scholarly journals Spatial-Frequency Azimuthally Stable Cartography of Biological Polycrystalline Networks

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
V. A. Ushenko ◽  
N. D. Pavlyukovich ◽  
L. Trifonyuk
Keyword(s):  
Spatial Frequency ◽  
Optical Activity ◽  
Polarization Plane ◽  
Biological Tissues ◽  
Uterine Wall ◽  
Beam Polarization ◽  
Linear Birefringence ◽  
Histological Sections ◽  
The Matrix ◽  
Anisotropic Structures

A new azimuthally stable polarimetric technique processing microscopic images of optically anisotropic structures of biological tissues histological sections is proposed. It has been used as a generalized model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity. The matrix elementM44has been chosen as the main information parameter, whose value is independent of the rotation angle of both sample and probing beam polarization plane. For the first time, the technique of concerted spatial-frequency filtration has been used in order to separate the manifestation of linear birefringence and optical activity. Thereupon, the method of azimuthally stable spatial-frequency cartography of biological tissues histological sections has been elaborated. As the analyzing tool, complex statistic, correlation, and fractal analysis of coordinate distributions ofM44element has been performed. The possibility of using the biopsy of the uterine wall tissue in order to differentiate benign (fibromyoma) and malignant (adenocarcinoma) conditions has been estimated.

Stokes Mueller Treatment of Artifacts in Natural Raman Optical Activity

1987 ◽  
Vol 41 (2) ◽  
pp. 295-307 ◽  
Author(s):  
L. Hecht ◽  
B. Jordanov ◽  
B. Schrader
Keyword(s):  
Raman Scattering ◽  
Optical Activity ◽  
Linear Birefringence ◽  
Chiral Molecules ◽  
Sample Cell ◽  
Raman Optical Activity ◽  
Angle Scattering ◽  
The Matrix ◽  
Phase Sensitive ◽  
First Time

A description of Raman scattering based on the theory by Barron and on Stokes Mueller formalism was developed into a uniform mathematical calculus such that Raman scattering can now be easily represented by a simple phenomenological matrix. This matrix, the Mueller matrix of a modulator driven by a square-wave voltage, and the matrix for a laser regarded as a fictitious polarizer are presented for the first time. With the use of these three matrices and the ones for the analyzer, the polarizer, the linearly birefringent sample cell, the circularly birefringent sample, and the partially polarizing detector, the main reasons for the appearance of artifacts in Raman optical activity have been identified. On the basis of a calculation for right-angle scattering of an isotropic solution of chiral molecules, sources of artifacts appear to be the linear birefringence of the cell walls and of the modulator material. Phase-sensitive detection is found to observe Raman optical activity with a minimal number of artifacts.

scholarly journals Laser polarization-variable autofluorescence of the network of optically anisotropic biological tissues: Diagnostics and differentiation of early stages of cancer ofcervix uteri

2014 ◽  
Vol 07 (03) ◽  
pp. 1450024 ◽  
Author(s):  
Yu. A. Ushenko ◽  
M. I. Sidor ◽  
N. Pashkovskaia ◽  
G. D. Koval ◽  
Yu. F. Marchuk ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Cervix Uteri ◽  
Linear Birefringence ◽  
Laser Polarization ◽  
Histological Sections ◽  
Early Stages ◽  
Operational Characteristics ◽  
Carcinoma Of Cervix ◽  
Endometrium Biopsy ◽  
Sensitivity Specificity

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and differentiation of early stages of cancer: Dysplasia — cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (fibrillar, porphyrin) networks. The technique of polarization-variable laser autofluorescence was developed and experimentally tested. The objective criteria (statistical moments) of differentiation of histological sections autofluorescent images of endometrium biopsy and cytological smears of it mucous coat were defined. The operational characteristics (sensitivity, specificity, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical efficiency.

scholarly journals Interference-contrast optical activity: a new technique for probing the chirality of anisotropic samples and more

2020 ◽  
Vol 7 (5) ◽  
pp. 192201 ◽  
Author(s):  
R. P. Cameron ◽  
U. Vogl ◽  
N. Trautmann
Keyword(s):  
Pharmaceutical Industry ◽  
Optical Activity ◽  
Optical Rotation ◽  
New Technique ◽  
Future Research ◽  
Linear Birefringence ◽  
New Class ◽  
Potential Applications ◽  
A New Technique ◽  
Chiral Crystals

We introduce interference-contrast optical activity (ICOA) as a new technique for probing the chirality of anisotropic samples and more. ICOA could underpin a new class of ‘chiral microscopes’, with potential applications spanning the range of chirality and beyond. Two possible versions of ICOA are described explicitly; one designed to probe the optical rotation of a transparent sample regardless of the sample’s linear birefringence (ICOA-OR) and another designed to probe gradients in the optical rotation of a transparent sample (ICOA-GOR). Simulated results for α -quartz lead us to suggest that ICOA-GOR might be applied to help monitor the growth of chiral crystals in the pharmaceutical industry. Possible directions for future research are highlighted.

scholarly journals Differential diagnostics of the presence of damage by the method of digital polarization mapping of microsocopy images of hystological sections of human organs

2021 ◽  
pp. 70-78
Author(s):  
Oleksandra Litvinenko ◽  
Oleh Vanchulyak ◽  
Irina Soltys ◽  
Olga Mikhailova ◽  
Artem Motrich
Keyword(s):  
Lung Tissue ◽  
Experimental Testing ◽  
Biological Tissues ◽  
Differential Diagnostics ◽  
Time Interval ◽  
Diagnostic Efficiency ◽  
Internal Organs ◽  
Microscopic Images ◽  
Histological Sections ◽  
Ellipticity Of Polarization

The article presents the results of experimental testing of methods of azimuthal-invariant polarization mapping of microscopic images of samples of histological sections of the myocardium and lung tissue; time monitoring of changes in the magnitude of statistical moments of the 1-4th orders, characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of myocardial and lung tissue samples with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping azimuth maps and polarization ellipticity of microscopic images. Aim of the work. Development of a digital histology technique for samples of human internal organs. Materials and methods. The object of the study was the histology of samples of human internal organs (myocardium, lung tissue) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples from those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the method of digital polarization mapping of microscopic images of histological sections of biological tissues of human internal organs. Results. Revealed the following ranges ramp variation quantity statistics polarizing digital histology and accuracy of the limitation of damage: a. Arts azimuth polarization microscopic image of a magnification ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 55-60 min), maps of ellipticity of polarization of microscopic images with a magnification of ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 65-75 min). Conclusions. Scenarios of changes in the statistical structure of maps of azimuth and ellipticity of polarization of microscopic images of histological sections of human internal organs have been determined - with an increase in the duration of damage, the value of the mean and variance decreases, the asymmetry and kurtosis increase.

scholarly journals Methods and means of asymutal-invariant muller matrix polyarimetry of optical and anisotropic biological layers

2019 ◽  
Keyword(s):  
Differential Diagnostics ◽  
Experimental Development ◽  
Coronary Insufficiency ◽  
Theoretical Substantiation ◽  
Histological Sections ◽  
The Matrix ◽  
Acute Coronary Insufficiency ◽  
Matrix Mapping ◽  
Matrix Invariant ◽  
Based Medicine

Objectives: The work is aimed at the theoretical substantiation and experimental development of the azimuthally invariant polarimetry method of partially depolarizing optical anisotropic biological layers on the basis of coordinate Muller-matrix mapping of histological sections for differential diagnostics of changes in optical anisotropy, which are associated with the emergence of pathological states. Results: The method of azimuthally invariant Muller-matrix mapping of optically anisotropic samples of the myocardium is proposed and grounded. The values of azimuthally invariant matrix element, superposition of matrix elements and the magnitude of the matrix vector distributions are obtained. Conclusion: The magnitude of the 1st-4th order statistical moments’ dependences, which characterize the distribution of the Muller-matrix invariant (MMI) of the histological sections of the myocardium are determined. The study of the possibility of differentiating causes of death due to ischemic heart disease (IHD) and acute coronary insufficiency (ACI) conducted from the standpoint of evidence-based medicine.

scholarly journals Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues

2019 ◽  
Vol 35 (4) ◽  
pp. 877-891 ◽  
Author(s):  
L. Trifonyuk ◽  
A. Sdobnov ◽  
W. Baranowski ◽  
V. Ushenko ◽  
O. Olar ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Mueller Matrix ◽  
Differential Diagnostics ◽  
Matrix Elements ◽  
Polycrystalline Structure ◽  
First Order ◽  
Histological Sections ◽  
Laser Polarimetry ◽  
Matrix Mapping ◽  
Differential Matrix

AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility of experimental data. This is due to azimuthal dependence of polarization and ellipticity values of most matrix elements to sample orientation in respect to incidence light polarization. Current study aims to generalize the methods of laser polarimetry for diagnosis of partially depolarizing optically anisotropic biological tissues. A method of differential Mueller matrix mapping for reconstruction of linear and circular birefringence and dichroism parameter distributions of partially depolarizing layers of biological tissues of different morphological structure is introduced and practically implemented. The coordinate distributions of the value of the first-order differential matrix elements of histological sections of brain tissue with spatially structured, optically anisotropic fibrillar network, as well as of parenchymatous tissue of the rectum wall with an “islet” polycrystalline structure are determined. Within the statistical analysis of polarization reproduced distributions of the averaged parameters of phase and amplitude anisotropy, the significant sensitivity of the statistical moments of the third and fourth orders to changes in the polycrystalline structure of partially depolarizing layers of biological tissue is observed. The differentiation of female reproductive sphere connective tissue is realized with excellent accuracy. The differential Mueller matrix mapping method for reconstruction of distributions of linear and circular birefringence and dichroism parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. Differential diagnostics of changes in the phase (good balanced accuracy) and amplitude (excellent balanced accuracy) of the anisotropy of the partially depolarizing layers of the vagina wall tissue with prolapse of the genitals is realized. The maximum diagnostic efficiency of the first-order differential matrix method was demonstrated in comparison with the traditional methods of polarization and Mueller matrix mapping of histological sections of light-scattering biological tissues.

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