Characterization of the photoautotrophic algal and bacterial communities in a large, shallow, subtropical lake using HPLC-PDA based pigment analysis

1998 ◽  
Vol 55 (1) ◽  
pp. 206-219 ◽  
Author(s):  
Alan D Steinman ◽  
Karl E Havens ◽  
J William Louda ◽  
Nancy M Winfree ◽  
Earl W Baker
Keyword(s):  
Water Column ◽  
Chlorophyll A ◽  
High Performance ◽  
Principal Component ◽  
Open Water ◽  
High Performance Liquid Chromatographic ◽  
Lake Okeechobee ◽  
Pheophytin A ◽  
Pigment Analysis ◽  
Liquid Chromatographic

Pigment abundances of the oxygenic and anoxygenic photoautotrophic communities from sediments and the water column in Lake Okeechobee, Florida, were estimated using reverse-phase high-performance liquid chromatographic (RP-HPLC) and photodiode array (PDA) UV/VIS (350-800 nm) spectrophotometric analyses. Thirty lipophilic pigments were identified and measured in the samples, with the most abundant overall (sediment and open-water samples combined) being chlorophyll a (38.1%), fucoxanthin (12.6%), pheophytin a (7.6%), zeaxanthin (6.6%), and pyropheophytin a (3.6%). Relative abundance of chlorophyll a was greater in the water column than in the sediments (58.3 versus 24.3% of all pigments) whereas pheophytin a comprised 9.1% of the total pigments in the sediments but only 3.7% of the total pigments in the water column. Principal component analysis (PCA) separated the sediment samples from those collected in the water column; this discrimination appears to be a function of pigment integrity in that sediment assemblages had much greater relative abundances of degraded pigments. Different regions of the lake were weakly separated by PCA based on pigments. The relatively weak degree of separation may reflect the overwhelming abundance of chlorophyll a at all sites. Overall, the pigment assemblage in Lake Okeechobee suggests cyanobacteria-diatom dominance. Out of 65 sampling events, pigments from anoxygenic photoautotrophs (e.g., bacteriochlorophylls) were detected 17 times but accounted for >20% of total chlorophyll only five times. Bacteriochlorophylls were observed only in the sediments and were most abundant during June and September, when winds were calm and temperatures warm, and at relatively shallow sites.

scholarly journals Evaluation of the Influence of Sulfur-FumigatedPaeoniae Radix Albaon the Quality of Si Wu Tang by Chromatographic and Chemometric Analysis

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Ke Pei ◽  
Hao Cai ◽  
Yu Duan ◽  
Feng-Xian Qiao ◽  
Si-Cong Tu ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Great Influence ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Fingerprint ◽  
Paeoniae Radix ◽  
Liquid Chromatographic

An accurate and reliable method of high-performance liquid chromatographic fingerprint combining with multi-ingredient determination was developed and validated to evaluate the influence of sulfur-fumigatedPaeoniae Radix Albaon the quality and chemical constituents of Si Wu Tang. Multivariate data analysis including hierarchical cluster analysis and principal component analysis, which integrated with high-performance liquid chromatographic fingerprint and multi-ingredient determination, was employed to evaluate Si Wu Tang in a more objective and scientific way. Interestingly, in this paper, a total of 37 and 36 peaks were marked as common peaks in ten batches of Si Wu Tang containing sun-driedPaeoniae Radix Albaand ten batches of Si Wu Tang containing sulfur-fumigatedPaeoniae Radix Alba, respectively, which indicated the changed fingerprint profile of Si Wu Tang when containing sulfur-fumigated herb. Furthermore, the results of simultaneous determination for multiple ingredients showed that the contents of albiflorin and paeoniflorin decreased significantly (P<0.01) and the contents of gallic acid andZ-ligustilide decreased to some extent at the same time when Si Wu Tang contained sulfur-fumigatedPaeoniae Radix Alba. Therefore, sulfur-fumigation processing may have great influence on the quality of Chinese herbal prescription.

scholarly journals Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Polygoni Perfoliati Herba

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Li Tian ◽  
Yang Zhao ◽  
Xin Zhou ◽  
Hua-Guo Chen ◽  
Chao Zhao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
Principal Component ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Fingerprints ◽  
Peritoneal Permeability ◽  
Anti Inflammatory ◽  
Relationship Of ◽  
Liquid Chromatographic ◽  
The Relationship

Polygoni Perfoliati Herba is widely used in China with antibacterium, anti-inflammatory, expectorant, antitumor, and antivirus activities. To reveal the mechanisms of the activities of Polygoni Perfoliati Herba, the relationship between the fingerprinting profile and its bioactivities was investigated. In the present study, high-performance liquid chromatographic (HPLC) fingerprinting method was developed. The established method was applied to analyze 51 batches of Polygoni Perfoliati Herba samples collected from different locations or in different harvesting times in China. Chemometrics, including similarity analysis, hierarchical clustering analysis, and principal component analysis, were used to express their similarities. It was found that similarity values of the samples were in the range of 0.432–0.998. The results of analgesic tests indicated that Polygoni Perfoliati Herba could significantly inhibit pain induced by hot plate and acetic acid in mice. The results of anti-inflammatory tests showed that Polygoni Perfoliati Herba had good anti-inflammatory effects (P< 0.01) in two models including dimethyl benzene-induced ear edema and acetic acid-induced peritoneal permeability in mice. Combining the results from chromatographic fingerprints with those from bioactivities, we found that seven peaks from Polygoni Perfoliati Herba were mainly responsible for analgesic and anti-inflammatory activities.

scholarly journals Chromatographic Fingerprint Analysis of Pycnogenol® Dietary Supplements

2009 ◽  
Vol 92 (2) ◽  
pp. 624-632 ◽  
Author(s):  
Pei Chen ◽  
Fenhong Song ◽  
Long-Ze Lin
Keyword(s):  
Dietary Supplements ◽  
High Performance ◽  
Principal Component ◽  
The United States ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprints ◽  
Peak Areas ◽  
Liquid Chromatographic

Abstract The bark of maritime pine (Pinus pinaster Aiton) has been widely used as a remedy for various degenerative diseases. A standard high-performance liquid chromatographic (HPLC) procedure for Pycnogenol® analysis is a method specified in the United States Pharmacopeia (USP) monograph, which requires measurement of peak areas and identification of four components of the extract: caffeic acid, catechin, ferulic acid, and taxifolin. In this study, a fingerprint analysis using an HPLC method based on the USP monograph has been developed to provide additional qualitative information for the analysis of Pycnogenol-containing dietary supplements (PDS). Twelve commercially available PDS samples were purchased and analyzed along with a standard Pycnogenol extract. Their chromatographic fingerprints were analyzed using principal component analysis. The results showed that two of the samples were not consistent with the standard reference Pycnogenol extract. One contained other active ingredients in addition to Pycnogenol, and the other may have resulted from a quality control issue in manufacturing.

scholarly journals Comparison of High-Performance Liquid Chromatographic and Thin-Layer Chromatographic Methods for Determination of Aloin in Herbal Products Containing Aloe vera

2008 ◽  
Vol 91 (6) ◽  
pp. 1265-1270 ◽  
Author(s):  
Rosalba Ramírez Durón ◽  
Luca Ceniceros Almaguer ◽  
Norma Cecilia Cavazos Rocha ◽  
Perla Giovanna Silva Flores ◽  
Noem Waksman De Torres
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Aloe Vera ◽  
Principal Component ◽  
High Performance Liquid Chromatographic ◽  
Herbal Products ◽  
Liquid Chromatographic Method ◽  
Cheap Method ◽  
Liquid Chromatographic

Abstract Aloe vera is a medicinal plant used worldwide to treat a variety of conditions and, as such, has important commercial value. Aloin is a principal component of aloe vera leaves and is used for quality control of products containing it. A semiquantitative thin-layer chromatographic (TLC) method for determining the concentration of aloin in aloe-based products was validated. The results were similar to those of a validated high-performance liquid chromatographic method; therefore, TLC, which is a simple, sensitive, specific, rapid, and cheap method, may be ideal for use in any laboratory for routine analysis of commercial products containing aloe vera.

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