scholarly journals Inhibitory Effect of Aqueous Extract of Stem Bark of Cissus populnea on Ferrous Sulphate- and Sodium Nitroprusside-Induced Oxidative Stress in Rat’s Testes In Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Seun F. Akomolafe ◽  
Ganiyu Oboh ◽  
Afolabi A. Akindahunsi ◽  
Ayodele J. Akinyemi ◽  
Oluwatosin G. Tade
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Sodium Nitroprusside ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Tissue Homogenate ◽  
Dependent Manner

Cissus populnea are plants associated with a myriad of medicinal uses in different parts of the world and are good sources of carotenoids, triterpenoids, and ascorbic acid. The antioxidant properties and inhibitory effect of water extractible phytochemicals from stem bark of C. populnea on FeSO4 and sodium nitroprusside- (SNP-) induced lipid peroxidation in rat testes were investigated in vitro. The results revealed that the extract was able to scavenge DPPH radical, chelate Fe2+ and also had a high reducing power. Furthermore, the incubation of the testes tissue homogenate in the presence of FeSO4 and SNP, respectively, caused a significant increase in the malondialdehyde (MDA) contents of the testes. However, the aqueous extract of the stem bark of C. populnea caused a significant decrease in the MDA contents of both Fe2+ (EC50 = 0.027 mg/mL) and SNP- (EC50 = 0.22 mg/mL) induced lipid peroxidation in the rat testes homogenates in a dose-dependent manner. The water extractible phytochemicals from C. populnea protect the testes from oxidative stress and this could be attributed to their high antioxidant activity: DPPH-scavenging ability, Fe2+-chelating and -reducing power. Therefore, oxidatively stress in testes could be potentially managed/prevented by this plant.

scholarly journals Antioxidant properties of Ageratum conyzoides L. Asteraceae leaves

2018 ◽  
Vol 53 (4) ◽  
pp. 265-276 ◽  
Author(s):  
FO Adetuyi ◽  
KO Karigidi ◽  
ES Akintimehin ◽  
ON Adeyemo
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Erectile Dysfunction ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Tissue Homogenate ◽  
Total Phenolic ◽  
Ageratum Conyzoides

In vitro antioxidative and anti-lipid peroxidative properties of aqueous and methanol extracts of Ageratum conyzoides leaves were studied in controlling erectile dysfunction caused by oxidative stress. Methanol extract gave a significantly (P ˂ 0.05) higher content of total phenolic (61.4 mgGAE/g), total flavonoid (42.2 mgQE/g), ascorbic (10.1 mgAAE/100g) and phosphomolybdate (45.8 mgAAE/g) than the aqueous extract. The result showed that the extracts have high antioxidant activities. However, the methanol extract showed a higher DPPH and hydrogen peroxide scavenging activities over aqueous extract but the aqueous extract had a higher reducing power. The methanol extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ in rat pancreas and penile tissue homogenate exemplified by their least IC50 (94.21 μg/ml in pancreas) and (75.95 μg/mL in penile tissue) while in rat brain homogenate the aqueous extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ with least IC50 of 91.74 μg/mL. Hence, these extracts can be used as a potent natural antioxidant against free radicals and as a natural source of combating erectile dysfunction caused by oxidative stress. The extracts of Ageratum conyzoides leaves could be useful therapeutically as erectogenic agent.Bangladesh J. Sci. Ind. Res.53(4), 265-276, 2018

scholarly journals Protective role of Limonium bonduelli extract against non-enzymatic peroxidation in brain and testes induced by iron in vitro.

2017 ◽  
Vol 9 (1) ◽  
pp. 72
Author(s):  
Amel Amrani ◽  
Nassima Boubekri ◽  
Ouahiba Benaissa ◽  
Djamila Zama ◽  
Fadila Benayache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Inhibitory Effect ◽  
Protective Role ◽  
Dependent Manner ◽  
Phytochemical Content ◽  
Butanol Extract ◽  
Aerial Parts

<p>Infertility and Neurodegerative diseases have been linked to oxidative stress arising from peroxidation of membrane biomolecules and high levels of iron have been reported to play an important role. The present study sought to determine the antioxidant activity and protective ability of <em>n</em>-butanol extract of <em>Limonium bonduelli</em> on lipid peroxidation induced by FeSO<sub>4</sub> in rat brain and testes homogenates <em>in vitro</em>. <em>n</em>-butanol extract of the aerial parts (leaves and flowers)<em> </em>was prepared, and the ability of the extract to inhibit FeSO<sub>4</sub> induced lipid peroxidation in isolated rat brain and testes was determined using spectrophotometric method. The study revealed that the extract inhibited malondialdehyde (MDA) production in FeSO<sub>4 </sub>induced lipid peroxidation in the brain and testes in a dose-dependent manner and the highest percentage of the inhibition was 89.80% similar to vitamin C in the same concentration (100 µg/mL) in brain and 82.33% in testes (200 µg/mL). <em>Limonium bonduelli</em> extract<strong> </strong>demonstrated important anti-lipid peroxidative effect, which may be useful in preventing the progress of various oxidative stress related diseases. The higher inhibitory effect of the extract could be attributed to its phytochemical content.</p>

scholarly journals An Antioxidant Phytotherapy to Rescue Neuronal Oxidative Stress

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Zhihong Lin ◽  
Danni Zhu ◽  
Yongqing Yan ◽  
Boyang Yu ◽  
Qiujuan Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Ischemia Reperfusion ◽  
Brain Infarction ◽  
Reaction System ◽  
Artery Occlusion ◽  
Dependent Manner ◽  
Endogenous Antioxidant

Oxidative stress is involved in the pathogenesis of ischemic neuronal injury. A Chinese herbal formula composed ofPoria cocos(Chinese name:Fu Ling),Atractylodes macrocephala(Chinese name:Bai Zhu) andAngelica sinensis(Chinese names:Danggui, Dong quai, Donggui; Korean name:Danggwi) (FBD), has been proved to be beneficial in the treatment of cerebral ischemia/reperfusion (I/R).This study was carried out to evaluate the protective effect of FBD against neuronal oxidative stressin vivoandin vitro. Rat I/R were established by middle cerebral artery occlusion (MCAO) for 1 h, followed by 24 h reperfusion. MCAO led to significant depletion in superoxide dismutase and glutathione and rise in lipid peroxidation (LPO) and nitric oxide in brain. The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group. All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg−1, p.o.). Moreover, cerebrospinal fluid sampled from FBD-pretreated rats protected PC12 cells against oxidative insult induced by 0.2 mM hydrogen peroxide, in a concentration and time-dependent manner (IC5010.6%, ET501.2 h). However, aqueous extract of FBD just slightly scavenged superoxide anion radical generated in xanthine–xanthine oxidase system (IC502.4 mg ml−1) and hydroxyl radical generated in Fenton reaction system (IC503.6 mg ml−1). In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

scholarly journals Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

2019 ◽  
pp. 1-8
Author(s):  
Leye Jonathan Babatola ◽  
Oluwakemisola B. Oshanimi ◽  
Olanrewaju M. Oluba ◽  
Lawrence Okoror ◽  
Adewale Agboola Odutuga
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Dependent Manner ◽  
Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

scholarly journals Experimental Assessment ofMoringa oleiferaLeaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential UsingIn VitroandIn VivoAssays

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Suaib Luqman ◽  
Suchita Srivastava ◽  
Ritesh Kumar ◽  
Anil Kumar Maurya ◽  
Debabrata Chanda
Keyword(s):  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Evaluation Studies ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Concentration Dependent Manner

We have investigated effect ofMoringa oleiferaleaf and fruit extracts on markers of oxidative stress, its toxicity evaluation, and correlation with antioxidant properties usingin vitroandin vitroassays. The aqueous extract of leaf was able to increase the GSH and reduce MDA level in a concentration-dependent manner. The ethanolic extract of fruit showed highest phenolic content, strong reducing power and free radical scavenging capacity. The antioxidant capacity of ethanolic extract of both fruit and leaf was higher in thein vitroassay compared to aqueous extract which showed higher potentialin vivo. Safety evaluation studies showed no toxicity of the extracts up to a dose of 100 mg/kg body weight. Our results support the potent antioxidant activity of aqueous and ethanolic extract ofMoringa oleiferawhich adds one more positive attribute to its known pharmacological importance.

scholarly journals In VitroAntioxidant and Cytotoxic Activities ofArnebia benthamii(Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Showkat Ahmad Ganie ◽  
Tanveer Ali Dar ◽  
Rabia Hamid ◽  
Ovais Zargar ◽  
Shayaq Ul Abeer ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Rat Liver Microsomes ◽  
Scavenging Activity

Arnebia benthamiiis a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study,in vitroantioxidant and anticancer activity of different extracts ofArnebia benthamiiwere investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest phenolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomesin vitro. In addition,Arnebia benthamiiextracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay.

scholarly journals Antioxidant and antiproliferative properties of Moringa oleifera Lam. leaf aqueous extract

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
D Athira Nair ◽  
T J James ◽  
S L Sreelatha ◽  
Bibu John Kariyil
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Dependent Manner ◽  
Standard Drug ◽  
In Vivo Analysis ◽  
Moringa Oleifera Lam

Moringa oleifera Lam. is a highly valued medicinal plant in India, especially Kerala. In the present study, antioxidant activity of aqueous extract of leaves of M. oleifera was determined both in-vitro and in-vivo. Male Wistar rats of 3 age groups- 6, 12, and 18 months old were used for in-vivo analysis. In vitro anti-proliferative effect of the extract was carried out in Dalton’s Lymphoma Ascites (DLA) Cells. LCMS-QTOF analysis of the extract was also done to determine the bioactive components present in the extract. Antioxidant activity of M. oleifera leaf showed an IC 50 value of 10.47 ?g/ml and whereas for standard drug, ascorbic acid, it was 19.52 ?g/ml. In-vivo analysis of lipid peroxidation showed a significant reduction of lipid peroxidation in the brains of 12 and 18-months old treated groups. Up to 75% mortality of DLA cancerous cells was observed in-vitro in different concentrations of M. oleifera leaf water extract in a dose-dependent manner, demonstrating its anti-proliferative property. LCMS-QTOF analysis revealed the presence of emodin-8-glucoside in the extract. Molecular docking analysis (Auto Dock Vina) of emodin-8-glucoside with six cancer related proteins showed highest binding affinity with AKT-1 with a binding score of -10.4 kcal/mol, also showed good affinity with NF-kB (p65), Stat-3, Bcl-2, Bcl-xl and c-FLIP. This study helps to choose healthy diet practices to overcome free radical onslaught and cancerous cell proliferation especially in the later stages of life. This can also pave way for the emergence of diet based therapeutic cure for cancer.

scholarly journals In vitro Inhibitory Effects of Cyperus esculentus L. (Tiger Nut) Tubers on some Enzymes Associated with Neurodegeneration and Iron- Induced Lipid Peroxidation in Rats’ Brain Tissue Homogenate

2020 ◽  
Vol 11 (2) ◽  
pp. 81-87
Author(s):  
Ayodeji Olabiyi ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Brain Tissue ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Tissue Homogenate ◽  
Cyperus Esculentus ◽  
Butyryl Cholinesterase ◽  
Radical Scavenging Ability ◽  
Brain Tissue Homogenate

This study investigated the inhibitory effect of Cyperus esculentus L. (raw and processed) tubers on Iron (Fe2+) induced lipid peroxidation and key enzymes linked with neurodegeneration in vitro. Ten grams of tiger nut tubers was weighed and added to 100 mL of distilled water (1:10 w/v), left for 24 h, filtered, centrifuged to obtain a clear supernatant and lyophilized. Activities of acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), Fe2+-induced lipid peroxidation as well as antioxidants status as indicated by 2,2-azino-bis (3-ethylbenthiazoline-6-sulphonic acid radical scavenging ability and Fe chelation were evaluated. Raw tiger nut extracts inhibited activities of AChE and BChE in the brain tissue of rats (0 – 11.54 mg /mL) with EC50 = 3.599 mg /mL and 2.71 ± 0.01 mg /mL respectively, and increased antioxidant activity when compared with the processed tuber. Inhibition on these enzymes as well as prevention of Fe2+ induced lipid peroxidation may be the possible mechanism of action by which tiger nut prevents neurodegeneration.

Sign in / Sign up

Export Citation Format

Share Document