scholarly journals A New Multichannel Spectral Imaging Laser Scanning Confocal Microscope

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yunhai Zhang ◽  
Bian Hu ◽  
Yakang Dai ◽  
Haomin Yang ◽  
Wei Huang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Spectral Imaging ◽  
Biological Tissues ◽  
Confocal Microscope ◽  
Fluorescent Labeling ◽  
Confocal Imaging ◽  
Laser Scanning Confocal Microscope ◽  
3 Dimensional ◽  
Key Technologies

We have developed a new multichannel spectral imaging laser scanning confocal microscope for effective detection of multiple fluorescent labeling in the research of biological tissues. In this paper, the design and key technologies of the system are introduced. Representative results on confocal imaging, 3-dimensional sectioning imaging, and spectral imaging are demonstrated. The results indicated that the system is applicable to multiple fluorescent labeling in biological experiments.

A video rate laser scanning confocal microscope

2008 ◽  
Author(s):  
Hongzhou Ma ◽  
James Jiang ◽  
Hongwu Ren ◽  
Alex E. Cable
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

scholarly journals Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Karen J Thompson ◽  
Cynthia M Harley ◽  
Grant M Barthel ◽  
Mark A Sanders ◽  
Karen A Mesce
Keyword(s):  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Laser Scanning ◽  
Energy State ◽  
Confocal Microscope ◽  
Fluorescent Imaging ◽  
Cell Labeling ◽  
Resolving Power ◽  
Noble Metal Nanoparticles ◽  
Laser Scanning Confocal Microscope

The staining of neurons with silver began in the 1800s, but until now the great resolving power of the laser scanning confocal microscope has not been utilized to capture the in-focus and three-dimensional cytoarchitecture of metal-impregnated cells. Here, we demonstrate how spectral confocal microscopy, typically reserved for fluorescent imaging, can be used to visualize metal-labeled tissues. This imaging does not involve the reflectance of metal particles, but rather the excitation of silver (or gold) nanoparticles and their putative surface plasmon resonance. To induce such resonance, silver or gold particles were excited with visible-wavelength laser lines (561 or 640 nm), and the maximal emission signal was collected at a shorter wavelength (i.e., higher energy state). Because the surface plasmon resonances of noble metal nanoparticles offer a superior optical signal and do not photobleach, our novel protocol holds enormous promise of a rebirth and further development of silver- and gold-based cell labeling protocols.

Measurement characteristics analysis and test of 3D laser scanning confocal microscope

2018 ◽  
Vol 47 (8) ◽  
pp. 817005
Author(s):  
崔建军 Cui Jianjun ◽  
杜 华 Du Hua ◽  
朱小平 Zhu Xiaoping ◽  
薛 梓 Xue Zi ◽  
闫勇刚 Yan Yonggang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
3D Laser Scanning ◽  
Laser Scanning Confocal Microscope ◽  
Characteristics Analysis

Edge enhancement of weak-phase object in laser scanning confocal microscope

2005 ◽  
Author(s):  
Masahide Itoh ◽  
Shin Uematsu ◽  
Hiroshi Ishiwata ◽  
Toyohiko Yatagai
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Edge Enhancement ◽  
Phase Object ◽  
Laser Scanning Confocal Microscope ◽  
Weak Phase

Variable-ambient scanning stage for a laser scanning confocal microscope

2003 ◽  
Vol 74 (10) ◽  
pp. 4366-4368 ◽  
Author(s):  
D. J. Sirbuly ◽  
J. P. Schmidt ◽  
M. D. Mason ◽  
M. A. Summers ◽  
S. K. Buratto
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

Confocal Imaging of Both Mitochondrial and Cytosolic Free Ca2+ in Cardiac Myocytes Co-Loaded with Rhod 2 and Fluo 3: Inhibition by Ruthenium Red of Mitochondrial but not Cytosolic Ca2+ Transients

1998 ◽  
Vol 4 (S2) ◽  
pp. 448-449
Author(s):  
Donna R. Trollinger ◽  
Wayne E. Cascio ◽  
John J. Lemasters
Keyword(s):  
Electrical Stimulation ◽  
Cardiac Myocytes ◽  
Nutrient Medium ◽  
Laser Scanning ◽  
Ruthenium Red ◽  
Confocal Imaging ◽  
Adult Rabbit ◽  
Green Fluorescence ◽  
Laser Scanning Confocal Microscope ◽  
Red Fluorescence

Previously, we showed rapid mitochondrial Ca2+ transients in adult rabbit cardiac myocytes during the contractile cycle. Ruthenium red, an inhibitor of mitochondrial Ca2+ uptake by the Ca2+ uniporter, inhibits mitochondrial Ca2+ transients in adult rabbit cardiac myocytes during electrical stimulation. Here, we extend this finding to show that ruthenium red inhibition is specific for mitochondrial Ca2+ transients and not cytosolic Ca2+ transients.Ca 2+-tolerant adult rabbit cardiac myocytes were isolated by collagenase and hyaluronidase digestion and loaded in the cold with 2.5 μM Rhod 2-AM for 30 minutes. Subsequently, the cells were incubated in nutrient medium at 37°C for 4-6 hours during which time cytosolic but not mitochondrial Rhod 2 was lost. Subsequently, the myocytes were loaded with 10 μM Fluo 3-AM for 15 minutes at 37°C, conditions that lead to predominantly cytosolic localization of Fluo 3. In sequential scans, the red fluorescence of Rhod 2 and the green fluorescence of Fluo 3 were imaged using a Zeiss LSM 410 laser scanning confocal microscope.

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