scholarly journals The Effect of Created Local Hyperosmotic Microenvironment in Microcapsule for the Growth and Metabolism of Osmotolerant YeastCandida krusei

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Guo Chen ◽  
Shanjing Yao
Keyword(s):  
Cell Growth ◽  
Osmotic Stress ◽  
Shake Flask ◽  
Culture Medium ◽  
Free Cell ◽  
Candida Krusei ◽  
Hyperosmotic Stress ◽  
Glycerol Concentration ◽  
Encapsulated Cells ◽  
Osmotolerant Yeast

Candida kruseiis osmotolerant yeast used for the production of glycerol. Addition of osmolyte such as NaCl into culture medium can increase the production of glycerol from glucose, but osmolytes may burden the glycerol separation. A coencapsulation method was suggested to create local extracellular hyperosmotic stress for glycerol accumulation. Firstly, the influence of osmotic stress induced by the addition of PEG4000 on growth and metabolism of free cell was studied in detail. Glycerol accumulation could be improved by employing PEG4000 as osmoregulator. Secondly, cells and PEG4000 were coentrapped in NaCS/PDMDAAC capsules to create local hyperosmotic stress. The effects of local hyperosmotic microenvironment on the cell growth and metabolism were studied. The coentrapment method increased the glycerol concentration by 25%, and the glycerol concentration attained 50 gL−1with productivity of 18.8 gL−1Day−1in shake flask. More importantly, the glycerol could be directly separated from the encapsulated cells. The entrapped cells containing PEG4000 were also cultivated for 15 days in an airlift reactor. The yield and productivity were ca. 35% and 21 gL−1Day−1, respectively.

scholarly journals Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture.

1982 ◽  
Vol 93 (1) ◽  
pp. 1-4 ◽  
Author(s):  
D W Barnes
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Epidermal Growth Factor ◽  
Culture Medium ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Free Cell ◽  
Bovine Insulin ◽  
A431 Cell ◽  
Epidermal Growth

A medium consisting of a rich basal nutrient mixture supplemented with bovine insulin (10 micrograms/ml), human transferrin (10 micrograms/ml), human cold-insoluble globulin (5 micrograms/ml), and ethanolamine (0.5 mM) supported the growth of the A431 human epidermoid cell line in the absence of serum with a generation time equal to that of cells in serum-containing medium. Addition of epidermal growth factor (EGF) to this culture medium at concentration mitogenic for other cell types resulted in a marked inhibition of A431 cell growth. Inhibitory effects of EGF were observed at 1 ng/ml and near-maximal effects were observed at 10 ng/ml. The inhibitory effect of EGF could be reversed by the omission of EGF in subsequent medium changes and could be prevented by the addition of anti-EGF antibody to the culture medium. Inhibition of A431 cell growth by EGF also could be demonstrated in serum-containing medium.

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

scholarly journals Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1160
Author(s):  
Atul Pradhan ◽  
Nagaraja Mukkayyan ◽  
Kishor Jakkala ◽  
Parthasarathi Ajitkumar
Keyword(s):  
Cell Growth ◽  
Growth Medium ◽  
Differential Susceptibility ◽  
Physiological Significance ◽  
Glycerol Concentration ◽  
Diadenosine Tetraphosphate ◽  
Qpcr Data ◽  
Daughter Cells ◽  
Glycerol Level ◽  
Phase Population

Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formulated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol concentration decreased to 0.1% due to cell-growth/division, the ACD proportion gradually increased until the ACD:SCD ratio reached ~50:50. With further decrease in the glycerol levels, the SCD proportion increased with concomitant decrease in the ACD proportion. Maintenance of glycerol at 0.1%, through replenishment, held the ACD:SCD proportion at ~50:50. Transfer of the cells from one culture with a specific glycerol level to the supernatant from another culture, with a different glycerol level, made the cells change the ACD:SCD proportion to that of the culture from which the supernatant was taken. RT-qPCR data showed the possibility of diadenosine tetraphosphate phosphorylase (MSMEG_2932), phosphatidylinositol synthase (MSMEG_2933), and a Nudix family hydrolase (MSMEG_2936) involved in the ACD:SCD proportion-change in response to glycerol levels. We also discussed its physiological significance.

Production of Recombinant Proteins in Chinese Hamster Ovary Cells Using A Protein-Free Cell Culture Medium

1995 ◽  
Vol 13 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Michael Zang ◽  
Helmut Trautmann ◽  
Christine Gandor ◽  
Ferruccio Messi ◽  
Fred Asselbergs ◽  
...  
Keyword(s):  
Cell Culture ◽  
Recombinant Proteins ◽  
Chinese Hamster Ovary ◽  
Culture Medium ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Ovary Cells

Effect of Different Cell Culture Medium Surfactants on Cell Growth and Viability

2010 ◽  
pp. 819-822 ◽  
Author(s):  
Kristina Martinelle ◽  
Annika Mattsson ◽  
Brita Rippner-Blomqvist ◽  
Elisabeth Lindner
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Cell Growth And Viability

Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

2007 ◽  
Vol 40 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard ◽  
Jehangir Mistry ◽  
Madhur K. Sinha
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Human Insulin ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

scholarly journals Optimization of Medium Ingredients for Keratinolytic Protease Production by Bacillus licheniformis MZK-03 using Statistical Experimental Designs

1970 ◽  
Vol 24 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mohammad Moniruzzaman ◽  
Alamgir Rahman ◽  
M Mozammel Hoq
Keyword(s):  
Trace Elements ◽  
Bacillus Licheniformis ◽  
Shake Flask ◽  
Culture Medium ◽  
Protease Production ◽  
Shake Flask Culture ◽  
Flask Culture ◽  
Statistical Approaches ◽  
Central Composite ◽  
Keratinolytic Protease

A culture medium was optimized for the production of keratinolytic protease by a newly isolated strain of Bacillus licheniformis MZK-03 in shake-flask culture. Based on the results of preliminary experiments, feather mill, molasses and trace elements were found to be major variables in keratinolytic protease production. The concentrations of these ingredients were optimized by using two statistical approaches, namely Box-Wilson method and central composite design. The optimized culture medium, finally determined by using the statistical approaches, composed of 0.95% feather mill, 0.12% molasses and 1.44% trace elements. The keratinolytic protease production was increased by approximately 2-fold when the strain was grown in the optimized medium (95.2 U/ml) compared to the un-optimized medium (56.05 U/ml). Keywords: Keratinolytic protease, Optimization, Bacillus licheniformis MZK-03, Statistical designsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1238 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 52-56

Effects Of Aspirin And Sulfinpyrazone On Platelets, Vascular Cells And Their Interactions

1981 ◽  
Author(s):  
M R Buchanan ◽  
M J Vazquez ◽  
M A Gimbrone
Keyword(s):  
Growth Rate ◽  
Cell Growth ◽  
Dna Synthesis ◽  
Vessel Wall ◽  
Culture Medium ◽  
Platelet Adhesion ◽  
Thymidine Incorporation ◽  
Inhibitory Effect ◽  
Human Platelets ◽  
Final Density

Sulfinpyrazone (SUL) and aspirin (ASA) are potentially useful antithrombotic drugs. Both drugs are thought to exert this effect by inhibiting the platelet enzyme, cyclooxygenase (C-0), thus preventing thromboxane A2 synthesis. Recent data, however, suggest that these drugs also may affect vessel wall cells. To study this further, we examined the effects of SUL and ASA on i) the adhesion of 3H-adenine-labelled washed human platelets to cultured bovine endothelial (EC) and smooth muscle cells (SMC), ii) EC and SMC DNA synthesis (3H-thymidine incorporation) and iii) cell growth. Pretreatment of platelets with 100μM ASA or 250μM SUL (concentrations sufficient to inhibit C-0), did not affect platelet adhesion to untreated EC or SMC. However, adhesion of untreated, ASA- and SUL-platelets was increased 25,28 and 44% resp. when EC were pretreated with 650μM SUL for 24 hr. In contrast, adhesion of ASA-platelets to EC pretreated with lOOμM ASA (sufficient to inhibit prostacyclin), was unaffected. Platelet adhesion to SMC pretreated with 650μM SUL for 24 hr was decreased when platelets also were pretreated with ASA (20%, p<0.05) or SUL (27%, pc 0.02). Pretreatment of SMC with SUL for only 2 hr had no effect. DNA synthesis in EC and SMC treated with 62.5 and 250μM SUL for 24 hr, was inhibited >35% and >95% resp. Preliminary data suggest that this inhibitory effect may last longer in SMC. To study the effect of SUL on cell growth, EC and SMC were plated at 2 × 104 cells/ cm2 and fed with culture medium containing 0, 62.5 or 625uM SUL on day 0, 1, 3 and 4.5. EC growth rate and final density were unaffected over 7 days. SMC growth rate also was unaffected, but the final density of SMC treated with 650μM SUL was 31 μ 2% less than untreated SMC at 7 days (p<0.01). These data indicate that SUL has direct effects on EC and SMC that may influence i) platelet-vessel wall interactions and ii) vascular cell proliferation.

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