scholarly journals Molecular Cloning and Expression Analysis of Cu/Zn SOD Gene from Gynura bicolor DC.

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xin Xu ◽  
Zhi-fang Yu ◽  
Jing Kong ◽  
Mei-jun Yi ◽  
Chen Huan ◽  
...  
Keyword(s):  
Bioinformatics Analysis ◽  
Higher Plants ◽  
Full Length ◽  
Cloning And Expression ◽  
Future Research ◽  
Mikania Micrantha ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Gynura Bicolor ◽  
Rapid Amplification

Superoxide dismutase is an important antioxidant enzyme extensively existing in eukaryote, which scavenges reactive oxygen species (ROS) and plays an essential role in stress tolerance of higher plants. A full-length cDNA encoding Cu/Zn SOD was cloned from leaves of Gynura bicolor DC. by rapid amplification of cDNA ends (RACE). The full-length cDNA of Cu/Zn SOD is 924 bp and has a 681 bp open reading frame encoding 227 amino acids. Bioinformatics analysis revealed that belonged to the plant SOD super family. Cu/Zn SODs of the Helianthus annuus, Mikania micrantha, and Solidago canadensis var. scabra all have 86% similarity to the G. bicolor Cu/Zn SOD. Analysis of the expression of Cu/Zn SOD under different treatments revealed that Cu/Zn SOD was a stress-responsive gene, especially to 1-MCP. It indicates that the Cu/Zn SOD gene would be an important gene in the resistance to stresses and will be helpful in providing evidence for future research on underlying molecular mechanism and choosing proper postharvest treatments for G. bicolor.

Molecular cloning, expression profiling and functional analyses of a cDNA encoding isopentenyl diphosphate isomerase from Gossypium barbadense

2009 ◽  
Vol 29 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Yechun Wang ◽  
Chengxiang Qiu ◽  
Fei Zhang ◽  
Binhui Guo ◽  
Zhiqi Miao ◽  
...  
Keyword(s):  
Mevalonate Pathway ◽  
Plant Defence ◽  
Gossypium Barbadense ◽  
Full Length ◽  
Isopentenyl Diphosphate ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Dimethylallyl Diphosphate ◽  
Rapid Amplification ◽  
High Level

Gossypol, a type of plant defence sesquiterpenoid phytoalexin, is synthesized from the MEP (2C-methyl-D-erythritol 4-phosphate) and MVA (mevalonate) pathway in the isoprenoid biosynthetic system. The key step is the isomerization of IPP (isopentenyl diphosphate) to DMAPP (dimethylallyl diphosphate), which is catalysed by IPI (IPP isomerase; EC 5.3.3.2). A full-length cDNA encoding IPI (designated GbIPI) was cloned from Gossypium barbadense by RACE (rapid amplification of cDNA ends). The full-length cDNA of GbIPI was 1205 bp and contained a 906 bp ORF (open reading frame) encoding a protein of 302 amino acids, with a predicted molecular mass of 34.39 kDa and an isoelectric point of 6.07. Amino acid sequence analysis revealed that the GbIPI has a high level of similarity to other IPIs. Southern-blot analysis revealed that GbIPI belongs to a small gene family. Expression analysis indicated that GbIPI expression is highest in stems, followed by leaves, and is lowest in roots, and that the expression of GbIPI could be induced by Verticillium dahliae Kleb, MeJA (methyl jasmonate) and SA (salicylic acid). The functional colour assay indicated that GbIPI could accelerate the accumulation of β-carotene in Escherichia coli transformants. The cloning and functional analysis of GbIPI will be useful in increasing understanding of the role of IPI in isoprenoid biosynthesis at the molecular level.

Molecular Cloning of a HMG-CoA Reductase Gene from Eucommia ulmoides Oliver

2006 ◽  
Vol 26 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Jihong Jiang ◽  
Guoyin Kai ◽  
Xiaoying Cao ◽  
Fengmei Chen ◽  
Dongning He ◽  
...  
Keyword(s):  
Bioinformatic Analysis ◽  
Tissue Expression ◽  
Full Length ◽  
Eucommia Ulmoides ◽  
Phylogenetic Tree Analysis ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Calculated Molecular Weight ◽  
Rapid Amplification ◽  
Coa Reductase

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate, which is the first committed step in the pathway for isoprenoid biosynthesis in plants. A full-length cDNA encoding HMGR (designated as EuHMGR, GenBank Accession No. AY796343) was isolated from Eucommia ulmoides by rapid amplification of cDNA ends (RACE). The full-length cDNA of EuHMGR comprises 2281 bp with a 1770-bp open reading frame (ORF) encoding a 590-amino-acid polypeptide with two trans-membrane domains revealed by bioinformatic analysis. Molecular modeling showed that EuHMGR is a new HMGR with a spatial structure similar to other plant HMGRs. The deduced protein has an isoelectric point (pI) of 6.89 and a calculated molecular weight of about 63 kDa. Sequence comparison analysis showed that EuHMGR had highest homology to HMGR from Hevea brasiliensis. As expected, phylogenetic tree analysis indicated that EuHMGR belongs to plant HMGR group. Tissue expression pattern analysis showed that EuHMGR is strongly expressed in the leaves and stems whereas it is only poorly expressed in the roots, which implies that EuHMGR may be a constitutively expressing gene. Functional complementation of EuHMGR in HMGR-deficient mutant yeast JRY2394 demonstrated that EuHMGR mediates the mevalonate biosynthesis in yeast.

Cloning and characterisation of the gene encoding HMG-CoA reductase from Taxus media and its functional identification in yeast

2004 ◽  
Vol 31 (1) ◽  
pp. 73 ◽  
Author(s):  
Zhihua Liao ◽  
Qiumin Tan ◽  
Yourong Chai ◽  
Kaijing Zuo ◽  
Min Chen ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Catalytic Domain ◽  
Bioinformatic Analysis ◽  
Full Length ◽  
Functional Identification ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Taxus Media ◽  
Taxol Biosynthesis ◽  
Coa Reductase

In plants, the first committed step in the pathway for biosynthesis of isoprenoids is catalysed by 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC: 1.1.1.34). Here we report for the first time the cloning of a full-length cDNA encoding HMGR (Tm–HMGR) from a taxol-producing gymnosperm, Taxus media Rehder. The full-length cDNA of Tm–HMGR (GenBank accession number: AY277740) was 2307 base pairs (bp), with a 1791-bp open reading frame (ORF) encoding a 596-amino-acid polypeptide. Bioinformatic analysis revealed that Tm–HMGR contained two trans-membrane domains and a catalytic domain, and showed high homology to other plant HMGRs. Phylogenetic analysis indicated that Tm–HMGR was more ancient than other plant HMGRs. The structural modelling showed that Tm–HMGR had the typical spatial structure of HMGRs whose catalytic domains could be folded and divided into three spatial domains, L-domain, N-domain and S-domain. Southern blot analysis revealed that Tm–HMGR belonged to a small HMGR gene family. Northern blot analysis showed that Tm–HMGR was expressed in roots, stems and needles, with higher expression in stems and needles than in roots. Functional complementation of Tm–HMGR in a HMGR-deficient mutant yeast demonstrated that Tm–HMGR mediated the biosynthesis of mevalonate and provided the general precursor for taxol biosynthesis.

Cloning, characterisation and expression profile of kisspeptin1 and the kisspeptin1 receptor in the hypothalamic–pituitary–ovarian axis of Chinese alligator Alligator sinensis during the reproductive cycle

2020 ◽  
Vol 32 (8) ◽  
pp. 792 ◽  
Author(s):  
Ruidong Zhang ◽  
Haitao Nie ◽  
Shulong Duan ◽  
Peng Yan ◽  
Ali Izaz ◽  
...  
Keyword(s):  
Amino Acid ◽  
Quantitative Polymerase Chain Reaction ◽  
Reproductive Period ◽  
Full Length ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Cdna Sequences ◽  
Acid Protein ◽  
Amino Acid Precursor ◽  
Alligator Sinensis

Kisspeptin1 (Kiss1), a product of the Kiss1 gene, plays an important role in the regulation of reproduction in vertebrates by activating the Kiss1 receptor (Kiss1R) and its coexpression with gonadotrophin-releasing hormone (GnRH) in GnRH neurons. The purpose of this study was to clone the Kiss1 and Kiss1R genes found in the brain of Alligator sinensis and to explore their relationship with reproduction. The full-length cDNA of Kiss1 is 816bp, the open reading frame (ORF) is 417bp and the gene encodes a 138-amino acid precursor protein. The full-length cDNA of Kiss1R is 2348bp, the ORF is 1086bp and the gene encodes a 361-amino acid protein. Quantitative polymerase chain reaction showed that, except for Kiss1R expression in the hypothalamus, the expression of Kiss1 and Kiss1Rduring the reproductive period of A. sinensis was higher than that in the hypothalamus, pituitary gland and ovary during the hibernation period. The changes in GnRH2 mRNA in the hypothalamus were similar to those of GnRH1 and peaked during the reproductive period. This study confirms the existence of Kiss1 and Kiss1R in A. sinensis and the findings strongly suggest that Kiss1 and Kiss1R may participate in the regulation of GnRH secretion in the hypothalamus of alligators during the reproductive period. Furthermore, this is the first report of the full-length cDNA sequences of Kiss1 and Kiss1R in reptiles.

scholarly journals Identification, Characterization, and Expression Analysis of the TaNF-YB3 Gene from Triticum aestivum

2011 ◽  
Vol 39 (2) ◽  
pp. 254
Author(s):  
Xiuchun DONG ◽  
Zhiyuan CHENG ◽  
Shuhan CHENG ◽  
Feng XU ◽  
Yanrong AN ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Full Length ◽  
Day Length ◽  
Reading Frame ◽  
Full Length Cdna ◽  
Rhythmic Expression ◽  
Binding Factor ◽  
A Subunit ◽  
Family Gene ◽  
Ccaat Box

A full-length cDNA encoding a nuclear factor-YB (NF-YB)/HAP3/CCAAT binding factor-A (CBF-A) subunit of a CCAAT-box binding complex, designated as TaNF-YB3 was isolated from Triticum aestivum. Sequence analysis indicated that the full-length cDNA was 809 bp long, including an open reading frame (ORF) of 597 bp, which encoded a deduced polypeptide of 199 amino acids and is located in chromosome 3D. The deduced protein contained conserved structural domains and showed high identity to other plant NF-YBs. TaNF-YB3 was expressed in various organs, especially in the leaves and stamens; it was also regulated by salt, mannitol, abscisic acid, wounding, and cold. Moreover, TaNF-YB3 was down-regulated by short days and vernalization, and sensitive to the transfer of day length. It was mainly induced by light and exhibited a similar diurnal rhythmic expression pattern with the CCT-domain family gene VRN2 (TaZCCT1 and TaZCCT2), but not with CO (WCO1 and TaHd1). Overall, the results suggested that TaNF-YB3, aside from having a role in regulating day length and vernalization responses, might integrate signals from other environmental stresses to perform its functions in winter wheat adaptability and development.

scholarly journals Cloning and bioinformatics analysis of a full-length cDNA of porcine CR1-like gene

2014 ◽  
Vol 46 (11) ◽  
pp. 997-1000 ◽  
Author(s):  
Jing Cheng ◽  
Junbing Jiang ◽  
Junxing Zhao ◽  
Zhirui Wang ◽  
Yaogui Sun ◽  
...  
Keyword(s):  
Bioinformatics Analysis ◽  
Full Length ◽  
Full Length Cdna

Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus)

2019 ◽  
Author(s):  
Zhilong Tian ◽  
Yuqin Wang ◽  
Huibin Shi ◽  
Zhibo Wu ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Phylogenetic Analyses ◽  
Expression Patterns ◽  
Amino Acid Sequences ◽  
Full Length ◽  
Capra Hircus ◽  
Reading Frame ◽  
Relative Molecular Weight ◽  
Rapid Amplification ◽  
And Function

To further to understand the structure and function of the TAC1 gene, we cloned the full-length cDNAs of the TAC1 genes from goat by rapid amplification of cDNA ends-PCR and the qRT-PCR was used to analyze the TAC1 mRNA expression patterns of goat various tissues. The full-length cDNA of goat TAC1 was 1176 bp, with a 339 bp open reading frame encoding 112 amino acids. The amino acid sequence analysis revealed that goat TAC1 gene encoded a water-drain protein and its relative molecular weight and isoelectric point was 13,012.86 Da and 6.29 respectively. Alignment and phylogenetic analyses revealed that their amino acid sequences were highly similar to those of other vertebrates. TAC1 expression of the goat of the brain, cerebellum, medulla oblongata, heart, liver, spleen, lung, kidney, uterus, ovaries. These results serve as a foundation for further study on the Capra hircus TAC1 gene.

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