scholarly journals Anti-Inflammatory Property of the Ethanol Extract of the Root and Rhizome ofPogostemon cablin(Blanco) Benth

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Chu-Wen Li ◽  
Xiao-Li Wu ◽  
Xiao-Ning Zhao ◽  
Zu-Qing Su ◽  
Hai-Ming Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Inflammatory Diseases ◽  
Ethanol Extract ◽  
Dependent Manner ◽  
Paw Edema ◽  
Ear Edema ◽  
Pogostemon Cablin ◽  
Mouse Ear ◽  
Anti Oxidant ◽  
Anti Inflammatory

The aim of this study was to investigate the anti-inflammatory property of the ethanol extract of the root and rhizome ofPogostemon cablin(ERP). The anti-inflammatory effect was evaluated using four animal models including xylene-induced mouse ear edema, acetic acid-induced mouse vascular permeability, carrageenan-induced mouse pleurisy, and carrageenan-induced mouse hind paw edema. Results indicated that oral administration of ERP (120, 240, and 480 mg/kg) significantly attenuated xylene-induced ear edema, decreased acetic acid-induced capillary permeability, inhibited carrageenan-induced neutrophils recruitment, and reduced carrageenan-induced paw edema, in a dose-dependent manner. Histopathologically, ERP (480 mg/kg) abated inflammatory response of the edema paw. Preliminary mechanism studies demonstrated that ERP decreased the level of MPO and MDA, increased the activities of anti-oxidant enzymes (SOD, GPx, and GRd), attenuated the productions of TNF-α, IL-1β, IL-6, PGE2and NO, and suppressed the activities of COX-2 and iNOS. This work demonstrates that ERP has considerable anti-inflammatory potential, which provided experimental evidences for the traditional application of the root and rhizome ofPogostemon cablinin inflammatory diseases.

scholarly journals Analgesic and Anti-Inflammatory Activities of the Methanol Extract fromPogostemon cablin

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Tsung-Chun Lu ◽  
Jung-Chun Liao ◽  
Tai-Hung Huang ◽  
Ying-Chih Lin ◽  
Chia-Yu Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Second Phase ◽  
Dependent Manner ◽  
Paw Edema ◽  
Pogostemon Cablin ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Factor Α

Pogostemon cablin(PC) is a herbal medicine traditionally applied to treat not only common cold, nausea and diarrhea but also headache and fever. The aim of this study was to investigate the analgesic and anti-inflammatory properties of standardized PC methanol extract (PCMeOH)in vivo. Investigations were performed in mice with two analgesic models. One was acetic acid-induced writhing response and the other formalin-induced paw licking. The anti-inflammatory effect was tested by λ-carrageenan (Carr)-induced mice paw edema. These analgesic experimental results indicated that PCMeOH (1.0 g/kg) decreased the acetic acid-induced writhing responses and PCMeOH (0.5 and 1.0 g/kg) decreased the licking time in the second phase of the formalin test. Moreover, Carr-induced paw edema inflammation was significantly reduced in a dose-dependent manner when PCMeOH (0.5 and 1.0 g/kg) was administered 3 and 4 h after the Carr injection. Mechanistic studies showed that PCMeOH decreased the levels of malondialdehyde in the edema paw by increasing the activities of anti-oxidant enzymes, such as superoxide dismutase, glutathione peroxidase and glutathione reductase, in the liver and decreasing the cyclooxygenase 2 and tumor necrosis factor-α activities in the edema paw. This study has demonstrated the analgesic and anti-inflammatory effects of PCMeOH, thus verifying its popular use in traditional medicine.

scholarly journals Analgesic, anti-inflammatory and anti-ulcer properties of Thai Perilla frutescence fruit oil in animals

2021 ◽  
Author(s):  
Narisara Paradee ◽  
Pimpisid Koonyosying ◽  
Winthana Kusirisin ◽  
Rattanaporn Janthip ◽  
Duangta Kanjanapothi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Inflammatory Diseases ◽  
Biological Activities ◽  
Water Immersion ◽  
Gastric Ulcers ◽  
Paw Edema ◽  
Ear Edema ◽  
Anti Inflammatory ◽  
Fruit Oil

Perilla frutescens fruits (PFO) is rich in α-linolenic acid (ALA) and exhibits biological activities. We aimed to investigate analgesic, anti-inflammatory and anti-ulcer activities of PFO and PFO-supplemented soybean milk (PFO-SM) in animal models. Analgesic activity was assessed in acetic acid-induced writhing in mice, while anti-inflammatory activity was performed in ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced hind paw edema in rats. Anti-ulcer effects were conducted in water immersion stress, HCl/ethanol, and indomethacin-induced gastric ulcer in rats. Distinctly, PFO (6.96 mg ALA and 2.61 LA mg equivalent/g) did not induce acute toxicity (LD50 >10 mL/kg) in mice. PFO (2.5 and 5 mL/kg) and PFO-SM (0.05 mL PFO equivalence/kg) inhibited incidences of writhing (16.8, 18 and 32.3%, respectively) in acetic acid-induced mice. In addition, topical applications of PFO (0.1 and 1 mL/ear) significantly inhibited EPP-induced ear edema (59.3 and 65.7%, respectively) in rats, while PFO-SM slightly inhibited ear edema (25.9%). However, PFO and PFO-SM did not inhibit carrageenan-induced hind paw edema in rats. Indeed, PFO (2.5 and 5 mL/kg) significantly inhibited gastric ulcers in rats that induced by water immersion stress (92.4 and 96.6%, respectively), HCl/ethanol (74.8 and 73.3%, respectively) and indomethacin (68.8 and 88.9%, respectively), while PFO-SM did not. PFO displayed potent analgesic, anti-inflammatory and anti-ulcer properties, while PFO-SM exerted only analgesic properties. Thus, Thai perilla fruit oil and its functional drink offers potential benefits in treatment of analgesic, inflammatory diseases and gastric ulcer.

Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema

2017 ◽  
Vol 45 (2) ◽  
pp. 110-117 ◽  
Author(s):  
Min-Ji Kim ◽  
Min-Ju Kim ◽  
Koth Bong Woo Ri Kim ◽  
Sun Hee Park ◽  
Hyeun Deok Choi ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Raw264.7 Cells ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Sargassum Patens ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

scholarly journals Evaluation of Analgesic and Anti-inflammatory Activities of Polyalthia simiarum (Hook. F. &Thomson)

2019 ◽  
Vol 5 (1) ◽  
pp. 18-23
Author(s):  
Selina Kabir ◽  
Ronok Zahan ◽  
Abdullah Mohammad Sarwaruddin Chowdhury ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Abdur Rashid
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Biological Activities ◽  
Dependent Manner ◽  
Paw Edema ◽  
Immersion Method ◽  
Ongoing Research ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Dose Dependent

Background: Polyalthia simiarum (Hook. F. &Thomson) exhibits different effects in human body. Objective: As a part of ongoing research on medicinal plants of Bangladesh, the present study is focused to investigate the analgesic and anti-inflammatory activities of stem bark of Polyalthia simiarum (Annonaceae). Methodology: The ethyl acetate (EA) and petroleum ether (PE) extracts were subjected to qualitative chemical investigation for the identification of different phytoconstituents. The analgesic activity was determined for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test. The anti-inflammatory activity was evaluated by carrageenan induced paw edema in rats. Analgesic and anti-inflammatory data were evaluated statistically analysed by Dunnett’s-T test. Result: Both extracts at the dose of 50- and 100 mg/kg b.w., produced significant increase in pain threshold in tail immersion method whereas significantly reduced the writhing caused by acetic acid in a dose dependent manner. The EA and PE extracts showed anti-inflammatory activities at 50- and 100 mg/kg body weight. Among all the extracts, the EA extract showed a dose dependent and comparable analgesic activity in all the tested methods and also reduced the paw edema considerably (27.5% and 39.1% inhibition after 4h), in dose dependent manner when compared to carrageenan induced control rat. Conclusion: Therefore, the EA and PE extracts of Polyalthia simiarum were capable to exhibit moderate analgesic and anti-inflammatory activities. This is the first report of analgesic and anti-inflammatory potential of Polyalthia simiarum and can be further investigated to isolate the active compounds responsible for the biological activities. Journal of National Institute of Neurosciences Bangladesh, 2019;5(1): 18-23

scholarly journals Antinociceptive and Anti-Inflammatory Effects of Total Alkaloid Extract fromFumaria capreolata

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Noureddine Bribi ◽  
Francesca Algieri ◽  
Alba Rodriguez-Nogales ◽  
Jose Garrido-Mesa ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Inflammatory Diseases ◽  
Potential Candidate ◽  
Dependent Manner ◽  
Proinflammatory Mediators ◽  
Total Alkaloids ◽  
Aerial Parts ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Fumaria Capreolata

Fumaria capreolatais used in traditional medicine in North Africa for its gastrointestinal and anti-inflammatory activities. The present study investigates the effects of total alkaloids extracted from the aerial parts ofFumaria capreolata(AFC) on LPS-induced production of proinflammatory mediators (IL-6, IL-1β, iNOS, TNF-α, COX-2, and MIP-2) in RAW264.7 cells. AFC significantly reduced the inflammatory response inhibiting the production of nitric oxide (NO) and IL-6 in a dose-dependent manner, without affecting the viability of cells, and downregulated mRNA expression of proinflammatory key players: IL-6, IL-1β, iNOS, TNF-α, and COX-2. AFC antinociceptive and anti-inflammatory properties were also evaluated on the acetic acid- and formalin-induced pain models in mice. AFC oral administration significantly inhibited acetic acid-induced writhes and reduced formalin-induced paw licking time. Therefore, AFC may be a potential candidate for the treatment of inflammatory diseases, such as colitis and arthritis.

scholarly journals Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chi-Ren Liao ◽  
Chun-Pin Kao ◽  
Wen-Huang Peng ◽  
Yuan-Shiun Chang ◽  
Shang-Chih Lai ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Paw Edema ◽  
Tissue Destruction ◽  
Hplc Fingerprint ◽  
Analgesic Effects ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Anti Inflammatory

This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the level of tissue destruction and swelling of the edema paws. This study indicated anti-inflammatory mechanism ofFPMeOHmay be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally,FPMeOHalso decreased the level of inflammatory mediators such as IL-1β, TNF-α, and COX-2. HPLC fingerprint was established and the contents of three active ingredients, rutin, luteolin, and apigenin, were quantitatively determined. This study provided evidence for the classical treatment ofFicus pumilain inflammatory diseases.

scholarly journals Effect of Pterospermum acerifolium Willd on Inflammation and ProInflammatory Cytokines

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Manna K. A ◽  
Nanda U ◽  
Kar S
Keyword(s):  
Mononuclear Cells ◽  
Dependent Manner ◽  
Paw Edema ◽  
Ear Edema ◽  
Croton Oil ◽  
Rat Paw Edema ◽  
Mouse Ear ◽  
Rat Paw ◽  
Dose Dependent ◽  
Mouse Ear Edema

In our present study, the effect of alcoholic fraction of Pterospermum acerifolium seeds on inflammation and inflammatory cytokines was evaluated in-vitro by using human peripheral blood mononuclear cells (PBMCs) and in vivo by croton oil induced mouse ear edema (acute study) and Carrageen induced rat paw edema. The extract exhibited significant inhibition of the production of tumor necrotic factor- and interleukin-6 by PBMCs stimulated lipropolysaccirides (LPS) in a dose dependent manner. The extract, at the selected dose of 150 and 300mg/kg body weight per oral exhibited significant dose dependent anti-inflammatory response with 27.77% and 47.17% inhibition of inflammation in croton oil induced mouse ear edema and Carrageen induced rat paw edema respectively.

scholarly journals Anti-Inflammatory and Analgesic Effect of Arachic Acid Ethyl Ester Isolated from Propolis

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Sélestin Dongmo Sokeng ◽  
Emmanuel Talla ◽  
Paul Sakava ◽  
Michel Archange Fokam Tagne ◽  
Celine Henoumont ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Inflammatory Diseases ◽  
Acid Ethyl Ester ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Ear Edema ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Inflammatory diseases are a real public health problem worldwide. Many synthetic drugs used in the treatment of inflammatory diseases such as steroidal anti-inflammatory drugs, nonsteroidal anti-inflammatory drugs (NSAIDs) and immunosuppressive drugs have harmful side effects. However, there are natural products like propolis, which is traditionally used in the treatment of pain. The objective of this work was to evaluate the anti-inflammatory and analgesic activities of the ethyl ester of arachic acid, a compound isolated from Cameroonian propolis. The ethyl ester of arachic acid was isolated by chromatography of the ethanolic extract of propolis harvested at Tala-Mokolo (Far North Region of Cameroon) and identified by nuclear magnetic resonance (NMR) spectra and the 1H-1H correlated spectroscopy. The anti-inflammatory and analgesic properties of oral administration of arachic acid ethyl ester (12.5, 25.0, and 50.0 mg/kg bw) were evaluated using carrageenan-induced paw edema, xylene-induced ear edema, cotton pellets-induced granuloma formation, and hot plate test in rat. Arachic acid ethyl ester produced maximum inhibition at 50.0 mg/kg for carrageenan-induced paw edema (62.5%), xylene-induced ear edema (54.5%), cotton pellet-induced granuloma (47.4%), and increased mean latency for hot plate test in rats. These results show clearly that the arachic acid ethyl ester has acute and chronic anti-inflammatory properties as well as central analgesic properties. This justifies the use of propolis in the treatment of pain in traditional medicine.

Evaluation of Antinociceptive, Anti-inflammatory and Antipyretic Effects of Strobilanthes cusia Leaf Extract in Male Mice and Rats

2003 ◽  
Vol 31 (01) ◽  
pp. 61-69 ◽  
Author(s):  
Yu-Ling Ho ◽  
Kuo-Ching Kao ◽  
Huei-Yann Tsai ◽  
Fu-Yu Chueh ◽  
Yuan-Shiun Chang
Keyword(s):  
Leaf Extract ◽  
Formalin Test ◽  
Methanol Extract ◽  
Inflammatory Diseases ◽  
Dependent Manner ◽  
Male Mice ◽  
Influenza Epidemic ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Dose Dependent

The leaf of Strobilanthes cusia (Acanthaceae), popularly known as Da-Ching-Yeh, has been commonly used in traditional Chinese medicine. It is used for influenza, epidemic cerebrospinal meningitis, encephalitis B, viral pneumonia and mumps. It is also used to treat sore throat, aphthae and inflammatory diseases with redness of skin, etc. In this study, we evaluated the antinociceptive, anti-inflammatory and antipyretic effects of methanol extract of Strobilanthes cusia leaf. The results showed that the extract significantly inhibited the writhing responses of mice and decreased the licking time on both the early and late phases of the formalin test in a dose-dependent manner. It also reduced the paw edema induced by carrageenan in rats. In addition, it potently attenuated pyrexia induced by lipopolysaccharide.

scholarly journals The Biological Evaluation of Fusidic Acid and Its Hydrogenation Derivative as Antimicrobial and Anti-inflammatory Agents

2018 ◽  
Author(s):  
Pan-Pan Wu ◽  
Hao He ◽  
W. David Hong ◽  
Tong-Rong Wu ◽  
Su-Qing Zhao ◽  
...  
Keyword(s):  
Fusidic Acid ◽  
Biological Evaluation ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Ear Edema ◽  
Gram Positive Bacteria ◽  
Mouse Ear ◽  
Anti Inflammatory

AbstractFusidic acid (WU-FA-00) is the only commercially available antimicrobial from the fusidane family that has a narrow spectrum of activity against Gram-positive bacteria. Herein, the hydrogenation derivative (WU-FA-01) of fusidic acid was prepared, and both compounds were examined against a panel of six bacterial strains. In addition, their anti-inflammation properties were evaluated using a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. The results of the antimicrobial assay revealed that both WU-FA-00 and WU-FA-01 displayed a high level of antimicrobial activity against Gram-positive strains. Moreover, killing kinetic studies were performed, and the results were in accordance with the MIC and MBC results. We also demonstrated that the topical application of WU-FA-00 and WU-FA-01 effectively decreased TPA-induced ear edema in a dose-dependent manner. This inhibitory effect was associated with the inhibition of TPA-induced up-regulation of pro-inflammation cytokines IL-1β, TNF-α and COX-2. WU-FA-01 significantly suppressed the expression levels of p65, IκB-α, and p-IκB-α in the TPA-induced mouse ear model. Overall, our results showed that WU-FA-00 and WU-FA-01 not only had effective antimicrobial activitiesin vitro, especially to the Gram-positive bacteria, but also possessed strong anti-inflammatory effectsin vivo. These results provide a scientific basis for developing fusidic acid derivatives as antimicrobial and anti-inflammatory agents.

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