scholarly journals Molecular Cloning and Bioinformatics Analysis of a New Plasma Membrane Na+/H+Antiporter Gene from the HalophyteKosteletzkya virginica

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hongyan Wang ◽  
Xiaoli Tang ◽  
Chuyang Shao ◽  
Hongbo Shao ◽  
Honglei Wang
Keyword(s):  
Plasma Membrane ◽  
Bioinformatics Analysis ◽  
Transmembrane Protein ◽  
Terminal Region ◽  
Reading Frame ◽  
Kosteletzkya Virginica ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Salt Overly Sensitive ◽  
Plant Plasma Membrane

A new plasma membrane Na+/H+antiporter gene (named asKvSOS1) was cloned from the halophyteKosteletzkya virginicaby reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72–82% identity at the peptide level with other plant plasma membrane Na+/H+antiporters.

scholarly journals Cloning, sequencing and expression of rat liver pyruvate carboxylase

1996 ◽  
Vol 316 (2) ◽  
pp. 631-637 ◽  
Author(s):  
Sarawut JITRAPAKDEE ◽  
Grant W. BOOKER ◽  
A. Ian CASSADY ◽  
John C. WALLACE
Keyword(s):  
Rat Liver ◽  
Pyruvate Carboxylase ◽  
Untranslated Region ◽  
Northern Analysis ◽  
Reading Frame ◽  
Liver Cdna Library ◽  
Lactating Mammary Gland ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Sequencing And Expression

Overlapping clones encoding rat liver pyruvate carboxylase (PC) have been isolated by screening a liver cDNA library and by performing rapid amplification of cDNA ends polymerase chain reaction on total liver RNA. The sequence of rat PC cDNA contains an open reading frame of 3537 nucleotides encoding a polypeptide of 1178 amino acids with a calculated Mr of 129848. This is flanked by a 5′ untranslated region of 66 bp and a 3′ untranslated region of 421 bp including the poly(A) tail. The inferred protein sequence is 96.6% identical with mouse and 96.3% identical with human PCs, 68.4% identical with mosquito PC and 53.5% identical with yeast PC isoenzymes PC1 and PC2. On the basis of partial proteolysis and sequence homology with PC from other organisms (yeast, mosquito, mouse and human) and with other biotin enzymes, three functional domains, namely the biotin carboxylation domain, the transcarboxylation domain and the biotinyl domain, have been identified. Comparison with the known structure of the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase [Waldrop, Rayment and Holden (1994) Biochemistry 33, 10249–10256] highlights the functional importance of 11 highly conserved residues. Northern analysis revealed that PC mRNA is highly expressed in rat liver, kidney, adipose tissue and brain, moderately expressed in heart, adrenal gland and lactating mammary gland, and expressed at a low level in spleen and skeletal muscle.

scholarly journals The 14-3-3 protein interacts directly with the C-terminal region of the plant plasma membrane H(+)-ATPase.

1997 ◽  
Vol 9 (10) ◽  
pp. 1805-1814 ◽  
Author(s):  
T Jahn ◽  
A T Fuglsang ◽  
A Olsson ◽  
I M Brüntrup ◽  
D B Collinge ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Terminal Region ◽  
Plant Plasma Membrane

scholarly journals Activation of Plant Plasma Membrane H+-ATPase by 14-3-3 Proteins Is Negatively Controlled by Two Phosphorylation Sites within the H+-ATPase C-terminal Region

2008 ◽  
Vol 284 (7) ◽  
pp. 4213-4221 ◽  
Author(s):  
Geoffrey Duby ◽  
Wojciech Poreba ◽  
Dominik Piotrowiak ◽  
Krzysztof Bobik ◽  
Rita Derua ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Terminal Region ◽  
Phosphorylation Sites ◽  
Plant Plasma Membrane

The 14-3-3 Protein Interacts Directly with the C-Terminal Region of the Plant Plasma Membrane H + -ATPase

1997 ◽  
Vol 9 (10) ◽  
pp. 1805 ◽  
Author(s):  
Thomas Jahn ◽  
Anja T. Fuglsang ◽  
Anne Olsson ◽  
Ines Maria Bruntrup ◽  
David B. Collinge ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Terminal Region ◽  
Plant Plasma Membrane

scholarly journals Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors

2016 ◽  
Vol 141 (5) ◽  
pp. 407-413 ◽  
Author(s):  
Hai-nan Liu ◽  
Jian-rong Feng ◽  
Xiao-fang Liu ◽  
Wen-hui Li ◽  
Wen-juan Lv ◽  
...  
Keyword(s):  
Structural Characteristics ◽  
Prunus Armeniaca ◽  
Cauliflower Mosaic Virus ◽  
Expression Vectors ◽  
Variable Regions ◽  
Reading Frame ◽  
Hypervariable Regions ◽  
Polymerase Chain ◽  
Rapid Amplification ◽  
Virus Promoter

Three kinds of expression vectors of a pollen-S determinant were constructed to provide a reference for molecular breeding of self-compatible (SC) Prunus species. An S-haplotype-specific F-box (SFB) protein gene from the ‘Xiaobaixing’ apricot (Prunus armeniaca) was cloned by reverse transcription polymerase chain reaction (RT-PCR) and 3′-rapid-amplification of cDNA ends (3′-RACE). A 1136-bp sequence complementary to the 3′-end of the cDNA (GenBank accession number KP938528.2) with a 912-bp complete open reading frame (ORF) was obtained. The deduced amino acid sequence contained an F-box domain, two variable regions, and two hypervariable regions with structural characteristics similar to SFB in other Rosaceae plants. Sense, antisense, and RNA interference (RNAi) vectors for SFB were constructed by enzyme restriction. The target fragment was restricted using the corresponding restriction enzyme and then directionally inserted between the 35S cauliflower mosaic virus promoter and the nopaline synthase terminator (NOS-ter) of the expression vector pCAMBIA-35S-MCS-NOS-NPTII. The intron-containing hairpin RNA (ihpRNA) was obtained by fusion PCR. The constructed vectors were transferred into Agrobacterium tumefaciens strain LBA4404 by freezing/thawing. The RNAi vector of SFB was also transformed in tobacco (Nicotiana tabacum). The successful construction of these three expression vectors provides a basis for transforming ‘Xiaobaixing’ apricot and the breeding of SC Prunus cultivars.

scholarly journals Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata)

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Wenchao Yu ◽  
Baolu Zhang ◽  
Hongce Song ◽  
Rui Zhan ◽  
Lingling Li ◽  
...  
Keyword(s):  
Transmembrane Protein ◽  
Expression Profiles ◽  
Preliminary Investigation ◽  
Pomacea Canaliculata ◽  
Apple Snail ◽  
Growth Phases ◽  
Reading Frame ◽  
Golden Apple Snail ◽  
Rapid Amplification ◽  
Body Whorl

Abstract Background Growth hormone inducible transmembrane protein (GHITM) is a highly conserved transmembrane protein. This study was conducted to investigate the role of GHITM gene in the apoptosis and growth of the golden apple snail Pomacea canaliculate. Results The complete cDNA of this gene was cloned using the rapid amplification of cDNA ends (RACE) method and subjected to bioinformatics analysis. The full-length cDNA was 2242 bp, including an open reading frame of 1021 bp that encoded a protein of 342 amino acid residues. The mRNA expression profiles of GHITM gene in different tissues (liver, kidney, gonad and foot) and different growth phases (6-months old and 2-years old) showed that it was expressed in various tissues and different growth phases. Silencing of the GHITM gene by RNAi (RNA interference) experiments revealed that the GHITM gene possibly plays a role in inhibiting apoptosis through detecting the Caspase (Cysteine-requiring Aspartate Protease)-3 activity. In addition, the aperture width and body whorl length of the snail was significantly affected by RNAi, suggesting that this gene plays a significant role in promoting the growth of the organism. Conclusions These results demonstrated that the GHITM gene was involved in apoptosis and growth in golden apple snail.

scholarly journals Molecular Cloning and Expression Analysis of Cu/Zn SOD Gene from Gynura bicolor DC.

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xin Xu ◽  
Zhi-fang Yu ◽  
Jing Kong ◽  
Mei-jun Yi ◽  
Chen Huan ◽  
...  
Keyword(s):  
Bioinformatics Analysis ◽  
Higher Plants ◽  
Full Length ◽  
Cloning And Expression ◽  
Future Research ◽  
Mikania Micrantha ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Gynura Bicolor ◽  
Rapid Amplification

Superoxide dismutase is an important antioxidant enzyme extensively existing in eukaryote, which scavenges reactive oxygen species (ROS) and plays an essential role in stress tolerance of higher plants. A full-length cDNA encoding Cu/Zn SOD was cloned from leaves of Gynura bicolor DC. by rapid amplification of cDNA ends (RACE). The full-length cDNA of Cu/Zn SOD is 924 bp and has a 681 bp open reading frame encoding 227 amino acids. Bioinformatics analysis revealed that belonged to the plant SOD super family. Cu/Zn SODs of the Helianthus annuus, Mikania micrantha, and Solidago canadensis var. scabra all have 86% similarity to the G. bicolor Cu/Zn SOD. Analysis of the expression of Cu/Zn SOD under different treatments revealed that Cu/Zn SOD was a stress-responsive gene, especially to 1-MCP. It indicates that the Cu/Zn SOD gene would be an important gene in the resistance to stresses and will be helpful in providing evidence for future research on underlying molecular mechanism and choosing proper postharvest treatments for G. bicolor.

scholarly journals Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Martha Alicia Ballinas-Verdugo ◽  
Rogelio Frank Jiménez-Ortega ◽  
Eduardo Martínez-Martínez ◽  
Nancy Rivas ◽  
Erick Abraham Contreras-López ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Extracellular Vesicles ◽  
Bioinformatics Analysis ◽  
Chronic Phase ◽  
Heart Tissue ◽  
Detailed Knowledge ◽  
Total Plasma ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Relevant Finding

Abstract Background Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. MicroRNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-β signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction. Results Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated. Conclusions The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.

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