scholarly journals “Intensity-Response” Effects of Electroacupuncture on Gastric Motility and Its Underlying Peripheral Neural Mechanism

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yang-Shuai Su ◽  
Wei He ◽  
Chi Wang ◽  
Hong Shi ◽  
Yu-Feng Zhao ◽  
...  
Keyword(s):  
Adult Male ◽  
Gastric Motility ◽  
Neural Mechanism ◽  
Facilitatory Effect ◽  
Response Relationship ◽  
Sprague Dawley ◽  
Trpv1 Receptor ◽  
Intensity Response ◽  
C Fiber ◽  
The Difference

The aim of this study was to explore the “intensity-response” relationship between EAS and the effect of gastric motility of rats and its underlying peripheral neural mechanism by employing ASIC3 knockout (ASIC3−/−), TRPV1 knockout (TRPV1−/−), and C57BL/6 mice. For adult male Sprague-Dawley (n=18) rats, the intensities of EAS were 0.5, 1, 3, 5, 7, and 9 mA, respectively. For mice (n=8in each group), only 1 mA was used, by which C fiber of the mice can be activated. Gastric antrum motility was measured by intrapyloric balloon. Gastric motility was facilitated by EAS at ST36 and inhibited by EAS at CV12. The half maximal facilitation intensity of EAS at ST36 was 2.1–2.3 mA, and the half maximal inhibitory intensity of EAS at CV12 was 2.8 mA. In comparison with C57BL/6 mice, the facilitatory effect of ST36 and inhibitive effect of CV12 in ASIC3−/− mice decreased, but the difference was not statistically significant (P>0.05). However, these effects in TRPV1−/− mice decreased significantly (P<0.001). The results indicated that there existed an “intensity-response” relationship between EAS and the effect of gastric motility. TRPV1 receptor was involved in the regulation of gastric motility of EAS.

scholarly journals Effects of Different Local Moxibustion-Like Stimuli at Zusanli (ST36) and Zhongwan (CV12) on Gastric Motility and Its Underlying Receptor Mechanism

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Yang-Shuai Su ◽  
Juan-Juan Xin ◽  
Zhao-Kun Yang ◽  
Wei He ◽  
Hong Shi ◽  
...  
Keyword(s):  
Gastric Motility ◽  
Crucial Role ◽  
Pain Threshold ◽  
Facilitatory Effect ◽  
Response Relationship ◽  
Wild Type ◽  
Trpv1 Receptor ◽  
Thermal Pain ◽  
Intensity Response

The aim of this study was to explore the “intensity-response” relationship in local moxibustion-like stimuli- (LMS-) modulated gastric motility and its underlying receptor mechanism. Based on the thermal pain threshold (43°C), 41°C, 43°C, and 45°C LMS were separately applied to ST36 or CV12 for 180 s among ASIC3 knockout (ASIC3−/−) mice, TRPV1 knockout (TRPV1−/−) mice, and their homologous wild-type C57BL/6 mice (n=8in each group). Gastric motility was continuously measured by an intrapyloric balloon, and the amplitude, integral, and frequency of gastric motility during LMS were compared with those of initial activities. We found that both 43°C and 45°C LMS at ST36 induced significantly facilitated effect of gastric motilityP<0.05, while LMS at CV12 induced inhibited effectsP<0.05. 41°C LMS had no significant impact on gastric motility. Compared with C57BL/6 mice, the facilitatory effect at ST36 and inhibitive effect of LMS at CV12 were decreased significantly in TRPV1−/− mice (P<0.05;P<0.01) but not changed markedly in ASIC3−/− miceP>0.05. These results suggest that there existed an “intensity-response” relationship between temperature in LMS and its effects on gastric motility. TRPV1 receptor played a crucial role in the LMS-modulated gastric motility.

Differential Effects of Variable Frequencies of Manual Acupuncture at St36 in Rats with Atropine-Induced Inhibition of Gastric Motility

2016 ◽  
Vol 34 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Li-Li Gao ◽  
Yi Guo ◽  
Tao Sha ◽  
Yang-Yang Liu ◽  
Jia-Bei Tang ◽  
...  
Keyword(s):  
Frequency Analysis ◽  
Gastric Motility ◽  
Control Model ◽  
Response Relationship ◽  
Manual Acupuncture ◽  
Sprague Dawley ◽  
Time Frequency ◽  
Sprague Dawley Rats ◽  
Intensity Response ◽  
Single Operator

Objective The ‘intensity-response’ relationship between acupuncture stimulation and therapeutic effect is currently the focus of much research interest. The same needling manipulation with different frequencies can generate differential levels of stimulus. This study aimed to examine the effects on gastric motility induced by four twirling frequencies based on relatively standardised manual acupuncture (MA) manipulations. Methods Twirling manipulations at 1, 2, 3, and 4 Hz were practised before the experiments by a single operator using an MA parameter measurement device and stability was evaluated through time-frequency analysis. Forty-eight Sprague-Dawley rats were randomly divided into six groups (n=8 each): Control, Model, Model+MA (1, 2, 3, and 4 Hz). Rats in the five Model groups received injections of atropine into the tail vein to inhibit gastric motility, which was continuously recorded by a balloon in the gastric antrum. Rats in the four Model+MA groups received MA at 1, 2, 3 and 4 Hz, respectively, for 70 s and needles were retained for a further 5 min. Results The amplitude of waveforms produced by the four twirling frequencies was relatively consistent and reproducible. The gastric motility amplitude in all groups decreased after modelling (injections of atropine) (p<0.01). Twirling manipulation at 1, 2, and 3 Hz (but not 4 Hz) increased gastric motility amplitude (p<0.05). The increase in gastric motility amplitude induced by MA at 2 Hz was greater than for all other frequencies (p<0.05). Conclusions Acupuncture at ST36 helped recover gastric motility amplitude in rats with atropine-induced gastric inhibition and the effects induced by 1–3 Hz frequency were greater than those induced by 4 Hz.

scholarly journals Electroacupuncture at ST25 Inhibits Cisapride-Induced Gastric Motility in an Intensity-Dependent Manner

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Tingting Pang ◽  
Chunxia Lu ◽  
Kaiyue Wang ◽  
Chao Liang ◽  
Zhi Yu ◽  
...  
Keyword(s):  
Gastric Emptying ◽  
Construct Validity ◽  
Therapeutic Effect ◽  
Drug Concentration ◽  
Gastric Motility ◽  
Similar Pattern ◽  
Dependent Manner ◽  
Response Relationship ◽  
Intensity Response ◽  
Relationship Of

Background.Previous studies have demonstrated the efficacy of frequency-specific EAS. However, evaluation of intensity-response effects is challenging and has yet to be addressed.Aims.Using cisapride to promote gastric emptying, we measured the intensity-response relationship of EA at ST25 on gastric motility.Methods.We determined the effects of EA at ST25 using intensities (0.5, 1, 3, 5, 7, and 9 mA) on gastric motility in rats injected with cisapride (0.2, 0.02, and 0.002 mg/kg).Results.Utilizing three concentrations of cisapride yielded significantly differing levels of gastric motility. Furthermore, log IC50values for EAS were different within each group. Given the same EA intensity, cisapride antagonism decreased progressively in each group as a function of drug concentration. The relative amount of cisapride antagonized by EAS did not change in a linear fashion. Finally, EAS at different intensities within the three groups induced a similar pattern of cisapride antagonism.Conclusions.The ability of EAS to elicit a decrease in cisapride-induced gastric motility pressure was demonstrated in this study. The study encompasses construct validity to mirror individualized treatment being based on patients’ subjective feelings, not on a set fixed EA intensity. Clinically utilizing EAS at the smallest intensity can achieve the desired therapeutic effect.

scholarly journals Electroacupuncture at ST37 Enhances Jejunal Motility via Excitation of the Parasympathetic System in Rats and Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Mengqian Yuan ◽  
Yuqin Li ◽  
Yidan Wang ◽  
Na Zhang ◽  
XuanMing Hu ◽  
...  
Keyword(s):  
Response Relationship ◽  
Wild Type ◽  
Sprague Dawley ◽  
Excitatory Effect ◽  
Parasympathetic System ◽  
Signal Collection ◽  
Sprague Dawley Rats ◽  
Intensity Response ◽  
Rats And Mice ◽  
Parasympathetic Pathway

Background.The roles of the sympathetic and parasympathetic systems in mediating the effect of electroacupuncture (EA) at ST37 on jejunal motility have yet to be demonstrated.Aim. We used rats and mice to investigate the effect and mechanism of action of EA at ST37 on jejunal motility.Methods.Jejunal motility was recorded by a balloon placed in the jejunum and connected to a biological signal collection system through a transducer. The effects of EA (3 mA) at ST37 were evaluated in Sprague-Dawley rats without drugs and with the administration of clenbuterol, propranolol, acetylcholine, and atropine. Further, the efficacy of EA at different intensities (1/2/4/6/8 mA) was measured in wild-type mice andβ1β2-/-mice andM2M3-/-mice.Results.In Sprague-Dawley rats, the excitatory effect of EA at ST37 on jejunal motility disappeared in the presence of the muscarinic receptor antagonist atropine. EA at ST37 was less effective inM2M3-/-mice than in wild-type mice. Furthermore, to a certain extent, there existed “intensity-response” relationship between jejunal motility and EA.Conclusions.EA at ST37 can enhance jejunal motility in rats and mice mainly via excitation of the parasympathetic pathway. There is an “intensity-response” relationship between EA and effect on jejunal motility.

Adrenalectomy eliminates both fiber-type differences and starvation effects on denervated muscle

1988 ◽  
Vol 255 (6) ◽  
pp. E850-E856 ◽  
Author(s):  
R. R. Almon ◽  
D. C. Dubois
Keyword(s):  
Muscle Mass ◽  
Extensor Digitorum Longus ◽  
Fiber Type ◽  
Sprague Dawley ◽  
Denervated Muscle ◽  
Adrenalectomized Animal ◽  
Sprague Dawley Rats ◽  
Starvation Effects ◽  
The Difference ◽  
Denervated Muscles

This report describes changes in muscle mass of innervated and denervated pairs of muscles taken from intact and adrenalectomized 250-g male Sprague-Dawley rats provided with different diets. Diets ranged from a nutritionally complete liquid diet to starvation (water only). In the intact animals, muscles with a more tonic character (soleus) are less sensitive to starvation than are muscles with a more phasic character (extensor digitorum longus), whereas the opposite is true of denervation. In the intact animals, starvation greatly increased the amount of atrophy following denervation. In the adrenalectomized animals, starvation had no effect on the amounts of atrophy following denervation. Furthermore, adrenalectomy virtually eliminated the fiber-type differences in the amount of atrophy following denervation. In addition, a comparison between denervated muscles from intact animals and adrenalectomized animals subjected to starvation demonstrates that all denervated muscles from the adrenalectomized animals atrophy less. Finally, it was observed that although an adrenalectomized animal can tolerate 6 days of starvation, an adrenalectomized-castrated animal cannot tolerate even short periods of starvation. The difference appears to be due to low amounts of corticosterone of testicular origin.

scholarly journals Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

2020 ◽  
Vol 19 (2) ◽  
pp. 296-303
Author(s):  
Eni Widayati ◽  
Taufiqurrachman Nasihun ◽  
Azizah Hikma Savitri ◽  
Nurina Tyagita
Keyword(s):  
Protein Expression ◽  
Caspase 3 ◽  
Medical Science ◽  
Liver Cells ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Uvb Irradiation ◽  
The Difference

Objective: The effect of Pimpinela alpina Molk (PaM) on decrease in Bax and Caspase-3 protein expression in liver cells apoptosis have been proven. However, the difference result between 7 and 15 days treatment duration of PaM need to be confirmed. This study aimed to confirm that treatment of PaM during 15 days is more effective decreasing Bax and Caspase-3 protein expression in liver cells following UVB irradiation. Methods: In the post test only control group design, 35 Sprague Dawley male rats, 300 gram body weight were divided into two arms, consisting of three groups respectively. First arm comprise Neg-7, PaM7-100, and PaM7-150. Second arm comprise Neg-15, PaM15-100, and PaM15-150. Nor-G was added as normal control neither exposed to UVB nor PaM treatment. In negative group was only radiated to UVB and PaM groups were exposed to UVB and treatment with 100, and 150 mg PaM per oral for 7 and 15 days respectively. At day 8 (first arm) and 16 (second arm), liver organ was taken and Bax and Caspase-3 protein expression assessed by Immunohistochemical staining method. Result: Post Hoc LSD analysis indicated that Bax and Caspase-3 protein expression in PaM15-100 and PaM15-150 was significant lower compared to that of Nor-G, PaM7-100, and PaM7-150, p < 0.05. Conclusion: Ttreatment of PaM with doses 100 and 150 mg for 15 days was better in decreasing Bax and Caspase-3 protein expression of liver cells following UVB irradiation. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.296-303

Hepatic effects of a highly purified PCB 180 in adult male and female Sprague–Dawley rats

2008 ◽  
Vol 180 ◽  
pp. S39-S40
Author(s):  
Robert Roos ◽  
Patrik Andersson ◽  
Päivi Heikkinen ◽  
Hans-Joachim Schmitz ◽  
Leo van der Ven ◽  
...  
Keyword(s):  
Adult Male ◽  
Sprague Dawley ◽  
Male And Female ◽  
Sprague Dawley Rats

CLINICAL TRIAL OF HUMAN GONADOTROPHINS

1966 ◽  
Vol 53 (3_Suppl) ◽  
pp. S3-S26 ◽  
Author(s):  
A. C. Crooke ◽  
W. R. Butt ◽  
P. V. Bertrand
Keyword(s):  
Clinical Trial ◽  
Dose Response ◽  
Positive Response ◽  
Test Dose ◽  
Sufficient Data ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Individual Sensitivity ◽  
The Difference ◽  
The Times

ABSTRACT Thirteen patients with prolonged secondary amenorrhoea and 5 with anovular cycles each provided sufficient data to establish their individual sensitivity to FSH. The parameters used were based on the rise in excretion of oestriol and of pregnanediol. The sensitivity expressed as the ED 50 (that dose which is effective in 50 per cent of times it is given) varied between patients over at least a sevenfold range but the difference between the 2 groups of patients was not significant. After adjusting for differences between patients a dose-response relationship was established and it was found that a dose equal to half the ED 50 would be expected to produce a response in less than Io per cent of the times it is given while a dose equal to twice the ED 50 would be expected to produce a response in more than go per cent of the times it is given. Such a dose would be likely to produce an excessive response. An excessive response occurred three times in 140 months of treatment. Sixteen of the I8 patients became pregnant. The 2 who failed gave up treatment prematurely. A test of individual patient's sensitivity to FSH was established which depended on single injections of the hormone mixed with HCG. The test was repeated at 2 to 3 weekly intervals with increasing dosages until a positive response was obtained as judged by an increase in excretion of oestriol. The rate at which the dose should be increased was investigated and a 30 per cent rate was selected. When the effective test dose was established a standard schedule of dosages was recommended for treatment using 3 equal doses of FSH in 8 days and HCG on the tenth.

Sign in / Sign up

Export Citation Format

Share Document