scholarly journals Sequential Spectrophotometric Method for the Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in Coformulated Tablets

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hany W. Darwish ◽  
Said A. Hassan ◽  
Maissa Y. Salem ◽  
Badr A. El-Zeany
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Ternary Mixture ◽  
Raw Materials ◽  
Pharmaceutical Preparation ◽  
Point Theory ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Direct Spectrophotometry

A new, simple and specific spectrophotometric method was developed and validated in accordance with ICH guidelines for the simultaneous estimation of Amlodipine (AML), Valsartan (VAL), and Hydrochlorothiazide (HCT) in their ternary mixture. In this method three techniques were used, namely, direct spectrophotometry, ratio subtraction, and isoabsorptive point. Amlodipine (AML) was first determined by direct spectrophotometry and then ratio subtraction was applied to remove the AML spectrum from the mixture spectrum. Hydrochlorothiazide (HCT) could then be determined directly without interference from Valsartan (VAL) which could be determined using the isoabsorptive point theory. The calibration curve is linear over the concentration ranges of 4–32, 4–44 and 6–20 μg/mL for AML, VAL, and HCT, respectively. This method was tested by analyzing synthetic mixtures of the above drugs and was successfully applied to commercial pharmaceutical preparation of the drugs, where the standard deviation is <2 in the assay of raw materials and tablets. The method was validated according to the ICH guidelines and accuracy, precision, repeatability, and robustness were found to be within the acceptable limits.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF CEFPIROME IN PHARMACEUTICAL PREPARATION

2020 ◽  
pp. 124-127
Author(s):  
DILIP M CHAFLE
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Effect Of Temperature ◽  
Percentage Error ◽  
Ferric Nitrate ◽  
Red Color ◽  
Relative Standard

Objective: A simple, sensitive and precise visible spectrophotometric method has been proposed for the determination of cefpirome (CFM) in pure and oral injectable dosage form. Methods: A spectrophotometric method is based on the formation of stable red color product by oxidation of drugs by ferric nitrate and subsequent complexation with 1, 10 – phenanthroline with maximum absorption at 515 nm. Result: The red color complex was formed between Fe (II) and 1, 10 – phenanthroline after reduction of Fe (III) to Fe (II) in the presence of CFM drug. The phosphoric acid solution was used only for quenching the complex formation reaction. Several parameters such as the maximum wavelength of absorption, the volume of reagents, sequence of addition and effect of temperature and time of heating were optimized to achieve high sensitivity, stability and reproducible results. Under the optimum conditions, linear relationship with good correlation coefficient (0.994) was found over the concentration range from 0.20 to 6.00 μg/mL with a molar extinction coefficient 7.7813 × 104 L/mol/cm, limit of detection 0.2026 and limit of quantification 0.6141 μg/mL, respectively. Conclusion: The proposed method was evaluated statistically for linearity, accuracy, and precision in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed method can be applied for the routine estimation of CFM in the laboratory.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

scholarly journals Spectrophotometric Method for the Determination of Paracetamol

1970 ◽  
Vol 24 ◽  
pp. 39-44 ◽  
Author(s):  
Buddha Ratna Shrestha ◽  
Raja Ram Pradhananga
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Trace Amount ◽  
Pharmaceutical Preparation ◽  
Chemical Society ◽  
Molar Absorptivity ◽  
Coupling Agents ◽  
Clinical Samples ◽  
Relative Standard

Paracetamol with 1-napthol or resorcinol gave azodye and the concentration of paracetamol was investigated spectrophotometrically. The azodyes formed with both 1-napthol and resorcinol as coupling agents follow Lambert Beer's law in the range of 0 to 10 µgmL-1 of paracetamol. The molar absorptivity and Sandell's sensitivity for azodye coupled with 1-napthol were found to be 1.68×104 Lmol-1cm-1 and 9.0 ngmL-1cm-2, respectively. The molar absorptivity and Sandell's sensitivity for azodye coupled with resorcinol were found to be 2.86×104 Lmol-1cm-1 and 5.3 ngmL-1cm-2, respectively. Both coupling agents had been applied successfully in the analysis of paracetamol in pharmaceutical preparation. The relative standard deviation for all five samples ranged from 2.2-6.4% at 95% confidence. The percentage recoveries were found to range from 97.8 to 103.4. Both methods used in the present study may be applied to the determination of trace amount of paracetamol in different clinical samples. Keyword: Paracetamol; Spectrophotometric; 1-napthol; Resorcinol DOI: 10.3126/jncs.v24i0.2389 Journal of Nepal Chemical Society Vol. 24, 2009 Page: 39-44

scholarly journals DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHOTOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUINONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION

2019 ◽  
pp. 10-16
Author(s):  
MARWA K. AL JAMAL ◽  
AZZA A. GAZY
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Topical Formulation ◽  
Time Saving ◽  
Rp Hplc ◽  
Peak Areas ◽  
Development And Validation

Objective: Development and validation of spectrophotometric and RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combination in pharmaceutical preparation. Methods: The proposed spectrophotometric method was able to determine TRT directly from its absorption spectrum at 362 nm, however, HQ and HC from their first derivative spectra at 284 nm and 252 nm, respectively, without any separation step. The RP-HPLC method was developed using a C18 Sunfire© waters column with a mobile phase composed of acetonitrile: phosphate buffer (adjusted to pH 6.1 using ortho-phosphoric acid) in the ratio of 30:70 %, v/v, respectively at a flow rate of 0.8 ml/min. Quantification was based on measuring peak areas at 260 nm. Results: The spectrophotometric method was able to selectively quantify each of HQ, HC and TRT in the ranges of 10-50 µg/ml, 2-10 µg/ml and 0.5-5 µg/ml, respectively. The RP-HPLC method was able to produce well-resolved peaks after 3.0, 8.2 and 20.2 min, in the ranges of 2-10 µg/ml, 0.1-1 µg/ml and 0.05-2 µg/ml, for HQ, HC and TRT, respectively. The obtained A, D1 or peak areas values plotted against the concentration of each of the three components showed linear response in the stated ranges. Both methods were validated in terms of linearity, LOD, LOQ, precision, accuracy and selectivity. Conclusion: Both developed proposed methods were applied for the determination of the active ingredients in the pharmaceutical formulation and the common excipients did not interfere in the analysis. The RP-HPLC method proved to be more sensitive when compared to the applied spectrophotometric method. However, the applied spectrophotometric methods, considered as green analytical chemistry, is a simple, time-saving method that requires minimal use of a hazardous solvent.

scholarly journals Simultaneous Estimation and Validation of Artemether and Lumefantrine by UV Spectrophotometry in Tablet

2021 ◽  
Vol 11 (2) ◽  
pp. 16-22
Author(s):  
Megha Mishra ◽  
Anand Mundada
Keyword(s):  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Ich Guidelines ◽  
Curve Method ◽  
Tablet Dosage

A UV spectrophotometric method has been developed for the simultaneous determination of Artemether and Lumefantrine. The spectroscopic method for estimation of Artemether and Lumefantrine employed Area under curve method for analysis using Ethanol as solvent. Artemether has absorbance maxima 253.2 nm and Lumefantrine has absorbance maxima 235.2 nm and both these drugs obey Beer's law in concentration range of 4.24 -67.84 μg/ml for Artemether and 4.68 -28.08 μg/ml for Lumefantrine. The recovery studies ascertained the accuracy of the purposed method and the results were validated as per ICH guidelines. The results were found satisfactory and reproducible. The method was applied successfully for the estimation of Artemether and Lumefantrine in tablet dosage form without the interference of common excipients. Keywords: Artemether, Lumefantrine; Area under curve; Simultaneous; Estimation

scholarly journals UV Spectrophotometric Determination of Hydrochlorothiazide and Olmesartan Medoxomil in Pharmaceutical Formulation

2010 ◽  
Vol 7 (4) ◽  
pp. 1156-1161 ◽  
Author(s):  
A. T. Hemke ◽  
M. V. Bhure ◽  
K. S. Chouhan ◽  
K. R. Gupta ◽  
S. G. Wadodkar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Olmesartan Medoxomil ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Routine Work ◽  
Reproducible Method

A simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of hydrochlorothiazide and Olmesartan medoxomil in combined dosage form by UV spectrophotometric method. UV spectrophotometric method includes Simultaneous equation method (Method I) 271.5 nm and 257.0 nm λmaxof both the drugs were selected, absorbance Ratio method (Method II) 261.5 nm an isoabsorptive wavelength and 257.0 nm were selected for estimation of hydrochlorothiazide and Olmesartan medoxomil respectively and Three-wavelength method (Method III), two wavelengths were selected such that hydrochlorothiazide give same absorbances (263.8 and 278.4 nm) at two selected wavelength while third wavelength (316.5 nm) was such that olmesartan gives nearly zero absorbance. The two drugs follow Beer’s law over the concentration range of 5-25 μg/mL. The % recoveries of the both the drugs were found to be nearly 100 % representing the accuracy of the proposed methods. Validation of the proposed methods was carried out for its accuracy, precision, specificity and ruggedness according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of hydrochlorothiazide and olmesartan medoximil in combined dosage form.

APPLICATION OF RATIO DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ARTEMISININ AND CURCUMIN

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 43-47
Author(s):  
R Dhankecha ◽  
◽  
A Soni ◽  
M Gohel ◽  
V Thakkar ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Absorption Spectra ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Direct Absorption ◽  
Acceptable Limit ◽  
First Derivative ◽  
Ich Guidelines

Ratio derivative spectrophotometric method has been developed for the simultaneous determination of artemisinin and curcumin. The overlapping spectra of both drugs were resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of artemisinin and curcumin were measured at λmax 293.17 and λmax 457.0 nm, respectively, for their quantification. Artemisinin and curcumin were determined in the concentration range of 2-10 μg/mL and 1-5 μg/mL, respectively. The method was validated as per the ICH guidelines and accuracy, precision were found to be within acceptable limit. The limits of detection and quantitation were found to be 0.003299 and 0.009997 μg/mL, respectively for artemisinin and 0.006743 and 0.020434 μg/mL, respectively for curcumin. The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and successfully applicable for simultaneous estimation of artemisinin and curcumin in parentral dosage form.

To Estimate the Label claim of tablet in their combination by simultaneous estimation using UV-Visible Spectrophotometric method

2021 ◽  
pp. 191-194
Author(s):  
Yelekar P D ◽  
Chaudhari S.B ◽  
Chourasia R D ◽  
Tikariya K R ◽  
Badole Payal
Keyword(s):  
Spectrophotometric Method ◽  
Method Development ◽  
Tablet Formulation ◽  
Simultaneous Estimation ◽  
Distilled Water ◽  
Ich Guidelines ◽  
Water As Solvent ◽  
Label Claim ◽  
Development And Validation

Objective: The main objective of the work was to check the label claim of tablet in combination by Simultaneous estimation by UV method. Method: Spectrophotometric method development and validation are plays important role in the development and manufacture of pharmaceuticals. This Spectrophotometric method was a simple and reproducible for the quantitative determination of Paracetamol and Caffeine in tablet formulation was developed and validated in the present work. The various parameters like specificity, linearity, precision, accuracy, robustness and ruggedness were studied according to ICH guidelines. The wavelength 273nm was selected for the estimation of Caffeine using distilled water as a solvent and the wavelength 243nm selected for the estimation of Paracetamol using distilled water as solvent the drug obeyed Beer’s-Lambert’s law over the concentration range 20-120µg/ml. Recovery study was performed to confirm the accuracy of the method. The method was successfully applied for routine analysis of this drug in formulation the method were validated as pr ICH guidelines. Conclusion: A simple UV spectrophotometric method was developed for the Simultaneous determination of Paracetamol and Caffeine in tablet formulation without any interference from the excipients. The present method succeeded in adopting a simple sample preparation that achieve satisfactory extraction recovery and facilitated its application in co formulated formulation.

scholarly journals Estimation of Furosemide in Pharmaceutical Preparation Samples

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Oral Solution ◽  
Average Recovery ◽  
Relative Standard

A simple, accurate, precise, rapid, economical and sensitive ultraviolet spectrophotometric method has been developed for the determination of Furosemide in pharmaceutical preparations, which shows maximum absorbance at 228 nm in. Beer’s law was obeyed in the range of 1 -10 μg/ ml, with molar absorptivity of 3.5×104 L.mol-1.cm-1, relative standard deviation of the method was less than 1.4%, and accuracy (average recovery %) was 100 ± 1.0. The method was successfully applied to the determination of Furosemide in some pharmaceutical formulations (tablets, Oral solution and injection) samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Furosemide in true samples.

scholarly journals Simultaneous UV Spectrophotometric Determination of Cetrizine and Dextromethorphan in Tablet Dosage Form

2010 ◽  
Vol 7 (s1) ◽  
pp. S314-S318 ◽  
Author(s):  
R. Vijayalakshmi ◽  
S. Bhargavi ◽  
M. D. Dhanaraju
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Determination ◽  
Dosage Form ◽  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Ich Guidelines ◽  
Tablet Dosage

Two accurate, precise, sensitve and economical procedures for simultaneous estimation of cetrizine and dextromethorphan in tablet dosage forms have been developed. First method employs formation and solving of simultaneous equations using 230 nm and 280 nm as two analytical wavelengths for both drugs in methanol. The second method isQ-analysis based on measurement of absorptivity at 224 nm (as isobestic point) and 280 nm (λmaxof CTZ). Cetrizine and dextromethorphan at their respective λmax280 nm and 230 nm and at 224 nm (isobestic point) shows linearity in a concentration range of 10-30 mcg/mL for both the drugs. The recovery studies confirmed accuracy of the proposed methods and low values of standard deviation confirmed precision of the methods. The methods were validated as per ICH guidelines.

Sign in / Sign up

Export Citation Format

Share Document