scholarly journals Nitric Oxide-Dependent Regulation of Cytokines Release in Type-II Diabetes Mellitus

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Maqsood M. Elahi ◽  
Bashir M. Matata
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Mononuclear Cells ◽  
Sandwich Elisa ◽  
Type Ii Diabetes Mellitus ◽  
No Production ◽  
Dependent Manner ◽  
Type Ii ◽  
Concentration Dependent Manner ◽  
Necrosis Factor

The mechanism of release of proinflammatory cytokines by blood granulocytes in diabetes is unknown. We investigated whether diabetes mellitus affects the production of cytokines by granulocytes (PMN) and mononuclear cells (PBMCs) and whether this is modulated by NO. Isolated PMN and PBMC from with or without type-II diabetes mellitus were incubated at 37°C for 6 h with S-nitroso-N-acetylpenicillamine (SNAP) at 0, 1, and 100 μM with or without lipopolysaccharides (LPS) stimulation (1 μg/mL). Supernatants were assayed for tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) by sandwich ELISA. Significant increases in TNF-α and IL-8 were observed only in PMN from diabetic subjects with or without LPS stimulation and that exogenous NO inhibited further production of cytokines in a concentration-dependent manner. However, activity of PBMC when stimulated with LPS was greatly enhanced by diabetes, but not affected by NO production. Hence, suggesting that granulocytes activation and participation in diabetes related complications is modulated by NO bioavailability.

scholarly journals Antidiabetic and Antilipidemic Activity of Root Extracts of Salacia oblonga against Streptozotocin-Induced Diabetes in Wistar Rats

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 301 ◽  
Author(s):  
Kakara Gift Kumar Deepak ◽  
Surekha Challa ◽  
Ganta Suhasin ◽  
Neelapu Nagesewara Rao Reddy ◽  
Hosam O. Elansary ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Wistar Rats ◽  
Type Ii Diabetes Mellitus ◽  
Antidiabetic Activity ◽  
Root Extract ◽  
Dependent Manner ◽  
Type Ii ◽  
Root Extracts ◽  
Salacia Oblonga

Salacia oblonga is used to treat diabetes, hypocholesteremia, gonorrhea, rheumatism, asthma, inflammation, oxidative stress, etc. In the present study, the antidiabetic activity of S. oblonga methanolic root extracts collected from three geographical locations, viz., Eleshwaram (Andhra Pradesh), Thoothukudi (Tamil Nadu), and Karwar (Karnataka), was studied in vitro. Among the three extracts, S. oblonga root extracts from Eleshwaram showed maximum α-glucosidase and α-amylase inhibitory activities, indicating better antidiabetic activity. Acute toxicity studies of S. oblonga carried out in Albino Wistar rats showed no toxicity. Further, in vivo studies showed antidiabetic and antilipidemic activities in Albino Wistar rats with induced type II diabetes mellitus. Type II diabetes mellitus was induced in the experimental rats by intraperitoneal injection of nicotinamide and streptozotocin. The rats were orally fed different doses (ranging from 0 to 400 mg/kg body weight) of S. oblonga root extracts from Eleshwaram for 14 days. Blood glucose, lipid, bilirubin, and creatinine levels were analyzed on day 0, 7, and 14. The S. oblonga root extract from Eleshwaram decreased the glucose levels in a dose-dependent manner as well as the lipid, creatinine, and bilirubin levels in diabetic rats. Thus, the present study demonstrates antidiabetic and antilipidemic properties of S. oblonga root extracts.

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