scholarly journals Correction of Aberrant NADPH Oxidase Activity in Blood-Derived Mononuclear Cells from Type II Diabetes Mellitus Patients by a Naturally Fermented Papaya Preparation

2012 ◽  
Vol 17 (3) ◽  
pp. 485-491 ◽  
Author(s):  
Ryan Dickerson ◽  
Bhakthi Deshpande ◽  
Urmila Gnyawali ◽  
Debbie Lynch ◽  
Gayle M. Gordillo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Type Ii Diabetes ◽  
Mononuclear Cells ◽  
Type Ii Diabetes Mellitus ◽  
Type Ii ◽  
Nadph Oxidase Activity

scholarly journals Nitric Oxide-Dependent Regulation of Cytokines Release in Type-II Diabetes Mellitus

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Maqsood M. Elahi ◽  
Bashir M. Matata
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Mononuclear Cells ◽  
Sandwich Elisa ◽  
Type Ii Diabetes Mellitus ◽  
No Production ◽  
Dependent Manner ◽  
Type Ii ◽  
Concentration Dependent Manner ◽  
Necrosis Factor

The mechanism of release of proinflammatory cytokines by blood granulocytes in diabetes is unknown. We investigated whether diabetes mellitus affects the production of cytokines by granulocytes (PMN) and mononuclear cells (PBMCs) and whether this is modulated by NO. Isolated PMN and PBMC from with or without type-II diabetes mellitus were incubated at 37°C for 6 h with S-nitroso-N-acetylpenicillamine (SNAP) at 0, 1, and 100 μM with or without lipopolysaccharides (LPS) stimulation (1 μg/mL). Supernatants were assayed for tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) by sandwich ELISA. Significant increases in TNF-α and IL-8 were observed only in PMN from diabetic subjects with or without LPS stimulation and that exogenous NO inhibited further production of cytokines in a concentration-dependent manner. However, activity of PBMC when stimulated with LPS was greatly enhanced by diabetes, but not affected by NO production. Hence, suggesting that granulocytes activation and participation in diabetes related complications is modulated by NO bioavailability.

Efficiency of the new metabolic operation in treatment of type II diabetes mellitus

2018 ◽  
Vol 24 (2) ◽  
pp. 16-20
Author(s):  
V.V. Grubnik ◽  
◽  
V.V. Ilyashenko ◽  
O.V. Medvedev ◽  
S.O. Usenok ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Type Ii Diabetes Mellitus ◽  
Type Ii
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