Sleep deprivation reduces total plasma homocysteine levels in rats

2002 ◽  
Vol 80 (3) ◽  
pp. 193-197 ◽  
Author(s):  
A C de Oliveira ◽  
V D'Almeida ◽  
D C Hipólide ◽  
J N Nobrega ◽  
S Tufik
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Thiobarbituric Acid ◽  
Plasma Homocysteine ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Total Plasma ◽  
Reactive Substance ◽  
Systemic Oxidative Stress ◽  
Physiological Processes

Hyperhomocysteinemia has been associated with pathological and stressful conditions and is a risk factor for cardiovascular disease. Since sleep deprivation is a stressful condition that is associated with disruption of various physiological processes, we investigated whether it would also be associated with increases in plasma homocysteine levels. Further, since hyperhomocysteinemia may promote oxidative stress, and we had previously found evidence of oxidative stress in brain following sleep deprivation, we also searched for evidence of systemic oxidative stress by measuring glutathione and thiobarbituric acid reactive substance levels. Rats were sleep deprived for 96 h using the platform technique. A group was killed after sleep deprivation and another two groups were allowed to undergo sleep recovery for 24 or 48 h. Contrary to expectation, plasma homocysteine was reduced in sleep-deprived rats as compared with the control group and did not revert to normal levels after 24 or 48 h of sleep recovery. A trend was observed towards decreased glutathione and increased thiobarbituric acid reactive substance levels in sleep-deprived rats. It is possible that the observed decreases in homocysteine levels may represent a self-correcting response to depleted glutathione in sleep-deprived animals, which would contribute to the attenuation of the deleterious effects of sleep deprivation.Key words: sleep deprivation, homocysteine, oxidative stress, glutathione, rats.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

scholarly journals Protective effects of molsidomine against doxorubicin-induced renal damage in rats

2016 ◽  
Vol 39 (1) ◽  
pp. 7 ◽  
Author(s):  
Fatih Oguz ◽  
Ali Beytur ◽  
Ediz Sarihan ◽  
Hilal K Oguz ◽  
Recep Bentli ◽  
...  
Keyword(s):  
Renal Damage ◽  
Reduced Glutathione ◽  
Kidney Injury ◽  
Thiobarbituric Acid ◽  
Kidney Damage ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Protective Effects ◽  
Reactive Substance ◽  
Group 4

Purpose: The purpose of this study was to investigate the therapeutic and protective effects of molsidomine (MLS) against doxorubicin (DOX)-induced renal damage in rats. Methods: Forty rats were randomly divided into five groups (control, MLS, DOX, DOX+MLS and MLS+DOX groups). Thiobarbituric acid reactive substance (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), nitric oxide (NO) and glutathione peroxidase (GPx) levels were determined from kidney tissues and blood urea nitrogen (BUN), creatinine (Cr) and albumin (Alb) levels also determined. Results: DOX treatment caused a significant increase in TBARS levels and a significant decrease in the GSH and CAT levels compared with the control group. In comparison, MLS administration before DOX injection caused a significant decrease in TBARS levels and also increases in GSH and CAT levels, whereas treatment of MLS after DOX injection did not show any beneficial effect on these parameters. All groups showed a significant increase in NO levels compared to the control group. There were no significant differences among the all groups for BUN and Cr levels. Serum level of Alb decreased in the DOX-treated groups when compared with control and MLS groups. The histopathological findings were in accordance with the biochemical results. MLS treatment reversed the DOX-induced kidney damage in group 4. MLS treatment before DOX injection exerted a protective effect against DOX-induced kidney damage. Conclusions: MLS shows promise as a possible therapeutic intervention for the prevention of kidney injury associated with DOX treatment. Additional studies are warranted.

scholarly journals The role of oxidative stress markers in pregnancy induced hypertension

2017 ◽  
Vol 70 (1-2) ◽  
pp. 33-38
Author(s):  
Dragica Draganovic ◽  
Branka Cancarevic-Djajic ◽  
Dragica Jojic
Keyword(s):  
Oxidative Stress ◽  
Pregnant Women ◽  
Thiobarbituric Acid ◽  
Oxidative Stress Marker ◽  
Thiobarbituric Acid Reactive Substance ◽  
Pregnancy Induced Hypertension ◽  
Reactive Substance ◽  
Stress Marker ◽  
Induced Hypertension

Introduction. This article investigated the role of oxidative stress in the etiology of pregnancy induced hypertension. The aim of this study was to determine the degree of oxidative stress, and the level of thiobarbituric acid reactive substance in the blood of pregnant women with and without pregnancy induced hypertension and to correlate these parameters with clinical parameters during pregnancy and delivery. Material and Methods. This prospective study was performed at the University Clinical Centre of the Republic of Srpska. It included 200 pregnant women - 100 with pregnancy induced hypertension, and 100 healthy normotensive pregnant women between 28 to 40 weeks of gestation. Results. Pregnant women with pregnancy induced hypertension had significantly higher median levels of oxidative stress marker: thiobarbituric acid reactive substance of 36.7 ?mol compared to the control group of 13.2 ?mol. Pregnant women with pregnancy induced hypertension presenting with complications had significantly higher thiobarbituric acid reactive substance mean levels of 41.6 ?mol compared with pregnant women without complications. The highest thiobarbituric acid reactive substance level of 43.9 ?mol was found in pregnant women with Hemolysis, Elevated, Liver Ensimes, Low Plateles syndrome. Conclusion. The study showed that thiobarbituric acid reactive substance, as an oxidative stress marker, may be used in clinical practice in the assessment of the severity of complications and as an indicator for timely delivery in women with pregnancy induced hypertension. Further studies and a larger study sample of pregnant women with severe hypertension are necessary to confirm this conclusion.

scholarly journals Inhibitory Response ofRaphanus sativuson Lipid Peroxidation in Albino Rats

2008 ◽  
Vol 5 (1) ◽  
pp. 55-59 ◽  
Author(s):  
P. Chaturvedi
Keyword(s):  
Lipid Peroxidation ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Cumene Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Experimental Control ◽  
Reactive Substance

In the present study, inhibitory effect of the methanol extract ofRaphanus sativusroot on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg−1body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract ofR. sativusroot reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase.In vitroexperiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibitedin vitrocumene hydroperoxide induced lipid peroxidation.R. sativusinhibits lipid peroxidationin vivoandin vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

scholarly journals Effects of Phenolic Compounds of Fermented Thai Indigenous Plants on Oxidative Stress in Streptozotocin-Induced Diabetic Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Chaiyavat Chaiyasut ◽  
Winthana Kusirisin ◽  
Narissara Lailerd ◽  
Peerasak Lerttrakarnnon ◽  
Maitree Suttajit ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Indigenous Plants ◽  
Glucose Levels ◽  
Abnormal Glucose Metabolism

We investigated the effects of antioxidant activity of fermentation product (FP) of five Thai indigenous products on oxidative stress in Wistar rats with streptozotocin (STZ)-induced diabetes type II. The rats were fed with placebo and with the FP (2 and 6 mL/kg body weight/day) for 6 weeks. Rutin, pyrogallol and gallic acid were main compounds found in the FP. Plasma glucose levels in diabetic rats receiving the higher dose of the FP increased less when compared to the diabetic control group as well as the group receiving the lower FP dose (13.1%, 29%, and 21.1%), respectively. A significant dose-dependent decrease in plasma levels of thiobarbituric acid reactive substance (P<.05) was observed. In addition, the doses of 2 and 6 mL FP/kg/day decreased the levels of erythrocyte ROS in diabetic rats during the experiment, but no difference was observed when compared to the untreated diabetic rat group. Results imply that FP decreased the diabetes-associated oxidative stress to a large extent through the inhibition of lipid peroxidation. The FP also improved the abnormal glucose metabolism slightly but the difference was not statistically significant. Thus, FP may be a potential therapeutic agent by reducing injury caused by oxidative stress associated with diabetes.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofAframomum meleguetaMethanolic Seed Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Samuel Okwudili Onoja ◽  
Yusuf Ndukaku Omeh ◽  
Maxwell Ikechukwu Ezeja ◽  
Martins Ndubuisi Chukwu
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Seed Extract ◽  
Assay Method ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reactive Substance ◽  
Photometric Assay ◽  
Antioxidant Effects

Aframomum meleguetaSchum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract ofA. melegueta. The antioxidant effects were evaluated usingin vitro, 2, 2-diphenylpicrylhydrazine photometric assay andin vivoserum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed ofA. meleguetahas potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

scholarly journals REVERSAL OF CISPLATIN-INDUCED MEMORY IMPAIRMENT BY GALLIC ACID IN EXPERIMENTAL RAT MODEL

2020 ◽  
pp. 153-156
Author(s):  
Sucharita Patel ◽  
Chandan HM ◽  
Krishna KL ◽  
Nandini HS ◽  
Abhinav Raj Ghosh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Locomotor Activity ◽  
Gallic Acid ◽  
Rat Model ◽  
Memory Impairment ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Albino Rats ◽  
Thiobarbituric Acid Reactive Substance

Objective: The objective of the study was to evaluate the protective effect of gallic acid on cisplatin-induced memory impairment (MI) in the rat model. Methods: Five groups of Wistar albino rats (n=6) were employed and the duration of the study was 22 days, excluding the pre-treatment period. Animals were pretreated with gallic acid for a period of 5 days and continued daily (200 mg/kg) for 22 days. The cisplatin (3 mg/kg) was given once in a week for 3 consecutive weeks to induce MI, whereas donepezil was used as standard. The evaluation was done by a change in body weight, memory activity by Morris water maze (MWM), locomotor activity by actophotometer, antioxidant activity by thiobarbituric acid reactive substance (TBARS), glutathione (GSH), and estimation of acetylcholinesterase (AChE) activity in brain homogenate. Results: Administration of cisplatin has induced MI by increasing in escape latency time, decrease in time spent in the target quadrant in the MWM task and it was reversed by gallic acid treatment. Decreased locomotor activity by cisplatin was also increased by gallic acid when tested by actophotometer. Cisplatin administration has induced oxidative stress by increasing TBARS and decreasing GSH levels. Gallic acid due to its proven antioxidant activity reversed the effects of cisplatin. The AChE level was significantly increased in the control group, whereas treatment groups have shown a decrease in AChE level. Conclusion: Gallic acid may serve as a primary agent to treat the cognitive impairment and oxidative stress associated memory dysfunctions. However, more extensive studies are needed before utilization in the clinical trial.

scholarly journals Oxidative Stress in Animal Models of Acute and Chronic Renal Failure

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Marianna Gyurászová ◽  
Alexandra Gaál Kovalčíková ◽  
Emese Renczés ◽  
Katarína Kmeťová ◽  
Peter Celec ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Kidney Disease ◽  
Kidney Injury ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Antioxidant Power ◽  
Antioxidative Status ◽  
Systemic Oxidative Stress ◽  
Male Wistar Rats ◽  
Potential Biomarkers

Introduction. Kidney disease is a worldwide health and economic burden, with rising prevalence. The search for biomarkers for earlier and more effective disease screening and monitoring is needed. Oxidative stress has been linked to both, acute kidney injury (AKI) and chronic kidney disease (CKD). The aim of our study was to investigate whether the concentrations of systemic markers of oxidative stress and antioxidant status are affected by AKI and CKD, and to identify potential biomarkers. Methods. In adult male Wistar rats, AKI was induced by bilateral nephrectomy, and CKD was induced by 5/6 nephrectomy. Blood was collected 48 hours after surgery in AKI and 6 months after surgery in CKD. Advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs), fructosamine, total antioxidant capacity (TAC), and ferric reducing antioxidant power (FRAP) were measured. Results. Impaired renal function was confirmed by high concentrations of plasma creatinine and urea in AKI and CKD animals. AOPP and fructosamine were higher by 100% and 54% in AKI, respectively, and by 100% and 199% in CKD, respectively, when compared to corresponding control groups. Similarly, there was approximately a twofold increase in AGEs (by 92%) and TAC (by 102%) during AKI. In CKD, concentrations of FRAP, as an antioxidative status marker, were doubled (by 107%) when compared to the control group, but concentration of TAC, another marker of antioxidative status, did not differ between the groups. Conclusions. AKI and CKD led to increased systemic oxidative stress. AOPP and fructosamine could be considered potential biomarkers for both, acute and chronic kidney damage. On the other hand, AGEs, TAC, and FRAP seem to be disease specific, which could help to differentiate between acute and chronic kidney injuries. However, this needs further validation in clinical studies.

scholarly journals Thiobarbituric acid reactive substances as marker of oxidative stress in pregnancies with pre-eclampsia

2011 ◽  
Vol 64 (7-8) ◽  
pp. 377-380 ◽  
Author(s):  
Aleksandra Novakov-Mikic ◽  
Snezana Brkic ◽  
Daniela Maric ◽  
Bojan Sekulic ◽  
Aleksandar Cetkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Screening Method ◽  
Systolic Arterial Pressure ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reactive Substance ◽  
To Come ◽  
Tbars Assay

Pre-eclampsia is characterized by increased lipid peroxidation and diminished antioxidant capacity. The aim of the study was to establish concentration of thiobarbituric acid reactive substances as a marker of lipid peroxidation in normal pregnancies and in pregnancies complicated with pre-eclampsia, and to estimate the possibility of using thiobarbituric acid reactive substances as a screening method for development of pre-eclampsia. The study was conducted at the Department of Obstetrics and Gynaecology, Clinical Centre of Vojvodina. The study included 57 singleton pregnancies, gestation >24 weeks, of which 29 were healthy pregnancies and 28 were with pre-eclampsia, defined as systolic arterial pressure of >90 mmHg, diastolic of >145 mmHg, and 24h proteinuria of >300mg. Thiobarbituric acid reactive substances concentrations evaluated by malondialdehyde equivalent standards (OxiSelect? TBARS Assay Kit (malondialdehyde Quantitation), Cell Biolabs? OxiSelect?) showed that oxidative stress was more evident in the group with pre-eclampsia, though not statistically significant (p= 0.107). There was no correlation of thiobarbituric acid reactive substance levels with gestation in either group. The differences between the level of thiobarbituric acid reactive substance concentrations in pre-eclampsia and healthy pregnancies indicate the possibility of using thiobarbituric acid reactive substances as a screening tool for the development of pre-eclampsia. Further studies with larger numbers of patients are needed in order to come to final conclusions.

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