scholarly journals Paprika Pigments Attenuate Obesity-Induced Inflammation in 3T3-L1 Adipocytes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Hayato Maeda ◽  
Shuuichi Saito ◽  
Nozomi Nakamura ◽  
Takashi Maoka
Keyword(s):  
Mrna Expression ◽  
Chronic Inflammation ◽  
Lipid Level ◽  
Blood Lipid ◽  
Macrophage Cell ◽  
Lipid Disorders ◽  
Chemotactic Protein ◽  
Adiponectin Mrna ◽  
Necrosis Factor ◽  
Resistin Mrna

Obesity is related to various diseases, such as diabetes, hyperlipidemia, and hypertension. Adipocytokine, which is released from adipocyte cells, affects insulin resistance and blood lipid level disorders. Further, adipocytokine is related to chronic inflammation in obesity condition adipocyte cells. Paprika pigments (PPs) contain large amounts of capsanthin and capsorubin. These carotenoids affect the liver and improve lipid disorders of the blood. However, how these carotenoids affect adipocyte cells remains unknown. Present study examined the effects of PP on adipocytokine secretion, which is related to improvement of metabolic syndrome. In addition, suppressive effects of PP on chronic inflammation in adipocyte cells were analyzed using 3T3-L1 adipocyte cells and macrophage cell coculture experiments. PP promoted 3T3-L1 adipocyte cells differentiation upregulated adiponectin mRNA expression and secretion. Further, coculture of adipocyte and macrophage cells treated with PP showed suppressed interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1), and resistin mRNA expression, similarly to treatment with troglitazone, which is a PPARγ ligand medicine. Conclusion. These results suggest that PP ameliorates chronic inflammation in adipocytes caused by obesity. PP adjusts adipocytokine secretion and might, therefore, affect antimetabolic syndrome diseases.

scholarly journals Skeletal muscle deiodinase type 2 regulation during illness in mice

2009 ◽  
Vol 203 (2) ◽  
pp. 263-270 ◽  
Author(s):  
J Kwakkel ◽  
H C van Beeren ◽  
M T Ackermans ◽  
M C Platvoet-ter Schiphorst ◽  
E Fliers ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Chronic Inflammation ◽  
Severity Of Illness ◽  
Camp Pathway ◽  
Factor Α ◽  
Socs Box ◽  
Serum Thyroid Hormones ◽  
Necrosis Factor

We have previously shown that skeletal muscle deiodinase type 2 (D2) mRNA (listed as Dio2 in MGI Database) is upregulated in an animal model of acute illness. However, human studies on the expression of muscle D2 during illness report conflicting data. Therefore, we evaluated the expression of skeletal muscle D2 and D2-regulating factors in two mouse models of illness that differ in timing and severity of illness: 1) turpentine-induced inflammation, and 2) Streptococcus pneumoniae infection. During turpentine-induced inflammation, D2 mRNA and activity increased compared to pair-fed controls, most prominently at day 1 and 2, whereas after S. pneumoniae infection D2 mRNA decreased. We evaluated the association of D2 expression with serum thyroid hormones, (de-)ubiquitinating enzymes ubiquitin-specific peptidase 33 and WD repeat and SOCS box-containing 1 (Wsb1), cytokine expression and activation of inflammatory pathways and cAMP pathway. During chronic inflammation the increased muscle D2 expression is associated with the activation of the cAMP pathway. The normalization of D2 5 days after turpentine injection coincides with increased Wsb1 and tumor necrosis factor α expression. Muscle interleukin-1β (Il1b) expression correlated with decreased D2 mRNA expression after S. pneumoniae infection. In conclusion, muscle D2 expression is differentially regulated during illness, probably related to differences in the inflammatory response and type of pathology. D2 mRNA and activity increases in skeletal muscle during the acute phase of chronic inflammation compared to pair-fed controls probably due to activation of the cAMP pathway. In contrast, muscle D2 mRNA decreases 48 h after a severe bacterial infection, which is associated with local Il1b mRNA expression and might also be due to diminished food-intake.

scholarly journals Peculiarities of lipid disorders in blood serum in chronic process of cerebral atherosclerosis

1997 ◽  
Vol XXIX (3-4) ◽  
pp. 69-71
Author(s):  
B. A. Osetrov ◽  
А. A. Komissarenko ◽  
L. V. Salycheva
Keyword(s):  
Blood Serum ◽  
Coronary Atherosclerosis ◽  
Lipid Level ◽  
Blood Lipid ◽  
Brain Circulation ◽  
Integral Evaluation ◽  
Lipid Disorders ◽  
Cerebral Atherosclerosis ◽  
Lipid Spectrum

In 107 patients with chronic insufficiency of brain circulation of atherosclerotic genesis (of different expressiveness) lipid level of blood serum is determined, cholesterine coefficient of atherogenecity and supposed by the authors coefficient of lipid disorders are calculated. The obtained results have been compared with data of analogous examination of 78 patients, having atherosclerotic stenocardia of tension. Integral evaluation of blood lipid spectrum with regard to its peculiarities allowed to determine that expressiveness of lipid disorders in patients with cerebral atherosclerosis is not reduced, and is even increased in coronary atherosclerosis.

Peripheral mononuclear TNF-α, visfatin, and resistin mRNA expression in overweight women with type 2 diabetes

2007 ◽  
Vol 2 (04) ◽  
Author(s):  
P Tsiotra ◽  
C Tsigos ◽  
E Yfanti ◽  
E Anastasiou ◽  
SA Raptis
Keyword(s):  
Type 2 Diabetes ◽  
Mrna Expression ◽  
Overweight Women ◽  
Tnf Α ◽  
Resistin Mrna

scholarly journals Leptin and Adiponectin mRNA Expression From the Adipose Tissue Surrounding the Adrenal Neoplasia

2015 ◽  
Vol 100 (1) ◽  
pp. E101-E104 ◽  
Author(s):  
Claudio Letizia ◽  
Luigi Petramala ◽  
Cira Rosaria Tiziana Di Gioia ◽  
Caterina Chiappetta ◽  
Laura Zinnamosca ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mrna Expression ◽  
Adiponectin Mrna

scholarly journals Cloudy Apple Juice Fermented by Lactobacillus Prevents Obesity Via Modulating Gut Microbiota and Protecting Intestinal Tract Health

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 971
Author(s):  
Mengzhen Han ◽  
Meina Zhang ◽  
Xiaowei Wang ◽  
Xue Bai ◽  
Tianli Yue ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Apple Juice ◽  
Intestinal Tract ◽  
Lipid Level ◽  
Blood Lipid ◽  
Obese Mice ◽  
Research Findings ◽  
Gut Microbiota Composition ◽  
Blood Lipid Levels

Obesity and hyperglycemia are two serious chronic diseases that are increasing in incidence worldwide. This research aimed to develop a fermented cloudy apple juice with good hyperglycemia intervention activities. Here, cloudy apple juice (CAJ), cloudy apple juice rich in polyphenols (CAJP) and fermented cloudy apple juice rich in polyphenols (FCAJP) were prepared sequentially, and then the effects of the three apple juices on weight, lipid level, gut microbiota composition and intestinal tract health were evaluated for obese mice induced by a high-fat diet. The research findings revealed that the FCAJP showed potential to inhibit the weight gain of mice, reduce fat accumulation, and regulate the blood lipid levels of obese mice by decreasing the ratio of the Firmicutes/Bacteroidotas, improving the Sobs, Ace, and Chao indexes of the gut microbiota and protecting intestinal tract health. In addition, the FCAJP augmented the abundance of Akkermansia and Bacteroides, which were positively related to SCFAs in cecal contents. This study inferred that FCAJP could be developed as a healthy food for preventing obesity and hyperglycemia.

Fish oil enhances macrophage tumor necrosis factor-alpha mRNA expression at the transcriptional level

Metabolism ◽  
1995 ◽  
Vol 44 (6) ◽  
pp. 800-805 ◽  
Author(s):  
Hernan R. Chang ◽  
Denis Arsenijevic ◽  
Ion-Rusan Vladoianu ◽  
Lucien Girardier ◽  
Abdul G. Dulloo
Keyword(s):  
Tumor Necrosis Factor ◽  
Fish Oil ◽  
Mrna Expression ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Transcriptional Level ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

scholarly journals MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results

2021 ◽  
Vol 22 (14) ◽  
pp. 7241
Author(s):  
Piotr K. Krajewski ◽  
Weronika Szukała ◽  
Agata Lichawska-Cieślar ◽  
Łukasz Matusiak ◽  
Jolanta Jura ◽  
...  
Keyword(s):  
Western Blot ◽  
Mrna Expression ◽  
Hidradenitis Suppurativa ◽  
Skin Lesions ◽  
Healthy Controls ◽  
Lesional Skin ◽  
Chemotactic Protein ◽  
Healthy Control ◽  
Mrna And Protein Expression ◽  
Skin Biopsies

The pathogenesis of hidradenitis suppurativa (HS) is yet to be fully understood. However, inflammation is a key element in the development of skin lesions. The aim of this study was to evaluate the expression of monocyte chemotactic protein-1-induced protein-1 (MCPIP1) in the skin of patients suffering from HS. Skin biopsies of 15 patients with HS and 15 healthy controls were obtained and processed for immunohistochemistry, western blot, and real time PCR. The highest mean MCPIP1 mRNA expression was found in the inflammatory lesional skin of HS patients. It was significantly higher than MCPIP1 mRNA expression in the biopsies from both healthy controls and non-lesional skin of HS patients. Western blot analysis indicated that expression of MCPIP1 was elevated within both lesional and non-lesional skin compared to the healthy control. The increased MCPIP1 mRNA and protein expression level in HS lesions may indicate its possible role in the disease pathogenesis.

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