Vascular Reactivity ConcerningOrthosiphon stamineusBenth-Mediated Antihypertensive in Aortic Rings of Spontaneously Hypertensive Rats

Orthosiphon stamineusBenth has been traditionally used to treat hypertension. The study aimed to investigate the vascular reactivity of water extract (WOS) and water : methanolic (1 : 1) extract (WMOS) ofOrthosiphon stamineusBenth and AT1receptors blocker in the mechanisms of antihypertensive mediated byα1-adrenergic receptor and EDNO and PGI2releases in the SHR aortic rings. SHR (230–280 g) were divided into four groups: control, WOS, WMOS, and losartan. After being fed orally for 14 days, the aorta was harvested and subjected to PE (10−9to 10−5 M) and ACh (10−9to 10−5 M) with and without L-NAME (100 µM) and indomethacin (10 µM), respectively. WOS, WMOS, and losartan significantly reduced the contractile responses to PE intact suggesting the importance of endothelium in vasorelaxation. Losartan significantly enhanced the ACh-induced vasorelaxation. L-NAME significantly inhibited the ACh-induced relaxation in all groups. Indomethacin enhanced ACh-induced vasorelaxation in WMOS. Collectively,Orthosiphon stamineusleaves extract reduced vasoconstriction responses by the alteration ofα1-adrenergic and AT1receptors activities. The involvement of EDNO releases was clearly observed in this plant. In WOS, PGI2releases might not participate in the ACh-induced vasorelaxation. However, in WMOS, enhancement of vasorelaxation possibly due to continuous release of PGI2.