scholarly journals Expression of miRNAs in Papillary Thyroid Carcinomas Is Associated withBRAFMutation and Clinicopathological Features in Chinese Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Yun Sun ◽  
Shuang Yu ◽  
Yuanyuan Liu ◽  
Fen Wang ◽  
Yujie Liu ◽  
...  
Keyword(s):  
Cervical Lymph Node Metastasis ◽  
Clinicopathological Features ◽  
Tnm Stage ◽  
Chinese Patients ◽  
Papillary Thyroid ◽  
Mutation Status ◽  
Expression Levels ◽  
Thyroid Carcinomas ◽  
Enhanced Expression

MicroRNAs (miRNAs) dysregulation has been shown to play a critical regulatory role in papillary thyroid carcinomas (PTCs).BRAFmutation is associated with poor clinicopathological outcomes in PTC. In order to identify a possible association between dysregulated miRNA expression andBRAFmutation as well as clinicopathological features in Chinese patients with PTC, we examined the expression levels of five reported dysregulated miRNAs (miRNA-221, miRNA-222, miRNA-146b, miRNA-181, and miRNA-21) and determinedBRAFmutation status in 52 patients with PTC and 52 patients with benign thyroid nodules (BTNs). The expression levels of all five miRNAs were significantly increased in PTC when compared to BTN. TheBRAFmutation occurred more frequently in PTC cases with advanced TNM stage. Importantly, miRNA-221, miRNA-222, miRNA-146b, and miRNA-181 expression levels were significantly higher in PTC patients withBRAFmutation. In addition, enhanced expression of miRNA-221 and miRNA-222 was found in patients with cervical lymph node metastasis and advanced TNM stage. Increased expression of miRNA-221 and miR-181 was evidenced in patients with larger tumors. These findings showed a potential role of this distinct profile of miRNAs in differentiating PTC from BTN.BRAFmutation might regulate or interact with miRNA in the pathogenesis and progression of PTC.

Quality Control and Telemedicine for BRAF V600E Mutations in Papillary Thyroid Carcinomas

2015 ◽  
Vol 4 (2) ◽  
pp. 12-30 ◽  
Author(s):  
Niki Margari ◽  
Abraham Pouliakis ◽  
Aris Spathis ◽  
Emmanouil Mastorakis ◽  
Efthymios Karakostas ◽  
...  
Keyword(s):  
Quality Control ◽  
Braf Mutation ◽  
Image Features ◽  
Classification And Regression Tree ◽  
Braf V600e ◽  
Papillary Thyroid ◽  
Cell Nuclei ◽  
Braf Mutations ◽  
Mutation Status ◽  
Thyroid Carcinomas

The assessment of BRAF V600E mutations is important for prognosis and treatment of Papillary Thyroid Carcinomas (PTC), the standard methods for their identification are molecular biology techniques. In this study, the potential of image morphometry applied to cell nuclei and sequentially the use of a Classification And Regression Tree (CART) is investigated, in order to: identify morphometric features useful to characterize BRAF mutations, and to eventually produce an algorithm identifying BRAF mutation status. The 140 studied cases had histological confirmation and known BRAF mutation status identified via real-time PCR. The analysis revealed that nuclear features contributing to BRAF mutation status identification via the CART model are related mostly to nuclear color. According to the results there is evidence that BRAF V600E mutations can be identified by measurable image features. Therefore, the proposed method is useful for quality control of BRAF V600E mutations on cytological slides, can serve as alternative to PCR method and may be used for remote assessment.

Clinicopathological features and immunohistochemical utility of NTRK-, ALK-, and ROS1-rearranged papillary thyroid carcinomas and anaplastic thyroid carcinomas

2020 ◽  
Vol 106 ◽  
pp. 82-92 ◽  
Author(s):  
Yui Nozaki ◽  
Hidetaka Yamamoto ◽  
Takeshi Iwasaki ◽  
Masanobu Sato ◽  
Rina Jiromaru ◽  
...  
Keyword(s):  
Clinicopathological Features ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

scholarly journals Are Long Noncoding RNAs New Potential Biomarkers in Gastrointestinal Stromal Tumors (GISTs)? The Role of H19 and MALAT1

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Giuseppe Badalamenti ◽  
Nadia Barraco ◽  
Lorena Incorvaia ◽  
Antonio Galvano ◽  
Daniele Fanale ◽  
...  
Keyword(s):  
Potential Role ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Pcr Analysis ◽  
Tumor Aggressiveness ◽  
Mutation Status ◽  
Expression Levels ◽  
Tissue Specimens ◽  
Selection Of

Long noncoding RNAs (lncRNAs) are emerging as key regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies described lncRNA alterations in patients with solid tumors. In particular, HOTAIR upregulation has been associated with tumor aggressiveness, metastasis, and poor survival in gastrointestinal stromal tumor (GIST) patients. We analyzed expression levels of other lncRNAs, H19 and MALAT1, in FFPE tissue specimens from 40 surgically resected and metastatic GIST patients, using real-time PCR analysis. H19 and MALAT1 were both upregulated in 50% of GIST patients. MALAT1 lncRNA expression levels seem to be correlated with c-KIT mutation status. The percentage of both H19 and MALAT1 upregulation was significantly higher in patients with time to progression (TTP) < 6 months as compared to patients with TTP > 6 months. The median TTP was significantly lower in patients with both H19 and MALAT1 lncRNA upregulation as compared to those with lncRNA downregulation. These data suggest a potential role for both H19 and MALAT1 lncRNAs as prognostic biomarker for the clinical selection of the best candidate to first-line treatment with imatinib.

scholarly journals Comparison of clinicopathological features in incidental and nonincidental papillary thyroid carcinomas in 308 patients

2017 ◽  
Vol 68 (3) ◽  
pp. 197-209 ◽  
Author(s):  
Nuray Can ◽  
Filiz Ozyilmaz ◽  
Mehmet Celik ◽  
Atakan Y. Sezer ◽  
Necdet Sut ◽  
...  
Keyword(s):  
Clinicopathological Features ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

No correlation between BRAFV600E mutation and clinicopathological features of papillary thyroid carcinomas in Taiwan

2005 ◽  
Vol 63 (4) ◽  
pp. 461-466 ◽  
Author(s):  
Rue-Tsuan Liu ◽  
Yi-Ju Chen ◽  
Fong-Fu Chou ◽  
Chun-Liang Li ◽  
Wei-Li Wu ◽  
...  
Keyword(s):  
Clinicopathological Features ◽  
Papillary Thyroid ◽  
Brafv600e Mutation ◽  
Thyroid Carcinomas

The role of tumor-infiltrating lymphocytes in papillary thyroid carcinomas

2011 ◽  
Vol 34 (9) ◽  
pp. 733-733 ◽  
Author(s):  
L. L. Cunha ◽  
F. A. Soares ◽  
J. Vassallo ◽  
L. S. Ward
Keyword(s):  
Tumor Infiltrating Lymphocytes ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas ◽  
Infiltrating Lymphocytes

scholarly journals Impairment of the p27kip1 function enhances thyroid carcinogenesis in TRK-T1 transgenic mice

2009 ◽  
Vol 16 (2) ◽  
pp. 483-490 ◽  
Author(s):  
Monica Fedele ◽  
Dario Palmieri ◽  
Gennaro Chiappetta ◽  
Rosa Pasquinelli ◽  
Ivana De Martino ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Double Mutant ◽  
Null Allele ◽  
Latency Period ◽  
Mutant Mice ◽  
Proliferation Index ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas

Impairment of the p27kip1 function, caused by a drastic reduction of its expression or cytoplasmic mislocalization, has been frequently observed in thyroid carcinomas. To understand the role of p27kip1 impairment in thyroid carcinogenesis, we investigated the consequences of the loss of p27kip1 expression in the context of a mouse modeling of papillary thyroid cancer, expressing the TRK-T1 oncogene under the transcriptional control of thyroglobulin promoter. We found that double mutant mice homozygous for a p27kip1 null allele (TRK-T1/p27−/−) display a higher incidence of papillary thyroid carcinomas, with a shorter latency period and increased proliferation index, compared with p27kip1 wild-type compounds (TRK-T1/p27+/+). Consistently, double mutant mice heterozygous for a p27kip1 null allele (TRK-T1/p27+/−) show an incidence of thyroid carcinomas that is intermediate between TRK-T1/p27−/− and TRK-T1/p27+/+ mice. Therefore, our findings suggest a dose-dependent role of p27kip1 function in papillary thyroid cancer development.

Clinical and Imaging Assessment of Cervical Lymph Node Metastasis in Papillary Thyroid Carcinomas

2010 ◽  
Vol 34 (7) ◽  
pp. 1494-1499 ◽  
Author(s):  
Yoon Jung Choi ◽  
Ji Sup Yun ◽  
Shin Ho Kook ◽  
Eun Choel Jung ◽  
Yong Lai Park
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cervical Lymph Node ◽  
Cervical Lymph Node Metastasis ◽  
Papillary Thyroid ◽  
Thyroid Carcinomas ◽  
Node Metastasis

Immunohistochemical markers in the diagnosis of papillary thyroid carcinomas: The promising role of combined immunostaining using HBME-1 and CD56

2013 ◽  
Vol 209 (9) ◽  
pp. 585-592 ◽  
Author(s):  
Adela Nechifor-Boila ◽  
Angela Borda ◽  
Geneviève Sassolas ◽  
Zakia Hafdi-Nejjari ◽  
Françoise Borson-Chazot ◽  
...  
Keyword(s):  
Papillary Thyroid ◽  
Thyroid Carcinomas ◽  
Immunohistochemical Markers
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