scholarly journals Prostaglandin Analogous and Antioxidant Activity Mediated Gastroprotective Action ofTabernaemontana divaricata(L.) R. Br. Flower Methanolic Extract against Chemically Induced Gastric Ulcers in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Mohammed Safwan Ali Khan ◽  
Abdul Manan Mat Jais ◽  
Adiba Afreen
Keyword(s):  
Superoxide Dismutase ◽  
Total Protein ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Basic Mechanism ◽  
Gastric Ulcers ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Mass Spectral ◽  
Antioxidant Mechanism

The present study was conducted to evaluate the antiulcerogenic effect and recognize the basic mechanism of action ofTabernaemontana divaricata(L.) R. Br. flowers.T. divaricataflower methanolic extract (TDFME) was screened for antiulcer activity versus aspirin and ethanol induced gastric ulcers at three doses—125, 250, and 500 mg/kg—orally using misoprostol as a standard. Besides histopathological examination, seven parameters, that is, ulcer index, total protein, nonprotein sulphhydryls, mucin, catalase, malondialdehyde, and superoxide dismutase levels, were estimated. In addition to HPLC profiling, GC-MS analysis and electrospray ionization—high resolution mass spectral (ESI-HRMS) analysis of crude TDFME were carried out in an attempt to identify known phytochemicals present in the extract on the basis ofm/zvalue. The results revealed a significant increase in the levels of catalase, superoxide dismutase, mucin, and nonprotein sulphhydryls, while they revealed a reduction in ulcer index, the levels of total protein, and malondialdehyde. Histopathological observations also demonstrated the protective effect. Though all the doses of TDFME exhibited gastroprotective function, higher doses were found to be more effective. Mass spectral analysis gave a few characteristicm/zvalues suggesting the presence of a few known indole alkaloids, while HPLC profiling highlighted the complexity of the extract. TDFME was found to exhibit its gastroprotective effect through antioxidant mechanism and by enhancing the production of gastric mucous.

scholarly journals FLAVONOIDS AND ANTI-OXIDANT ACTIVITY MEDIATED GASTROPROTECTIVE ACTION OF LEATHERY MURDAH, TERMINALIA CORIACEA (ROXB.) WIGHT & ARN. LEAF METHANOLIC EXTRACT IN RATS

2017 ◽  
Vol 54 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Mohammed Safwan ALI KHAN ◽  
Shaaz NAZAN ◽  
Abdul Manan MAT JAIS
Keyword(s):  
Superoxide Dismutase ◽  
High Performance ◽  
Methanolic Extract ◽  
Ultra Violet ◽  
High Performance Liquid Chromatographic ◽  
Ulcer Index ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Anti Oxidant ◽  
Liquid Chromatographic

ABSTRACT BACKGROUND Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. from family Combretaceae is used in Ayurveda and Siddha traditional systems of medicine to heal ulcers. OBJECTIVE The present study was conducted to assess the gastroprotective effect and understand the fundamental mechanism of action of Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. Leaf Methanolic Extract. METHODS The test extract was screened for anti-ulcer activity by Aspirin induced ulcerogenesis in pyloric ligation and ethanol induced gastric ulcers at three doses - 125, 250, and 500 mg/kg, p.o. using Ranitidine 50 mg/kg and Misoprostol 100 μg/kg as standard drug in respective models. Seven parameters were carefully examined, that is, ulcer index, total protein, mucin, catalase, malondialdehyde, and superoxide dismutase levels and histopathology. High Performance Liquid Chromatographic - Ultra Violet profiling and Liquid Chromatography - Mass Spectral analysis of crude Terminalia coriacea leaves methanolic extract were carried out as a part of chemical characterization to identify bioactive compounds. RESULTS All the test doses exhibited significant gastroprotective function, particularly the higher doses demonstrated improved action. The results revealed a significant increase in the levels of catalase, superoxide dismutase, and Mucin with reduction in ulcer index, the levels of total protein, and malondialdehyde. Histopathological observations also illustrated the gastroprotective effect of Terminalia coriacea leaves methanolic extract. CONCLUSION Terminalia coriacea leaves methanolic extract exhibited strong anti-oxidant and anti-secretory activities mediated gastroprotection besides inducing the gastric mucosal production. The observed pharmacological response can be attributed to the flavonoidal compounds namely - Quercetin-3-O-rutinoside, Luteolin-7-O-glucoside, Myricetin hexoside, Quercetin-3-O-glucoside, Isorhamnetin-3-O-rhamnosylglucoside and Isorhamnetin-3-O-glucoside identified in the extract for the first time with High Performance Liquid Chromatographic - Ultra Violet and Liquid Chromatography - Mass Spectral analysis.

Evaluation of the Antiulcer Activity of Ethanolic Leave Extracts of Saraca indica against Ethanol, Pylorus Ligature and Indomethacin Induced Ulcer in Albino Rats

2020 ◽  
Vol 16 (8) ◽  
pp. 1191-1196
Author(s):  
Pritt Verma ◽  
Shravan K. Paswan ◽  
Vishal K. Vishwakarma ◽  
Priyanshi Saxena ◽  
Chandana V. Rao ◽  
...  
Keyword(s):  
Mucosal Injury ◽  
Gastric Volume ◽  
Gastric Ulcers ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Cellulose Solution ◽  
Total Acidity ◽  
Ulcer Index ◽  
Standard Drug ◽  
High Doses

Background: To evaluate the antiulcer activity of ethanolic leaves extract of Saraca indica against ethanol, pylorus ligature and indomethacin in albino rats. Materials and Methods: Ulcer was produced by ethanol, pylorus ligature and indomethacin in albino rats. Five groups (n=6) of rats were orally pre-treated with carboxymethyl cellulose solution, and ranitidine (80 mg/kg) respectively. In ethanol induced ulcer, the animals were treated with 200 and 400 mg/kg b.w. ethanolic leave extracts of Saraca indica in 0.3% CMC solution, 60 minutes before oral administration of absolute ethanol to produce gastric mucosal injury. In indomethacin induced ulcer, the drug was administered orally at the dose of 30 mg/kg b.w. After 7-9 hours of administration of indomethacin (30 mg/kg); the animals were sacrificed with high doses of anesthesia. In the pylorus ligature method, volume of free acidity, gastric secretion, pH and total acidity were estimated. In all three models, the ulcer index and % protection were estimated. Results: The anti ulcer activity of ethanolic leave extracts of Saraca indica in ethanol, indomethacin and pylorus ligature models is evident from the significant (P<0.001) reduction in ulcer index. In pylorus ligature model, significant (P<0.001) reduction in total acidity gastric volume and increase in pH were observed when compared with the standard drug. Conclusion: Ethanolic leave extracts of Saraca indica were found to be significantly protective against ethanol, indomethacin and pylorus ligature induced gastric ulcers in the experimental albino rats. The result obtained suggest that ethanolic leave extracts of Saraca indica possesses significant anti-ulcer activity.

Antiulcer activity of Lagerstroemia indica leaves in Indomethacin Induced Gastric Ulcer in Rats

2021 ◽  
pp. 4408-4412
Author(s):  
Sweety Saini ◽  
Chandana Majee
Keyword(s):  
Methanolic Extract ◽  
Antiulcer Activity ◽  
Standard Group ◽  
Ulcer Index ◽  
Traditional Use ◽  
Ancient Medicine ◽  
Model Method ◽  
Male Wistar Rats ◽  
Ulcer Model ◽  
Histopathological Investigation

Lagerstroemia indica, is a natural plant which have traditional use from the ancient medicine for the treatment of many diseases. The gastroprotective effect of methanolic extract of Lagerstroemia indica and the possible mechanisms used to investigated the ulcer activity through indomethacin-induced ulcer model. Method: male Wistar rats were divided into three groups: (normal control, n=6), (standard omeprazole group, n=6) and (extract group, n=6). Each of control, omeprazole and extract were administered orally to the rats daily for 21 days before the induction of ulcer with the help of indomethacin. On the 21 days indomethacin was administered with single oral dose (20mg/kg). Four hours later after the administration of indomethacin dose treatment, the rats were sacrificed and gastric tissue was taken for histopathological investigation for the calculation of pH, ulcer inhibition, ulcer index. The observation significantly increased (p < 0.01) value that reduced the ulcer by the treatment with Lagerstroemia indica. Result: The result indicates the antiulcer effect of the Lagerstroemia indica extracts showed significant effect during comparision with control and standard group. The data of study revealed that the extracts of Lagerstroemia indica proved to show control over the model of indomethacin-induced gastric ulceration which gives potent activity against ulceration.

scholarly journals Exploration of Hepatoprotective Potential and Phytochemicals of Ziziphus oxyphylla Edgew

2020 ◽  
Vol 40 (04) ◽  
pp. 431-436
Author(s):  
Abdullah .
Keyword(s):  
Superoxide Dismutase ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Liver Weight ◽  
2D Nmr ◽  
Chloroform Fraction ◽  
Hypotonic Solution ◽  
2D Nmr Spectroscopy ◽  
Hepatic Diseases ◽  
Antihepatotoxic Activity

Ziziphus oxyphylla Edgew (ZO) has been traditionally used for the treatment of hepatic diseases in Pakistan. The current study was carried out to investigate the effectiveness and mechanism underlying hepatoprotective effect of ZO. Hepatoprotective potential of root extracts (crude methanolic extract and subsequent fractions) was investigated against carbon tetrachloride (CCl4) induced hepatotoxicity in BALB/c mice via determination of serum biomarkers, antioxidant enzymes (catalase, superoxide dismutase), lipid peroxidation and histopathology. Membrane stabilizing activity was determined via inhibition of hypotonic solution and heat-induced hemolysis. Crude methanolic extract and chloroform fraction ameliorated CCl4 induced elevated levels of alanine transaminase (ALT), alkaline phosphatase (ALP), total proteins (TP), total bilirubin (TB) and reversed the antioxidants imbalance in terms of catalase (CAT), superoxide dismutase (SOD) and reduced malondialdehyde (MDA) level. Crude methanol extract inhibited hemolysis induced by hypotonic solution and heat. Histopathological examination of liver specimens of extracts administered mice exhibited recovery from CCl4 induced pathological changes. Moreover, CCl4 induced alteration in body and liver weight was countered by the administration of ZO. Compounds betulinic acid (AZO1), ceanothic acid (AZO2) and zizybrenalic acid (AZO3) were isolated from chloroform fraction of the plant. The structures of compounds were deduced using 1D-and 2D-NMR spectroscopy techniques. The results show that Ziziphus oxyphylla roots extract possess antihepatotoxic activity mediated by the protection of anti-oxidant defense system and membrane-stabilizing activity, probably due to the presence of pentacyclic triterpenes

scholarly journals Gastroprotective Effect of Freeze Dried Stripped Snakehead Fish (Channa striata Bloch.) Aqueous Extract against Aspirin Induced Ulcerogenesis in Pylorus Ligated Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mohammed Safwan Ali Khan ◽  
Abdul Manan Mat Jais ◽  
Javeed Hussain ◽  
Faiza Siddiqua ◽  
A. Gopala Reddy ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Gastric Juice ◽  
Aqueous Extract ◽  
Antiulcer Activity ◽  
Fresh Water Fish ◽  
Ulcer Index ◽  
Standard Drug ◽  
Freeze Dried ◽  
Wide Range ◽  
Channa Striata

Channa striata (Bloch.) is a fresh water fish belonging to the family Channidae. The stripped snakehead fish possesses wide range of medicinal properties. In view of traditional use of C. striata for wound healing, the present study was undertaken to investigate the beneficial effects of orally administered freeze dried aqueous extract of Channa striata (AECS) in experimentally induced gastric ulcers in Wistar rats. Aspirin induced ulcerogenesis in pyloric ligation model was used for the assessment of antiulcer activity and Ranitidine (50 mg/kg) was employed as the standard drug. The various gastric parameters like volume of gastric juice, pH, free and total acidities, ulcer index, and levels of antioxidant enzymes like catalase, superoxide dismutase, and lipid peroxidation marker malondialdehyde were determined. AECS at concentrations of 40% and 50% w/v significantly decreased the volume of gastric juice and increased the levels of catalase while considerable decrease in free and total acidities and increase in superoxide dismutase were observed with the treatment of standard drug and AECS (50% w/v). All the test doses of AECS markedly decreased ulcer index and malondialdehyde compared to the standard drug whereas AECS 30% w/v did not alter volume of gastric juice, pH, free and total acidities, catalase, and superoxide dismutase. From these findings, it can be concluded that AECS is devoid of acid neutralizing effects at lower doses and possesses antisecretory and antiulcer activities and this could be related to its antioxidant mechanism.

scholarly journals Gastroprotective Effect of Symplocos Racemosa Whole Plant Methanolic Extract Against Experimentally Induced Gastric Ulcer in Rat

2019 ◽  
Vol 7 (2) ◽  
pp. 59-61
Author(s):  
R. K. Gupta ◽  
Ravi Vishwakarma ◽  
Yashwant Giri ◽  
Varinder Singh
Keyword(s):  
Gastric Ulcer ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Gastric Volume ◽  
Gastric Ulceration ◽  
Antiulcer Activity ◽  
Free Acidity ◽  
Ulcer Index ◽  
Whole Plant ◽  
Symplocos Racemosa

Peptic ulcer is a condition which results from an imbalance between offensive and defensive factors of gastrointestinal system. The investigation was designed to evaluate the antiulcer activity of Symplocos racemosa whole plant methanol extract (MESR) in rat model of indomethacininduced gastric ulceration. The total acidity, gastric volume, pH and free acidity were measured to determine the anti-ulcer activity of MESR. Pretreatment with MESR (125-500 mg/kg) markedly reduced the indomethacin-induced increase in gastric ulcer index and score. These results revealed that antisecretory effects MESR were responsible for antiulcer activity of MESR.

Gastroprotective activity of essential oils from turmeric and ginger

2015 ◽  
Vol 26 (1) ◽  
Author(s):  
Vijayasteltar B. Liju ◽  
Kottarapat Jeena ◽  
Ramadasan Kuttan
Keyword(s):  
Gastric Ulcer ◽  
Antioxidant Enzymes ◽  
Essential Oils ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Degree Of Protection ◽  
Male Wistar Rats ◽  
Different Doses

AbstractTurmeric (Turmeric and ginger were evaluated for their antiulcer activity against ethanol-induced ulcers in male Wistar rats at different doses: 100, 500 and 1000 mg/kg body weight. Ethanol was used to induce gastric ulcer in Wistar rats. Parameters such as ulcer index, histopathology and levels of antioxidant enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase and glutathione (GSH) levels were measured to assess the degree of protection produced by the essential oils.TEO and GEO inhibited ulcer by 84.7% and 85.1%, respectively, as seen from the ulcer index. Reduced antioxidant enzymes such as GPx, SOD, catalase and GSH produced by alcohol administration were significantly (p<0.001) increased by simultaneous administration of TEO and GEO. Histopathological examination showed that ethanol-induced lesions such as necrosis, erosion and hemorrhage of the stomach wall were significantly reduced after oral administration of essential oils.Results suggest that TEO and GEO could reduce the gastric ulcer in rat stomach as seen from the ulcer index and histopathology of the stomach. Moreover, oxidative stress produced by ethanol was found to be significantly reduced by TEO and GEO.

scholarly journals Antiulcer Activity of Morinda Citrifolia Linn Fruit Extract

2009 ◽  
Vol 1 (2) ◽  
pp. 345-352 ◽  
Author(s):  
J. Srikanth ◽  
P. Muralidharan
Keyword(s):  
Oral Administration ◽  
Free Acid ◽  
Test Group ◽  
Treated Group ◽  
Morinda Citrifolia ◽  
Gastric Ulcers ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Total Acid ◽  
Standard Drug

The study was designed to investigate the antiulcer activity of ethyl acetate extract of the fruits of Morinda citrifolia Linn (Rubiaceae) using different models of gastric and duodenal ulceration in rats. Gastric ulcers were induced by oral administration of ethanol, aspirin and by pyloric ligation and duodenal ulcers were induced by oral administration of cysteamine HCl. The extract was administered at a dose of 200 and 400 mg/kg orally 30 min prior to ulcer induction. Ranitidine (50 mg/kg) was used as a reference standard.  The antiulcer activity was accessed by determining and comparing the ulcer index in the test group with that of the standard drug treated group. Gastric volume, total acid and free acid were estimated in the pylorus-ligated rats. M.citrifolia (400mg/kg) showed maximum inhibition of gastric acid, free acid and total acid to 53.54%, 52.55% and 30.30%, respectively. The ulcer index in the M.citrifolia treated animals was found to be significantly less in all the models compared to standard drug treated cases. The antiulcer activity of M.citrifolia was, however, less than that of ranitidine. The results suggest that M.citrifolia possesses significant antiulcer property which could be due to cytoprotective action of the drug or strengthening of gastric and duodenal mucosa with the enhancement of mucosal defence. Keywords: Morinda citrifolia; Antiulcer.© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1625

scholarly journals Comparative evaluation of antiulcer activity of combination of extracts of Embilica officinalis Gaertn and Musa sapientum Linn in rats

2019 ◽  
Vol 10 (2) ◽  
pp. 1535-1539
Author(s):  
Keserla Bhavani ◽  
Gnanavel V ◽  
Ratna B ◽  
Jayakar B ◽  
Aanandan ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Methanolic Extract ◽  
Gastric Volume ◽  
Antiulcer Activity ◽  
Total Acidity ◽  
Emblica Officinalis ◽  
Free Acidity ◽  
Ulcer Index ◽  
Musa Sapientum ◽  
Unripe Fruits

The present study is to evaluate the anti-ulcer activity of methanolic extract of unripe fruits of Musa Sapientum Linn. and the fresh juice of the fruits of Emblica Officinalis Gaertn in Wistar rats. The methanolic extract unripened fruits of Musa Sapientum Linn.  and the fresh juice of the fruits of Emblica Officinalis Gaertn were combined and evaluated for the gastroprotective activity by ethanol-induced model and indomethacin-induced model in rats at a dose of 25mg/kg (p.o) and 50mg/kg (p.o) respectively. Biochemical parameters like ulcer index, the volume of gastric juice, pH of gastric juice, free acidity and total acidity were studied. The unripe fruits of Musa and the fresh juice of Embilica will contain flavonoids, flavones, abscisic acid which shows a significant reduction in gastric volume, pH, free acidity, total acidity and ulcer index at the doses of 25mg/kg (p.o) and 50mg/kg (p.o). The results show that the unripe fruits 0f Musa Sapientum Linn. and fresh juice from the fruits of Emblica Officinalis Gaertn will combinedly possess greater antiulcer activity than that of the individual drugs.

scholarly journals Pharmacological and Phytochemical Evaluation Of Gastric Anti-Ulcer Activity of Parthenium hysterophorus In Different Models

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Ananda Kumar Chettupalli ◽  
Vasudha B. ◽  
Krishna Sanka
Keyword(s):  
Gastric Ulcers ◽  
Gastrointestinal Disorders ◽  
Antiulcer Activity ◽  
Peptic Ulcers ◽  
Gastric Mucus ◽  
Ulcer Index ◽  
Traditional System ◽  
Ulcer Model ◽  
Dose Dependent ◽  
Lesion Index

The cause of ulceration in patients is mainly due to hyper secretion of gastric juice and also due to hyper secretion of pepsin. In traditional system of medicine a number of herbal preparations have been used for the treatment of peptic ulcers. There are various medicinal plants has been used for the treatment of gastrointestinal disorders. In view of this, in present study we have to evaluate the anti-ulcer activity of parathenium hysterophorus. Study was carried out, by using three methods i.e., alcohol, paracetamol and stress induced ulcers in rats pretreated with the doses of 250 mg/kg AQPH and ALPH, 20mg/kg Omeoprazole and 50 mg/kg Ranitidine. To evaluate the antiulcer activity of aqueous and alcoholic extracts of parathenium hysterophorus leaves (AQPH and ALPH) at 250 doses using different experimentally induced gastric ulcer models in rats. Gastric ulcers were induced in rats by 80% alcohol, paracetamol and forced immersion stress induced methods. In alcohol induced ulcer model, paracetamol induced ulcer model and stress induced model the ulcer index was determined. Where as in stress induced ulcers stress plays an important role in ulcerogenesis. In alcohol-induced ulcers, AQPH and ALPH were effective in reducing lesion index and increasing the gastric mucus content. It was also effective in decreasing ulcer index in paracetamol-induced ulcers. All the results obtained with parathenium hysterophorus were dose dependent. The results suggest that AQPH and ALPH possesses significant and dose dependent antiulcer activity. The antiulcer activity of AQPH and ALPH can be attributed to its cytoprotective and antisecretory action.

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