Edge enhancement of weak-phase object in laser scanning confocal microscope

2005 ◽  
Author(s):  
Masahide Itoh ◽  
Shin Uematsu ◽  
Hiroshi Ishiwata ◽  
Toyohiko Yatagai
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Edge Enhancement ◽  
Phase Object ◽  
Laser Scanning Confocal Microscope ◽  
Weak Phase

A video rate laser scanning confocal microscope

2008 ◽  
Author(s):  
Hongzhou Ma ◽  
James Jiang ◽  
Hongwu Ren ◽  
Alex E. Cable
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

scholarly journals Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Karen J Thompson ◽  
Cynthia M Harley ◽  
Grant M Barthel ◽  
Mark A Sanders ◽  
Karen A Mesce
Keyword(s):  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Laser Scanning ◽  
Energy State ◽  
Confocal Microscope ◽  
Fluorescent Imaging ◽  
Cell Labeling ◽  
Resolving Power ◽  
Noble Metal Nanoparticles ◽  
Laser Scanning Confocal Microscope

The staining of neurons with silver began in the 1800s, but until now the great resolving power of the laser scanning confocal microscope has not been utilized to capture the in-focus and three-dimensional cytoarchitecture of metal-impregnated cells. Here, we demonstrate how spectral confocal microscopy, typically reserved for fluorescent imaging, can be used to visualize metal-labeled tissues. This imaging does not involve the reflectance of metal particles, but rather the excitation of silver (or gold) nanoparticles and their putative surface plasmon resonance. To induce such resonance, silver or gold particles were excited with visible-wavelength laser lines (561 or 640 nm), and the maximal emission signal was collected at a shorter wavelength (i.e., higher energy state). Because the surface plasmon resonances of noble metal nanoparticles offer a superior optical signal and do not photobleach, our novel protocol holds enormous promise of a rebirth and further development of silver- and gold-based cell labeling protocols.

Measurement characteristics analysis and test of 3D laser scanning confocal microscope

2018 ◽  
Vol 47 (8) ◽  
pp. 817005
Author(s):  
崔建军 Cui Jianjun ◽  
杜 华 Du Hua ◽  
朱小平 Zhu Xiaoping ◽  
薛 梓 Xue Zi ◽  
闫勇刚 Yan Yonggang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
3D Laser Scanning ◽  
Laser Scanning Confocal Microscope ◽  
Characteristics Analysis

Variable-ambient scanning stage for a laser scanning confocal microscope

2003 ◽  
Vol 74 (10) ◽  
pp. 4366-4368 ◽  
Author(s):  
D. J. Sirbuly ◽  
J. P. Schmidt ◽  
M. D. Mason ◽  
M. A. Summers ◽  
S. K. Buratto
Keyword(s):  
Laser Scanning ◽  
Confocal Microscope ◽  
Laser Scanning Confocal Microscope

scholarly journals Prospective Study of the Diagnostic Accuracy of the In Vivo Laser Scanning Confocal Microscope for Severe Microbial Keratitis

Ophthalmology ◽  
2016 ◽  
Vol 123 (11) ◽  
pp. 2285-2293 ◽  
Author(s):  
Jaya D. Chidambaram ◽  
Namperumalsamy V. Prajna ◽  
Natasha L. Larke ◽  
Srikanthi Palepu ◽  
Shruti Lanjewar ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Prospective Study ◽  
Laser Scanning ◽  
Confocal Microscope ◽  
Microbial Keratitis ◽  
Laser Scanning Confocal Microscope
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