid using Ferric Thiocyanate method to calculate the percent inhibition of peroxide and using Thiobarbituric Acid Reactive Substance method for measuring the percent inhibition of malonaldehyde. Total phenolic content of the ethanol extract and the n-hexane extract of Peperomia p. were 53,469 mg/kg and22.755 mg/kg respectively. The antioxidant activities of ethanol extract of Peperomia p. with concentration of 100 and 200 μg/mL in inhibition of peroxide formation were 83,74% and 88,80%, and for malonaldehyde were 93,07% and 93,96% respectively. Whereasthose of n-hexana extracts with the same concentration inhibited 67.96% and 73.18% of peroxide formation, and 90.98% and 92.00% of malonaldehyde formation. Thus,Total content of phenolics of ethanol extract is higher than that of n-hexane extract, similarly the antioxidant activity of ethanol extract is the better in inhibiting the formation of peroxide and malonaldehyde in linoleic acid than that of n-hexana extract.

Mitochondrial lipid peroxidation and superoxide dismutase in rat hypertensive target organs

1995 ◽  
Vol 268 (4) ◽  
pp. H1418-H1421 ◽  
Author(s):  
T. Ohtsuki ◽  
M. Matsumoto ◽  
K. Suzuki ◽  
N. Taniguchi ◽  
T. Kamada
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Reactive Substance ◽  
Target Organs ◽  
Significant Difference ◽  
The Brain

Mitochondrial respiratory chains leak a large amount of superoxide anion radicals, which chain react with membrane phospholipid to develop lipid peroxidation. Manganese superoxide dismutase (MnSOD) is then inducible and catalyzes superoxide detoxification within mitochondria. We examined mitochondrial thiobarbituric acid-reactive substance, an end product of lipid peroxidation, and MnSOD concentration in hypertensive target organs of spontaneously hypertensive and deoxycorticosterone acetate salts-induced hypertensive rats. Normotensive rats showed significant increases in thiobarbituric acid-reactive substance and MnSOD in the brain as they matured. Mature spontaneously hypertensive and induced hypertensive rats showed a marked elevation of lipid peroxidation but no increase in superoxide dismutase in the brain. The heart and kidney presented no significant difference of lipid peroxidation and superoxide dismutase among strains, ages, and treatments. Abnormal mitochondrial metabolism of oxygen radicals was observed selectively in the brain during hypertension and may contribute to mitochondrial injury and lead to neuronal degeneration or susceptibility to brain ischemia in mature hypertensive rats.

Sleep deprivation reduces total plasma homocysteine levels in rats

2002 ◽  
Vol 80 (3) ◽  
pp. 193-197 ◽  
Author(s):  
A C de Oliveira ◽  
V D'Almeida ◽  
D C Hipólide ◽  
J N Nobrega ◽  
S Tufik
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Thiobarbituric Acid ◽  
Plasma Homocysteine ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Total Plasma ◽  
Reactive Substance ◽  
Systemic Oxidative Stress ◽  
Physiological Processes

Hyperhomocysteinemia has been associated with pathological and stressful conditions and is a risk factor for cardiovascular disease. Since sleep deprivation is a stressful condition that is associated with disruption of various physiological processes, we investigated whether it would also be associated with increases in plasma homocysteine levels. Further, since hyperhomocysteinemia may promote oxidative stress, and we had previously found evidence of oxidative stress in brain following sleep deprivation, we also searched for evidence of systemic oxidative stress by measuring glutathione and thiobarbituric acid reactive substance levels. Rats were sleep deprived for 96 h using the platform technique. A group was killed after sleep deprivation and another two groups were allowed to undergo sleep recovery for 24 or 48 h. Contrary to expectation, plasma homocysteine was reduced in sleep-deprived rats as compared with the control group and did not revert to normal levels after 24 or 48 h of sleep recovery. A trend was observed towards decreased glutathione and increased thiobarbituric acid reactive substance levels in sleep-deprived rats. It is possible that the observed decreases in homocysteine levels may represent a self-correcting response to depleted glutathione in sleep-deprived animals, which would contribute to the attenuation of the deleterious effects of sleep deprivation.Key words: sleep deprivation, homocysteine, oxidative stress, glutathione, rats.

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