scholarly journals Electroacupuncture Reduces Carrageenan- and CFA-Induced Inflammatory Pain Accompanied by Changing the Expression of Nav1.7 and Nav1.8, rather than Nav1.9, in Mice Dorsal Root Ganglia

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Chun-Ping Huang ◽  
Hsiang-Ni Chen ◽  
Hong-Lin Su ◽  
Ching-Liang Hsieh ◽  
Wei-Hsin Chen ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Inflammatory Pain ◽  
Dorsal Root ◽  
Low Frequency ◽  
Thermal Hyperalgesia ◽  
Nerve Fibers ◽  
Drg Neurons ◽  
Plantar Surface ◽  
Voltage Gated Sodium Channels

Several voltage-gated sodium channels (Navs) from nociceptive nerve fibers have been identified as important effectors in pain signaling. The objective of this study is to investigate the electroacupuncture (EA) analgesia mechanism by changing the expression of Navs in mice dorsal root ganglia (DRG). We injected carrageenan and complete Freund's adjuvant (CFA) into the mice plantar surface of the hind paw to induce inflammation and examined the antinociception effect of EA at the Zusanli (ST36) acupoint at 2 Hz low frequency. Mechanical hyperalgesia was evaluated by using electronic von Frey filaments, and thermal hyperalgesia was assessed using Hargreaves' test. Furthermore, we observed the expression and quality of Navs in DRG neurons. Our results showed that EA reduced mechanical and thermal pain in inflammatory animal model. The expression of Nav1.7 and Nav1.8 was increased after 4 days of carrageenan- and CFA-elicited inflammatory pain and further attenuated by 2 Hz EA stimulation. The attenuation cannot be observed in Nav1.9 sodium channels. We demonstrated that EA at Zusanli (ST36) acupoint at 2 Hz low-frequency stimulation attenuated inflammatory pain accompanied by decreasing the expression of Nav1.7 and 1.8, rather than Nav1.9, sodium channels in peripheral DRG neurons.

Changes in the expression of tetrodotoxin-sensitive sodium channels within dorsal root ganglia neurons in inflammatory pain

Pain ◽  
2004 ◽  
Vol 108 (3) ◽  
pp. 237-247 ◽  
Author(s):  
Joel A Black ◽  
Shujun Liu ◽  
Masaki Tanaka ◽  
Theodore R Cummins ◽  
Stephen G Waxman
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Inflammatory Pain ◽  
Dorsal Root ◽  
Dorsal Root Ganglia Neurons

scholarly journals Exon 11 skipping ofSCN10Acoding for voltage-gated sodium channels in dorsal root ganglia

Channels ◽  
2014 ◽  
Vol 8 (3) ◽  
pp. 210-215 ◽  
Author(s):  
Jana Schirmeyer ◽  
Karol Szafranski ◽  
Enrico Leipold ◽  
Christian Mawrin ◽  
Matthias Platzer ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Exon 11

Carvacrol modulates voltage-gated sodium channels kinetics in dorsal root ganglia

2015 ◽  
Vol 756 ◽  
pp. 22-29 ◽  
Author(s):  
Humberto Cavalcante Joca ◽  
Daiana Cardoso Oliveira Vieira ◽  
Aliny Perreira Vasconcelos ◽  
Demetrius Antônio Machado Araújo ◽  
Jader Santos Cruz
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels

scholarly journals Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

2021 ◽  
Vol 22 (11) ◽  
pp. 5499
Author(s):  
Veronica Corsetti ◽  
Carla Perrone-Capano ◽  
Michael Sebastian Salazar Intriago ◽  
Elisabetta Botticelli ◽  
Giancarlo Poiana ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Splice Variants ◽  
Multiple Roles ◽  
Pcr Analysis ◽  
Drg Neurons ◽  
Embryonic Developmental Stage ◽  
Embryo Stage ◽  
Dorsal Root Ganglia Neurons ◽  
Cholinergic Markers

Dorsal root ganglia (DRG) neurons synthesize acetylcholine (ACh), in addition to their peptidergic nature. They also release ACh and are cholinoceptive, as they express cholinergic receptors. During gangliogenesis, ACh plays an important role in neuronal differentiation, modulating neuritic outgrowth and neurospecific gene expression. Starting from these data, we studied the expression of choline acetyltransferase (ChAT) and vesicular ACh transporter (VAChT) expression in rat DRG neurons. ChAT and VAChT genes are arranged in a “cholinergic locus”, and several splice variants have been described. Using selective primers, we characterized splice variants of these cholinergic markers, demonstrating that rat DRGs express R1, R2, M, and N variants for ChAT and V1, V2, R1, and R2 splice variants for VAChT. Moreover, by RT-PCR analysis, we observed a progressive decrease in ChAT and VAChT transcripts from the late embryonic developmental stage (E18) to postnatal P2 and P15 and in the adult DRG. Interestingly, Western blot analyses and activity assays demonstrated that ChAT levels significantly increased during DRG ontogenesis. The modulated expression of different ChAT and VAChT splice variants during development suggests a possible differential regulation of cholinergic marker expression in sensory neurons and confirms multiple roles for ACh in DRG neurons, both in the embryo stage and postnatally.

Morphological and electrophysiological changes in mouse dorsal root ganglia after partial colonic obstruction

2005 ◽  
Vol 289 (4) ◽  
pp. G670-G678 ◽  
Author(s):  
Tian-Ying Huang ◽  
Menachem Hanani
Keyword(s):  
Glial Cells ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Neuronal Excitability ◽  
Colonic Obstruction ◽  
Resting Potential ◽  
Dye Coupling ◽  
Drg Neurons ◽  
Satellite Glial Cells ◽  
Colon Wall

There is evidence that sensitization of neurons in dorsal root ganglia (DRG) may contribute to pain induced by intestinal injury. We hypothesized that obstruction-induced pain is related to changes in DRG neurons and satellite glial cells (SGCs). In this study, partial colonic obstruction was induced by ligation. The neurons projecting to the colon were traced by an injection of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate into the colon wall. The electrophysiological properties of DRG neurons were determined using intracellular electrodes. Dye coupling was examined with an intracellular injection of Lucifer yellow (LY). Morphological changes in the colon and DRG were examined. Pain was assessed with von Frey hairs. Partial colonic obstruction caused the following changes. First, coupling between SGCs enveloping different neurons increased 18-fold when LY was injected into SGCs near neurons projecting to the colon. Second, neurons were not coupled to other neurons or SGCs. Third, the firing threshold of neurons projecting to the colon decreased by more than 40% ( P < 0.01), and the resting potential was more positive by 4–6 mV ( P < 0.05). Finally, the number of neurons displaying spontaneous spikes increased eightfold, and the number of neurons with subthreshold voltage oscillations increased over threefold. These changes are consistent with augmented neuronal excitability. The pain threshold to abdominal stimulation decreased by 70.2%. Inflammatory responses were found in the colon wall. We conclude that obstruction increased neuronal excitability, which is likely to be a major factor in the pain behavior observed. The augmented dye coupling between glial cells may contribute to the neuronal hyperexcitability.

scholarly journals Contrasting temperature-dependenee in two types of sodium channels in rat dorsal root ganglia.

1994 ◽  
Vol 64 ◽  
pp. 300
Author(s):  
Haruhiko Motomura ◽  
Fujikawa Sadatoshi ◽  
Nobukuni Ogata ◽  
Yushi Ito
Keyword(s):  
Sodium Channels ◽  
Dorsal Root Ganglia ◽  
Dorsal Root

Suppression of KCNQ/M Potassium Channel in Dorsal Root Ganglia Neurons Contributes to the Development of Osteoarthritic Pain

Pharmacology ◽  
2019 ◽  
Vol 103 (5-6) ◽  
pp. 257-262 ◽  
Author(s):  
Fan Zhang ◽  
Yani Liu ◽  
Dandan Zhang ◽  
Xizhenzi Fan ◽  
Decheng Shao ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Strong Impact ◽  
Drg Neurons ◽  
M Current ◽  
Mechanical Threshold ◽  
Dorsal Root Ganglia Neurons ◽  
M Channels ◽  
Osteoarthritic Pain

Osteoarthritic pain has a strong impact on patients’ quality of life. Understanding the pathogenic mechanisms underlying osteoarthritic pain will likely lead to the development of more effective treatments. In the present study of osteoarthritic model rats, we observed a reduction of M-current density and a remarkable decrease in the levels of KCNQ2 and KCNQ3 proteins and mRNAs in dorsal root ganglia (DRG) neurons, which were associated with hyperalgesic behaviors. The activation of KCNQ/M channels with flupirtine significantly increased the mechanical threshold and prolonged the withdrawal latency of osteoarthritic model rats at 3–14 days after model induction, and all effects of flupirtine were blocked by KCNQ/M-channel antagonist, XE-991. Together, these results indicate that suppression of KCNQ/M channels in primary DRG neurons plays a crucial role in the development of osteoarthritic pain.

Su1947 Mesotrypsin Evokes PAR2 Dependent Excitabilty of Nociceptive Dorsal Root Ganglia (DRG) Neurons

2016 ◽  
Vol 150 (4) ◽  
pp. S596 ◽  
Author(s):  
Cintya D. Lopez Lopez ◽  
Josue O. Jaramillo Polanco ◽  
Claire Rolland-Fourcade ◽  
Nathalie Vergnolle ◽  
Stephen Vanner
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Drg Neurons
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